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Identification of genes activated and biological markers involved in lysophosphatidic acid (LPA)-induced breast cancer metastasis through its receptor LPA1 Debashish Sahay To cite

fr/tel-01148200 Submitted on 4 May 2015 HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published

1 Identification of genes activated and biological markers involved in lysophosphatidic acid (LPA)-induced breast cancer metastasis through its receptor LPA1 Debashish Sahay To cite this version: Debashish Sahay. Identification of genes activated and biological markers involved in lysophosphatidic acid (LPA)-induced breast cancer metastasis through its receptor LPA1. Cancer. Université Claude Bernard - Lyon I, English. <NNT : 2015LYO10012>. <tel > HAL Id: tel Submitted on 4 May 2015 HAL is a multi-disciplinary open access archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d enseignement et de recherche français ou étrangers, des laboratoires publics ou privés.

11 ALP Alkaline Phosphatase MAG Monoacylglycerol AP-1 Activator Protein 1 MAPK Mitogen Activated Protein Kinase ATX Autotaxin M-CSF Macrophage Colony Stimulating Factor BALF Bronchoalveolar Lavage Fluid MCP-1 Monocyte Chemoattractant Protein 1 BMP Bone Morphogenetic Protein MEC Matrice Extracellulaire BMU Basic Multicellular Unit MMTV Mouse Mammary Tumor Virus BP Bisphosphonate MPP Matrix Metalloproteinase BSP Bone Sialoprotein Msx2 Msh Homeobox CDDP Cisplatin NF-B Nuclear Factor Kappa B CCL-2 Chemokine Ligand 2 NFATc1 Nuclear Factor of Activated T-cell CFU Colony Forming Unit NT-ATX Non-Tumoral Autotaxin COX-2 Cyclooxygenase-2 NUC Nucléase CTGF Connective Tissue Growth Factor OPG Osteoprotegerin CXCR-4 Chemokine C-X-C Receptor 4 OPN Osteopontin DAG Diacylglycerol PA Phosphatidic Acid DKK-1 Dickkopf-related Protein 1 PAP Prostatic Acid Phosphatase Dlx5 Distal-less Homeobox 5 PC Phosphatidylcholin Edg Endothelial Differenciation Gene PDGF Platelet Derived Growth Factor EGF Epidermal Growth Factor PGE2 Prostaglandine E2 ET-1 Endothelin 1 PI3K Phosphoinositide 3-Kinase FAK Focal Adhesion Kinase PKC Protein Kinase C FGF Fibroblast Growth Factor PLA1/2 Phospholipase A1/2 GPCR G Protein-Coupled Receptor PLC Phospholipase C IGF Insulin-like Growth Factor PSA Prostate-Specific Antigen IL Interleukin PTH Parathyroid Hormone IP3 Inositol-triphosphate PTHrP Parathyroid Hormone-related Protein LDL Low Density Lipoprotein RANK Receptor Activator of NF-B LCAT Lecithin Cholesterol Acyl RANK-L Receptor Activator of NF-B Ligand Transferase LIF Leukemia Inhibitory Factor Runx2 Runt Related Transcription Factor 2 LPA Lysophosphatidic Acid S1P Sphingosine-1-Phosphate LPAAT LPA-Acyltransferase SMD Somatomedine-B Domaine LPC Lysophosphatidylcholin TGF Transforming Growth Factor- LPE Lysophosphatidylethanolamine TNF Tumor Necrosis Factor LPP Lipid Phosphate Phosphatase upa Urokinase-Type Plasminogen Activator LPS Lysophosphatidylserine UUO Obstruction Ureteral Unilateral LysoPLD Lysophospholipase D VCAM-1 Vascular Cell Adhesion Molecule 1

34 Lpar1

50 μ μ

64 Affimetrix Fluorescent values Systematic name _at _at _at _at _at _at _at _at _at PC3 MDA-MB-231 MCF _at _at Gene name LPAR1 LPAR2 LPAR3 LPAR4 LPAR5 LPAR6 Edg2/LPA 1 Edg4/LPA 2 Edg7/LPA 3 GPR23/LPA 4 P2Y9 GPR92/LPA 5 P2Y5/LPA 6 PC3 MDA-MB /- +/- +/- +/ MCF /- + Gene symbol PC3 MDA-231 MCF-7 Systematic name Color scale Fold increase

65 β β β β

83 μ μ μμ μ μ

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85 LPA receptor/l32 (Rel. Unit) μ

86 μ μ

87 μ μ

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