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1 6, 4, CHIN ESE BIODIV ERSIT Y 6 (4) : , November, 1998 Ξ 6 Ξ Ξ (, ) Villani F ( Institute of Agriforestry, CNR 0510 Porano ( TR), Italy) 8 6 ( Per22 Got22 Est22 Gpi22 Idh21 Idh22 ) ( %, 2. 54, ),,89. 2 %,10. 8 %, ,,,, A research on genetic variation of Castanea mollissima at six isozyme loci/ Zhang Hui 1), Liu Liu 1), Villani F 2) Abstract Allozyme genetic variation at six loci ( Per22, Got22, Est22, Gpi22, Idh21, Idh22 ) in eight pop2 ulations of C. mollissim a was invested using starch gel electrophoresis. The result showed very high genetic variation in the populations with the proportion of polymorphic loci of % in the average expected het2 erozygosity of In general, about % of the genetic variation was attributed within populations, while the remaining % among populations. The genetic distance ranged from There ex2 isted obvious difference, especially in a few locus. The primary result indicted the high level of genetic varia2 tion and difference could be closely related to the long gene migration flow which attributed to the diversity of its habitat, the outcrossing2winded character and so on. Key words Castanea mollissim a, Allozyme, Genetic variation Author s address 1) Jiangsu Institute of Botany, Chinese Academy of Sciences, Nanjing ) Institute of Agriforestry, CNR 0510 Porano ( TR), Italy ( Castanea mollissi m a) (N , 22 ),,,,,, 6 [1, ], [2,, ] [3 ], ( Castanea sativa) Villani F 50, [4 6 ],,, : ; : Ξ, Ξ Ξ

2 4 : 6 283, 1 111,8 5 ( 1), 30 ( 5 8 ), 5, Table 1 Geographical sites of the eight sampled populations of C. No. Local population the Yangtze River valley the Yangtze River valley Southwest China Southwest China Southeast China North China North China Northwest China Location Lishui, Jiangsu Hefei, Anhui Yiliang, Yunnan mollissim a (m) North latitude East longitude Altitude l Yunnan Ξ / / / Liancheng, Fujian (1) Xinyi, Jiangsu(1) (2) Xinyi, Jiangsu(2) Ξ Ξ Northwest China / / / : Ξ The germplasm introduced from Yunnan by Nanjing Botanical Garden Ξ Ξ The germplasm introduced from Northwest China by Nanjing Botanical Garden 112 Villani F [4 ],, 100 ml, M Tris g,ed TA g, PVP 4 %,D TT g, l, p H : PER, GPI(A &B ) [7 ], GO T ( Poulic ) [8 ], IDH ( TC,p H 6. 5 ) [9 ] 113 Wendel [10 ], 2, 0. 01,, ( P, A, He)

3 , 19, 2 5 Gpi22,, 2 Table 2 Allelic gene frequencies of C. mollissim a Population Loci Allele Per22 A B C Got22 A B C D Gpi22 A B C Est22 A B C Idh21 A B C ID H22 A B C Ξ 1 Population numbers correspond to those in Table 1, Got22, 3 4, 2 3, Per22, 3 4 Per22 ( A ) ( P), A, He ( ), A , ; He ( 4), ( 8),,, F2 ( F2Statistics) Hardy2 [11 Weinberg ], 3,, Fst 6 Fst , %, % Nei (1972) [12 ] I D, 6, : D D, U PGMA,, 1,

4 4 : 6 285, 2 8, I 3 6 Table 3 Comparison of genetic diversity level between C. mollissim a and C. sativa at six loci Parameter He Mean Loci [ 6 ] C. sativa C. mollissim a Difference value Per Got Gpi Est Idh Idh He P % % % A F st Fig. 1 U PGMA dendrogram based on Nei s genetic distance of eight populations tested , , 97 %,,, ( Castanea dentata), He , P % [13 ] ;, [6 ] 3 ( ) 6,, Got22 Idh22, ( He) ,, He P A ( ),,,, 6, 6,

5 286 6 [ Hamrick & Godt ],,,, 200 m,,,,, Hamrick & Gotd, P , ; He ,0. 418, %,10. 8 %,,,,,,,, 1,,.. :,1988 2,..,1988,15 (2) : ,..,1990,5 (3) : Villani F,Benedetti S, Paciucci M et al. Genetic variation and differentiation between natural populations of chestnut ( Castanea sativa. Mill) from Italy. In : Hattemer H and Fineschi S (eds. ), Biochemical M arkers in the Population Genetics of Forest T ree, Netherlands : SPB Academy Publishing, 1990, Villani F, Pigliucci M, Benedetti S et al. Genetic differentiation among Turkish chestnut ( Castanea sativa Mill. ) populations. Heredity, 1991, 66 : Villani F, Pigliucci M, Lauteri M et al. Congruence between genetic, morphometric and physiological data on differentiation of Turish chestnut ( Castanea sativa Mill. ). Genome, 1992, 35 : Ashton G C, Braden W H. Serum globulin polymorphism in mice. A ust. J. Biol. Sci. 1961, 14 : Poulic M D. Starch gel electrophoresis in a discontinuous system of buffers. N ature (London), Muller2storck C. Genetic differences between tolerant and sensitive beech ( Fagus sylvatica L. ) in an envi2 ronmentally stressed adult forest stand. Silvae Genetica, 1985, 34 (6) : Wendel J F, Weecen N F. Visualization and interpretation of plant isozymes. In : Solitis E D, Soltis P S (eds. ), Isozymes in Plant Biology,Portlant : Dioscorides Press, Wright S. The interpretation of population structure by F2statistics with special regard to system of mating. Evolution, 1965,19 : Nei M. Genetic distance between population. A mer. N at.,1972,106 : Hung H W,Dane F, Allozyme diversity in Chinese, Seguin and American chestnut ( Castanea spp. ) Theor. A ppl. Genet., 1994, 8 : Hamrick J L, Godt M J W. Allozyme diversity in plant species. In : Brown A H, Clegg M T, Kahler A L (eds. ), Plant population genetics, breeding and genetics resources, Sunderland,Mass : Sinauer,1990,43 63