252 42 2 2013 3 JOURNAL OF HYGIENE RESEARCH Vol. 42 No. 2 Mar. 2013 1000-8020 2013 02-0252-06 ghrelin 1 100050 ghrelin 879 8 ~ 10 BMI % BF TC TG LDL-C HDL-C ghrelin ghrelin BMI % BF P < 0. 0001 HDL-C P < 0. 0001 TC ghrelin BMI % BF TG HDL-C TC LDL-C ghrelin BMI % BF R151. 41 R195. 1 A Relationships between serum ghrelin and lipid profile and serum leptin adiponectin and insulin among children in Beijing China DU Songming HU Xiaoqi LIU Ailing ZHANG Qian PAN Hui MA Guansheng Institute of Nutrition and Food Safety Chinese Center for Diseases Control and Prevention Beijing 100050 China Abstract Objective To study the relationship between serum ghrelin and lipid profile and serum leptin adiponectin and insulin among children in Beijing. Methods A total of 879 pupils aged 8-10 years were randomly selected. Height body weight waist circumference body mass index BMI body fat percentage %BF blood lipid serum leptin insulin adiponectin and ghrelin were measured in all samples. Results Ghrelin associated negatively with body weight waist circumference BMI %BF serum leptin level and positively with HDL-C. Factor analysis has shown five factors which were sufficient to explain correlations between variables-body composition and leptin dyslipidemia glucose metabolism and adiponectin. Conclusion Serum ghrelin level correlated strongly with body weight waist circumference BMI %BF and leptin Our finding suggested significantly increased leptin levels following decreased ghrelin levels could mostly disordered adipose metabolism at early obesity in children. Key words children and adolescents obesity ghrelin leptin insulin E-mail dusm9709@ 126. com 1 E-mail mags@ chinacdc. cn
2. ghrelin 253 ghrelin - growth hormone secretagogue receptor GHSR percentage body fat %BF = - / growth hormone GH "100% fat free mass FFM = 0. 406 cm 2 / + 0. 360 kg + 5. 580 ghrelin ghrelin cm /100 + 0. 56 = 1 = 0-6. 48 2 = 2 + 2 0. 5 ghrelin 3 1. 2. 4 ghrelin ghrelin 1 1. 1 8-80 2 2 1. 2. 4. 2 TC 72 10 TG LDL-C 20 HDL-C FBG 900 8 ~ 10 7080 7080 1. 2. 4. 3 ghrelin enzyme-linked immunoadsordent assay ELASA 1. 2 1. 2. 1 1. 2. 5 Seca WGOC model 890 Hamburg 5 2 0. 1kg 2 0. 1cm 1. 3 BMI = kg / m 2 1. 2. 2 ± quartile QR 2 cm t 0. 1cm 2cm χ 2 ghrelin 4 < Q 1 Q 1 ~ Q 2 ~ Q 3 ~ 4 % BF 1. 2. 4. 1 12h 3000r / min 1ml Automatic Analyzer HITACHI Phoenix Biotech EpiData 1. 2. 3 ghrelin RJL2System 101 USA 50kHz 800μA 1 -
