Supporting Information Figure S1. The functionality of the tagged Arp6 and Swr1 was confirmed by monitoring cell growth and sensitivity to hydeoxyurea (HU). Five-fold serial dilutions of each strain were plated on YPD with or without 50 mm HU and incubated at 30 C or 37 C for 3 days. Figure S2. Localization of Arp6 and Swr1 on chromosome 3. The binding of Arp6-FLAG (top), Swr1-FLAG (middle), and Arp6-FLAG in swr1 cells (bottom) are compared. The position of Tel 3L, Tel 3R, CEN3, and the RP gene are shown under the panels. Figure S3. Localization of Arp6 and Swr1 on chromosome 4. The binding of Arp6-FLAG (top), Swr1-FLAG (middle), and Arp6-FLAG in swr1 cells (bottom) in the whole chromosome region are compared. The position of Tel 4L, Tel 4R, CEN4, SWR1, and RP genes are shown under the panels. Figure S4. Localization of Arp6 and Swr1 on the region including the SWR1 gene of chromosome 4. The binding of Arp6- FLAG (top), Swr1-FLAG (middle), and Arp6-FLAG in swr1 cells (bottom) are compared. The position and orientation of the SWR1 gene is shown. Figure S5. Localization of Arp6 and Swr1 on chromosome 5. The binding of Arp6-FLAG (top), Swr1-FLAG (middle), and Arp6-FLAG in swr1 cells (bottom) are compared. The position of Tel 5L, Tel 5R, CEN5, and the RP genes are shown under the panels. Figure S6. Preferential localization of Arp6 and Swr1 in the 5 end of genes. Vertical bars represent the binding ratio of proteins in each locus. The binding of Arp6-Flag (Top), Swr1-Flag (middle), and Arp6-Flag in swr1 cells (bottom) in the region 228K-244K of Chr 6R were compared. The orientation of transcription of the genes of Watson strand and Crick strand in the region was shown by arrows in the map over the panels. Regions of divergent promoters are indicated with gray shadow. Figure S7. Correlation of the localizations of Arp6 and Swr1. The Arp6-binding log2 ratios of Arp6-binding loci (change p- value <0.025) in wild-type (A) and in swr1 cells (B) are represented as scatterplots versus the Swr1 binding log2 ratio in each Arp6 binding locus of wild-type cells. The yellow lines represent the hypothetical pattern of the data if Arp6 and Swr1 bind equally on the chromosomes. Figure S8. ChIP analysis for Htz1 in cells lacking Arp6 or Swr1. Htz1 association to the promoter of GAL1, SWR1, and ribosomal protein (RPL13A and RPS16B) genes was analyzed using ChIP with an anti-htz1 antibody (abcam, ab4626) and quantified using real-time quantitative PCR in wild-type (WT), arp6, and swr1 cells. The values are indicated as percentage of input DNA obtained by ChIP with anti-htz1 antibody. The data points represent the mean ± SD for at least three independent experiments. Figure S9. Quantitative analysis of RDS1 (YCR106W) and UBX3 (YDL091C) in arp6- and htz1-deletion mutants. The same amount of total RNA from wild-type, arp6, and htz1 cells was analyzed using real-time quantitative RT PCR. The ACT1 gene was analyzed as a control. The relative amount of the transcript of the genes to ACT1 is shown. The data points represent the mean ± SD for at least three independent experiments. Figure S10. ChIP analysis for nuclear pore complex with GAL1 gene in arp6 cells. The association of GAL1 gene with NPC was analyzed using ChIP with an antibody against nuclear pore complex proteins (Mab414, abcam, ab24609) in wild-type (WT) and arp6 cells grown on the glucose- or galactose containing media. Immunoprecipitated DNA was quantified using real-time PCR probed for GAL1 gene. The percentage of recovered DNA over input is plotted relative to wild-type cells on glucose as 1. The data points represent the mean ± SD for at least three independent experiments.
Table S1. Presence of Arp6 in nonrepetitive 10 kb subtelomere zones. (0.05 MB DOC) Table S2. Microarray analysis in arp6δ and swr1δ cells. (1.10 MB XLS) Table S3. Binding of Arp6 and Swr1 on ribosomal protein genes. (0.04 MB DOC) Table S4. Expression of RP genes in arp6δ and swr1δ cells. (0.04 MB XLS) Table S5. Genes markedly down-regulated in arp6 cells. (0.09 MB DOC) Table S6. Strains used in this study. (0.06 MB DOC) Text S1. Primer sequences. (0.04 MB DOC)
SWR1 Arp6 Swr1 Arp6 in swr1 Yoshida et al., Supplementary Fig. S3B
Arp6 Swr1 Arp6 in swr1 Yoshida et al., Supplementary Fig. S5
A 4 Arp6 (log2) 3.5 3 2.5 2 r =0.278, n=2001 1.5 1 0.5 0-2 -1 0 1 2 3 4 5 6 7 Swr1 (log2) B 4 Arp6 in swr1 (log2) 3.5 3 2.5 2 1.5 r =0.138, n=1463 1 0.5 0-2 -1 0 1 2 3 4 5 6 7 Swr1 (log2) Yoshida et al., Supplementary Fig. S6
ChIP analysis for Htz1 in arp6 and swr1 mutants % precipitated by anti-htz1 / input 5 4 3 2 1 WT arp6 swr1 0 GAL1 SWR1 RPL13A RPS16B Yoshida et al. Supplementary Fig. S7
Quantitative rtpcr for Arp6/Swr1-bound genes (x10-3 ) Relative expression to ACT1 8 6 4 2 WT arp6 htz1 0 RDS1 UBX3 Yoshida et al., Supplementary Fig. S8
ChIP for GAL1-nuclear pore association 2.0 WT arp6 p<0.05 % (IP/ input) / WT gluc 1.5 1.0 0.5 0 glucose galactose Yoshida et al., Supplementary Fig. S9
Supplementary Table S1. Presence of Arp6 in nonrepetitive 10 kb subtelomere zones Chr3L (32) 1) Chr3R (34) Chr4L (4) Chr4R (13) Chr5L (15) Chr5R (13) Chr6R (34) Total (145) SWR1 Arp6 binding loci coincidence with Swr1 binding 1 2) (3%) 3) 3 (9%) 0 (0%) 3 (9%) 1 (25%) 1 (8%) 0 (0%) 0 (0%) 3 (20%) 0 (0%) 2 (13%) 0 (0%) 7 (21%) 16 (11%) 2 (6%) 7 (5%) swr1 Arp6 binding loci 3 (9%) 4 (12%) 0 (0%) 5 (38%) 0 (0%) 1 (8%) 9 (26%) 22 (15%) 1) number of subtelomeric probes in the array, 2) number of probes on which the ChIP-chip signal is significantly positive, 3) percentage of binding-positive probes
