Optimization of fermentation process for achieving high product concentration high yield and high productivity

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1 1128 CHEMICAL INDUSTRY AND ENGINEERING PROGRESS ( ) (1) (2) (3) (4) (5) TQ 92 A Optimization of fermentation process for achieving high product concentration high yield and high productivity DU Guocheng LIU Liming LI Yin CHEN Jian (Key Laboratory of Industrial Biotechnology Ministry of Education Sourthern Yangtze University Wuxi Jiangsu China) Abstract In order to achieve high yield high concentration and high productivity of metabolite production a series of optimization strategies for the fermentation process were developed (1) Nutrition and environmental conditions optimization strategy based on microorganism metrology; (2) Environmental conditions shift strategy in batch fermentation based on physiological characteristics; (3) Optimization strategies based on reaction kinetics model; (4) Optimization strategies based on metabolic flux analysis; (5) Optimization strategies based on bio-technology system. These optimization strategies were successfully applied to many metabolites production. Therefore a simplifying quantifying modeling and grading principle for fermentation process optimization was summarized. This principle may provide an approach to enhancing the development of biotechnology and fermentative food. Key words high yield high concentration high productivity fermentation optimization () () [1 2] ( ) E mail jchen@sytu.edu.cn

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4 [21 22] [23] [24 25] [26] 1.4 MFA ( ) ATP NADPH NADH ( 3) [13] ATP NADH ATP [27] L- L NADH FADH [28] ( ) 3 Streptococcus zooepidemicus 1.5 PHAs PHAs PHAs PHAs 4 [29] ATP GSH GSH ATP ATP ATP GSH ADP ATP GSH ATP GSH 6.2 g/l ATP ATP ADP AMP ATP [30 31]

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