254 42 P < 0. 05 459 52. 2% 420 SAS 8. 2 47. 8% BMI 2 2. 1 900 879 17. 6% 1 % BF P < 0. 01 19. 4% 28. 3% 15. 0% 1 Table 1 The characteristics of subjects n % % cm kg cm BMI % BF 8 51 19. 6 31. 4 136. 6 ± 6. 5 2 36. 2 ± 9. 7 2 64. 6 ± 11. 6 19. 2 ± 4. 1 25. 4 ± 8. 5 9 206 17. 0 35. 0 140. 4 ± 6. 5 39. 8 ± 10. 7 67. 7 ± 11. 6 19. 9 ± 4. 1 26. 9 ± 7. 9 10 202 21. 8 20. 8 144. 5 ± 6. 6 41. 5 ± 11. 8 67. 9 ± 12. 0 19. 6 ± 4. 2 26. 9 ± 7. 7 456 19. 4 2 28. 3 2 141. 8 ± 7. 1 2 40. 1 ± 11. 2 2 67. 4 ± 11. 8 2 19. 7 ± 4. 2 2 26. 7 ± 7. 9 2 8 67 16. 4 14. 9 135. 1 ± 6. 4 32. 5 ± 7. 4 60. 0 ± 8. 5 17. 7 ± 3. 1 15. 2 ± 10. 8 9 193 15. 0 19. 2 139. 1 ± 6. 8 35. 6 ± 8. 8 61. 7 ± 9. 5 18. 2 ± 3. 5 17. 0 ± 10. 5 10 160 14. 4 16. 9 143. 8 ± 6. 6 38. 7 ± 9. 5 63. 5 ± 9. 4 18. 6 ± 3. 5 18. 2 ± 9. 6 420 15. 0 17. 6 140. 3 ± 7. 3 36. 3 ± 9. 1 62. 1 ± 9. 4 18. 3 ± 3. 5 17. 2 ± 10. 3 P < 0. 0001 2 t P < 0. 001 2. 2 ghrelin ghrelin P = 0. 0007 TG TC HDL-C LDL-C P = 0. 6367 P = 0. 1670 2 2 Table 2 Plasma lipid and adipokines concentration in study group TG TC HDL-C LDL-C 8 4. 7 ± 0. 4 1. 0 ± 0. 7 4. 1 ± 0. 6 1. 4 ± 0. 3 2. 4 ± 0. 6 9 4. 7 ± 0. 4 1. 1 ± 0. 6 3. 9 ± 0. 5 1. 4 ± 0. 3 2. 2 ± 0. 5 10 4. 6 ± 0. 4 1. 1 ± 0. 9 3. 9 ± 0. 6 1. 5 ± 0. 3 2. 2 ± 0. 6 4. 7 ± 0. 4 1. 0 ± 0. 7 4. 0 ± 0. 6 1. 4 ± 0. 3 2. 2 ± 0. 6 8 4. 6 ± 0. 3 1. 0 ± 0. 5 3. 8 ± 0. 5 1. 4 ± 0. 3 2. 1 ± 0. 5 9 4. 6 ± 0. 3 1. 1 ± 0. 6 3. 9 ± 0. 6 1. 4 ± 0. 3 2. 3 ± 0. 5 10 4. 5 ± 0. 4 1. 1 ± 0. 5 3. 9 ± 0. 6 1. 4 ± 0. 3 2. 3 ± 0. 6 4. 6 ± 0. 3 1. 1 ± 0. 5 3. 9 ± 0. 6 1. 4 ± 0. 3 2. 2 ± 0. 6 μg / ml ghrelin 2 8 1. 94 2. 93 2. 40 5. 80 17. 45 10. 09 4. 21 3. 36 9 2. 14 2. 08 3. 07 7. 06 15. 61 11. 57 3. 70 2. 39 10 1. 74 2. 35 2. 09 7. 42 17. 39 11. 56 3. 88 2. 53 1. 93 2. 31 2. 66 6. 96 16. 46 11. 42 3. 81 2. 49 8 1. 77 2. 00 1. 52 4. 69 16. 07 12. 10 3. 83 1. 79 9 2. 13 2. 55 2. 35 6. 10 17. 46 10. 33 3. 27 2. 28 10 2. 29 2. 39 2. 38 5. 43 15. 03 10. 74 3. 60 2. 01 2. 15 2. 39 2. 25 5. 77 16. 84 10. 95 3. 50 2. 14 2 P < 0. 001
2. ghrelin 255 2. 3 ghrelin C 3 ghrelin 4 ghrelin BMI % BF P < HDL-C 0. 0001 P < 0. 0001 TC P = 0. 0297 TG LDL- Table 3 3 ghrelin Body composition of the study group by ghrelin quartile ghrelin / % % cm kg cm BMI %BF < Q1 223 105 /118 18. 8 27. 4 141. 3 ± 7. 2 39. 4 ± 9. 9 66. 7 ± 10. 4 19. 5 ± 3. 8 30. 7 ± 6. 3 Q 1 ~ 225 112 /113 16. 0 27. 1 141. 1 ± 7. 7 39. 1 ± 11. 7 65. 6 ± 11. 7 19. 3 ± 4. 2 29. 7 ± 7. 2 Q 2 ~ 219 113 /106 18. 7 21. 5 141. 3 ± 7. 4 38. 2 ± 10. 4 64. 6 ± 11. 3 18. 9 ± 3. 9 29. 3 ± 7. 1 Q3 ~ 212 129 /83 15. 6 16. 5 140. 5 ± 6. 6 36. 4 ± 9. 3 62. 5 ± 10. 3 18. 2 ± 3. 6 27. 7 ± 7. 1 P 0. 0489 0. 0489 0. 6127 0. 0114 0. 0007 0. 0029 < 0. 0001 ghrelin ghrelin Table4 ghrelin 4 ghrelin Plasma lipid and adipokines level of the study group by ghrelin quartile TG TC HDL-C < Q 1 1. 