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1 West China Journal of Stomatology Vol. 22 No. 6 December 2004 [ ( 2004) ( ; ; ) [ ] H 2 S Halimeter SPSS ; ( ) ; [ ] ; ; ; ; [ ] R [ ] A The Study of the Relationship of Malodor and Microbial Composition of Interdental and Subgingival Plaques in Periodon2 titis Patients LIU Yi 1 HUANG Hai-yun 1 ZHANG Jin-cai 2 WANG Song-ling 3. (1. Key. Laboratory of Oral Biomedical En2 gineering Ministry of Education Sichuan University Chengdu China ; 2. Dept. of Oral Medicine Guangdong Provincial Hospital of Stomatology Guangzhou China ;3. Salivary Gland Disease Center Beijing Hospital for Stomatology Capital University of Medical Sciences Beijing China) [ Abstract] Objective The study is to investigate the microbial composition of interdental and subgingival plaques of periodon2 titis patients with or without malodor to explore the relationships between periodontitis and oral malodor.methods 20 patients of periodontitis with malodor were chosen from 210 patients of periodontitis and the clinical parameter of plaque index ( PLI) gingi2 val bleeding index ( GBI) and probing depth ( PD) were measured and compared with the control group which had periodontal dis2 ease without malodor. During the experiment the interdental and subgingival microbial samples in both groups were collected and sent to anaerobic culture for 48 hrs then the total CFUΠml of each sample were counted and each type of bacteria was separated and identified. All of the data were analyzed by using the statistical software SPSS Results (1) There were no satistical differences on PLI GBI PD between experimental group and control group. (2) The percents of leptospira in both interdental and subgingival plaques of test group were significantly higher than that of the control group ( P < 0. 01). (3) Either the interdental or in subgingival plaques the count results of CFUΠml were similar in both groups ( P > 0. 05). (4) The proportions of malodor pro2 ducing anaerobic bacteria in interdental gingival plaque such as P. gingivalis and Veillonella were singnificantly different be2 tween test group and control group. Conclusion The proportions of VSC s producing anaerobic bacteria in interdental gingival plaque may be play the significant roles in oral malodor. Further studies should be taken to elucidate the relationship between mal2 odor and periodontitis. [ Key words] periodontitis ; oral malodor ; plaque ; bacteria ; anaerobic culture VSCs) 1 2 ; 3 ( volatile sulfur compounds VSCs VSCs [ ; [ ] (1972-) Morita 4 24 mm [ ] Tel :

2 West China Journal of Stomatology Vol. 22 No. 6 December VSCs 5 : 6 : ( Fusobacterium) ( PD > ( Veillonella) ( T. denticola T. d) ( P. gingivalis P. g) ( Bacte2 roides) ( Peptostreptococcus) Fuso2 bacterium T. d P. g Bacteroides : 20 s 1 30 s 1. 1 ; : ; mm ; 4555 : 10 l ; ; - (BHI-S ) h 20 (colony forming unit CFUΠml) 10 : : (BHI) ; 3 (37 48 h) ; (37 48 h) ; Halimeter 150 ppb (Halimeter ) SPSS H 2 S ppb ; t ; 05 (0 1 ; ; 5 ) : ; ; 2 ; 2 h ; PLI GBI 1178 PD 415 mm ; 10 (plaque 6 4 PLI 3179 GBI 1162 PD index PLI) ( gingival bleeding index GBI) (probing depth PD) 7 4 mm) 1 ml ( 0115 g 1 % CaCl ml 100 ml) 4177 mm ( P > 0105)

3 West China Journal of Stomatology Vol. 22 No. 6 December 2004 ( P > 0105) ( P > 0105) ( P < 0101) 2 3 Tab 3 Major bacteria in interdental plaques in the two ( P > 0105) ; ( P groups < 0105) ( n = 10) ( n = 20) ( P < 0101) ( gx s %) 1 P1 P2 ( gx s %) ( gx s %) # Tab 1 The results of microbial composition of interdental plaques in the two groups( gx s %) # P ( gx s %) # Tab 2 The results of microbial composition of subgingival # plaques in the two groups( gx s %) # : P1 3 P < ; P2 # P < P Tab 4 Major bacteria in subgingival plaques in the two groups ( P > 0105) ( P < 0105) ( P < 0105) # # # ( n = 10) ( gx s %) ( n = 20) P1 P2 ( gx s %) # # # ( P > 0105) : P1 3 P < ; P2 # ( P < 0105 P < 0105) Halimeter (CFUΠml) : BANA ( ) lg CFUΠml ( ) lg CFUΠml ; Halimeter 9 ( ) lg CFUΠml (81754 Halimeter H 2 S 01329) lg CFUΠml

