Comparison analysis of common volatile constituents in herbal pair radix saposhnikoviae-rhizoma seu radix notopterygii and its single herbs
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- ŌÁĒ Ωκεανός Κορωναίος
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1 41 2 ( ) Vol.41 No Journal of Central South University (Science and Technology) Apr ( ), % 87.35% 86.76% / O657 A (2010) Comparison analysis of common volatile constituents in herbal pair radix saposhnikoviae-rhizoma seu radix notopterygii and its single herbs CHEN Shuai-hua 1, YU Lian-fang 2, LI Xiao-ru 2, LIANG Yi-zeng 2 (1. School of Chemistry and Chemical Engineering, Jiangxi Science and Technology Normal University, Nanchang , China; 2. School of Chemistry and Chemical Engineering, Central South University, Changsha , China) Abstract: Comparison analysis of common volatile constituents in herbal pair (HP) radix saposhnikoviae (RS)-rhizoma seu radix notopterygii (RSRN) and its single herbs was performed using two-dimensional gas chromatography-mass spectrometry (GC-MS) data coupled with alternative moving window factor analysis (AMWFA). In total, 79, 70 and 74 volatile chemical components in volatile oil of RS, RSRN and HP RS-RSRN were separately determined qualitatively and quantitatively, accounting for 85.81%, 87.35% and 86.76% of total contents of volatile oil in RS, RSRN and HP RS-RSRN respectively. The results show that there are 44 common volatile constituents in HP RS-RSRN and single herb RS, and 53 common volatile constituents in HP RS-RSRN and single herb RSRN. The experimental results also show that the volatile chemical components in HP RS-RSRN are mainly from single herb RSRN. Key words: radix saposhnikoviae; rhizoma seu radix notopterygii; herbal pair; gas chromatography-mass spectrometry; alternative moving window factor analysis [1] (RS) (RSRN) ( ) (1963 ) xrli@mail.csu.edu.cn
2 2 441 [2 3] GC-MS (AMWFA) [4 7] [8 10] AMWFA 2 2 [4 7] QP2010 C 8 ~C 20 ( Fluka Chemika ) g [11] 100 g 1.3 OV-1( 30 m 0.25 mm) 50 2 /min /min min He 1.0 ml/min EI 70 ev kv 20~ /s 2 min 1.4 Matlab 6.1 NIST AMWFA 1 GC-MS (TIC) TIC ~ min X TIC ~ min Y 2 X Y 2 X Y 2 AMWFA 3 2 AMWFA 1 X Y ( Y ) ( 3) 4(a) 2 4(b) 3 1 (13~38 88~ ~144) NIST Furan 2-pentyl- Octanal beta-myrcene 99.47% 94.83% 95.21% X Y 5 X Y Vanden [12] R I =100n+100[(t x t n )/(t n+1 t n )] t x n n+1
3 442 ( ) 41 (a) (b) (c) 1 Fig.1 Total ion chromatograms patterns of volatile oils of RS, RSRN and HP RS-RSRN (a) X (b) Y 2 X Y Fig.2 Total ion chromatograms for X peak cluster and Y peak cluster
4 Fig.3 Results of common rank analysis 1 2-pentylfuran; 2 octanal; 3 β-myrcene (a) X (b) Y 5 X Y Fig.5 Resolved chromatograms for peak clusters X and Y (a) (b) 4 Fig.4 Resolution results of common components [13 14] ( ) 85.81% 87.35% 86.76% (14.85%) (10.78%) D- (5.53%) (4.98%) (S) ( )- (4.86%) α- (20.93%) D- (9.57%) α- (8.05%) ( ) (5.74%) γ- (4.83%) (3.28%) - α- (22.32%) α- (8.18%) D- (7.71%) ( ) (5.98%) (3.38%) (2.36%) (1.89%) ( ) ( ) [15 16]
5 444 ( ) 41 1 Table 1 Main chemical components of volatile oils from RS, RSRN and HP RS-RSRN r c /% RS RSRN RS-RSRN R I 1 C 7 H 14 O α- C 10 H C 10 H C 8 H 16 O D- C 10 H γ- C 10 H ( ) C 10 H 18 O α- C 10 H 18 O C 10 H 8 O C 12 H 20 O (S) ( )- C 15 H C 17 H 24 O : r c R I 3 (1) % 87.35% 86.76% (2) (3) 2 [1],,. [M]. :, 1996: 363. XU Qing-hua, LIU Li-hua, ZHAO Rui-hua. Collection of drug pairs in traditional Chinese medicine[m]. Beijing: China Press of Traditional Chinese Medicine, 1996: 363. [2],. [M]. :, 2004: TAN Tong-lai, LIU Qing-ling. Common-used herbal pairs and contraindictions of traditional Chinese medicine[m]. Taiyuan: Shanxi Press of Science and Technology, 2004: [3]. [M]. :, 2000: , TIAN Dai-hua. Practical dictionary of traditional Chinese herbs[m]. Beijing: People s Medical Press, 2000: , [4] ZENG Zhong-da, LIANG Yi-zeng, WANG Ya-li, et al. Alternative moving window factor analysis for comparison analysis between complex chromatographic data[j]. J Chromatogr A, 2006, 1107: [5],,,. [J]., 2006, 27(11): YI Lun-zhao, LIANG Yi-zeng, ZENG Zhong-da, et al. AMWFA method applied to comparative analysis of two-dimensional data with overlapped peaks[j]. Chem J Chin Univ, 2006, 27(11): [6],,,. GC-MS (AMWFA) 3 [J]., 2006, 27(9): YI Lun-zhao, LIANG Yi-zeng, ZENG Zhong-da, et al. Comparative analysis of volatile constituents in Chenpi of different original plants by GC-MS and AMWFA[J]. Chem J Chin Univ, 2006, 27(9): [7],,,. -
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