SupplemantaTable S2 arp6 log2 ratio arp6 t-test p-value swr1 log2 ratio swr1 t-test p-value YAL001C -0.48778725 0.055121132-0.651383868 0.009641293 TFC3 transcription factor tau (TFIIIC) subunit 138 YAL002W -0.50137568 0.02012999 0.465583742 0.134286021 VPS8 Vps8p is a membrane-associated hydrophilic protein which contains a C-terminal cysteine-rich region that conforms to the H2 variant of the RING finger Zn2+ binding motif. YAL003W 0.291356151 0.57828274 0.018063402 0.741564133 EFB1 Translation elongation factor EF-1beta, GDP/GTP exchange factor for Tef1p/Tef2p YAL004W -1.37471567 0.023223502 0.446599197 0.094749311 strong similarity to A.klebsiana glutamate dehydrogenase YAL007C 0.514959137 0.367907397 0.193498974 0.126415188 ERP2 p24 protein involved in membrane trafficking YAL008W -1.07248083 0.003902375 0.04877654 0.757249306 FUN14 hypothetical protein YAL009W -0.83886578 0.039587079-0.007318718 0.898104961 SPO7 sporulation protein YAL010C 0.350119155 0.079737228-0.110610526 0.32107038 MDM10 Mitochondrial outer membrane protein involved in mitochondrial morphology and inheritance YAL011W -0.34259393 0.310843521-0.299325162 0.15316135 possible mitochondrial transit peptide/weak similarity to Mus musculus p53-associated cellular protein YAL012W 1.573242516 0.034945842 0.519382719 0.240287741 CYS3 cystathionine gamma-lyase YAL013W 0.10593589 0.260657892 0.288869805 0.318643395 DEP1 regulation of phospholipid metabolism YAL014C 0.190903734 0.569458913 0.169774313 0.044229011 hypothetical protein YAL015C 0.370541536 0.031276471-0.245836227 0.004561835 NTG1 DNA glycosylase YAL016W -0.64975335 0.0560383 0.036635636 0.638828131 TPD3 protein phosphatase 2A regulatory subunit A YAL017W -1.25829846 0.004518805 0.126513184 0.38324032 FUN31 Serine/threonine kinase YAL018C -0.51051588 0.164932688-0.264509297 0.317542105 3 transmembrane domains/weak similarity to hypothetical proteins YOL047c and YOL048c YAL019W 0.320792247 0.444493655-0.211820784 0.280255331 FUN30 SNF2 protein family YAL021C -0.1074359 0.778044634-0.008790493 0.798167731 CCR4 95 kda containng leucine rich tandem repeats YAL022C 0.656687352 0.001364239 0.428924658 0.115624767 FUN26 predicted membrane protein YAL023C 0.778388229 0.117515196 0.53048886 0.106271155 PMT2 dolichyl phosphate-d-mannose:protein O-D-mannosyltransferase YAL024C 0.327596538 0.491315967 0.065792226 0.797949412 LTE1 putative GTP-exchange protein YAL025C 1.742728628 0.005475432-0.503643316 0.197141618 MAK16 putative nuclear protein YAL026C -0.15225171 0.144316487 0.062776186 0.624950436 DRS2 Membrane-spanning Ca-ATPase (P-type),member of the cation transport (E1-E2) ATPases YAL027W -0.19310171 0.23325453-0.364863449 0.173323366 hypothetical protein YAL028W -1.05821689 0.028312621-0.12586505 0.263146998 similarity to hypothetical protein YOR324c YAL029C 1.414871688 0.169872272 0.17340552 0.14232505 MYO4 myosin YAL030W -0.82724505 0.037223546 0.300700811 0.135945922 SNC1 homolog of Snc2p, vesicle-associated membrane protein (synaptobrevin) homolog, forms a complex with Snc2p and Sec9p YAL031C -0.66093199 0.001932142 0.091597547 0.555114187 FUN21 FUN21 YAL032C -0.45984855 0.100362467 0.119635039 0.26845368 PRP45 pre-mrna splicing factor YAL033W 0.516623095 0.085119298-0.332318037 0.10772256 POP5 An integral subunit of RNase P and apparent subunit of RNase MRP YAL034C -1.37859175 0.009174568 0.462426647 0.033887391 FUN19 Function unknown now YAL034W-A 0.046056852 0.601311241-0.173771005 0.501575118 MTW1 determining metaphase spindle length YAL035C-A 0.455896607 0.408337787 0.428415177 0.170295679 YAL035W 0.75438664 0.04599226-0.221359802 0.054514118 FUN12 97 kda protein YAL036C 1.239106861 0.048698109 0.063806118 0.17119679 FUN11 similar to Xenopus GTP-binding protein DRG YAL037W -0.57645571 0.009922233-0.165687374 0.532682496 strong similarity to GTP-binding proteins YAL038W 0.891293531 0.223803982 0.838341102 0.122662277 CDC19 Pyruvate kinase YAL039C 0.367285033 0.377663351 0.193111406 0.48023897 CYC3 cytochrome c heme lyase (CCHL) YAL040C -0.19482503 0.251202381 0.567158599 0.138150953 CLN3 G(sub)1 cyclin YAL041W 0.121678523 0.623119848 0.001064527 0.995446938 CDC24 Guanine nucleotide exchange factor (a.k.a. GDP-release factor) for cdc42 YAL042W 0.520759379 0.112442757 0.121088992 0.106200684 ERV46 ER-Golgi transport vesicle protein YAL043C -0.09940515 0.785189261 0.642208033 0.063435676 PTA1 pre-trna processing YAL043C-A 0.364729879 0.471390403 0.118430232 0.175428015 YAL044C 1.22688802 0.213909953 0.359553331 0.125672906 GCV3 H-protein subunit of the glycine cleavage system YAL045C -0.15640282 0.262645106 0.601851976 0.070209367 hypothetical protein YAL047C -0.19449606 0.6792255-0.488173264 0.134349146 SPC72 component of spindle pole bodies YAL048C -0.53508764 0.050309883 0.083250034 0.159663585 PEP4 vacuolar aspartic proteasse YAL049C -1.58912253 0.000295413 0.155651056 0.32049334 weak similarity to Legionella small basic protein sbpa YAL053W 1.167219198 0.23272621 0.494264836 0.182351238 strong similarity to hypothetical proteins YOR365c,YGL139w,YPL221w YAL054C -1.09564458 0.024545903 0.245199899 0.445281937 ACS1 inducible acetyl-coenzyme A synthetase YAL055W -1.18899461 0.004853529-0.410131441 0.074131314 PEX22 peroxisomal matrix protein import YAL058C-A 0.154682303 0.736803287 0.434889949 0.03830118 YAL058W -0.3166379 0.01219009 0.124089103 0.085005861 CNE1 Calnexin and calreticulin homolog YAL059W 1.189087482 0.105757458-0.368382138 0.180043241 ECM1 putative transmembrane domain protein involved in cell wall biogenesis YAL060W -0.33257326 0.461367286 0.208542234 0.398809867 FUN49 stereospecific (2R, 3R)-2,3-butanediol dehydrogenase YAL061W -1.7629215 0.014135456 0.322907683 0.261242406 FUN50 similarity to alcohol/sorbitol dehydrogenase YAL062W -1.71445039 0.006829855 0.056824429 0.427171159 GDH3 NADP-linked glutamate dehydrogenase YAL064C-A -0.1786571 0.740798376 0.233634589 0.382026263 strong similarity to Flo1p, Flo5p - putative pseudogene YAL064W -0.74505863 0.145378609 0.286819838 0.453965863 hypothetical protein YAL065C -1.17547087 0.003827097 0.399871097 0.081840231 strong similarity to Flo1p and Flo9p - putative pseudogene YAL066W -0.93408152 0.296857989-0.005238039 0.9931692 weak similarity to membrane protein yybf - Bacillus subtilis YAL067C -1.2512459 0.039595582-0.066498241 0.541023311 SEO1 putative permease/suppressor of Sulfoxyde Ethionine resistance YAL069W -0.14723693 0.771200929 0.404281405 0.099211952 YAR002C-A 0.8089258 0.349749522 0.258873046 0.277899274 ERP1 p24 protein involved in membrane trafficking YAR002W 1.036070284 0.005377987-0.125110616 0.316508824 NUP60 nuclear pore protein YAR003W -0.21157782 0.286119875-0.439692436 0.028854891 FUN16 beta transducin domain/similarity to human RB protein binding protein YAR007C 0.63060681 0.128060752 0.193886043 0.236505966 RFA1 69 kda subunit of the heterotrimeric RPA (RF-A) single-stranded DNA binding protein, binds URS1 and CAR1 YAR008W 0.531614843 0.249599706 0.007320805 0.966485591 SEN34 34kDa subunit of the tetrameric trna splicing endonuclease YAR009C 1.568985076 0.048845245 0.323389091 0.027024819 TY1B Ty1B protein YAR010C 1.440334364 0.067113503 0.4556637 0.009967175 TY1A TY1A protein YAR014C 0.68765935 0.06094559-0.116161101 0.246966 BUD14 maximal growth/similarity to hypothetical protein S.pombe YAR015W 1.028246324 0.28042917-0.063672942 0.498936214 ADE1 phosphoribosyl amino imidazolesuccinocarbozamide synthetase