Advances of Loop - Mediated Isothermal Amplification

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1 J DH- BV J Bonsor S J Pearson G J. Current clinical applications of photo - activated disinfection in restorative dentistry J. Dent Update J J. 12. AL J J Ⅶ J. 3 Brown SB Brown EA Walker I. The present and future role of photo dynamic therapy in cancer treatment J. Lancet Oncol J J J. 16. J J J Blakeslea trispora H1 - J REVIEW ARTICLES 1 2* loop - mediated isothermal amplification LAMP DNA 2 LAMP LAMP Q78 A doi /j. issn X Advances of Loop - Mediated Isothermal Amplification HUANG Huo - qing 1 YU Ang 2* State Key Laboratory of Marine Environmental Science Environmental Science Research Center Xiamen University Xiamen China Abstract Loop - mediated isothermal amplification LAMP which relys on a DNA polymerase with strand displacement activity and two pairs of specially designed primers is an novel nucleic acid amplification method no repeated temperature cycling no expensive equipment the LAMP reaction was carried out under isothermal conditions with a high efficiency and a high speed it has been widely applied to the detection of such pathogens as bacteria virus parasite etc as well as sexing of animal embryos. The basic principle advantages relatived to other gene amplification techniques the methods to judge target sequence as well as application of LAMP were reviewed finally some existing problems and corresponding measures and prospects for LAMP were discussed in order to provide reference for reader studying. Key words loop - mediated isothermal amplification DNA amplification technique application 0 PCR real - time PCR 1 PCR

2 91 Tsugunori Notomi LAMP 60 ~ 65 PCR 2 LAMP LAMP Fig. 2 The starting structure for LAMP cycling FIP F2c LAMP F1c F1 LAMP F1 F2 1 LAMP - DNA DNA 1. 1 LAMP 1. 3 LAMP LAMP PCR LAMP 1 LAMP LAMP 6 FIP BIP F3 B3 2 1 ~ 10 FIP F1c F2 BIP B1c B2 PCR ' F1c F2c F3c 5' B1 B2 B3 30 ~ 60min Bst DNA P FIP 5' F1c 2 O Mg 2 + = Mg 2 P 2 O 7 dntp P F1 2 O 4-7 FIP F3 BIP B3 DNA 400 nm BIP B2c PCR 5 LAMP PCR 1 1 Table 1 PCR LAMP Characteristics comparison between PCR and LAMP methods LAMP PCR 1 LAMP Fig. 1 The structure of LAMP primers 1. 2 DNA 65 DNA 1. 4 LAMP 4 LAMP FIP F2c DNA F3 F3c DNA n dntp = DNA n + P 2 O LAMP SYBR Green Ⅰ HNB Picogreen SYBR Green Ⅰ HNB 10 hqhuang147@ 163. com * SYBR Green Ⅰ LAMP xmu. edu. cn 10 LAMP

3 92 HNB Leo L. M. Poon SARS RT - LAMP 31 SARS 2 LAMP LAMP minrt - PCR 2 ~ 3 h HAV 2. 1 HAV Ⅲ real - time RT - PCR Yoshiteru Aoi 11 LAMP DNA real - time PCR 65 1 h DNA LAMP LAMP 12 LAMP RNA cfu /g 13 LAMP Ⅰ DNA RNA LAMP RNA PCR LAMP DNA RT - PCR 63 PCR Hiromi Ikadai 29 LAMP 14 resa 30 LAMP LAMP LAMP 2. 2 ~ 3. 4 CFU /100 g 2 d DNA PCR 15 LAMP fg PCR 10 3 Jun - Hu Chen 31 Lateral flow dipstick LFD LAMP min 7. 7 cfu / LAMP 115 ml PCR nested PCR DNA LAMP PCR real - time PCR 2. 4 LAMP Hiroki 16 Hirayama 32 LAMP 2. 2 M. A. Zoheir 33 LAMP DNA EB CuSO 4 45 min Ting Cai 17 LAMP HBV 100% real - time PCR LAMP 3 WSSV 3. 1 VP26 WSSV LAMP pg /μl LAMP 300 bp PCR PCR LAMP LAMP 19 PCR LAMP 45 min LAMP PCR PCR 100 LAMP RNA 64% PCR Tetsuo Yoneyama 21 RT - LAMP HAV 0. 4 ~ 0. 8 FFU /5 μl RT - LAMP H1N1 LAMP LAMP PCR 88. 9% ~ 94. 4% 1 h Khairy PCR

