Real - time monitoring of tumor response to photothermal / photodynamic therapy by functional magnetic resonance imaging

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1 Journal of Fuzhou University Natural Science Edition Vol 43 No 5 Oct 2015 DOI /issn T2 R445 2 A Real - time monitoring of tumor response to photothermal / photodynamic therapy by functional magnetic resonance imaging CAO Jianbo FU Guifeng ZHANG Fan LIU Gang Center for Molecular Imaging and Translational Medicine School of Public Health Xiamen University Xiamen Fujian China Abstract This paper assess the long - term curative effect of tumor after different treatment strategies Compared with photodynamic therapy or photothermal therapy alone the combined therapy showed a significantly synergistic effect which was demonstrated by functional MRI The results provide a direct non - invasive method for in vivo monitoring of the treatment response to nanoparticle - mediated PTT which can be used to guide cancer therapy to improve potential clinical outcomes Keywords functional MRI photothermal therapy photodynamic therapy real - time monitoring 0 photothermal therapy PTT photodynamic therapy PDT DWI 8 - DWI e6 G0 - PEG - Ce gangliu cmitm@ xmu edu cn

2 5 723 PEG nano - graphene oxide ngo RGO e6 chlorin e6 Ce6 630 ~ 700nm e T1 MTT μl ~ h μg ml - 1 GO - PEG - Ce6 24 h 24 h 5 mg L - 1 MTT 20 μl 4 h 100 μl DMSO 10 min 490 nm OD = / 100% MTT 96 5 μmol Ce6 GO - PEG - Ce h nm 10 min 0 3 W cm nm 5 min 0 05 W cm W cm nm 10 min 0 05 W cm nm 5 min MTT 4 h 100 μl DMSO 10 min 490 nm OD Balb /C 16 ~ 18 g 4T μl 20 PBS GO - PEG - Ce μl PBS 24 h nm 10 min 1 W cm nm 5 min 0 2 W cm W cm nm 10 min 0 2 W cm nm 5 min 10% 4 μl g MRI MRI d 5 9 4T Bruker 94 /20 USR Germany 4 cm RF 1H 75 /40 Bruker Germany MRI 1 T2 TurboRARE TR /TE = /33 ms mm 1 0 mm field of view FOV = 40 mm 40 mm 2 SE - DWI B 800 s mm - 2 TR /TE = /27 ms mm 1 0 mm FOV = 40 mm 40 mm 1 5 4% hematoxylin - eosin staining platelet endothelial cell adhesion molecule - 1 PECAM - 1 / CD31 CD31 1 h Cy3 IgG immunoglobulin G http / /xbzrb fzu edu cn

3 a MTT GO - PEG - Ce μg ml h 80% 200 μg ml % 1 b GO - PEG - Ce6 4 h 40% 30% 20% 1 Fig 1 Cell toxicity tests and photothermal therapeutic effect a T2 2 b ADC ADC apparent diffusion coefficient ADC DWI ADC PBS MRI ADC 14 d 4 ~ 5 2 a ADC 7 d ADC GO - PEG - Ce6 24 h Fig 2 2 In vivo MRI representative T2WI ADC - map and MRI evaluating tumor response after PTT http / /xbzrb fzu edu cn

4 ~ 49 MRI ADC 3 d 14 d 3 d ADC a T2 relative tumor volume PBS 4 ~ 5 3 d 7 d 14 d 3 d 3 b ADC ADC 1 d 3 d ADC 3 d ADC ADC PBS ADC 2 4 Fig 3 3 Effect of different treatment on the tumor HE CD31 4 PBS HE CD31 HE CD31 4 HE CD31 Fig 4 HE stained images and CD31 stained images of different treatment 13 ADC ADC 1 d ADC http / /xbzrb fzu edu cn

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