psl1 , 90%~95%. (Oryza sativa L) *,

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1 psl1 * * (,, *, ricegb@yzu.edu.cn).. 11 Co F 1 F 2,., psl1, SSR psl1 2., 34 STS, psl1., psl1 BAC, 48 kb,. (Oryza sativa L), 90%~95%.,.,, [1,2].,., [3]., 1 d, 2%, 1% [1,4].,.,,.,.,., RNA,. [1~6]., : ( ), ; ( ). [2,5~17],, [6].,. T-DNA cdna, [17~30].,,,., 10, 3 [31,32] 1), Li [33] pse(t), kb.. 1 ( ). 11 Co 60,,. 6 1)

51 20 2006 10 11, ; 11. ( ). 2004 6 11, F 1, F 2. 2005 F 2, ;, F 2,.,, F 2. F 2,, F 3. ( ). Michelmore [34]. F 2 2, 10 DNA, 2. 2 ;,. ( ) DNA SSR. F 2, 6~8 cm. F 2 326 (psl1). DNA CTAB [35].

2 , ; 11. ( ) , F 1, F F 2, ;, F 2,.,, F 2. F 2,, F 3. ( ). Michelmore [34]. F 2 2, 10 DNA, 2. 2 ;,. ( ) DNA SSR. F 2, 6~8 cm. F (psl1). DNA CTAB [35]., SSR µl Mg 2+ (25 mmol/l) 2.5 µl, 10 PCR ( Mg 2+ ) 2.5 µl, dntp (2.5 mmol/l) 2.5 µl, (15 ng/µl) 2.0 µl, DNA ( 15 ng/µl) 2µL, Taq (5 U/µL) 0.2 µl, 13.3 µl. Biometra PCR. : 94 5 min; 94, 1 min; 55, 1 min; 72, 1.5 min; 32 ; min. 4%, (EB) UVP. ( ) STS. BLAST, (Nipponbare) 9311 ; Primer bp 200~500 bp PCR, ;,,. ( ). 1 2, ( ) 2, MAPMAKER (EXP3.0b) [36], , ( 1),,. 25~30 d,, ,,,. 6 11, F 1, 6 11,. F 2,

3 ,. F ( 1), 1.,, psl1 (presescing leaf). 2.3 psl1, SSR, 2 SSR, 2, F 2 SSR , 6 2 (RM6, RM5303, RM5474, RM425, RM406 RM166),. RM6, RM5303, RM5472, RM425, RM406 RM166,, psl1, RM6-RM5303-RM5472-sl1- RM425-RM406-RM166. psl1 RM5472 RM425, RM5472 RM cm ( 2). RM5472 RM psl psl1,. psl1 RM5474 RM425, RM5472 RM cm. BAC 34 STS,, 9 ( 2) F 1, F 2 P 1 P 2 F 1 F 2 / χ 2 (3 1) / / RM5472 RM425 F 2 P1, ; P2,

4 psl1 STS STS2-19 STS2-26, cm., STS2-25( 4). STS2-19 STS (5 3 ) BAC STS2-5 F ATTTGGTTTGATTTTATTCC AP R TCTTGGTATGTGCTCACTTT STS2-6 F TGCTGTGGTTGTTCCTGTC AP R TTTAGGTTATGTTTACAAGAGGC STS2-9 F GGGAACTAAACCAAGCCTAA AP R CATCCGCATCGTAGTATTCA STS2-11 F ATCTGATGGGCGAACAACCT AP R TGGTCGTCGTCACTGTTGGA STS2-18 F CAGAGCAGCCTACCTAGCAG AP R TTCAGCCTTGACAAACCATC STS2-19 F CTGCGACATCTTCTGGCTAA AP R GCAGGCAGAAAGCGTACTAAC STS2-25 F TGCGACATCTTCTGGCTAA AP R CAGGCAGAAAGCGTACTAAC STS2-26 F GATGGTGGGACTGGCTAGTGT AP R TGTGGTGGTTATGTTGGGAAG STS2-28 F TCTCCCGATACATTCTCAA AP R ATACGGCATACAACCAAAC 2.5 psl1 2, RM6535 BAC AP005297, STS2-18 STS2-19 BAC AP004114, STS2-26 STS2-28 BAC AP , psl1 ( 6). AP005297, AP AP BAC, BAC, psl1 STS2-19 STS2-26, cm. STS2-19 AP004114, STS2-26 BAC 4 psl1 2 AP005733,., 48 kb, psl1, psl1. 3,,,.., ;, Rubisco [7].. 5 STS2-19 STS2-25 F 2 P1, ; P2,

5 psl1, [13].,, 25 d, 50~60 d; ; ;.. : ( ), ; ( ),., H3 [37], (pheophorbide a oxygenase).. [38]. [39],., hys1 (hypersenescence 1), cpr5 (constitutive expresser or pathogenesis related gene 5). [40]. 3 ore1, ore3 ore9. ore9, F-box [41].., Buchanan-Wallaston [13] ,,. Lin [6],,. cdna-aflp [13]., 11,, (psl1). STS-19 STS2-26, STS cm, STS cm, STS2-25., psl1 BAC, psl1 AP AP kb,. ( : ) ( : 2003CB CB120807) ( : 05KAJ2012). 1,,.., 2005, 21(3): ,..,