4 93 Bst DNA blue J. Bio Techniques LAMP 10 Njiru Zablon Kithinji Mikosza Andrew Stanislaw John Armstrong Tanya et al. Loop - mediated isothermal amplification LAMP method for rapid detection of Trypanosoma brucei rhodesiense J. Plos Negl Trop Dis e Yoshiteru Aoi Mariko Hosogai Satoshi Tsuneda. Real - time quantitative LAMP loop - mediated isothermal amplification of DNA as a simple PCR HNB method for monitoring ammonia - oxidizing bacteria J. J Biotechnol LAMP J. Hidenori Tani ABC - LAMP LAMP 13. LAMP AB - QProbe J J J LAMP J Ting Cai GuoQiang Luo Jin Yang et al. Development and evaluation LAMP of real - time loop - mediated isothermal amplification for hepatitis B virus DNA quantification A new tool for HBV management J. J Clin Virol LAMP J. 21 Tetsuo Yoneyama Tomoko Kiyohara Noriko Shimasaki et al. Rapid Tsugunori Notomi Hiroto Okayama Harumi Masubuchi et al. Loop - mediated isothermal amplification of DNA J. Nucleic Acids Research J. H1N1 J Kelly A. Curtis Donna L. Rudolph S. Michele Owen. Rapid detection J of HIV - 1 by reverse - transcription loop - mediated isothermal amplification RT - LAMP J. J Virol Methods J J S LAMP J tion of PRRSV by a reverse transcription loop - mediated isothermal amplifi- 8. LAMP cation assay J. Virologica Sinica J Motoki Goto Eiichi Honda Atsuo Ogura et al. Colorimetric detection of loop - mediated isothermal amplification reaction by using hydroxyl anphthol LAMP 18. J J LAMP 20 Leo L. M. Poon Cynthia S. W. Leung Masato Tashiro et al. Rapid detection of the severe acute respiratory syndrome SARS coronavirus by a loop - mediated isothermal amplification assay J. Clin Chem and real - time detection of hepatitis A virus by reverse transcription loop - mediated isothermal amplification assay J. J Virol Methods Yang JinLong Yang Rui Cheng AnChun et al. A simple and rapid 6. method for detection of Goose Parvovirus in the field by loop - mediated isothermal amplification J. Virology Journal Lei Zhang Ye - bing Liu Lei Chen et al. Rapid and sensitive detec- 26. LAMP J

5 94 32 Hiroki Hirayama Soichi Kageyama Satoru Moriyasu et al. Rapid se- 27. xing of bovine preimplantation embryos using loop - mediated isothermal Ⅰ J amplification J. Theriogenology Khairy M. A. Zoheir Ahmed A. Allam. A rapid method for sexing the J bovine embryo J. Anim Reprod Sci Hiromi Ikadai Hiroko Tanaka Nona Shibahara et al. Molecular evidence of infections with Babesia gibsoni parasites in Japan and evaluation of the diagnostic potential of a loop - mediated isothermal amplification method J. J Clin Microbiol LAMP method for specific nucleic acid sequences using competitive polymerase J chain reaction with an alternately binding probe J. Anal Chem Jun - Hu Chen Feng Lu Chae Seung Lim et al. Detection of Plasmodium vivax infection in the Republic of Korea by loop - mediated isothermal amplification LAMP J. Acta Tropica Hidenori Tani Tatsuya Teramura Ken Adachi et al. Technique for quantitative detection of specific DNA sequences using alternately binding quenching probe competitive assay combined with loop - mediated isothermal amplification J. Anal Chem Hidenori Tani Takahiro Kanagawa Shinya Kurata et al. Quantitative J * Brugada BrS PCCD IVF Q71 Q A doi /j. issn X Research Progress of the Sodium Channels LI Bao - zhu GAO Bing - miao WU Yong YU Jin - peng WU Xiao - sa ZHANGSUN Dong - ting LUO Su - lan * Key Laboratory of Tropical Biological Resources of Ministry of Education Hainan University Haikou China Abstract The electrical activity of the cells was one of the essential features of the life phenomena and ion channels were its structural and functional base. Therefore the research of the ion channels mechanism played a significant role in theory and reality. Many nervous system and cardiovascular diseases such as multiple sclerosis epilepsy cerebral apoplexy neuropathic pain brugada syndrome BrS progressive cardiac conduction defects PCCD idiopathic ventricular fibrillation IVF etc were concerned with the alterations in the amino acid sequences and structures. The research progress of the sodium channels was discussed. Key words sodium channels sodium channel diseases sodium channel drugs research methods research progress No. IRT1123 ZDXM voltage gated channels ligand gated channels Hjkj * mechanogeted channels Tel luosulan2003@ 163. com 3

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