THE EXPRESSION AND SIGNIFICANCE OF11β-HSD 2 AND TNF-α IN PLACENTA WITH HYPERTENSION DISORDERS COMPLICATING PREGNANCY

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1 402 Journal Of Inner Mongolia Medical University Oct.2017 Vol.39 No.5 11β-HSD 2 TNF-α * β 2 11β-Hydroxysteroid dehydrogenase Type 2 11β-HSD 2 -α tumor necrosis factor-alpha TNF-α PCR 11β-HSD 2 TNF-α mrna 1 11β-HSD 2 mrna P< TNF-αmRNA P< β-HSD 2 TNF-α r = P<0.05 TNF-α 11β- HSD 2 11β- 2 11β-HSD 2 -α TNF-α HDCP R717 A DOI: /j.cnki.issn x X THE EXPRESSION AND SIGNIFICANCE OF11β-HSD 2 AND TNF-α IN PLACENTA WITH HYPERTENSION DISORDERS COMPLICATING PREGNANCY ZHOU Xiao-xia LIU Xiao-ying The Affiliated Hospital Inner Mongolia Medical University Hohhot China Abstract Objective To detect the expression and relationship of 11β-HSD 2 and TNF-α in placenta with hypertension disorders complicating pregnancy.in order to explore the effect and significance of the occurrence and progression of this disease. Methods 120 pregnant women were selected including 40 patients of normal pregnancy group 80 cases of hypertensive disorders complicating pregnancy group including 40 patients of preeclampsia in mild group 40 patients of severe preeclampsia group.i took their placental tissue and detected the expression of 11β-HSD 2 and TNF-α in three groups by Real-Time PCR method.the correlation between 11β-HSD 2 and TNF-α was evaluated.re MS liuxiaoyingll@ yahoo.cn

2 sults 1 The expression level of 11β-HSD 2 mrna in hypertensive disorders complicating pregnancy group is significantly lower than that in the normal pregnancy group. And that in severe preeclampsia group is significantly lower than that in preeclampsia mild group P< The expression level of TNF-α mrna in hypertensive disorders complicating pregnancy group is significantly higher than that in the normal pregnancy group.and that in severe preeclampsia group is higher than that in preeclampsia mild group P< The expression levels of 11β-HSD 2 and TNF-α in hypertensive disorders in pregnancy are negative correlation r = P<0.05.Conclusion TNF-α may be involved in the regulation of 11β-HSD 2 and inhibit its activity. They probably interact with each other through some direct or indirect way.they both participate in the development of hypertensive disorders in pregnancy. Key words 11β-HSD 2 TNF-α hypertensive disorders complicating pregnancy Hypertensive disorder complicating pregnancy HDCP 5% ~ 12% ~ HDCP 4.2 /10 40 HDCP % 40 HDCP 3 HDCP Hamad RR HDCP 5 3 HDCP β-HSD 2 RNA glucocorticoid GC RT-PCR GC GC 11β -HSD 2 TNF - α PCR GC 7 Invitrogen SIGMA 3 11β-HSD 2 K15 TU - 11β-HSD TECHNE RT -PCR TNF-α ABI 7500 Fast HDCP TNF-α cm EP 9 HDCP β RT-PCR HSD 2 TNF-α mrna 11β - HSD 2 TNF - αm RNA 1 HDCP RNA RNA

