NF-κB TSLP. Expression of TSLP and NF-κB in patients with adenomyosis

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1 NF-κB doi /j. issn X NF- κb R A X NF-κB SABC NF-κB p65 ELISA 20 NF-κB p65 P < NF-κB p65 P < NF-κB p65 P < P < 0. NF-κB -κb p65 NF-κB p65 Expression of and NF-κB in patients with adenomyosis LIU Ying REN Yun-Qing XUE Li-Ping LI Xiao-Yan SONG Bin-Yu. Shanxi Medical University Taiyuan China Abstract Objective To investigate the role of and NF-κB in the pathogenesis of adenomyosis. Methods Immunohistochemistry was employed to detect the expression of and NF-κB p65 in the endometrial glandular cell and stromal cell of 35 adenomyosis patients and 20 hysteromyoma patients as control and analyze the correlation of the two proteins. The concentration of was measured by specific ELISA in the serum of healthy subjects besides of the patients with adenomyosis and hysteromyoma. Results and NF-κB p65 were both positively expressd in the glandular cells and stromal cells of ectopic and eutopic endometrium of patients with adenomyosis their expression was significantly higher than that of the control group P < and the expression of and NF-κB p65 in ectopic endometrium was significantly higher than that in eutopic endometrium P < There was positive correlation between and NF-κB p65 in ectopic endometrium. The concentration of was higher in the serum of patients with adenomyosis. Conclusion The is highly expressed in endometrial glandular and stromal cells of patients with adenomyosis and there is higher level of in serum and there is correlation between the expression of and NF-κB in ectopic endometrium. Key words NF-κB p65 Adenomyosis Adenomyosis AM 30 ~ 50 Yoko Urata 1 IL-1β Th @ 163. com 2 Lee 3 NF-κB NF-κB 4 NF-κB

2 NF-κB NF-κB 400 Image-ProPlus MOD x ± s 1. 7 SPSS ~ 58 t ± Pearson P < P < ~ ± X ± ± ± ± ~ ± min 1. 0 cm 1. 0 cm 1. 0 cm P < P < % 24 h 2. 2 NF-κB p65 NF-κB p65 4 μm HE ml NF-κB p ± ± 3 ml 30 min r /min 10 min EP - 20 DAB AR1022 ELISA 1. 4 SABC DAB mol /L PBS 1. 5 ELISA ABC-ELISA P < P < ± ± NF-κB p65 1 SABC IgG- SV0002 Tab. 1 Expression of in group of patients with adenomyosis and hysteromyoma MOD x ± s Groups n Glandular cell Stromal cell Ectopic endometrium of AM ± ± Eutopic endometrium of AM ± ± Endometrium of Hysteromyoma Control ± ± Note vs control 1 P < ectopic endometrium vs eutopic endometriuml 2 P < gladular cell vs stromal cell 3 P < P = P =

3 NF-κB NF-κB p ± ± NF-κB p P < Rs = P = Rs = P < P = P = NF-κB p65 NF-κB p ± ± NF-κB p65 Rs = P = NF-κB p P < NF-κB p65 Rs = P = Rs = P P < = NF-κB p NF-κB p65 NF-κB p65 Rs = ± ng /ml ± ng /ml P = P = P = ± ng /ml P = NF-κB 400 p Fig. 1 Expression of in endometrium of patients Fig. 2 Expression of NF-κB p65 in endometrium of patients with adenomyosis and hysteromyoma IHC with adenomyosis and hysteromyoma IHC Note A. Ectopic endometrium of patients with AM B. Eutopic Note A. Ectopic endometrium of patients with AM B. Eutopic endometrium endometrium of patients with AM C. Endometrium of patients of patients with AM C. Endometrium of patients with with hysteromyoma G. Glandular cells S. Stromal hysteromyoma G. Glandular cells S. Stromal cells Scale bar cells. 25 μm. 2 Tab. 2 NF-κB p65 Expression of NF-κB p65 in group of patients with adenomyosis and hysteromyoma Groups n Glandular cell MOD x ± s Stromal cell Ectopic endometrium of AM ± ± Eutopic endometrium of AM ± ± Endometrium of Hysteromyoma Control ± ± Note vs control 1 P < ectopic endometrium vs eutopic endometrium 2 P < gladular cell vs stromal cell 3 P < P = P = Fig. 3 NFκB p65 Correlation between and NF-κB p65 in ectopic endometrial glandular cells

4 NF-κB p65 Fig. 4 Correlation between and NF-κB p65 in ectopic endometrial stromal cells 5 PDGF COX-2 11 Bcl-2 survivin Fig. 5 Serum levels of in every group 13 Note 1. Adenomyosis 2. Hysteromyoma 3. Healthy. vs Hysterom- NF-κB yoma *. P < vs Healthy #. P < IL ERK p38mapk NF-κB CD18 ICAM-1 8 Yoko Urata ICAM-1 NF-κB p50 RelA p65 p50 DNAκB p65 C NF-κB IκB 5 NF-κB p65 15 Nie 16 NF-κB p65 NF-κB p65 NF-κB p65 NF-κB MMP-9 17 COX-2 NF-κB 1994 Friend

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