(H ipp op hae rham noid es L. ) , ; SHB 2g , 1. 0, 2. 0, 3. 0, 4. 0, 5. 0 ml mm. : Y = X - 0.
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1 34 10 () V o l. 34 N o Jour. of N o rthw est Sci2T ech U niv. of gri. and Fo r. (N aṫ Sci. Ed. ) O cṫ 2006 Ξ 1, 2, 1, 2, 3, 2, 2 (1, ; 2, ; 3, ) [ ],,, : V ( ) V ( 95% ) = 3 7, (m gv ) 1 10, m in;, [ ] ; ; ; ; [] S [] [ ] (2006) (H ipp op hae rham noid es L. ), (E laegnaceae) (H ip2 p op hae L. ), 8 [1 ], [2 ],, m g g,, [3 ],,, [428 ], [9 ],, , 60 1 mm 1. 2 U 270, eackm an ; ( kh z), o le2parm er ; R 201L, ; SH 2g, ; 200S, Sarto riu s (1), (2) (, ), m g, 100 ml, 20 ΛggmL 0, 1. 0, 2. 0, 3. 0, 4. 0, 5. 0 ml 10 ml, 0. 1 mo lgl l l3 1 ml,,, 10 m in, 430 nm (X ), (Y ), : Y = X , r= Ξ [ ] [ ] (1979- ),,,, [ ] (1963- ),,,, E2m ail: m ym 63@ sina. com
2 10 : ml, 1 ml 10 ml, l2 l3,, 10 m in,, 430 nm, ,, 95% 3 ( 1 1), 1 8, m in, (1) 95% V ( ) V ( 95% ) 0 1, 1 9, 3 7, 1 1, 7 3, 9 1, 1 8, m in, (2) (m gv ) V ( ) V ( 95% ) = 3 7, 1 4, 1 6, 1 8, 1 10, 1 12, m in, ( 3) V ( ) V ( 95% ) 3 7, 1 10, 10, 15, 20, 25, 30 m in, 40, (4) V ( ) V ( 95% ) 3 7, 1 10, 30, 35, 40, 45, m in, ,,,, L 9 (3 4 ) 3 (2 ) , 95%, 95% 1 T able 1 Effect of different so lutions on content of to tal flavono ids m g g So lution ontnet of to tal flavono ids So lution ontnet of to tal flavono ids M ethano l M ethano l and ethyl acetate % 95% ethano l % E thyl acetate and 95% ethano l E thyl acetate % M ethano l and 95% ethano l % 1
3 186 () 34 1,,, V ( ) V ( 95% ) = 3 7,, 95% (m gv ) 2, 1 10,, , 30 m in, m in ,, 50, L evel 2. 2 L 9 (3 4 ) V ( ) V ( 95% ), L 9 (3 4 ) L 9 (3 4 ) T able 2 Facto r Standard list of the o rthogonal experim ental of L 9 (3 4 ) g(m V ) Facto r gm in tim e , , V ( 3 L 9 (3 4 ) T able 3 O rthogonal experim ental L 9 (3 4 ) and the results g ) V (95% ) 3 7, 1 10, m in N o. V ( ) V ( 95% ) g(m V ) gm in tim e g g(m g g - 1 ) To tal flavono ids content (1) 1 4 (1) 10 (1) 30 (1) (1) 1 8 (2) 20 (2) 40 (2) (1) 1 10 (3) 30 (3) 50 (3) (2) 1 4 (1) 20 (2) 50 (3) (2) 1 8 (2) 30 (3) 30 (1) (2) 1 10 (3) 10 (1) 40 (2) 8. 21
4 10 : 3 ontinued of T able N o. V ( ) V ( 95% ) g(m V ) gm in tim e g g(m g g - 1 ) To tal flavono ids content (3) 1 4 (1) 30 (3) 40 (2) (3) 1 8 (2) 10 (1) 50 (3) (3) 1 10 (3) 20 (2) 30 (1) k k k R , ( ) ; ( ) 95% ( ), ; (), : > > > 4 T able 4 V ariation analysis of content of to tal flavono ids Facto r eviation sum of squares egree of freedom M ean square F F value P P value Significant exam ination < < < < E rro r : 3. ; 3 3. N o te 3 is significant difference; 3 3 is greatly significant difference. 2. 4, ; V ( ) V ( 95% ) 3 7, 1 10, 75 2 h 3,,,, 5 5 T able 5 omparison of content of to tal flavono ids betw een ultrasonic and refluxing m g g Extraction frequency Refluxing Extraction frequency Refluxing To tal , 2 RJ m g g 2 3 (1), : V ( ) V (95% ) = 3 7, 1 10, m in m g g (2),, 2 3,,,,,
