Journal of Jiangsu University Medicine Edition. NSCLC Let7 NSCLC

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1 Journal of Jiangsu University Medicine Edition Vol. 21 No. 4 Jul mirnalet7 C-myc mirnalet7 let7 C-myc NSCLC let7 C-myc 68 NSCLC NSCLC 20 NSCLC Let7 NSCLC 39. 7% 75. 0% χ 2 = P < C-myc NSCLC 54. 4% 25. 0% χ 2 = P < Let7 C-myc P > let7 C-myc NSCLC r = P < let7 2 χ 2 = P = Cmyc 2 χ 2 = P = Let7 C- myc NSCLC mirnalet7 C-myc R A Expression and clinical pathological significance of mirnalet7 C-myc in non-small cell lung cancer XU Chuan-hai 1 LAN Jian-yun 1 YAN Xiao-ou 2 SHEN Cheng-hong 2 YAN Xiao-mei 2 FENG Yi-zhong 3 1. Department of Pathology the First People's Hospital of Yancheng Yancheng Jiangsu Department of Oncology the First People' s Hospital of Yancheng Yancheng Jiangsu Department of Pathology and Physiopathology School of Medicine Soochow University Suzhou Jiangsu China Abstract Objective To study the effects of micrornalet7 C-myc on carcinogenesis and progression in non-small cell lung cancer NSCLC. Methods In-situ hybridization was applied to detect the expression of let7 in 68 cases of non-small cell lung cancer and 20 cases of adjacent lung tissues while SP immunohistochemistry was adopted to measure C-myc in these specimens. Results The positive rate of let7 in NSCLC group was 39. 7% which was lower than 75. 0% in control group χ 2 = P < while the positive rate of C-myc in NSCLC group was 54. 4% which was higher than 25. 0% in control group χ 2 = P < The positive rate of let7 and C-myc had no relation with clinic-pathological parameter P > there was an obvious negative correlation between let7 and C-myc r = P < The 2-year survival rate of positive let7 is higher than that of the negative group χ 2 = P = There had no statistical significance between positive and negative groups of C-myc for two-year survival χ 2 = P = Conclusion The lower expression level of let7 and high expression level of C- myc were related with NSCLC and further research were needed. Key words non-small cell lung cancer micrornalet7 C-myc in-situ hybridization immunohistochemistry YK

2 4. mirnalet7 C-myc mirnalet7 - SP RNA 4 μm 2000 Reinhart 3% 10 min 1 mir- 10% 10 min 4 NAlet7 C-myc 68 NSCLC mirnalet7 C-myc 20 min SP 10 min DAB 3 3 NSCLC PBS NSCLC let7 C-myc NSCLC 68 NSCLC 5 cm < ~ < 10% 0 10% ~ 25% 1 26% ~ 50% 2 51% ~ 75% 3 > 75% cm 31 > 3 cm 37 = 25 < 3 3 ~ > 6 UICC 7 TNM TNMⅠ 37 TNMⅡ 19 TNMⅢ SPSS let7 C-myc NSCLC χ 2 let7 C-myc NSCLC Spearman let7 C-myc 1. 2 Kaplan-Meier Has-let-7a Exiqon C-myc Log-rank Invitrogen P < S-P DAB PBS 2 ISH 2. 1 mirnalet7 4% SABC-POD let7 NSCLC let NSCLC % mirna % 16 h 37 χ 2 = P < Let7 60 min SABC DAB NSCLC

3 P > C-myc NSCLC % χ 2 = P < C-myc 2 C-myc NSCLC P > % 1 1 Tab 1 Let7 C-myc NSCLC Relationship between clinical pathological parameters and expression of mirnalet7 C-myc in non-small cell lung cancer n < > 3 cm cm P-TNM Ⅰ Ⅱ Ⅲ Let7 C-myc % χ 2 P % χ 2 P > > > > > > > > > > > > > > > > let7 C-myc NSCLC Kaplan-Meier C-myc let % let7 Log-rank let % let7 2 r = P < χ 2 = P = let7 C-myc 2 let NSCLC 14 3 C-myc 2 54 NSCLC 4 C-myc % χ 2 = 2. 47

4 4. mirnalet7 C-myc 347 P = Fig 3 2 let Weidhaas 4 let7 let7 let7 let7 let7 2 Relationship between expression of let7 and 2-year survival rate let7 C-myc MC29 5 myc C-myc 20% C-myc 6 Zajac-Kaye 7 C-myc 4 C-myc 2 Fig 4 Relationship between expression of C-myc and 2-year survival rate 2006 Hsu C-myc mirna 18 ~ % C-myc RNA C-myc NSCLC mirnalet7 mirna Reinhart 1 P < C. elegans TNM let Takamizawa 3 P > let7 143 C-myc let7 COX C-myc let7 3'UTR let7 let7 A549 let Akao 9 let7 let7 A549 myc 2009 Chang 10 C-myc let7 NSCLC myc mirna let7 let7 NSCLC let7 C-myc let7 let7 C-myc NSCLC let7 NSCLC NSCLC 2 P < let

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