YAR018C 0.244945801 0.50654314 0.208883698 0.000210337 KIN3 protein kinase YAR019C -0.17792809 0.554620603 0.344762613 0.202010355 CDC15 protein kinase domain YAR020C 0.150018392 0.566814473 0.644597596 0.018234699 PAU7 similar to Pau3, member of Pau1 family YAR023C -0.63998255 0.061165085 0.011521673 0.926460784 membrane protein YAR027W -0.69710112 0.111520721-0.196597495 0.124441656 FUN55 membrane protein/strong similarity to YAR028w, YCR007c, YGL053w, YAR031w, FUN59p and YGL051w YAR028W 0.02438456 0.965511157 0.018670564 0.788749248 membrane protein/strong similarity to Fun55p, YGL053w, YCR007c, YAR031w, Fun59p and YGL051w YAR030C 0.304108258 0.377811936 0.037110257 0.897034717 hypothetical protein YAR031W -0.6089193 0.023134268-0.059812785 0.68063699 PRM9 membrane protein/strong similarity to YGL053w, Fun59p, YGL051w, Fun55p, YAR028w and YCR007c YAR033W -0.52963824 0.157687557-0.030386547 0.852864032 FUN59 membrane protein/strong similarity to YGL051w, YGL053w, YAR031w, Fun55p, YAR028w and YCR007c YAR035W -0.07180285 0.912504663-0.088423459 0.289350854 YAT1 Outer carnitine acetyltransferase, mitochondrial YAR042W -0.03006143 0.924880125 0.283718288 0.419166192 SWH1 ankyrin repeat YAR044W -0.00263468 0.994132468-0.054374301 0.653312417 OSH1 Shows homology to the human oxysterol binding protein (OSBP) YAR047C -0.86387318 0.264807528-0.457303432 0.435180037 predicted nuclear targeting signal YAR053W -0.58801994 0.172781392 0.483606121 0.307259816 predicted membrane protein YAR060C 0.132593072 0.858684956 0.077482878 0.674930742 strong similarity to hypothetical protein YHR212c YAR061W -0.49217382 0.264261253-7.98871E-05 0.99978933 strong similarity to N-terminus of Flo1p - putative pseudogene
YAR062W -0.16768339 0.455152005 0.565555252 0.070699921 strong similarity to Flo1p - putative pseudogene YAR064W 0.075841311 0.848648394 0.192771016 0.405714066 Potential membrane protein YAR066W 0.700203408 0.213023476 0.412287539 0.060145965 identical to YHR214w hypothetical protein, similarity to Sta1p YAR068W 0.245010922 0.505026924 0.479488019 0.125500624 Potential membrane protein YAR069C -0.74696325 0.059178657 1.062230426 0.074823538 Potential membrane protein YAR070C -0.05544974 0.578769239-0.202657858 0.753174965 potential mitochondrial transit peptide YAR071W -0.24993142 0.657840395 0.242156128 0.04763318 PHO11 Acid phosphatase, secreted YAR073W 0.808656429 0.19847315-0.391364075 0.012098201 IMD1 IMP dehydrogenase homolog YAR075W 1.350080559 0.042146299 0.285653905 0.110292263 strong similarity to IMP dehydrogenases YBL001C 0.521372342 0.063150214 0.377806625 0.007995235 ECM15 involved in cell wall biogenesis YBL002W 0.644046534 0.11247777 0.436611469 0.050795936 HTB2 Histone H2B (HTB1 and HTB2 code for nearly identical proteins) YBL005W-A 0.711706069 0.066631051 0.397034913 0.029499345 TyA gag protein. Gag processing produces capsid proteins. YBL005W-B 0.806694821 0.004270475 0.44184349 0.099669502 The TyB Gag-Pol protein. Gag processing produces capsid proteins. Pol is cleaved to produce protease, reverse transcriptase, and integrase activities. YBL007C -1.05475926 0.011207344 0.585806022 0.060636472 SLA1 contains 3 SH3 domains, interacts with Bee1p YBL008W -0.25713498 0.395843766-0.291904995 0.082416055 HIR1 putative repressor protein homologous to yeast Tup1p and mammalian retinal transducin ; contains nuclear targeting signal YBL009W 0.377903649 0.410199557-0.139775549 0.535123179 strong similarity to DNA damage responsive Alk1p YBL011W 0.732695584 0.029991524 0.08310294 0.483247349 SCT1 suppressor of choline-transport mutants YBL012C 0.122002089 0.818084302 0.501228611 0.245672062 questionable ORF YBL013W -0.54551921 0.247361356-0.131201515 0.688835462 FMT1 Methionyl-tRNA Transformylase YBL014C 0.145227982 0.439522054-0.259788052 0.108339886 RRN6 member of yeast Pol I core factor (CF) also composed of Rrn11p, Rrn7p and TATA-binding protein YBL015W -1.88887999 0.011757083-0.089791178 0.380359889 ACH1 acetyl CoA hydrolase YBL016W 0.405385742 0.154177413 0.28688141 0.20783228 FUS3 cdc2+ /CDC28 related kinase with positive role in conjugation YBL017C -0.35057353 0.009578128 0.117031097 0.552092446 PEP1 carboxypeptidase Y sorting receptor in late Golgi ; Type I integral membrane protein 166aa cytoplasmic tail, 1300 aa lumenal domain YBL018C 0.429413309 0.189625608 0.017990194 0.520462645 POP8 integral subunit of RNase P and apparent subunit of RNase MRP YBL019W -0.44360937 0.193112906 0.251108094 0.139099165 APN2 AP endonuclease YBL020W -0.14644714 0.399902983 0.129851451 0.139006802 RFT1 67 kda integral membrane protein YBL021C 0.418111438 0.094468832 0.244357052 0.122508233 HAP3 transcriptional activator protein of CYC1 YBL022C 0.057851963 0.823243187-0.158622217 0.282644513 PIM1 mitochondrial ATP-dependent protease YBL023C 0.311056122 0.164045195-0.186899983 0.230537931 MCM2 Minichromosome maintenance protein, transcription factor YBL024W 0.768176955 0.114518321-0.234637929 0.036028563 NCL1 Probable proliferating-cell nucleolar antigen (human p120) YBL025W 0.214419035 0.022039086 0.005455163 0.978683975 RRN10 Upstream activation factor subunit YBL026W 0.550467742 0.123592519-0.418656457 0.061304339 LSM2 snrna-associated protein of the Sm class YBL027W 1.076896847 0.044605028-0.188299044 0.495992786 RPL19B Ribosomal protein L19B (YL14) (L23B) (rpl5l) YBL028C 1.508809573 0.098454761-0.774528492 0.132994667 hypothetical protein - involved in mating-type regulation YBL029W -0.69897514 0.012282849-0.263262192 0.089300671 hypothetical protein YBL030C -0.9969331 0.023634487 0.172120597 0.083005311 PET9 mitochondrial ADP /ATP translocator YBL031W 0.234498822 0.377257191-0.008179032 0.96811451 SHE1 hypothetical protein YBL032W -0.38895763 0.045985811 0.017670569 0.957997296 weak similarity to hnrnp complex protein homolog YBR233w YBL033C 0.267094143 0.071552505-0.124879611 0.137726678 RIB1 GTP cyclohydrolase II YBL034C 0.923515409 0.026769582-0.020444035 0.486715894 STU1 component of the mitotic spindle YBL035C 0.054515907 0.832182456 0.220668279 0.098781864 POL12 B subunit of DNA polymerase alpha-primase complex YBL036C 0.203816669 0.10506225-0.458967973 0.030825796 Homolog to twitching motility protein (P. aeroginosa) YBL037W -0.50995488 0.039507578-0.139849898 0.027220315 APL3 Large subunit of clathrin associated protein complex YBL038W 0.011903164 0.969512114-0.436315028 0.061699688 MRPL16 Mitochondrial ribosomal protein MRPL16 YBL039C 1.641103523 0.101240934 0.074950286 0.541091171 URA7 CTP