Pharmacokinetics of Florfenicol in the Chinese Mitten-handed Crab Eriocheir sinensis Dose Effect

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ĒΓαβριήλ Κοτζιάς
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1 Chinese Journal of Zoology ~ * Eriocheir sinensis 3P mg / kg 3P97 3 T 1 /2α T 1 /2β h h CLs ml / kg h T 1 /2α T 1 /2β h h CLs ml / kg h T 1 /2α T 1 /2β h h CLs ml / kg h HPLC S948 A Pharmacokinetics of Florfenicol in the Chinese Mitten-handed Crab Eriocheir sinensis Dose Effect CHEN Ling-Zhen 1 YANG Hong-Sheng 2 3 WU Guang-Hong 2 3 * CHEN Hai-Qian 4 XU Xing-Lian 1 1 Key Laboratory of Meat Processing and Quality Control Ministry of Education Nanjing Agricultural University Nanjing 2 Aquatic Products Analysis & Testing Center of Jiangsu Province Nanjing Fishery Products Quality Supervision and Testing Center Nanjing Ministry of Agriculture Nanjing 4 Department of Food Science and Nutrition Nanjing Normal University Nanjing China Abstract The pharmacokinetics of florfenicol FF after intramuscular injection was investigated in the Chinese Mitten-handed Crab Eriocheir sinensis at 17. Hemolymph muscle and liver samples were obtained and analyzed by a high-performance liquid chromatography with fluorescence detection method and the corresponding pharmacokinetic parameters were calculated with the 3P97 software. The florfenicol level in hemolymph and muscle reached maximum momentarily while that in liver reached a maximum then declined at No. BM No. BM * ghwu2007@ 163. com chenlingzhen2005@ yahoo. com. cn

2 4 103 three administration doses mg / kg. The kinetic profile of florfenicol in hemolymph muscle and liver was conformed to a two-compartment model. The important parameters were listed. When three doses were injected the absorption half-lives T 1 /2α and the elimination half-lives T 1 /2β in hemolymph were and h respectively and the total body clearance CLs were and ml / kg h respectively the T 1 /2α and T 1 /2β in muscle were h and h respectively and the CLs were and ml / kg h respectively the T 1 /2α and T 1 /2β in liver were h and h respectively and the CLs were and ml / kg h respectively. Key words Eriocheir sinensis Florfenicol HPLC Pharmacokinetics Florfenicol 1 -NO ± 10 g 17 ± 1 2 Sus Ovis aries Procavia apensis Gallus gallus domestica Biziura lobata 5 Beckman 21R ph METTER TOLEDO MP225 Buchi R215 Salmo salar 6 Ictalurus g Punctatus 7 Waters 2695 Waters % Penaeus chinensis 8 Pelodiscus sinensis 9 > 98% K 2 HPO 4 KH 2 PO 4 Millipole g ml ph Agilent TC-C18 Eriocheir sinensis 250 mm 4. 6 mm 5 μm / = 45 /55 v / v 225 nm nm μl 0. 6 ml / min

3 104 Chinese Journal of Zoology mg / kg min h ml 7. 5 ml g r / min μg / kg 4 5 min g 1. 6 Origin g 20 ml - 3P r / min Practical Pharmacokinetic Program 10 min 20 ml ml ml 10 ml ml C 18 SPE C 18 SPE 3 ml 2 ml / min C 18 SPE 3 ml 3 ml 2. 2 y mg / L 6 ml 40 y = x r = ml μm 2 2 g R / N N R MDL = 3C N / R 2 ml MDL μg / kg C 2 g 10 ml μg / kg r / min μg / kg min 10 ml 92. 6% % 93. 3% RSD ml μm g g 100 ml 500 mg / L mg / L 20 μl x ~ mg / L 5. 27% 6. 67% 3. 51%

4 4 105 Fig. 1 1 FF Liquid chromatogram of florfenicol A B 4 h A Chromatogram of standard sample μg / L B Chromatogram of liver sample at 4 h post-injection. 2 Fig. 2 n = 5 Florfenicol concentration-time curves in various organs or tissues of Eriocheir sinensis at different administration doses

5 106 Chinese Journal of Zoology Table 1 Pharmacokinetic parameters of florfenicol Table 3 Pharmacokinetic parameters of florfenicol in hemolymph of Eriocheir sinensis at in liver of Eriocheir sinensis different administration doses Administration doses mg / kg at different administration doses Parameter Administration doses mg / kg A mg / ml Parameter α / h A mg / ml B mg / ml α / h β / h B mg / ml Vc ml / kg T 1 /2α h β / h T 1 /2β h Vc ml / kg K 21 / h T 1 /2α h K 10 / h T 1 /2β h K 12 / h K 21 / h AUC 0 ~ mg / ml h K 10 / h CLs ml / kg h K 12 / h A α B AUC 0 ~ mg / ml h β Vc T 1 /2α CLs ml / kg h T 1 /2β K K 10 K AUC 0 - CLs α β the hybrid rate constants for distribution and elimination phases A B the zero time drug concentration intercepts of the biphasic disposition curve Vc the apparent volume of the central compartment. T 1 /2α T 1 /2β the absorption half-life and the elimination half-life K 12 K 21 the first-order rate constants for drug distribution between the central and peripheral compartment A mg / ml α / h B mg / ml β / h Vc ml / kg T 1 /2α h T 1 /2β h K 21 / h K 10 / h K 12 / h AUC 0 ~ mg / ml h CLs ml / kg h Origin 2 1 min K 10 the first-order rate constant for disappearance of drug from the central compartment AUC 0 - the area under the concentrationtime curve from zero to infinity CLs the total body clearance. The same is applied to the following table. 2 3P97 Table 2 Pharmacokinetic parameters of florfenicol in muscle of Eriocheir sinensis at different administration doses 1 ~ mg / kg Administration doses mg / kg Parameter T 1 /2α T 1 /2β h h CLs ml / kg h T 1 /2α T 1 /2β h h CLs ml / kg h T 1 /2α T 1 /2β h h CLs ml / kg h 3 AUC

