140 1674-6309 2017 02-0140-04 1 2 1 AhR rs2066853-205 190 - AS-PCR AhR rs2066853 AhR rs2066853 χ 2 AhR rs2066853 OR 95%CI AhR rs2066853 AA AG GG χ 2 =6.48 P<0.05 G A AG GG OR 1.43 95%CI 0.88~2.18 2.37 95%CI 1.22~5.50 AhR rs2066853 G AhR R589.2 A DOI:10.16050/j.cnki.issn1674-6309.2017.02.006 coronary heart disease CHD AhR 40% [1] 1 [2] 1.1 atherosclero- 2006 8-2006 11 sis AS 205 135 [3] 70 52.27±5.3 [ 4] 1 2 [5] 2~6 / 3 4 polycyclic aromatic hydrocarbons PAHs 190 108 82 52.69±5.2 [6] PAHs aryl hydro- 1 2 carbon receptor AhR α 3 LXRα 4 [7] AhR rs2066853 [8-10] / B AhR AhR rs2066853 1980- E-mail hyjiao1602@hotmail.com 1.2 1.2.1 DNA 3mL
2. rs2066853 141 Na2EDTA - 2A 2B 3 DNA TNE -20 DNA 5μL DNA 195μL 40 260nm 280nm OD OD260/OD280 1.8~2.0 DNA 1.2.2 Tag 2a 2b AG 3a 3b GG 10 Buffer Mg 2+ dntps DNA Maker 1 AhR rs2066853 AS-PCR Gold view 1.3 SPSS 17.0 Tris Gene Amp PCR System 9700 Gel Doc2000 χ 2 P 0.05 BIO-RAD 2 BIO-RAD 2.1 Hardy-Weinberg 1.2.3 Primer 5.0 AhR rs2066853 Hardy -Weinberg χ 2 =0.28 P > 0.05 2.2 AhR rs2066853 10μmol μl -1-20 F1 5 -GGCATTGAT TTTGAAGACATCAG-3 F2 5 -GGCATTGATTT TGAAGACATCAA-3 χ 2 =6.48 P<0.05 A G χ 2 =3.37 50μL 10 buffer P>0.05 AhR rs2066853ag Mg 2+ dntps Tag GG DNA 5 1 AA 1.5 1.5 1 35 5.0μL :94 OR 1.43 95%CI: 0.88~2.18 5min 95 35s 64.3 35s 72 2.37 95%CI: 1.22~5.50 1min 30 72 7min PCR 1.5% OR 1.58 95%CI 0.81~2.26 100bp AS-PCR rs2066853 1 2 PCR 1 3 AS-PCR DNA AS-PCR PCR 2 DNA 1 SNPs n=205 AhR rs2066853 % AhR rs2066853 A/A 66 31.8 81 42.6 Marker 500/400/300/200/100/25bp 1a 1b AA AhR rs2066853 AA AG GG n=190 χ 2 P OR 95%CI A/G 97 50.6 90 47.4 1.43 0.88~2.18 6.48 <0.05 G/G 42 17.6 19 10.0 2.37 1.22~5.50 A/G+G/G 139 68.2 109 57.4 1.58 0.81~2.26 1.0
142 39 2 AhR rs2066853 % SNPs n=205 n=190 χ 2 P OR 95%CI AhR rs2066853 A 114 55.6 100 52.6 1.0 G 91 44.4 90 47.4 3.37 >0.05 1.51 0.97~2.05 AhR rs2066853 G [11] AhR AhR AhR 7p15 AhR 1 National Health and Family Planning Commission. 50kb 11 10 China Statistical Yearbook of Health and Family Planning M. Beijing: Beijing Union Medical University 96kDa /Per-Arnt- Press 2015. Sim AhR 2 Finegold JA Asaria P Francis DP. Mortality from ischaemic heart disease by country region and age: [12] AhR -p - tetrachlorodibenzo-p-dioxin TCDD Nations J. Int J Cardiol 2013 168 2 :934-945. statistics from World Health Organisation and United AhR 3 Shah S Casas JP Gaunt TR et al. Influence of common genetic variation on blood lipid levels cardio- [13] AhR vas cular risk and coronary events in two British AhR prospective cohort studies J. Eur Heart J 2013 34 [14] 13 :972-981. AhR AhR 4 Ye H Li X Wang L et al. Genetic associations with AhR TCDD coronary heart disease: meta-analyses of 12 candidate genetic variants J. Gene 2013 531 1 :71-77. [15] AhR 10 5 Bullen C. Impact of tobacco smoking and smoking rs2066853 G>A rs2066853 G>A cessation on cardiovascular risk and disease J. Expert Rev Cardiovasc Ther 2008 6 6 :883-895. AhR 10 AhR rs2066853 6 Iwano S Asanuma F Nukaya