synthase, highly homologus to URA8 CTP synthase YBL040C 0.414488628 0.337366554 0.416949682 0.003866974 ERD2 encodes the HDEL receptor required for retention of ER proteins YBL041W 0.302973703 0.334706978-0.259201017 0.10000259 PRE7 proteasome subunit YBL042C 1.549013444 0.235516871 0.314682648 0.192857668 FUI1 uridine permease YBL043W 0.858385132 0.0428885-0.778409799 0.03665693 ECM13 (putative) involved in cell wall biogenesis YBL044W -0.19569315 0.623648411-0.729946359 0.191344608 hypothetical protein YBL045C -0.79287106 0.006255025 0.176388947 0.299817881 COR1 44 kda core protein of yeast coenzyme QH2 cytochrome c reductase YBL046W 0.3859612 0.081664134-0.350887344 0.132967365 weak similarity to hypothetical protein YOR054c YBL048W -1.69306352 0.001064383 0.178308538 0.672634552 hypothetical protein YBL049W -1.69519851 0.003376388-0.008092027 0.984683049 strong similarity to hypothetical protein - human YBL050W -0.41391761 0.141981425-0.196815486 0.078672316 SEC17 peripheral membrane protein required for vesicular transport between ER and Golgi YBL051C 0.401509441 0.181824349 0.222847746 4.56E-05 hypothetical protein YBL052C 0.26976272 0.09049595-0.752252813 0.012898381 SAS3 involved in silencing at HMR YBL053W 0.044255282 0.911991193 0.139375926 0.707387167 questionable ORF YBL054W 1.307919818 0.023417029 0.204899594 0.153605143 Homolog to myb transforming proteins YBL055C 0.358491013 0.481932574-0.250402414 0.070487082 similarity to hypothetical S.pombe protein YBL056W -0.2865873 0.173469012-0.273953725 0.042656459 PTC3 protein phosphatase type 2C YBL057C -0.32078047 0.406630018-0.046334252 0.444338998 strong similarity to hypothetical S.pombe protein YBL058W -0.37962486 0.217951768-0.078208021 0.418770366 SHP1 putative regulatory subunit for Glc7p, a phosphatase required for glucose repression YBL059W 0.462211321 0.198916479-0.10554177 0.37878554 weak similarity to hypothetical protein YER093c-a YBL060W 0.077003492 0.582507009-0.474268578 0.026406853 has homology to the sec7 domain of gtp exchange factors YBL061C 0.648278479 0.033692682 0.000146021 0.999335964 SKT5 Probable Ca++ binding membrane protein (prenylated) YBL062W 0.579637219 0.033550656-0.196912671 0.008308333 questionable ORF YBL063W 0.525577525 0.275713837 0.208608817 0.330500908 KIP1 kinesin related protein YBL064C -1.18003348 0.008713809-0.0127579 0.959222788 similar to thiol-specific antioxidant enzymes such as rehydrin /peroxiredoxin YBL065W -0.8494896 0.108915753-0.191671497 0.466324124 questionable ORF YBL066C -0.69422618 0.143848615 0.262468137 0.257366393 SEF1 putative transcription factor YBL067C -0.51185761 0.052322307-0.525739488 0.149092917 UBP13 ubiquitin carboxyl-terminal hydrolase YBL068W 0.552260638 0.019179853 0.013744302 0.745293033 PRS4 ribose-phosphate pyrophosphokinase 4 YBL069W -0.00725388 0.987732728-0.025129386 0.8304204 AST1 involved in targeting of plasma membrane [H+]ATPase YBL070C 0.478395103 0.258147059 1.449527927 0.218831982 questionable ORF YBL071C 1.665104942 0.002268259 0.397246315 0.005194289 hypothetical protein YBL072C 2.202569138 0.067628298 0.170422683 0.317507363 RPS8A Ribosomal protein S8A (S14A) (rp19) (YS9) YBL073W -0.40522369 0.129399014-0.621780705 0.1180431 questionable ORF YBL074C -0.5145695 0.044240228-0.299340283 0.200705068 AAR2 MATa1-mRNA splicing factor YBL075C -1.40272499 0.038021942 0.103764729 0.516248929 SSA3 heat-inducible cytosolic member of the 70 kda heat shock protein family YBL076C 0.581969244 0.096179447 0.035319811 0.6166625 ILS1 cytoplasmic isoleucyl-trna synthetase YBL077W 1.172703984 0.079621289 0.370471985 0.179126374 questionable ORF YBL078C -0.66417108 0.112692286 0.015724015 0.901271398 AUT7 Aut7p has homology to LC3, a microtubule-associated protein from rat. YBL079W -0.00134744 0.994454663 0.242089261 0.035448994 NUP170 Nucleoporin highly similar to Nup157p and to mammalian Nup155p (nup170 mutant can be complemented with NUP155) YBL080C -0.13981846 0.418501084-0.465852982 0.073379563 PET112 62-kDa protein YBL081W 1.017113724 0.195899025 0.379908274 0.083287683 hypothetical protein YBL082C 0.289125972 0.451855036 0.462326619 0.194832428 RHK1 putative Dol-P-Man dependent alpha(1-3) mannosyltransferase involved in the biosynthesis of the lipid-linked oligosaccharide YBL083C 0.619332739 0.241584502 0.780879169 0.044070098 questionable ORF YBL085W 0.836764694 0.157704611 0.163004677 0.490774252 BOI1 BEM1-binding protein YBL086C -0.01015504 0.966715371-0.043958057 0.723896157 involved in sugar metabolism YBL087C 1.69104705 0.026184735 0.387822345 0.047703769 RPL23A Ribosomal protein L23A (L17aA) (YL32) YBL089W 0.155452074 0.725891376 0.094285963 0.732266472 similar to amino acid transport proteins
YBL090W 0.130930356 0.447528626-0.444727802 0.137346139 MRP21 Component of the small subunit of mitochondrial ribosomes YBL091C -0.01592092 0.928530418 0.071111829 0.023003347 MAP2 methionine aminopeptidase 2 YBL092W 1.359675889 0.089999806 0.242349467 0.22278823 RPL32 Ribosomal protein L32 YBL093C 1.630542104 0.066445879-0.40984133 0.216685648 ROX3 RNA polymerase II holoenzyme /mediator subunit YBL094C 0.152904229 0.515609558 0.109011177 0.180970885 questionable ORF YBL095W -0.59080451 0.004927264-0.029633732 0.812978335 similarity to C.albicans hypothetical protein YBL096C 0.809338829 0.02935606 0.23293904 0.416154081 questionable ORF YBL097W 0.770433894 0.07368835-0.361063246 0.146007606 BRN1 involved in chromosome maintenance ; similar to Drosophila barren, Xenopus XCAP-H, and human BRRN1 YBL098W 0.097303301 0.636822178-0.297569623 0.074939331 similar to kynurenine 3-monoxygenase YBL099W -1.74485012 0.000944171 0.178292671 0.304697955 ATP1 mitochondrial F1F0-ATPase alpha subunit YBL100C -0.95817031 0.163043291-0.229257504 0.056340994 YBL101C -1.01526877 0.019962145 0.067425577 0.379628367 ECM21 involved in cell wall biogenesis YBL101W-A -0.00154932 0.996505124 0.057147373 0.689363261 TyA gag protein. Gag processing produces capsid proteins. YBL102W -0.08028531 0.556077806 0.31092902 0.159417155 SFT2 similar to mammalian syntaxin 5 YBL103C 0.932300032 0.088718445-0.176095807 0.262861795 RTG3 Probable cytochrome c subunit, copper binding YBL104C -0.2971584 0.219170985 0.019758268 0.843113947 weak similarity to S.pombe hypothetical protein SPAC12G12.01c YBL105C -0.29337151 0.173608517 0.261086456 0.115317532 PKC1 Protein Kinase C YBL106C -0.47851114 0.139929171 0.226125663 0.146577753 SRO77 yeast homolog of the Drosphila tumor suppressor, lethal giant larvae YBL107C 