6 mg / ml h mg / ml h mg / ml h r < r > mg / kg AUC 20 mg / kg h Varma 20 Adama 21 Bos tarurs ~ h Liu h Afifi 23 Shen T 1 /2α T 1 /2β r > T 1 /2α r > T 1 /2α T 1 /2β AUC r > ~ h Byung Canis familiaris h mg / kg 3. 2 MIC Shojiaee 26 MIC 11 Vibrio anguillarum Aeromonas 19 salmonicida Pasteurella piscida Edwardsiella tarda 9 MIC 0. 5 ~ 0. 8 μg / ml mg / kg 10 mg / kg 0. 8 μg / ml 1 h 1 ~ h 20 mg / kg

7 108 Chinese Journal of Zoology μg / ml 4 h 5 mg / kg 10 mg / kg 20 mg / kg 4 h 0. 8 μg / ml 1 Voorspoels J D'Haese E De Craene B A et al. Pharmacokinetics of florfenicol after treatment of pigs with single oral or intramuscular doses or with medicated feed for three days. Vet Record Ali B H Al-Qarawi A A Hashaad M. Comparative plasma pharmacokinetics and tolerance of florfenicol following intramuscular and intravenous administration to camels sheep and goats. Vet Res Commun El-Banna H A. Pharmacokinetics of florfenicol in normal and Pasteurella-infected Muscovy ducks. British Poultry Science Afifi N A EI-Sooud K A. Tissue concentration and pharmacokinetics of florfenicol in broiler chickens. British Poultry Science Koc F Ozturk M Kadioglu Y et al. Pharmacokinetics of florfenicol after intravenous and intramuscular administration in New Zealand White rabbits. Research in Veterinary Science Bernt M Tor E H Kanval J V et al. Single dose pharmacokinetic study of florfenicol in Atlantic salmon Salmo salar in seawater at 11. Aquaculture Wrzesinski C Crouch L Gaunt P et al. Florfenicol residue depletion in channel catfish Ictalurus punctatus Rafinesque. Aquaculture / Fangke D Cao J Ma L et al. Pharmacokinetics and tissue residues of difloxacin in crucian carp Carassius auratus after oral administration. Aquaculture / Wu G Meng Y Zhu X et al. Pharmacokinetics and tissue distribution of enrofloxacin and its metabolite ciprofloxacin in Chinese mitten - handed crab Eriocheir sinensis. Analytical Biochemistry Rigos G Alexis M Andriopoulou A et al. Pharmacokinetics and tissue distribution of oxytetracycline in sea bass Dicentrarchus labrax at two water temperatures. Aquaculture Haug T Hals P A. Pharmacokinetics of oxytetracycline in Arctic charr Salvelinus alpinus L. in fresh water at low temperature. Aquaculture / Varma K J Adams P E Powers T E et al. Pharmacokinetics of florfenicol in veal calves. J Vet Pharmacol Ther Adams P E Varma K J Powers T E et al. Tissue concentrations and pharmacokinetics of florfenicol in male veal calves given repeated doses. Am J Vet Res Liu J Fung K F Chen Z et al. Pharmacokinetics of florfenicol in healthy pigs and in pigs experimentally 8. infected with Actinobacillus pleuropneumoniae Antimicrobial Agents and Chemotherapy Afifi N A Aboel-Sooud K A. Tissue concentrations and pharmacokinetics of florfenicol in broiler chickens. British Poultry Science Shen J Hu D Wu X et al. Bioavailability and pharmacokinetics of florfenicol in broiler chickens. Pharmacol Ther J Vet Park B K Lim J H Kim M S et al. Pharmacokinetics of

8 4 109 florfenicol and its metabolite florfenicol amine in dogs. Research in Veterinary Science Shojaee-Allabadi F Lees P. Antibiotic treatment for animals effect on bacterial population and dosage regiment optimization. Int J Antimicorob Agents Fukui H Fujihara Y Kano T. In vitro and in vivo antibacterial activities of florfenicol a new fluorinated analog of thiamphenicol against fish pathogents. Fish Pathology Samuelsen O B Bergh O Ervik A. Pharmacokinetic of florfenicol in cod Gadus morhua and in vitro antibacterial actibity against Vibrio anguillarim. Dis Aquat Organ Inglis V Richards R H. The in vitro susptibility of Aeromonas salmonicida and other fish pathogenic bacteria to 29 antimicrobial agents. Journal Fish Diseases Kim E H Yoshida T Aoki T. Detection of R plasmid encoded with resistance to florfenicol in Pasteurella piscicida. Fish Pathol MRL EB / OL. www. hc-sc. gc. ca / english / index. html International Conservation Workshop for the Chinese Giant Salamander ~ Helen Meredlith

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