Improvement and Optimization of SELDI-TOF-MS

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1 14 1 Vol14 No1 010 Life Science Research Feb SELDI-TOF-MS 1 *, : (Surface-enhanced laser desorption / ionization-time of flight, SELDI-TOF-MS), SELDI-TOF-MS,,,, : SELDI-TOF-MS ; ; : R-331 : A : (010) Improvement and Optimization of SELDI-TOF-MS CHEN Ya-bin 1 LAN Xiao-peng * 1 Clinical Medicine SchoolFuzhou General HospitalFujian Medical UniversityFuzhou 35005FujianChina Institute of Laboratory MedicineFuzhou General Hospital of PLAFuzhou 35005FujianChina AbstractThe surface-enhanced laser desorption / ionization-time of flight SELDI-TOF-MSis one of the most promising approaches in proteome research Now it is widely used in some researchessuch as cancerinfection diseaseangiocardiopathynervous system diseaseand so on But some limitations in SELDI-TOF-MSfor examplethe effectivity and duplicate validation of the experimental result is low Further evaluation and optimization are called for before it's application in clinical diagnostics A lot of researches such as standardize the experimental processpretreatment of natural sample and dataimprove statistic method can optimize this technology remarkably Key wordsseldi-tof-ms improvementoptimization Life Science Research ~094 SELDI SELDI Surface-enhanced laser desorption / ionization-time SELDI of flighseldi-tof-ms 1 SELDI SELDI 11 SELDI : : : 06Z47 : cyb0807@yahoocomcn * Lanxp@sinacomcn

2 1 :SELDI-TOF-MS 91 [4] [5] [6] SELDI [7,8] [9] [10] [11] [1] 3 SELDI 31 1 SELDI DNA 5~ DNA 13 SELDI [14] 1 [15] 3 1~50 3 fmol 4 05~5 μl 5 6 SELDI [16,17] [18] SELDI [1~3]

3 SELDI Seam [] SELDI SELDI SELDI 18~ SELDI Table 1 Some quality control literatures of SELDI technology in recent years Emphasis Strict calibration Standardize the experimental process Pretreatment of natural sample Pretreatment of natural data Improve statistic method Standardize in different laboratories Quality control of samples with other type Others Amount % 18~1 90% Malyarenko [14] Hong [3] SELDI Bons [19] Albrethsen [16] CART SVM t Du [4] SELDI 60 min 60 min Banks [0] NP0 CAMDA 006 EDTA 7 6 IMAC-CU Aivado [1] 567% Semmes [5] 6 SELDI 57%~36% [19] [19] SELDI 3 [1] SELDI

4 1 :SELDI-TOF-MS 93 01% 40% 15%~36% SELDI / SELDI SLEDI SELDI (References): SELDI Guerreiro [6] SELDI 0 kd CM10 IMAC %~90% SPA serum amyloid alpha in renal cancer patients[j] CHCA Raymond [7] SELDI CM10 6~16 kd Q10 H4 1~6 kd IMAC-CU Chem ~10 kd [8] SELDI [9] SELDI 5 [1] KING FWHITE C NXIAO Xet al Using protemic approaches to identify new biomarkers for detection and monitoring of ovarian cancer[j] Gynecol Oncol [] FRAY FMARSHALL M D Sreum protein profiling by SELDI mass spectrometrydetection of multiple variants of J Urology [3] CUI JLIU YZHOU Het al Screening serum hepatocellular carcinoma-associated proteins by SELDI-based protein spectrum analysis[j] World J Gastroenterol [4] TERENCE P KALLEN C CPAK-CHEUNG Net al Serial analysis of proteomic signatures in pediatric patients with severe acute respiartory syndrome and viral load[j] Clin [5] MARSHALL JKUPECHAK PBURGESS Jet al Processing of serum proteins underlies the mass spectral fingerprinting of myocardial infarction[j] J Proteome Res [6] SIMONSEN A HmcGUIREA JPODUST V Net al Identification of a novel panel of cerebrospinal fluid biomarkers for Alzheimer s disease[j] Neurobiol Aging [7] CHO WYIP TYIP Cet al Identification of serum amyloid a protein as a potentially useful biomarker to monitor relapse of nasopharyngeal cancer by serum proteomic profiling [J] Clin Cancer Res [8] TSUKASA HYASUYOSHI NHITOSHI Set al CCL8 is SELDI a potential molecular candidate for the diagnosis of graftversus-host disease[j] Blood [9] YAMAYOSHI YWATANABE TTANABE Met al Novel application of ProteinChip technology exploring acute rejection markers of rat small bowel transplantation[j] Transplanta- SELDI tion SELDI [J] World J Gastroenterol SELDI [10] ZHEN GZHU YZHONG Xet al Discovering differential protein expression caused by CagA-induced ERK pathway activation in AGS cells using the SELDI-ProteinChip platform [11] MarBEATH GSCHREIBER S L Printing protein as