5 188 () 34 [ 1 ]. [J ]., 1998 (3) : 27. [ ] [ 2 ],. [J ]., 2003, 20 (3) : [ 3 ],,. [J ]., 2003, 6 (6) : 529. [ 4 ],,. [J ]., 2004, 29 (5) : [ 5 ],,. [J ]., 2001, 26 (1) : [ 6 ],,,. [J ]., 2004, 31 (1) : [ 7 ],,. [J ]., 2004, 25 (6) : [ 8 ],,. [J ]., 2003, 6 (6) : 123. [ 9 ],,. [J ]., 2004, 16 (4) : R esearch on u ltrason ic w ave ex traction of to tal flavono ids from fru it m arc of H ipp op hae rham noid es L. J ING Shao- juan 1, 2,M Yang-m in 1, 2, KONG ong-n ing 3, SH I Qing-hua 2, FU J ian-x i 2 (1 olleg e of hem istry & hem ical E ng ineering, S haanx i U niversity of S cience & T echnology, X iany ang, S haanx i , h ina; 2 olleg e of L if e S cience, N orthw est & F U niversity, Y ang ling, S haanx i , h ina; 3 L iaoning ongning P harm aceutical omp any,l td., Fux in,l iaoning , hina) bstract: T he u ltrason ic ex traction of to tal flanovono ids in the fru it m arc of H ipp op hae rham noid es L. w as studied. T he ex traction p rocess of to tal flanovono ids w as op tim ized w ith o rthogonal experim en t, and there w as a comparison betw een the u ltrason ic m ethod and the reflux ing m ethod. T he resu lts indicated that op tim um ex traction condition s w ere determ ined as fo llow s: the m ix tu re so lu tion of V (ethyl acetate) and (3 7) as ex tracting so lven t, stock ratio 1 10 (m ass vo lum e), temperatu re 50, tim e 30 m in. nd the u ltrason ic m ethod w as superio r to the reflux ing techno logy. Key words: u ltrason ic w ave ex traction; fru it m arc of H ipp op hae rham noid es L. ; to tal flanovono ids; ex2 traction techno logy; o rthogonal experim en t ( 183 ) bstract I: (2006) R esearch of all2so lid com b ination ph sen so r and its app lication YNG a i-q in 1, U ao-zhong 2,L I X iang-yang 2, GUO ong 2, XUE L i 2 (1 olleg e of hem istry & hem ical E ng ineering, S haanx i U niversity of S cience & T echnology, X iany ang, S haanx i , h ina; 2 ep artm ent of pp licated hem istry, X iπan U niversity of T echnology, X iπan, S haanx i , h ina) bstract: T h is paper repo rts abou t a new type all2so lid com b ination ph sen so r determ in ing directly ph value of su rface and in side of so lid, sem i2so lid and pasty m ass firstly in ch ina. It also describes in detail the techno logy of p reparing, p roperties of ph determ in ing condition, and effects of o ther sub stanceṡ T he sen so r has low er inner resistance, rap id respon se, w ide m easu re range (ph 1-13), h igh m easu re p recision and good repeat, and its respon se slope is ( ) mv gph, temperatu re coefficien t is mv g. T he all2so lid com b ination ph sen so r no t on ly has the sam e effect as glass electrode, bu t also can be u sed in clin2 ic test, food p rocess, b iochem ical engineering, environm en t test, leather, paper m ak ing, so il su rvey, cu ltu ral relic p ro tection and so on. trode Key words: ph sen so r; in2situ and in tact determ in ing; iridium electrode; g2 g l bare reference elec2
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