An Outline of New Molecular Mechanisms16S rrna Methyltransferases Acquired by Pathogenic Bacteria Yoshichika ARAKAWA 2010 1, 2 NDM-1 -β -MBL β - NDM-1 MBL 16S RNA 16S-RMTase 3 16S RNA 30S 16S rrna 16S RNA 16S-RMTase 30S RNA 16S rrna 30S 16S-RMTases RsmG YhiQRsmJ AG AG 30S 16S rrna 1405 G 1408 A KgmB Streptomyces tenebrariuskama Streptomyces tenjimariensis 2003 16S-RMTase AG 4 16S-RMTases S-rRNA N 5 AG 16S- RMTases 1405 G N7 1408A N1 S 0466-8550 65 Department of Bacteriology, Nagoya University Graduate School of Medicine (65 Tsurumai- cho, Showa-ku, Nagoya, Aichi)
16S rrna 16S rrna 30S 16S rrna A-site 1405 1408 1405G AG MIC, 512gml 1408A AG 16S rrna 21 S proteins 30S 16S-RMTases 250 290 1408A NpmA KamA 219 1405G 16S-RMTases 7 16S rrna 1405G Pseudomonas aeruginosa 6 RmtA P. aeruginosa
1405G 16S-RMTases 16S-RNTases DB RmtA ArmA RmtB RmtC NpmA RmtD 2002 1996 C. freundii 2003 2002 S. marcescens 2004 2003 2006 2005 RmtD2 2009 2007 RmtE RmtF 2002 1997 2006 2003 2010 P. mirabilis E. coli E. coli 2003 bla CTX-M-3, bla TEM-1, Galimand, M, et al. aacc2, aada2, dfra2, sul1, bla NDM-1, bla CTX-M-15, bla SHV-12, 2004 Doi Y, et al. 2006 Wachino J, et al. 2007 Doi J, et al. 2011 Tijet N, et al. 2003 Yokoyama K, et al. 2007 Wachino J, et al. 2010 Davis MA, et al. bla TEM-1, aada2, qepa bla NDM-1, bla CTX-M-15, bla CMY-58, bla kpc-2, bla OXA-10, bla VIM-1, apha1, bla NDM-1, bla SPM-1 cat, dfra12, aada2, sul1 aph3 -Ia, apha7, stra, strb 2012 La Reunion 2012 aac6 -Ib 2011 K. pneumoniae Galimand M, et al. P. aeruginosa C. freundii E. coli K. pneumoniae, S. enterica P. stuartii Providencia sp. P. aeruginosa A. baumannii ArmA E. colisalmonella enterica S. marcescens, E. coli, P. mirabilis, K. pneumoniae, ArmA E. coli E. aerogenes, C. freundii E. cloacae, M. morganii, L. adecarboxylata, P. mirabilis E. coli P. aeruginosa P. aeruginosa E. coli K. pneumoniae S. enterica Virchow La Reunion
RmtA 1405G Serratia marcescens 7 16S-RMTases RmtB RmtB2 RmtB3 RmtA RmtB 1405G Proteus mirabilis 8 2010 NDM-1 RmtC RmtA RmtC 1405G P. aeruginosa 9 RmtD1RmtD2 2 MBL SPM-1 RmtD RmtA RmtD Escherichia coli 10 RmtA RmtE K. pneumoniae 11 2012 ICAAC RmtF RmtA RmtF 1996 Citrobacter freundii bla CTX-M-3 16S-RMTase 2002 12 Golebiewski Klebsiella pneumoniae ArmA 13 ArmA Acinetobacter RmtB 16S-RMTases 1 16S-RMTase 16S-RMTase 3 1405G 1408A AG AG MIC, 512gml AG ArmA RmtBRmtC NDM-1 SPM-1 MBL
ArmA RmtB CMY CTX-M E. coli RmtB ArmA ArmA 2005 RmtC ArmA 16S-RMTase IS rmta 14 rmtb Tn3 IS26 rmtc ISEcp1rmtD orf494arma ISCR1npmA IS26 DNA IMP-1 MBL IMP-1 1991 S. marcscens 15 TN9106 IMP ienem ossible act MBL D2 MBL IS MBL CAZ SHV- ESBL CMY CAZ MIC MBL MBL MBL 16, 17 SMA disk IMP-1 VIM-2 MBL 17 230
IMP-1 AmpC NDM-1 CMY CTX-M- β- SMA CAZ MEPM disk SMA disk 5 8mm Modified SMA-disk NDM-1 Klebsiella β - K. pneumoniae TEM-1 18 TEM-1 Klebsiella IS SHV-1 TEM-1 K. pneumoniae LEN-1 Klebsiella oxytoca β- K. oxytoca β - C K. oxytoca β - K. pneumoniae A β - 19 A Klebsiella oxytoca RbiA K1 β- 20 1990 ESBL A β - C K. pneumoniae 1991 β - C MIR-1 MOX-1 21, 22 MOX-1 CMY-1 CMY-2 CMY CMY-8 AmpC CMY DHA AmpC MBL C β - C β - 22 KPC 2000 1995
Toho-1 β - 23 CTX-M-1 CTX-M- 2CTX-M-9 CTX-M ESBL 24 2000 O25 : H4MLST ST131 25 GyrA ParC DNA QRDR MexAB-OprM rmtb QepA rmtb 26 RmtB RmtB QepA B GBS 2000 GBS GBS PCG MIC 0.12 PBP2X PCG MIC MIC 27 PRGBS disk 28 PRGBS PRGBS 29 30 10 31, 32 48
1984-1986 K. oxytoca- 1986-1990 MBL 2000-1991-1992 S. marcescens 1992-1994 1995-1997 S. marcescensmbl plasmid1993-1997 K. oxytoca- 1995-1996 GBS PRGBS 2005-2012 PCRIMP-1 1995-1996 ESBL1994-2012 -1995-2000 C- AmpCCMY 2004-2005 CTX-MESBLMBL 1999-2009 2003 ESBLMBL 16S rrna methyltransferase 2002-2012 AmpC- 2001-2004 RmtB2003-2004 2003-2004 16S rrna 2003-2010 2006-2008 2008-2009 MBL 2008-2010 - RR-- - - RR-- Klebsiella - C - MOX-1 SBTCPZ- PBP2X PRGBS CTX-M-2ESBL 2002-2004 GBS 2007-2012 CTX-M-64 2008-2009 2006-2012 IMP-1 IMP-1 IMP-1 PCR SHV-24, TEM-91 Toho-1 1 AmpCCMY CTX-MESBL RmtA GES-3GES-4 RmtC, NpmA - JANIS AmpCRmtB AAC6 -Iad RmtA QepA CTXLVFX O25 : H4-ST131 VIM-2MBL CTX-M-2ESBL Proteus mirabilis Acinetobacter baumannii PRGBS PRGBS CTX-M-64 YJ Park JH Liu
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