5 microarrays for high-throughput function determination [J] ionization mass spectrometry[j] Clin Chem Science [1] [1] AIVADO MSPENTZOS DALTEROVITZ Get al Optimization and evaluation of surface-enhanced laser desorption/ioni [J] CHEN XuedongWANG Ruo-guangYOU Zhao-linget al Detection zation time-of-flight mass spectrometryseldi-tof-mswith of anti-nasopharyngeal carcinoma water-soluble proteins from reversed-phase protein arrays for protein profiling[j] Clin pberetima asiatica with protein chip[j] Chinese Journal of Chem Lab Med Otorhinolaryngology-Skull Base Surgery [] SEAM NGONZALES D AKERN S Jet al Quality con trol of serum albumin depletion for proteomic analysis[j] Clin Chem [J] [3] HONG HDRAGAN YEPSTEIN Jet al Quality control ZHENG Yan-yingWANG Ya-jieKANG Xi-xiong The and quality assessment of data from surface-enhanced laser quality control and standardization of surface-enhanced laser desorption / ionization SELDItime-of Flight TOFmass desorption / ionization tim e-of-flight inass spectrometry [J] spectrometry MS[J] BMC bioinformatics Chinese Journal of Laboratory Medicine [4] Suppl S5 DU PKIBBE W ALIN S M Improved peak detection in [14] MALARENKO D ICOOKE W EADAM Bet al mass spectrum by incorporating continuous wavelet transform- Enhancement of sensitivity and spectrometric records for serum peptides using time-series analysis techniques[j] Clin Chem [15] WANG Y FCHEUNG T HWANG V Wet al Protein profiling of cervical cancer by protein-biochipsproteomic scoring to discriminate cervical cancer from normal cervix[j] Cancer Lett [16] ALBETHSEN JBOGEBO ROLSEN Jet al Preanalytical and analytical variation of surface-enhanced laser desorptionionization time-of-flight mass spectrometry of human serum[j] Clin Chem Lab Med [17] SEAM NGONZALES D AKERN S Jet al Quality control of serum albumin depletion for proteomic analysis[j] Clin Chem [18] ALBETHSEN J Reproducibility in protein profiling by MALDI-TOF mass spectrometry[j] Clin Chem [19] BONS J Ade BOER Dvan DIEIJEN-VISSER M Pet al Standardization of calibration and quality control using surface enhanced laser desorption ionization-time of flight-mass spectrometry[j] Clinica chimica acta [0] BANKS R ESTANLEY A JCAIRNS D Aet al Influences of blood sample processing on low molecular-weight proteome identified by surface-enhanced laser desorption/ based pattern matching[j] Bioinformatics [5] SEMMES O JFENG ZADAM Bet al Evaluation of serum protein profiling by surface-enhanced laser desorption/ ionization time-of-flight mass spectrometry for the detection of prostate canceri Assessment of platform reproducibility [J] Clin chem [6] GUERREIRO NGOMEZ-MANCILLA BCHARMONT S Optimization and evaluation of surface-enhanced laserdesorption / ionization time-of-flight mass spectrometry for protein profiling of cerebrospinal fluid[j] Proteome Sci [7] RAYMOND S PARNOUD L FJOLAN D Get al Salivary protein / peptide pro-filing with SELDI-TOF-MS[J] Ann N Y Acad Sci [8] [J] WANG De-xiCHEN Yi-niYE Zhi-haiet al Preliminary study the difference of protein fingerprints in the pleural effusion and discuss their clinical significance[j] Journal of Guangdong College of Pharmacy [9] ROLLIN DWHISTLER TVERNON S D Laboratory methods to improve SELDI peak detection and quantitation [J] Proteome Sci