73 0. 71 4. 61 ± 0. 28 1. 13 ± 0. 83 3. 93 ± 0. 58 1. 35 ± 0. 28 Q 1 ~ 3. 12 0. 67 4. 59 ± 0. 38 1. 05 ± 0. 61 3. 85 ± 0. 55 1. 39 ± 0. 28 Q 2 ~ 4. 21 0. 49 4. 64 ± 0. 39 1. 03 ± 0. 47 4. 00 ± 0. 59 1. 44 ± 0. 27 Q 3 ~ 5. 70 1. 31 4. 59 ± 0. 38 1. 02 ± 0. 63 3. 98 ± 0. 54 1. 47 ± 0. 28 P < 0. 0001 0. 4977 0. 2313 0. 0297 < 0. 0001 ghrelin LDL-C μg / ml < Q 1 2. 30 ± 0. 60 2. 60 2. 87 6. 16 11. 09 16. 83 10. 99 Q 1 ~ 2. 19 ± 0. 56 2. 06 2. 33 2. 52 6. 26 15. 20 10. 46 Q 2 ~ 2. 26 ± 0. 58 1. 80 2. 01 1. 96 4. 48 17. 53 11. 54 Q 3 ~ 2. 21 ± 0. 57 1. 93 1. 97 1. 16 3. 22 16. 78 11. 48 P 0. 1985 0. 0038 2 < 0. 0001 2 0. 0439 2 2 ghrelin 2. 4 ghrelin ghrelin 5 BMI %BF 5 1 2 TG ghrelin HDL-C3 TC LDL-C4 ghrelin 42. 2% 14. 0% 9. 6% 7 8. 1% 6. 2% 80. 1% 3 ghrelin 9 CUMINGS 10 adipokines -6 resistin 6 8 ~ 10 ghrelin ghrelin 8 ghrelin ghrelin
256 42 Table 5 5 Factor-analysis of the study group according to body composition plasma lipid and adipokines 1 2 3 4 5 0. 761-0. 074-0. 115-0. 003-0. 039 0. 954 0. 169 0. 106 0. 077-0. 089 0. 895 0. 239 0. 177 0. 100-0. 103 BMI 0. 862 0. 260 0. 193 0. 106-0. 103 %BF 0. 655 0. 396 0. 229 0. 037-0. 118 0. 056-0. 025-0. 014 0. 945 0. 016 TC 0. 072-0. 122 0. 975 0. 018 0. 017 TG 0. 128 0. 781 0. 213 0. 010-0. 066 HDL-C - 0. 198-0. 836 0. 246 0. 011 0. 074 LDL-C 0. 207 0. 213 0. 895 0. 010-0. 021 0. 370 0. 445 0. 110 0. 459-0. 120 0. 556 0. 454 0. 174 0. 173-0. 189-0. 202-0. 142 0. 010-0. 012 0. 964 % 42. 2 14. 0 9. 6 8. 1 6. 2 % 42. 2 56. 2 65. 8 73. 9 80. 1 ghrelin ghrelin 11 ghrelin 1 KOJIMA M HOSODA H DATE Y. Ghrelin is a growth-hormone releasing acylated peptide from stomach BMI % BF J. Nature 1999 402 656-660. ghrelin BMI 2. DVC ghrelin ghrelin NPY mrna AgRP mrna 12-13 BMI ghrelin BMI TG LDL-C ghrelin ghrelin BMI %BF Diabetes Rev 2006 2 19-28. ghrelin 7 CUMMINGS D E. Ghrelin and the short- and long-term β regulation of appetite and body weight J. Physiol 14 ghrelin Behav 2006 89 71-84. 2 15 8 HOSODA H KOJIMA M KANGAWA K. Biological physiological and pharmacological aspects of ghrelin J. J Pharmacol Sci 2006 100 5 398-410. ghrelin 9 NAKAZATO M MURAKAMI N DATA Y et al. A ghrelin role for ghrelin in the central regulation of feeding J. 16 Nature 2001 409 6817 194-198. J. 2009 45 4-6. 3. ghrelin J. 2012 33 10 1168-1169. 4. 7 ~ 18 ghrelin J. 2007 36 5 35-38. 5 ghrelin. J. 2004 25 2 97-102. 6 BULC O C FERREIRA S R GIUFFRIDA F M et al. The new adipose tissue and adipocytokines J. Curr 262
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