0.200780264 0.388844229-0.749110279 0.052248549 hypothetical protein YBL111C -1.05003272 0.021440559 0.440875337 0.111353535 strong similarity to subtelomeric encoded proteins YBL112C 1.191795333 0.432220643 0.287071313 0.006904427 strong similarity to subtelomeric encoded proteins YBR001C -0.86549996 0.021060784-0.160508473 0.39524395 NTH2 Neutral trehalase, highly homologous to Nth1p YBR002C 0.730283602 0.033873306-0.289764979 0.119604952 RER2 cis-prenyltransferase YBR003W 0.100169714 0.480672151-0.063057253 0.495326415 COQ1 hexaprenyl pyrophosphate synthetase YBR004C 1.295560514 0.221698384 0.132653291 0.387742124 similarity to S.pombe hypothetical protein SPAC18B11.05 YBR005W 0.924653953 0.249522237 0.104687752 0.366762602 strong similarity to hypothetical protein YDR003w YBR006W -1.52560357 0.008957363-0.085669344 0.671433134 UGA2 succinate semialdehyde dehydrogenase YBR007C 0.517934495 0.057829417-0.066320668 0.644967763 hypothetical protein YBR008C -0.37376243 0.011740901 0.30804565 0.089231413 FLR1 Major Facilitator Transporter YBR009C 1.79607489 0.160621987-0.128225562 0.64736466 HHF1 Histone H4 (HHF1 and HHF2 code for identical proteins) YBR010W 0.269847874 0.520269233-0.267808917 0.167146345 HHT1 Histone H3 (HHT1 and HHT2 code for identical proteins) YBR011C 0.711606067 0.094200466 0.487560056 0.077906597 IPP1 Inorganic pyrophosphatase YBR012C 0.020050112 0.961740639-0.446376426 0.056950447 hypothetical protein YBR012W-A 0.92915986 0.054663881 0.458739681 0.025930922 TyA gag protein. Gag processing produces capsid proteins. YBR012W-B 0.773827916 0.0389811 0.425029049 0.021804999 The TyB Gag-Pol protein. Gag processing produces capsid proteins. Pol is cleaved to produce protease, reverse transcriptase, and integrase activities. YBR013C -0.1495441 0.5715657-0.891616784 0.176515109 hypothetical protein YBR014C -0.41076534 0.117959158-0.328502192 0.223802248 Glutaredoxin homolog YBR015C -0.29682379 0.107936446 0.82261182 0.018058492 MNN2 putative Golgi alpha-1,2-mannosyltransferase YBR016W -0.25382865 0.022234531 0.611211515 0.056973834 strong similarity to hypothetical proteins YDL012c and YDR210w YBR017C 0.664689855 0.106855369 0.255720225 0.063517032 KAP104 karyopherin beta 2, yeast transportin YBR018C -1.06276079 0.032440167 0.012997681 0.872951126 GAL7 galactose-1-phosphate uridyl transferase YBR019C -0.12604533 0.238774221 0.347151947 0.127300079 GAL10 UDP-glucose 4-epimerase YBR020W -0.55474059 0.016210963-0.158015082 0.298445027 GAL1 galactokinase YBR021W 0.954354328 0.01878998 0.205890789 0.238395816 FUR4 uracil permease YBR022W 0.171306102 0.537296624-0.349686836 0.001841431 hypothetical protein YBR023C 0.912263581 0.103935193 0.098668748 0.380427345 CHS3 chitin synthase 3 YBR024W -0.37180761 0.185577626-0.224130893 0.118990855 SCO2 SCO1 protein homolog (S. cerevisiae) YBR025C -0.03253562 0.761316582-0.334788143 0.025259256 probable purine nucleotide-binding protein YBR026C -1.79666811 0.001139509-0.48834428 0.011001479 MRF1' Nuclear protein that binds to T-rich strand of core consensus sequence of autonomously replicating sequence YBR027C -0.31314044 0.383916149 0.438248599 0.092445593 hypothetical protein YBR028C -0.38795783 0.072758896-0.181174175 0.043927954 Probable ser /thr-specific protein kinase, homolog to YKR2 and YPK1 (S. cerevisiae) YBR029C 1.873221503 0.202566076 0.439750334 0.025890012 CDS1 CDP-diacylglycerol synthase, CTP-phosphatidic acid cytidylyltransferase, CDP-diglyceride synthetase YBR030W 0.316712433 0.44526422 0.074472979 0.614228624 involved in inositol biosynthesis YBR031W 1.613544394 0.07541802 0.301061615 0.252079647 RPL4A Ribosomal protein L4A (L2A) (rp2) (YL2) YBR032W 1.575502204 0.339951907 0.793243286 0.144359497 hypothetical protein YBR033W -0.6757668 0.124799999 0.098550683 0.308753005 Probable regulatory Zn-finger protein, / homolog to YKL251 / YBR034C 2.228738544 0.203125817-0.196495292 0.02866966 HMT1 nuclear protein arginine methyltransferase (mono- and asymmetrically dimethylating enzyme) YBR035C 0.130349129 0.80921713 0.13172004 0.328784715 PDX3 pyridoxine (pyridoxiamine) phosphate oxidase YBR036C 0.117417048 0.74068934 0.410956178 0.19827052 CSG2 contains 9 or 10 putative membrane spanning regions ; putative Ca2+ binding protein (homology to EF-hand Ca2+ binding site) YBR037C -0.17067536 0.570646079 0.105247342 0.414885441 SCO1 inner mitochondrial membrane protein YBR038W 0.305385214 0.644042442 0.282925941 0.398128117 CHS2 chitin synthase 2 YBR039W -0.59427459 0.08086054 0.001575296 0.966726017 ATP3 gamma subunit of mitochondrial ATP synthase YBR040W -0.00766125 0.989131704 0.778830158 0.523131592 FIG1 integral membrane protein YBR041W 0.436472937 0.314141745 0.289904233 0.04546478 FAT1 Fatty acid transporter YBR042C 0.242945218 0.666002818-0.20606029 0.171424177 Probable membrane-bound small GTPase YBR043C 0.953943167 0.427049582-0.075521683 0.353173974 similarity to benomyl/methotrexate resistance protein YBR044C 0.355155265 0.565508787 0.088443323 0.520230538 TCM62 mitochondrial protein ; (putative) chaperone YBR045C -0.58375387 0.209083688-0.179532154 0.320474084 GIP1 putative Glc7 regulatory subunit YBR046C -0.50900369 0.078687096-0.268805608 0.196696574 ZTA1 Homolog to quinone oxidoreductase (E. coli) YBR047W 0.345131914 0.214329255-0.375710016 0.119849878 hypothetical protein YBR048W 0.603507542 0.135601762 0.102493926 0.627849268 RPS11B Ribosomal protein S11B (S18B) (rp41b) (YS12) YBR050C -2.74260779 0.001202836-0.011903495 0.793037867 REG2 putative Glc7 regulatory subunit YBR051W -0.79954413 0.198635591 0.200747252 0.555528587 questionable ORF YBR052C -0.8837639 0.008813195 0.174626596 0.274162583 Homolog to YCR004, obr1 (S. pombe), trp repressor binding protein (E. coli) YBR053C -1.26196201 0.007530385 0.064568231 0.696539186 YBR054W -0.82416682 0.202367524 0.058253171 0.867115473 YRO2 Homolog to HSP30 heat shock protein YRO1 (S. cerevisiae) 7 YBR055C 0.065904703 0.47461113-0.202555733 0.139190571 PRP6 RNA splicing factor YBR056W -1.83846967 0.000290729-0.210984021 0.039238575 Homolog to glucan-1,3--glucosidase (EC 3.2.1.5 ; S. cerevisiae) 2 YBR057C 0.158454264 0.490280912-0.566428757 0.108714877 MUM2 similar to ubiquitin C-terminal hydrolase, involved in meiosis YBR058C -0.19594084 0.322758995-0.164023692 0.137950714 UBP14 Ubiquitin-specific protease YBR058C-A 0.558714653 0.129197159 0.109070903 0.250594504 TSC3 involved in sphingolipid biosynthesis YBR059C -0.34663956 0.363023316-0.050829423 0.24066309 AKL1 Serine-threonine protein