4 West China Journal of Stomatology Vol. 22 No. 6 December ( Halimeter) 3. 2 P. g mgl L- G - P. g W83 Rosenberg ; 12 P. g ( 15 ) Parya vi- Gholami VSCs 54 Bosy 16 VSCs Veillonella ( P. oralis) Goldberg Fusobacterium T. d P. g Tiomny 13 Bacteroides Veillonella Peptostreptococcus VSCsQuirynen 17 P. g P. i ( Fusobacte2 rium nucleatum F. n) BHI? Waler 14 6 h VSCs 450 ppb 300 ppb 24 h 6 d VSCs VSCs 300 ppb VSCs 3 BHI 30 min 500 ppb VSCs VSCs ; VSCs VSCs Moriy2 ama 18 : VSCs ; ( P < 0. 05) VSCs ( P > 0105) ; 3 ( P > 0105) 30 ( P < 0105) ; VSCs VSCs

5 West China Journal of Stomatology Vol. 22 No. 6 December 2004 ( P > 0105) VSCs 1993 : ; VSCs 324. VSCs F. n [ ] 1 ] Bosy A Kulkarni GV Rosenberg M et al. Relationship of oral mal2 odor to periodontitis : evidence of independence in discrete subpopula2 tionsj. J Periodontol (1) : ] Yaegaki K Sanada I. Volatile sulfur compounds in mouth air from clinically healthy subjects and patients with periodontal disease J. J Periodontal Res (4) : ] Ratcliff PA Johnson P. The relationship between oral malodor gingivitis and periodontitisj. J Periodontol (5) : ] Morita M Wang HL. Relationship between sulcular sulfide level and oral malodor in subjects with periodontal disease J. J Periodontol (1) : ] Rosenberg M Kozlovsky A Gelernter I et al. Self-estimation of oral malodorj. J Dent Res (10) : ] De Boever EH Loesche WJ. Assessing the contribution of anaerobic microflora of the tongue to oral malodorj. J Am Dent Assoc (10) : ]. M. : 2001 : ]. M. : 9 ] Tonzetich J Kestenbaum RC. Odor production human salivary frac2 tions and plaquej. Arch Oral Biol (6) : ] Rosenberg M Septon I Eli I et al. Halitosis measurement by an in2 dustrial suphide monitorj. J Periodontol (4) : ] Paryavi- Gholami F Minah GE Turng BF. Oral malodor in children and volatile sulfur compound-producing bacteria in saliva : preliminary microbiological investigation J. Pediatr Dent ( 6) : ] Goldberg S Cardash H Browning H et al. Isolation of Enterobacte2 riaceae from the mouth and potential association with malodor J. J Dent Res (11) : ] Tiomny E Arber N Moshkowitz M et al. Halitosis and Helicobacter pylori : a possible linkj?j Clin Gastroenterol (3) : ] Waler SM. On the transformation of sulfur-containing amino acids and peptides to volatile sulfur compounds (VSC) in the human mouthj. Eur J Oral Sci (5 Pt 2) : ]. 79 J (7) : ] Bosy A Kulkarni GV. Relationship of oral malodor to periodontitis : evidence of independence in discrete subpopulations J. J Periodon 2 tol (1) : ] Quirynen M Van Eldere J Pauwels M et al. In vitro volatile sulfur compound production of oral bacteria in different culture media J. Quintessence Int (5) : ] Moriyama T. Clinical study of the correlation between bad breath and subgingival microfloraj. Shikwa Gakuho (9) : () 70 ISSN CN ΠR1993 : Π : 336 ( ) : ; : ; guangzhou. gd. cn

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