1. 1 Glycyrrhiza glabra L. Sprague-Dawley Glycyrrhiza inflate Batal Glycyrrhiza uralensis

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1 42 2 Vol 42 No Journal of Jiangxi Normal University Natural Science Mar α- 18β- * α- 18β- Gly SD α-Gly 18β-Gly α-Gly 18β-Gly ALT AST γ-gt LDH 18β-Gly 18α-Gly 18β-Gly TBIL TBA 18α- 18β- R A DOI /j cnki issn β-Gly α-Gly 18β-Gly Alcoholic Liver Disease ALD α-Gly 18β-Gly 40% 1 L 14% 8% 1 ALD 2 ALD 1 ALD 1 1 Glycyrrhiza glabra L Sprague-Dawley 8 SPF Glycyrrhiza inflate Batal Glycyrrhiza uralensis Fisch SCXK α α-Gly 18β- 18β-Gly 7600S 18α-Gly 18β-Gly Synergy HT Bio-TEK H A zhaoyany606@ 163 com

2 2 18α- 18β- 167 CHE CK TP EZⅢ ALB GLO TBIL TBA α 18β = OGTT h 50% 50 mg -1 S 18α 18β = 2 0 g kg min mg > 99% AUC mg SPSS 19 0 γ- one-way ANOVA x ± s α-Gly 18β-Gly α-Gly 18β-Gly ALT AST ALP γ-gt CHE SD 1 10 ALT AST ALP γ-gt CHE 10 Normal α-Gly 18β-Gly Model Silymarin ALT ALP 18α-Gly 18β-Gly AST γ-gt α- 8 2 ALT γ-gt Gly 18β-Gly 0 9% 0 01 AST CHE % 10 ml ALT γ-gt CHE kg - 1 ig 40% 10 ml kg AST α-Gly 4 6 ALT γ-gt 18β-Gly mg kg AST ALP CHE mg kg ALT AST CHE 100 g 0 9% 0 01 ALP γ-gt 0 3 ml ALT AST γ-gt 14 h 10% 3 ml kg ALP CHE 2 h rpm a ~ e 10 min LDH SD LDH α-Gly 18β-Gly ALT AST γ- γ- LDH GT ALP LDH 0 10 LDH f

3 CK CK 0 01 ALT AST γ-gt LDH 18α-Gly 18β-Gly 18β-Gly 18α CK 0 05 Gly 18β-Gly CK α-Gly 18β-Gly 1 g 18α-Gly 18β-Gly 5 5 N Normal M Model P Positive a ~ g 18α-Gly 18β-Gly * p < 0 05 **p < 0 01 #p < 0 05 ##p < α-Gly 18β-Gly α-Gly 18β-Gly 0 01 GLO p < α-Gly 18β-Gly 18α-Gly 18β-Gly 8 2 TP 0 01 ALB GLO TP ALB α-Gly TP ALB * p < 0 05 **p < 0 01 #p < 0 05 ##p < α-Gly 18β-Gly α-Gly 18β-Gly Gly 18α-Gly 18β-Gly α-Gly 18β-Gly 3 18α-Gly 18β-Gly TBIL TBA α-Gly 18β-Gly AUC TBA TBIL p > a TBA 0 01 p < β-

4 2 18α- 18β- 169 p > 0 05 AUC 0 05 AUC α-Gly 18β-Gly AUC b 18β-Gly 3 * p < 0 05 **p < 0 01 #p < 0 05 ##p < α-Gly 18β-Gly * p < 0 05 **p < 0 01 #p < 0 05 ##p < α-Gly 18β-Gly 2 a 4 b 4 18α-Gly 18β-Gly 18α- Gly 18β-Gly 18α- AST ALT Gly 18β-Gly ALT AST ALP γ-gt CHE LDH CK TP ALB γ-gt LDH 18β-Gly GLO TBIL TBA GLO CHE CHE TBIL TBA CHE LDH CK ALB 18α-Gly 18β-Gly TP GLO 18α-Gly 18β-Gly Louvet A Mathurin P Alcoholic liver disease mechanisms of injury and targeted treatment J Nat Rev Gas-