kinase YBR060C 0.27971224 0.531653043-0.045186231 0.808705202 ORC2 origin recognition complex subunit 2 YBR061C 0.761585229 0.024965194 0.018200302 0.846360181 Homolog to ftsj protein (E. coli), / YCR054 / YBR062C 0.288290044 0.014632732 0.143601011 0.300327054 similarity to rat neurodegeneration associated protein 1 YBR063C -0.5003856 0.056147129-0.656806607 0.011283221 Probable phosphopanthethein-binding protein YBR064W -0.34570159 0.402576203 0.046060395 0.886920932 questionable ORF YBR065C -0.2940729 0.363688044-0.545170337 0.083682507 ECM2 (putative) involved in cell wall biogenesis and mrna splicing YBR066C -0.40136934 0.47744334-0.259366352 0.013534042 NRG2 homologue of NRG1 YBR067C 2.657290624 0.081659989 0.72893684 0.159134158 TIP1 cell wall mannoprotein YBR068C 1.13550092 0.1369263 0.318765583 0.082272981 BAP2 probable amino acid permease for leucine, valine, and isoleucine YBR069C 1.41986915 0.028245424 0.191598764 0.181834414 TAT1 Amino acid transport protein for valine, leucine, isoleucine, and tyrosine
YBR070C 0.287654792 0.261069689 0.488579697 0.050932508 involved in osmotolerance YBR071W -0.23404102 0.135316418 0.14837919 0.017435005 hypothetical protein YBR072W -3.85355268 4.35429E-05 0.055242838 0.878228723 HSP26 heat shock protein 26 YBR073W 0.608580829 0.001122145-0.12753796 0.410414866 RDH54 Putative helicase similar to RAD54 YBR074W -0.19530328 0.290335452 0.128192761 0.57522542 Homolog to aminopeptidase Y (S. cerevisiae) YBR075W -0.25958784 0.091043279 0.413375993 0.045078881 hypothetical protein YBR076W 1.684282025 0.193100244 0.559383528 0.162138918 ECM8 involved in cell wall biogenesis YBR077C 0.234624033 0.520606837 0.083520781 0.08062932 hypothetical protein YBR078W 0.634290106 0.458226343 0.744162625 0.020510107 ECM33 Homolog to sporulation specific protein SPS2 (S. cerevisiae) YBR079C 0.483314438 0.091929106-0.020022852 0.790565166 RPG1 translation initiation factor eif3 YBR080C 0.27960895 0.261880228 0.054506911 0.51489527 SEC18 cytoplasmic protein involved in protein transport between ER and Golgi ; ATPase YBR081C 0.015336017 0.901637858-0.602169656 0.084527129 SPT7 transcription factor, member of the histone acetyltransferase SAGA complex YBR082C 0.745786076 0.215734436 0.458275415 0.052156525 UBC4 ubiquitin-conjugating enzyme YBR083W 0.377419467 0.234500318 0.371096396 0.091487714 TEC1 transcription factor of the TEA /ATTS DNA-binding domain family, regulator of Ty1 expression YBR084C-A 0.770050182 0.112341057-0.104795619 0.566028018 RPL19A Ribosomal protein L19A (L23A) (rpl5l) (YL14) YBR084W 1.188056859 0.069026391-0.132718809 0.233223694 MIS1 mitochondrial C1-tetrahydroflate synthase YBR085C-A -0.45963288 0.173741308-0.115548995 0.121630525 YBR085W 0.775536388 0.315388654-0.006882046 0.9720062 AAC3 mitochondrial ADP /ATP translocator YBR086C -0.51962399 0.013227985 0.514072473 0.011704358 IST2 Probable transmembrane protein YBR087W 0.366791886 0.080158132-0.203183639 0.274914747 RFC5 Subunit 5 of Replication Factor C ; homologous to human RFC 38 kda subunit YBR088C 0.582217388 0.430217799 0.172054271 0.147877261 POL30 Proliferating cell nuclear antigen YBR089C-A 0.353168257 0.001712314-0.052165544 0.367424144 NHP6B 11-kDa nonhistone chromosomal protein YBR089W 0.502895686 0.47092082-0.00422055 0.987306874 questionable ORF YBR090C-A 0.234674927 0.291935548-0.135266362 0.047404854 NHP6B 11-kDa nonhistone chromosomal protein YBR091C 0.504693529 0.216331994 0.315297708 0.017157673 MRS5 Nuclear protein involved in mitochondrial intron splicing YBR092C 1.736442403 0.129025706 0.595934021 0.093824035 PHO3 Acid phosphatase, constitutive YBR093C 0.04984957 0.938038614 0.35220872 0.085780489 PHO5 Acid phosphatase, repressible YBR094W -0.42722508 0.162996742-0.214104274 0.107077494 weak similarity to pig tubulin-tyrosine ligase YBR095C 0.009708822 0.962635018-0.725473168 0.050087248 hypothetical protein YBR096W 1.074963895 0.37420027-0.152282676 0.231555453 hypothetical protein YBR097W -0.114237 0.747320952-0.03755605 0.341800838 VPS15 Myristoylated Serine /threonine protein kinase involved in vacuolar protein sorting YBR098W -0.01240989 0.97371254-0.471351921 0.010062509 MMS4 putative transcriptional (co)activator for DNA damage YBR099C 0.168035608 0.814245141 0.466871476 0.069777313 weak similarity to T.brucei mitochondrion hypothetical protein 6 YBR100W 0.008731516 0.990177079 0.31732812 0.40871522 questionable ORF YBR101C 0.248460016 0.413730422-0.142347219 0.261543905 weak similarity to S.pombe hypothetical protein SPBC3B9.01 YBR102C -0.17083911 0.592619313-0.456775537 0.075861196 EXO84 Component of the exocyst complex ; homolog in rat brain called rexo84. YBR103W -0.08552846 0.629108182-0.268252215 0.055159072 SIF2 535 amino acid protein containing 4 WD-40 repeats and a nuclear localization signal YBR104W 1.624948092 0.23686685-0.305829446 0.036009706 YMC2 mitochondrial carrier protein YBR105C 2.185107187 0.324790108 0.399023242 0.026388571 VID24 peripheral vesicle membrane protein YBR106W 1.150168552 0.218305029 0.182319988 0.000366591 PHO88 regulator of Pho81, involved in regulating phosphate transport YBR107C -0.13651739 0.434793362 0.178345267 0.261216833 IML3 weakly similar to chitin synthases, involved in chromosomal segregation and mitosis YBR108W -0.4074947 0.226531476 0.555071992 0.093589902 Probable transcription factor YBR109C 0.468904245 0.030916527 0.149840856 0.389029838 CMD1 Calmodulin YBR110W 0.087676938 0.770914879 0.0151659 0.799135669 ALG1 beta-1,4-mannosyltransferase YBR111C -0.58976495 0.009540505-0.238620354 0.037828356 YSA1 Homolog to serendipity protein (D. melanogaster) YBR112C -0.34174347 0.253248329-0.111182611 0.374115035 CYC8 Transcription regulatory protein YBR113W 0.140320837 0.450798336-0.054012496 0.72494431 questionable ORF YBR114W -0.29088133 0.191290459 0.001168098 0.9891889 RAD16 Radiation repair protein, putative DNA helicase YBR115C 0.967191336 0.317466492 0.385197467 0.146944543 LYS2 alpha aminoadipate reductase YBR116C -0.80811305 0.257222452 0.335862557 0.445169198 questionable ORF YBR117C -1.2675551 0.086050055 0.467605524 0.057156045 TKL2 transketolase, homologous to tkl1 YBR118W 0.784392039 0.113379642 0.571117676 0.052583248 TEF2 translational elongation factor EF-1 alpha YBR119W -0.30175105 0.270843113-0.428445728 0.063364151 MUD1 U1 snrnp A protein YBR120C 0.144147453 0.0063578-0.551668384 0.004814249 CBP6 Translational activator of COB mrna YBR121C 0.973757136 0.066296132 0.312584642 0.125733917 GRS1 Glycyl-tRNA