6 , 38(4): ,,.., 1997, 23(2): ,.., 1993, 14(4): ,.., 2003, 1(3): Lin J F, Wu S H. Molecular events in senescing Arabidopsis leaves. Plant J, 2004, 39: Stessman D, Miller A, Spalding M, et al. Regulation of photosynthesis during Arabidopsis leaf development in continuous light. Photosynthesis Res, 2002, 72: Hensel L L, Grbic V, Baumgarten D A, et al. Developmental and age-related processes that influence the longevity and senescence of photosynthetic tissues in Arabidopsis. Plant Cell, 1993, 5: Lohman K N, Gan S, John M C, et al. Molecular analysis of natural leaf senescence in Arabidopsis thaliana. Physiol Plant, 1994, 92: Oh S A, Lee S Y, Chung I K, et al. A senescence-associated gene of Arabidopsis thaliana is distinctively regulated during natural and artificially induced leaf senescence. Plant Mol Biol, 1996, 30: Park J H, Oh S A, Kim Y H, et al. Differential expression of senescence-associated mrnas during leaf senescence induced by different senescence inducing factors in Arabidopsis. Plant Mol Biol, 1998, 37: Buchanan-Wollaston V. Isolation of cdna clones for genes that are expressed during leaf senescence in Brassica napus. Plant Physiol, 1994, 105: Buchanan-Wallaston V, Simon E, Elizabeth H, et al. The molecular analysis of leaf senescence: A genomics approach. Plant Biotechnol J, 2003, 1: Hanfrey C, Fife M, Buchanan-Wollaston V. Leaf senescence in Brassica napus: Expression of genes encoding pathogenesis-related proteins. Plant Mol Biol, 1996, 30: Becker W, Apel K. Differences in gene expression between natural and artificially induced leaf senescence. Planta, 1993, 189: Kleber-Janke T, Krupinska K. Isolation of cdna clones for genes showing enhanced expression in barley leaves during dark-induced senescence as well as during senescence under field condition. Planta, 1997, 203: Smart C M, Hosken S E, Thomas H, et al. The timing of maize leaf senescence and characterization of senescence-related cdnas. Physiol Plant, 1995, 93: Feng Q, Zhang Y, Hao P, et al. Sequence and analysis of rice chromosome 4. Nature, 2002, 420: Goff S A, Ricke D, Lan T H, et al. A draft sequence of the rice genome (Oryza sativa L. ssp. japonica). Science, 2002, 296: Kikuchi S, Satoh K, Nagata T, et al. Collection, mapping and annotation of over cdna clones from Japonica rice. Science, 2003, 301: International Rice Genome Sequencing Project. The map-based sequence of the rice genome. Nature, 2005, 436: Jiang G H, He Y Q, Xu C G, et al. The genetic basis of stay-green in rice analyzed in a population of doubled haploid lines derived from an indica by japonica cross. Theor Appl Genet, 2004, 108(4): Lin L, Liu Y G, Xu X, et al. Efficient linking and transfer of multiple genes by a multigene assembly and transformation vector system. Proc Natl Acad Sci USA, 2003, 100: The Rice Chromosome 10 Sequencing Consortium. In-depth view of structure, activity, and evolution of rice chromosome 10. Science, 2003, 300: Sasaki T, Matsumoto T, Yamamoto K, et al. The genome sequence and structure and rice chromosome 1. Nature, 2002, 420: Shimamoto K, Kyozuka J. Rice as a model for comparative genomics of plants. Annu Rev Plant Biol, 2002, 53: Xue Y, Li J, Xu Z. Recent highlights of the China Rice Functional Genomics Program. Trends Genet, 2003, 19: Yu J, Hu S, Wang J, et al. A draft sequence of the rice genome (Oryza sativa L. ssp. indica). Science, 2002, 296: Zhang Y, Huang Y, Zhang L, et al. Structural features of the rice chromosome 4 centromere. Nucleic Acids Res, 2004, 32: Li X, Qian Q, Fu Z, et al. Control of tillering in rice. Nature, 2003, 422: Jung K H, Hur J, Ryu C H, et al. Characterization of a rice chlorophyll-deficient mutant using the T-DNA gene-trap system. Plant Cell Physiol, 2003, 44(5): Lee S, Kim J H, Yoo E S, et al. Differential regulation of chlorophyll a oxygenase genes in rice. Plant Mol Biol, 2005, 57(6): Li F Z, Jin S H, Hu G C. Isolation and physiological characteristics of a premature senescence mutant in rice (Oryza sativa L). J Zhejiang Univ Sci, 2005, 6B(8): Michelmore R W, Paran I, Kesseli R V. Identification of markers linked to disease-resistance genes by bulked segregant analysis: A rapid method to detect markers in specific genomic regions by using segregating populations. Proc Natl Acad Sci USA, 1991, 88(21): ,,,. sd-g., 2004, 49(8): Lander E S, Green P, Abrahamson J, et al. MAPMAKER: An interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics, 1987, 1: Thomas H, Schellenberg M, Vicentini F, et al. Gregor Mendel s green and yellow pea seeds. Bot Acta, 1996, 109: Thomas H. Sid a Mendelian locus controlling thylakoid disassembly in senescing leaves of Festuca pretensis. Theor Appl Genet, 1987, 73: Cha K W, Lee Y J, Koh H J, et al. Isolation, characterization, and mapping of the stay green mutant in rice. Theor Appl Genet, 2002, 104(4): Yoshida S, Ito M, Nishida I, et al. Identification of a novel gene HYS1/CRG5 that has a repressive role in the induction of leaf senescence and pathogen-defense responses in Arabidopsis thaliana. Plant J, 2002, 29: Woo H R, Chung K M, Park J H, et al. ORE9, an F-box protein that regulates leaf senescence in Arabidopsis. Plant Cell, 2001, 13(8): ( , )

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SCIENTIA SILVAE SINICAE. a/ b. oldhamii). The length of cab2bo1 ( GenBank accession

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