5 tro Hepat O'shea R S Dasarathy S Mccullough A J Alcoholic liver disease J Hepatology Hayashi H Sudo H Economic importance of licorice J Plant Biotechnol-Nar Ming L J Yin A Therapeutic effects of glycyrrhizic acid J Nat Prod Commun J Negishi M Irie A Nagata N et al Specific binding of glycyrrhetinic acid to the rat liver membrane J Biochim Biophys Acta Li Jianyuan Cao Hongyan Liu Ping et al Glycyrrhizic acid in the treatment of liver diseases Literature review J Biomed Res Int Guo Xiaoling Liang Bo Wang Xuewei et al Glycyrrhizic acid attenuates CCl 4 -induced hepatocyte apoptosis in rats via a p53-mediated pathway J World J Gastroenterol Jeong H G You H J Park S J et al Hepatoprotective effects of 18β-glycyrrhetinic acid on carbon tetrachlorideinduced liver injury inhibition of cytochrome p450 2E1 expression J Pharmacol Res Ukil A Biswas A Das T et al 18β-Glycyrrhetinic acid triggers curative Th1 response and nitric oxide up-regulation in experimental visceral leishmaniasis associated with the activation of NF-κB J Journal of Immunology Wu Xudong Zhang Luyong Gurley E et al Prevention of free fatty acid-induced hepatic lipotoxicity by 18beta-glycyrrhetinic acid through lysosomal and mitochondrial pathways J Hepatology Xiao Yichuan Xu Jingwei Mao Chaoming et al 18β-glycyrrhetinic acid ameliorated propionibacterium acnes-induced a- cute liver injury through inhibition of MIP-1α J Journal of Biological Chemistry Zeng Chunxiang Yang Qing Hu Qin A comparison of the distribution of two glycyrrhizic acid epimers in rat tissues J European Journal of Drug Metabolism & Pharmacokinetics Zou Qiaogen Wei Ping Li Jing et al Simultaneous determination of 18α- and 18β-glycyrrhetic acid in human plasma by LC-ESI-MS and its application to pharmacokinetics J Biomedical Chromatography Bmc J J Ding Wenxiao Zhang Qiang Dong Yanbin et al Adiponectin protects the rats liver against chronic intermittent hypoxia induced injury through AMP-activated protein kinase pathway J Scientific Reports Rogers C Q Ajmo J M You M Adiponectin and alcoholic fatty liver disease J Iubmb Life Yamauchi T Kamon J Minokoshi Y et al Adiponectin stimulates glucose utilization and fatty-acid oxidation by activating AMP-activated protein kinase J Nat Med The Effect of Different Proportion of 18α- 18β- Glycyrrhizic Acid on the Liver Function in Alcoholic Liver Disease Rat SUN Xiaoke YANG Sa MENG Xiangbo ZHOU Chengyan ZHAO Yanyan * College of Pharmaceutical Science Key Laboratory of Pharmaceutical Quality Control of Hebei Province Hebei University Baoding Hebei China Abstract The objective is that investigating protective effects of different proportion of 18α- and 18β- glycyrrhizic acid Gly on alcoholic liver injury in rats Healthy male rats are randomly divided into equally ten groups respectively normal control group saline model group saline positive drug control group silymarin 7 given drug groups different ratios of 18α-Gly and 18β-Gly group and ratios are At the fourth week blood are collected to determine serum ferment and proteins total bilirubin and acid bile Blood glucose is measured The results show that different proportions of 18α- and 18β-glycyrrhizic acid all effectively reduced ALT AST γ-gt and LDH activity The alcoholic liver injury and myocardial injury can be better improved when the ratio is less than 4 6 And the serum protein level in alcoholis liver injury rat is improved when the ratio is greater then 8 2 The level of TBIL and TBA And is attenuated when the ratio is less than 4 6 And the proportion is less then 4 6 that obviously improves the ability of regulating the blood glucose of rats with alcoholic liver injury It can be applied to selection of new drug research and clinical treatment with a basic experiment data Key words alcoholic liver disease liver function different proportion 18α-glycyrrhizic acid and 18β-glycyrrhizic acid