synthase YBR122C -0.0868928 0.553959129-0.275073492 0.065433593 MRPL36 Mitochondrial ribosomal protein MRPL36 (YmL36) YBR123C -0.06468012 0.794681223-0.072130675 0.796488601 TFC1 transcription factor tau (TFIIIC) subunit 95 YBR124W 0.147055961 0.696897079 0.360877443 0.21655836 questionable ORF YBR125C -0.10566963 0.455088005 0.132585626 0.350320599 PTC4 Type 2C protein phosphatase YBR126C -1.59513377 0.000235348 0.253060829 0.285855024 TPS1 56 kd synthase subunit of trehalose-6-phosphate synthase /phosphatase complex YBR127C 0.151899799 0.718318147 0.20003993 0.122565984 VMA2 vacuolar ATPase V1 domain subunit B (60 kda) YBR128C -1.26969913 0.005563605-0.054226244 0.560332521 APG14 involved in autophagy YBR129C -0.38589593 0.10676634-0.624126925 0.046736002 OPY1 involved in mating pathway YBR130C 1.038823318 0.108846277-0.199476763 0.375345651 SHE3 involved in cell polarity YBR131W 0.485247561 0.036200754-0.249066232 0.072746414 CCZ1 involved in sporulation, caffeine, calcium, and zinc sensitivity YBR132C -1.20617204 0.008997214 0.500139776 0.143146781 AGP2 Amino acid permease YBR133C -0.00064304 0.996279319 0.369732782 0.150705474 HSL7 regulator of Swe1p kinase YBR134W -0.07990403 0.57447668 0.743239008 0.072579885 questionable ORF YBR135W 0.691560473 0.076914446 0.071993601 0.676545206 CKS1 subunit of the Cdc28 protein kinase YBR137W -0.32311625 0.305348182-0.425401757 0.014491896 hypothetical protein YBR138C 0.136795656 0.4981847-0.039309561 0.874119515 HDR1 High-Dosage Reductional segregation defective YBR139W -0.96543825 0.063276494 0.461400818 0.304871161 Probable serine-type carboxypeptidase (EC 3.4.16.1) YBR141C 0.591206019 0.08365463 0.034631733 0.781829905 hypothetical protein YBR142W 0.924343108 0.07339726-0.580227207 0.057499394 MAK5 Probable pre-mrna splicing RNA-helicase YBR143C 0.90735503 0.133972393-0.319131944 0.046765953 SUP45 Homolog of erf1 (eukaryotic Release Factor 1) in other metazoans. YBR144C 0.787480735 0.017024633 0.111093197 0.707300023 hypothetical protein YBR145W 1.69860482 0.199756232 0.314429383 0.17851089 ADH5 alcohol dehydrogenase isoenzyme V YBR146W 0.852689267 0.044002513-0.427577062 0.005947323 MRPS9 Probable mitochondrial ribosomal protein S9 YBR147W 0.086878594 0.894271649 0.01857904 0.909256222 strong similarity to hypothetical protein YOL092w YBR148W 0.057009993 0.9378736-0.505071738 0.016737578 YSW1 Spore-specific protein YBR149W -0.14603105 0.560710049 0.322233096 0.118067079 ARA1 D-arabinose dehydrogenase YBR150C -0.06849098 0.61877767 0.277404305 0.084780353 TBS1 Probable Zn-finger protein YBR151W 0.140882013 0.673956787 0.048499399 0.637535428 APD1 Actin Patches Distal YBR152W -0.31696976 0.186103218-0.014294108 0.805824901 SPP381 U4 /U6.U5-associated snrnp protein ; contains a PEST proteolysis motif YBR153W 0.880774474 0.037629888-0.137620267 0.430846051 RIB7 Riboflavin biosynthesis protein YBR154C 0.979098423 0.114884829-0.678603044 0.069627695 RPB5 25-kDa RNA polymerase subunit (common to polymerases I, II and III) YBR155W 0.926643818 0.015741964-0.423299063 0.192250705 CNS1 component of Hsp90p chaperone machinery YBR156C 0.621018615 0.041226544-0.030346914 0.793541769 SLI15 Mitotic spindle protein involved in chromosome segregation. YBR157C -1.71986712 0.006172362-0.248741575 0.085629762 ICS2 Increased Copper Sensitivity YBR158W 0.236090001 0.758909223 0.003422235 0.975268559 CST13 Chromosome STability protein YBR159W -0.40798658 0.146111319-0.208230612 0.088504671 similarity to human 17-beta-hydroxysteroid dehydrogenase YBR160W 1.382688997 0.033637934-0.089047975 0.31179147 CDC28 protein kinase catalytic subunit YBR161W -0.08785666 0.596919377 0.10257468 0.214915307 Homolog to suppressor of reduced viability of starvation (SUR1, S. cerevisiae)
YBR162C 2.767805354 0.381081775 1.025781406 0.062845102 TOS1 similarity to hypothetical protein YJL171c YBR162W-A 1.090844591 0.12050417-0.031166834 0.876106659 YSY6 involved in the secretory pathway YBR163W 0.083305197 0.771358216-0.075321539 0.202356609 DEM1 Weak similarity to Pta1p (pre-trna processing protein) YBR164C 0.386971951 0.146788473-0.30730063 0.279077924 ARL1 ADP-ribosylation factor-like protein 1 YBR165W 0.478450374 0.031594004-0.110574637 0.068503035 UBS1 positive regulator of CDC34, involved in ubiquitin-mediated degradation YBR166C 1.077223888 0.119812637-0.348329997 0.052540766 TYR1 Prephenate dehydrogenase (NADP+) YBR167C 0.725530505 0.085284247-0.575616057 0.091172258 POP7 Pop7 protein, an integral subunit of RNase P and apparent subunit of RNase MRP YBR168W -0.33172247 0.013489087-0.238383677 0.214573005 weak similarity to hypothetical protein YLR324w YBR169C -2.00430362 0.001309944-0.237279361 0.207385649 SSE2 HSP70 family member, highly homologous to Sse1p YBR170C -0.38862164 0.009161953-0.121312664 0.233974664 NPL4 Suppressor of SEC63 (S.cerevisiae), novel ER translocation component YBR171W 0.465550263 0.391762515-0.176381153 0.13428994 SEC66 glycoprotein complexed with Sec62p and Sec63p in the Sec63 complex, an integral endoplasmic reticulum membrane protein complex required for translocation of presecretory proteins YBR172C 0.947045973 0.058028345-0.240825609 0.324855802 SMY2 Kinesin-related protein suppressing myosin defects (MYO2) YBR173C 0.543487569 0.028689108 0.89073289 0.059037897 UMP1 20S proteasome maturation factor YBR174C -0.09574649 0.828021009 0.26542884 0.343600031 weak similarity to hypothetical protein YLR324w YBR175W 0.798115784 0.356656435-0.050382926 0.123809345 Probable GTP-binding protein YBR176W 0.068803244 0.470897562-0.394319913 0.027244144 ECM31 Alpha-Ketoisovalerate Hydroxymethyltransferase YBR177C -0.40821177 0.268763947 0.220857848 0.117486244 EHT1 alcohol acyl transferase YBR178W 0.171444756 0.6310156 0.259352923 0.551351751 questionable ORF YBR179C 0.242929175 0.48422383-0.167702237 0.424783503 FZO1 homolog of Drosophila melanogaster fuzzy onions gene ; integral protein of the mitochondrial outer membrane which can be isolated as part of a high molecular weight complex YBR180W 0.497811941 0.321911208-0.383876643 0.054128279 DTR1 dityrosine transporter MFS-MDR YBR181C 1.517480365 0.18681994-0.277696415 0.046526669 RPS6B 40S ribosomal gene product S6B (S10B) (rp9) (YS4) YBR182C 1.07336623 0.32780709 0.107229696 0.503375908 SMP1 Probable DNA-binding transcription factor, Homolog to SRF /SL-2 YBR183W -0.32932225 0.100358245 0.024285921 0.81373346 YPC1 alkaline ceramidase with reverse activity YBR184W 0.532165774 0.05305851-0.540524633 0.016569575 MEL1 