3 404 Journal Of Inner Mongolia Medical University Oct.2017 Vol.39 No RNA cdna 10μL 37 15min 85 5s 2.1 cdna 3 NCBI 3 Genbank NM P> Invitrogen Real - Time PCR GAPDH 11β-HSD 2 Forward primer 5-GGC- CAAGGTTTCCCAGTGA β - HSD 2 Reverse Primer 5 -TTGTGCCAGGAGGGTGTTTG - 3 TNF -α Forward primer 5 -CCCTGCCCCAATCCCTTT- 3 TNF - α Reverse Primer 5 - GCCCCCAAT- TCTCTTTTTGAG-3 GAPDH Forward primer 5 - CCACATCGCTCAGACACCAT- 3 GAPDH Reverse Primer 5 - CGCCCAATACGACCAAATC Fast RT-PCR PCR PCR 20μL 95 30s PCR 95 30s 60 30s s 60 1min 95 15s Real - Real time PCR Time PCR CT 1 PCR C T 2.3 TNF-α PCR TNF-α CT GAPDH Real time PCR CT CT CT CT β-HSD2 TNF-α CT 2 - CT 3 SPSS 16.0 CT β-HSD2 TNF-α mrna P< C T 11β-HSD β -HSD 2 mrna TNF-α P< β CT P< SPSS 16.0 x±s 珋 TNF-α mrna P< P<0.05 TNF-αmRNA TNF-αmRNA mrna P < Spearman P < β-HSD 2 TNF-α 1 Tab.1 3 n x±s 珋 Comparison of age of three groups of subjects n x±s 珋 n ±5.63 x±s 珋 F P ± ± Tab.2 n x±s 珋 Comparison of gestational age of three groups of subjects n x±s 珋 n ±1.74 x±s 珋 F P ± ± β-HSD2 11β-HSD 2 2 HSD2 mrna 11β-HSD 2 mrna

4 Spearman r = P< β-HSD 2 TNF-α 1 11β-HSD 2 Real time PCR GAPDH Fig.1 11β-HSD 2 Real time PCR amplification expression results compared with the internal reference GAPDH Tab TNF-α GAPDH Fig.2 TNF-α expression in the three groups of histograms compared with the ginseng GAPDH 11β-HSD 2 TNF-αmRNA Relative quantitative analysis of11β-hsd 2 and TNF-α mrna expression 11β-HSD 2 TNF-α F / = = 2.17 F / = = 1.90 F / = = F / = = 2.04 F / = = F / = = β-HSD 2 mrna 5 11β-HSD 2 mrna Tab.4 Results of 11β-HSD 2 mrna Tab.5 Analysis of the expression differences of expression in the three groups 11β-HSD 2 mrna in both groups n Chi-Square Hc P Mean Difference P * * * TNF-αmRNA 7 TNF-α mrna Tab.6 Results of TNF-αmRNA Tab.7 Analysis of TNF-α mrna expression expression in the three groups differences in both groups n Chi-Square Hc P Mean Difference P * * * * -1 HDCP HDCP HDCP HDCP 11β-HSD 2 GC 11β

5 406 Journal Of Inner Mongolia Medical University Oct.2017 Vol.39 No.5 -HSD 2 HDCP 10 HDCP AngⅡ 11β-HSD 2 AngⅡ TNF-α TNF-α GC 11 11β-HSD 2 PGI 2 AngⅡ 12 Shang Y 13 11β-HSD 2 TNF-α meishan AngⅡ 11β-HSD 2 11β-HSD 2 Kossintseva 18 11β-HSD 2 HDCP 7 Ca 2+ camp TNF-α 11β-HSD 2 11β-HSD 2 11β-HSD 2 11β-HSD 2 Na + -Ca 2+ Ⅱ angio - tensin Ⅱ AngⅡ AngⅡ I HDCP 14 Ferrari 11β - HSD 2 15 HDCP β-HSD 2 11β-HSD 2 TNF-α Fig.3 Scatter plot of11β-hsd 2 and TNF-α in placenta of hypertensive disorder complicating pregnancy 4 11β-HSD 2 11β-HSD 2 11β -HSD 2 TNF -α HDCP HDCP 11β -HSD 2 mrna TNF-α mrna HDCP HDCP TNF-α TNF-α 11β-HSD 2 TNF-α 11β-HSD 2 mrna 11β-HSD 2 TNF-α TNF-α 11β-HSD 2 HDCP 11β-HSD 2 TNF-α HDCP Lanz Kadereit 17 AngⅡ HDCP 11β-HSD 2 MAPK K AngⅡ AngⅡ Ⅱ2 AT2 HDCP MAPKK 11β-HSD 2 HDCP HDCP

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