M et al. CYP1A1-mediated mechanism for atherosclerosis induced by poly- [16] 939 cyclic aromatic hydrocarbons J. Biochem Biophys Res Commun 2005 337 2 :708-712. AhR rs2066853 7 Iwano S Shibahara N Saito T et al. Activation of p53 [ 17] as a causal step for atherosclerosis induced by poly - rs2066853 cyclic aromatic hydrocarbons J. FEBS Lett 2006 [18] 554 R554K 580 3 :890-893. [12] SNP 8 Wang XW Li K Guo S et al. The association of AhR AhR554 functional polymorphisms in the aryl hydrocarbon receptor AHR gene with the risk of vitiligo in Han P4501A1 cytochrome P450 1A1 CYP1A1 Chinese populations J. Br J Dermatol 2012 166 5 : CYP1A1 Msp 1081-1087. 9 Chen D Tian T Wang H et al. Association of human C aryl hydrocarbon receptor gene polymorphisms with [19] risk of lung cancer among cigarette smokers in a Chinese population J. Pharmacogenet Genomics 2009 AhR rs2066853 AA AG GG 19 1 :25-34. 10 Sangrajrang S Sato Y Sakamoto H et al. Genetic polymorphisms of estrogen metabolizing enzyme and AhR AhR breast cancer risk in Thai women J. Int J Cancer AhR 2009 125 4 :837-843.
2. rs2066853 143 11 Humblet O Birnbaum L Rimm E et al. Dioxins and cardiovascular disease mortality J. Environ Health Perspect 2008 116 11 :1443-1448. 12 Brokken LJ Giwercman YL. Gene-environment interactions in male reproductive health: special reference to the aryl hydrocarbon receptor signaling pathway J. Asian J Androl 2014 16 1 :89-96. 13 Frericks M Burgoon LD Zacharewski TR et al. Promoter analysis of TCDD-inducible genes in a thymic epithelial cell line indicates the potential for cell - specific transcription factor crosstalk in the AhR response J. Toxicol Appl Pharmacol 2008 232 2 : 268-279. 14 Kikuchi Y Ohsawa S Mimura J et al. Heterodimers of bhlh -PAS protein fragments derived from AhR AhRR and Arnt prepared by coexpression in Escherichia coli characterization of their DNA binding a ctivity and preparation of a DNA complex J. J Biochem 2003 134 1 83-90. 15 Arsenescu V Arsenescu RI King V et al. Polychlorinated Biphenyl -77 Induces adipocyte differentiation and proinflammatory adipokines and Promotes obesity and atherosclerosis J. Environmental Health Perspectives 2008 116 6 :761-768. 16 Harper PA Wong JM Lam MS et al. Polymorphisms in the human AH receptor J. Chem Biol Interact 2002 141 1-2 :161-187. 17 Huang S Shui X He Y et al. AhR expression and polymorphisms are associated with risk of coronary arterial disease in Chinese population J. Sci Rep 2015 5:8022. 18 Safarinejed MR shafiei N Safarinejad S. Polymorphisma in arg hgdrocarbon receptor gene are associated with idiopathic male factor infertility J. Reporod Sci 2013 2002 20 :161-187. 19 Wang XL Greco M Sim AS et al. Effect of CYP1A1 MspI polymorphism on cigarette smoking related coronary artery disease and diabetes J. Atherosclerosis 2002 162 2 :391-397. AhR rs2066853 is Associated with Coronary Heart Disease MA Jia Dai Xiaojing JIAO Haiyan Abstract: Objective χ Key words: