Effect of ultra son ic2a ssisted extraction on polysacchar ide structure from C oprinus com a tus character ized by FT IR and AFM

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1 Chinese Journal of B iop rocess Engineering Vol. 8 No. 1 Jan doi: / j. issn , 2, 1, 1, 1, 1 (1., ; 2., ) :, (WCP) (UCP) WCP UCP % % Sephadex G 200 WCP UCP, WCP3 1 UCP3 1 2, ( FTIR) (AFM ) : WCP3 1 UCP3 1, WCP3 1 UCP3 1, C O C O ;WCP3 1, UCP3 1,, : ; ; : Q336 : A : (2010) Effect of ultra son ic2a ssisted extraction on polysacchar ide structure from C oprinus com a tus character ized by FT IR and AFM L IU Na2n 1, ZHANG J ing 2, L IDai 1, XU Ping2p ing 1, WANG Bo 1, SUN Run2guang 1 (1. Laboratory of Biophysics and B iomedical Technology, College of Physics and Information Technology, Shaanxi Normal University, Xiπan , China; 2. College of Food Engineering and Nutrition Science, Shaanxi Normal University, Xiπan , China) Abstract: Polysaccharides were extracted from Coprinus com a tus by boiling water p rocess and ultrasonic2 assisted p rocess, the obtained polysaccharides were designated aswcp and UCP, respectively. The total sugars of WCP and UCP were % and % determ ined by phenol2sulfuric acid m ethod. The crude polysaccharides were purified by CTAB and Sephadex G2200 in sequence, and the obtained ones were designated aswcp321 and UCP321. FTIR showed that there existed 2pyranoside in the polysaccha2 rides and AFM showed thatwcp321 on m ica existed in sp iral and spherical form, but UCP321 existed in spherical form. The ultrasonic collap se of cavitation bubbles generated intense shock waves that damaged the intrachain or interchain hydrogen bonds. Key words: Coprinus com a tus; ultrasonic extraction; polysaccharide : : ( ) ; ( SJ08A16) : (1985 ),,,, : ; ( ),,, E2mail: sunrun2 guang@ snnu. edu. cn

2 1 : 57 (Coprinus com atus),, [ 1-2 ], 3 16, [ 3 ],, [ 4 ],,,,, ( FTIR ) (AFM ) 2, 2, ; H 2 SO 4 NaOH (CTAB ) NaCl, 1. 2 KQ3200 ( ), JY92 ), HPX ( 9162MBE ( ), CF16RX ( ), TU 1810 ( ), EQU INX55 ( B rucher ), A lphal 4 ( Christ ), SPM 9500J3 ( ) : 4 80% (, ) (20 m in 3 ) m in ( 10 s s, 300 W ), 3 ( r/m in 10 m in ) 3 d (UCP) [ 5 ] : 4 80% ( 20 m in 3 ) h, 3 ( r/m in 10 m in ) 3 d (WCP) [ 6 ] WCP UCP, WCP : nm, A 199 = 11747; UCP : nm, A 205 = 11922; 2, 260 nm 280 nm, [ 5 ], WCP UCP [ 7 ] ) mg, 100 ml,, 012 mg/ml 2) 5 g,, 100 ml, 3) ml, 6 50 ml ( 1 0 ml, ), mg/ml ml 014 ml, H 2 SO 4 2 ml, 30 m in, 488 nm (A 488 ) ( ), (A 488 ),

3 58 8 A 488 = R 2 = WCP UCP g, 50 ml, 1 mg/ml 1 ml 25 ml 0104 mg/ml 012 ml , WCP3 UCP3, 1, WCP UCP % %, UCP WCP,, WCP UCP, 1) 5 g WCP UCP 40 ml, 5% (CTAB ) 50 ml,, 12 h r/m in 4 20 m in 10% NaCl, 3 95%,, r/m in 20 m in 3 d ( ),, WCP1 ( g) UCP1 (01597 g) 2) CTAB 1% H 3 BO 3 ph 610, 2 mol/l NaOH ph 1110,, r/m in 4 20 m in, 10% NaCl, 2% ph 710, 3 95%,, 3 d, UCP2 (01335 g) WCP 3), ph 414, 3 95%, 4 24 h, r/m in 4 20 m in,, 3 d, WCP3 (21029 g) UCP3 (21007 g) WCP3 UCP3 WCP3 UCP3 Sephadex G 200 WCP3 1 UCP3 1,, WCP3 UCP WCP3 1 UCP3 1 WCP3 1 UCP3 1, KB r,,, cm - 1 WCP3 1 UCP3 1 : cm cm - 1 O H, ; cm - 1 C H, ; [ 8 ], cm - 1 N H, CH 3 CONH I, ; cm cm - 1 C H, ; [ 9 ], cm - 1 ; [ ], 930 cm - 1, 840 cm - 1,, WCP3 1 UCP3 1 UCP cm - 1 C O, WCP3 1, UCP3 WCP3 1 C O, C 1 C O : O C O, 2. 4 WCP3 1 UCP3 1 WCP3 1 UCP g/ml, 015 L,, 20 m in SPM 9500J3

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