Advance in Research on Antimicrobial Property and Application of ε-pl

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1 ε (10, 56 ) 1, 1 2, : ε- (ε-pl), :ε- ; ; Advance in Research on Antimicrobial Property and Application of ε-pl LI Kun-lun 1, LI Jiang-kuo 1, ZHANG Peng 2, ZHANG Ping 1 (1. Tianjin Key Laboratory of Postharvest Physiology and Storage of Agricultural Products, National Engineering and Technology Research Center for Preservation of Agriculture Products, Tianjin , China 2. College of Food Science, Shenyang Agricultural University, Shenyang , China) Abstract: As a kind of natural food preservative, ε-poly-l-lysine (ε-pl) on its physical chemistry characters, antimicrobial activities and mechanism were introduced and its application and development were reviewed in this paper. Key words: ε-pl; preservative; antimicrobial activities; application :TS202.3 :A : (2010) ε- (ε- PL) ph MIC 100 μg/ml ε- PL FDA ph ε- PL 1977 S Shima H Sakai 1 ε- PL ε- ε- PL Streptomyces albulus L- 1.1 S.albulus ε- PL 25~35 L- α β :

2 ε- PL ε-pl H HN (CH 2 ) 4 CH CO OH ε-pl n 150 μg/ml NH 2 50 μg/ml 1.2 ph ε-pl 150 μg/ml, ph 5~8 ε-pl 50 μg/ml 50 μg/ml [2] S Shima Escherichia coli K-12 ε- 1.3 PL 9 L- ε-pl α [3] min min ε-pl J Hiraki ph 3.0 ε- PL E.coli ph 5.0~8.0 ε- PL 25~50 μg/ml ph μg/ml [2] 1.4 ε-pl 3 ε- PL ph ε-pl [1] ph L- 36% ε-pl ε- PL 0.21~0.56 S Shima ε-pl [3], ε-pl ε-pl Donnan α ε-pl [1] 2 ε- PL ε- PL J Hiraki ε-pl J Hiraki ε-pl MIC 1 [2] 12

3 ε- :bxyjg@163.com 1 ε-pl MIC/μg ml -1 ph AspergillusnigerIFO TrichophytonmentagrophytesIFO CandidaacutusIFO PhaffiarhodozymaIFO PichiaanomalaIFO P membranaefaciensifo0577 <3 5.0 RhodotorulalactaseIFO SporobolomycesroseusIFO1037 <3 5.0 Saccharomycescerevisiae ZygosaccharomycesrouxiiIFO GeobacillusstearothermophilusIFO BacilluscoagulansIFO B subtilisiam1069 <3 7.3 ClostridiumacetobutylicumIFO LeuconostocmesenteroidesIFO LactobacillusbrevisIFO L plantarumifo MicrococcusluteusIFO StaphylococcusaureusIFO StreptococcuslactisIFO RaoultellaplanticolaIFO Campylobacterjejuni EscherichiacoliIFO PseudomonasaeruginosaIFO Salmonellatyphymurium ε-pl MIC 100 μg/ml ε-pl MIC 3.2 ε-pl Salmonella typhimurium ε-pl 000~5 000 μg/g 90 1 Subchronic 10~500 μg/g A Fukutome toxicity ε-pl [4] μg/g 14 C ε-pl ε-pl μg/g ADME ( ε-pl μg/g ) ε-pl ε-pl, J Hiraki 5~50 μg/g, ε-pl 300 g ( 50 μg/g ε-pl) 60 kg [5] ε-pl 15 mg ε-pl ε-pl 14 5 g/kgε-pl / 5 min 3.3 3~6 h ε-pl ph 1.25~2.5 g/kg ε-pl 13

4 Chisso Corporation ε- PL ε- PL ph mg/kg ε-pl ε-pl ph 5~8 ε- PL ε-pl 3.4 ε-pl Ho Yu- [2] Ting ε-pl ε-pl ph 7 1 mol/l NaCl Listeria monocytogens E. ε-pl coli O-157: H7 Salmonella typhimurium Vibrio choleraevibrio parahae molyticus Staphylococcus aur [6] -eaus 3.5 ε-pl MIC * (E.coli) MIC/mg L -1 ck min min min 50 ph ε-pl MIC MIC/mg L -1 ph Bacillus subtilis Bacillus cereus Escherichia coli Staphylococcus aureus <6.3 ε-pl J Hiraki [9] 14 C- ε- PL ADME ε-pl 168 h X ε-pl ADME ε- PL 500 mg/kg 1 000~5 000 mg/kg 10~500 mg/kg ε- PL 5~50 mg/kg ε-pl [7] 4.2 ε-pl J Hiraki [8] ε-pl 4 ε- PL ε-pl [6] 4.1 Y Ho ε- PL Maillard reaction J Hiraki [8] S Shima [3] Sunsoft SE-11 Q-18S ε-pl ph K Neda1999 ε- NaCl 1.0 mol/l PL ε-pl 14

5 ε- :bxyjg@163.com ε-pl ε- PL 4.3 ε- PL ε-pl ε-pl ε-pl ε-pl Y Kito [10] ε- PL ε-pl 4 50% IC 50 : ε-pl μmol/l : <ε-pl 25~ μmol/lα-pl44 [1] Mariya Marudova, Alistair J Macdougall, Stephen G Ring. Physicochemical studies of pectin/poly-l-lysine gelation [J]. Carbohydrate 7.6 μmol/l<α-pl 11 Research,2004,339: μmol/l ε-pl IC 50 α-pl44 [2] Hiraki J. ε -Polylysine, its development and utilization [J].Fine 7.6 μmol/l Chem,2000,29: ε-pl [3] Shima S, Matsuoka H, Iwamoto T, et al.antimicrobial action of ε-pl ε-poly-l-lysine[j]. Antibiot 1984, 37: α-pl ε-pl [4] Fukutome A, Kashima M, Aiuchi M. Acombined chronic toxicity and carcinogenicity study of polylysine powder in rats by peroral α- ε-pl dietary administration[j].the Clinical Report,1995,29: [5] Hiraki J, TIchikawa, Ninomiya S I, et al. Use of ADME studies Y to confirm the safety of ε- polylysine as a preservative in food[j]. Regulatory Toxicology and Pharmacology,2003,37(2): Kito ε- PL [6] Ho Y, Ishizaki S, Tanaka M. Improving emulsifying activity of 15 mg/kg ε-polylysine by conjugation with dextran through the maillard reaction[j]. Food Chem, 2000,68: ε-pl 2~3 h ε- PL ε- [7] Santini M, Cesare C, Pietro L. Polylysine induces changes in PL p<0.05 [10] membrane electrical properties of K562 cells [J].Journal of ε-pl Biomedical Materials Research,1997,35(2): [8] Hiraki J. Basic and applied studies on ε-polylysine [J]. Antibact Antifungal Agents, 1995, 23: [9] Hiraki J, Ichikawa T, Ninomiya S I, et al. Use of ADME studies 5 ε- PL to confirm the safety of ε-poly-l-lysine as a preservative in food[j]. Regulatory Toxicol Pharm, 2003,37: ε-pl [10]Kito Y, Hiramot S, Murao M, et al. ε-polylysine inhibits pancreatic lipase activity and suppresses postprandial hypertriacyl- ε-pl glyceridemia in rats[j]. J Nutr, 2003,133: ε- : PL 15