alpha-galactosidase YBR185C -0.21994541 0.229580372-0.395388433 0.0119799 MBA1 involved in assembly of mitochondrial respiratory complexes YBR186W 0.490784688 0.38844514 0.192577179 0.445672946 PCH2 Putative ATPase YBR187W 0.690417855 0.049928975-0.257469763 0.087483562 probable membrane protein YBR188C 0.492925293 0.053081384-0.025801954 0.86150773 NTC20 splicing factor YBR189W 0.950615102 0.051056367 0.047228918 0.81597576 RPS9B Ribosomal protein S9B (S13) (rp21) (YS11) YBR190W 0.854062672 0.084939876 0.106675398 0.396889145 questionable ORF YBR191W 0.575714408 0.535778578 0.080457252 0.439970647 RPL21A Ribosomal protein L21A YBR192W 0.509881138 0.005373514-0.202658011 0.151330428 RIM2 Probable carrier protein, mitochondrial YBR193C -0.34403382 0.012995649 0.03899841 0.70048146 MED8 Stoichiometric member of mediator complex YBR194W 0.822733309 0.027278178-0.138921462 0.204751803 hypothetical protein YBR195C 0.225699086 0.306618626 0.036364069 0.407332903 MSI1 p50 subunit of the yeast omatin Assembly Factor-I (CAF-I) negative regulator of ras-mediated camp induction ; homologous to beta subunit of GTP-binding proteins YBR196C 1.367685111 0.219334764 0.633114599 0.120857313 PGI1 Glucose-6-phosphate isomerase YBR197C -0.38684265 0.205152386 0.138519858 0.310187653 YBR198C -0.5545939 0.044090014-0.139263158 0.110161572 TAF90 Probable transcription-associated factor protein, probable -transducin type YBR200W -0.15441417 0.540836335-0.262692526 0.109752107 BEM1 contains two SH3 domains YBR201W 0.056424906 0.888422959-0.235405671 0.187107537 DER1 involved in degradation in the ER YBR203W -0.33775006 0.576361511-0.2045828 0.089979675 hypothetical protein YBR204C -0.90498283 0.00040729-0.360269663 0.102683009 Probable serine-active lipase, peroxisomal (EX 3.1.1.-) YBR205W -0.27879779 0.043595084 0.018995774 0.840490764 KTR3 Putative alpha-1,2-mannosyltransferase YBR206W 0.318754718 0.295731593 0.345147806 0.229701265 questionable ORF YBR207W 0.242939647 0.453039051 0.107702995 0.194904608 FTH1 probable membrane protein YBR208C -0.88846186 0.148064307 0.245161263 0.391157674 DUR1,2 Urea amidolyase (contains urea carboxylase and allophanate hydrolase) YBR209W 2.248661321 0.297551441 0.381258931 0.281716371 hypothetical protein YBR210W 0.940789823 0.054030573 0.313341233 0.206973905 strong similarity to D.melanogaster cornichon protein YBR211C -0.31232008 0.154179443-0.349551777 0.068109672 AME1 regulator of microtubule stability YBR212W -0.16875438 0.27183768 0.70435044 0.055337423 NGR1 negative growth regulatory protein YBR213W 0.273519351 0.582528693 0.202209774 0.245502392 MET8 Effector in the expression of PAPS reductase and sulfite reductase YBR214W -0.57347024 0.126908367-0.125397825 0.063363362 SDS24 nuclear protein similar to pombe sds23 YBR215W 0.017037639 0.945053353-0.372937798 0.19145978 HPC2 highly charged, basic protein YBR216C -0.00884559 0.953941411-0.167736664 0.234462904 strong similarity to hypothetical protein YGL060w YBR217W -0.29756814 0.097019236-0.832730836 0.036759735 APG12 involved in autophagy YBR218C -1.09366079 0.009460379 0.31101953 0.03629335 PYC2 pyruvate carboxylase YBR219C 0.335357966 0.235666601 0.453103199 0.146167607 YBR220C -0.14753261 0.152773414 0.043535552 0.58680398 similarity to human acetyl-coenzyme A transporter YBR221C 0.665723843 0.023979323 0.345653586 0.090351575 PDB1 beta subunit of pyruvate dehydrogenase (E1 beta) YBR222C 0.177197636 0.328760318 0.523832581 0.115135526 FAT2 Probable AMP-binding protein YBR223C -0.36083419 0.162988917 0.236622488 0.091031072 hypothetical protein YBR224W 0.533319791 0.523528918-0.105555358 0.450829681 questionable ORF YBR225W -0.45521538 0.133909158 0.149731073 0.12255293 hypothetical protein YBR226C -0.1493116 0.540340059 0.398830403 0.134948712 questionable ORF YBR227C -0.02690793 0.804430878-0.542974523 0.06158401 MCX1 Mitochondrial ATP-binding protein, similar to ClpX YBR228W -0.15251099 0.540466654-0.30761674 0.088148112 similarity to hypothetical A.thaliana protein YBR229C -0.65280659 0.004625347 0.346345079 0.066488919 ROT2 Glucosidase II YBR230C -1.32094486 0.005924621 0.609470924 0.098929907 hypothetical protein YBR231C -0.19457994 0.205157819-0.12528603 0.492388082 AOR1 Actin Overexpression Resistant YBR232C -0.25897947 0.337666247-0.256882124 0.220949911 questionable ORF YBR233W 0.298632757 0.196470769-0.167429571 0.303627903 PBP2 Homolog to human hnrnp complex K protein YBR234C -0.15061112 0.59134678 0.327554697 0.154113499 ARC40 component of Arp2 /Arp3 protein complex YBR235W -0.51010999 0.136185575 0.324796317 0.268142217 similarity to bumetanide-sensitive Na-K-Cl cotransport protein YBR236C -0.19651971 0.061628045-0.424220761 0.074955691 ABD1 RNA (guanine-7-)methyltransferase (cap methyltransferase) YBR237W -0.04008757 0.626709146-0.669193526 0.026466683 PRP5 RNA helicase homolog YBR238C 1.051169484 0.099958654 0.041128377 0.699872898 strong similarity to general chromatin factor Spt16p YBR239C -0.07996564 0.770885499 0.202238628 0.315917042 Probable Zn-finger protein YBR240C 0.721961605 0.06041823 0.08649645 0.403227564 THI2 Probable Zn-finger protein YBR241C -0.76927116 0.145479129 1.14753597 0.115479885 Probable sugar transport protein YBR242W 0.224206378 0.436104651-0.246551427 0.166744907 Probable ATP /GTP-binding protein YBR243C 0.126453815 0.601538183 0.511759088 0.087117753 ALG7 UDP-N-acetyl-glucosamine-1-P transferase (GPT) YBR244W -0.04024886 0.876819875-0.116780138 0.370779064 GPX2 Probable glutathione peroxidase (EC 1.11.1.9) YBR245C -0.13301951 0.656090366-0.071741569 0.452528359 ISW1 ATPase component of a four subunit chromatin remodeling complex YBR246W 0.388094181 0.115107815 0.389870478 0.015081075 hypothetical protein YBR247C 0.806603288 0.088880609-0.254803962 0.212989418 ENP1 Putative 57 kda protein with an apparent MW of 70 kda by SDS-PAGE YBR248C 0.653441412 0.163592168-0.191623286 0.181164198 HIS7 glutamine amidotransferase:cyclase, also called imidazole glycerol phosphate synthase YBR249C 1.348261644 0.153199225 0.032589822 0.736940757 ARO4 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthase isoenzyme YBR250W 0.224285641 0.140766893 0.073786024 0.397492969 hypothetical protein YBR251W -0.11786867 0.494992133-0.507917338 0.045434132 MRPS5 Probable mitochondrial ribosomal protein S5 YBR252W 1.903287036 0.147563507 0.458420776 0.017844943 DUT1 dutp pyrophosphatase (dutpase) YBR253W -0.14706684 0.324101008-0.219063569 0.388466951 SRB6 transcription factor, part of Srb /Mediator complex