Effects of Plan t Growth Regula tors on the Con ten t of Phenolics in Sweet Cherry D orman t Flower Buds and D ormancy

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1 2005, 32 (4) : Acta Horticulturae Sinica (, ) : 7 ( Prunus avium L. ),,, ABA, 62BA GA 3 ; ABA, 62BA GA 3, ; ABA, 62BA GA 3 62BA CA 3, CA 3 ABA ( PAL) ( PPO), 62BA GA 3 PAL PPO, 62BA GA 3,, 50%, ; ABA, : ; ; ; : S : A : X (2005) Effects of Plan t Growth Regula tors on the Con ten t of Phenolics in Sweet Cherry D orman t Flower Buds and D ormancy W ei Hairong, Gao Dongsheng 3, and L i Xianli ( Institute of Horticulture Science and Engineering, Shandong A gricultural U niversity, Taiπan , China) Abstract: The experiment was conducted with 72year2old Hongdeng and Zaohongbaoshi sweet cherry ( P runus avium L. ) trees, and effects of p lant growth regulators on the content of phenolics and relate enzymes activity and rate of germ ination were analyzed and studied. Results showed that the effects of different p lant growth regulators were different: ABA p romoted the accumulation of phenolics 62BA and GA 3 postponed the accumulation of phenolics in the p rimary stage; In the m iddle stage, ABA kep t the content of phenolics high while 62BA and GA 3 p romoted the content of phenolics peak then it dropped; In the last period, ABA postponed the drop of the phenolics content, 62BA and GA 3 were contrary to ABA. The effect of CA 3 was better than that of ABA. ABA made PAL activity raise, and dropped the PPO activity; 62BA and GA 3 made PAL dropped but raised the PPO activity. Effects of different p lant growth regulators on the germ ination rate were different in dif2 ferent periods of dormancy. In the p rimary stage, 62BA and GA 3 on the rate of germ ination were not obvious; In the m iddle stage, 62BA and GA 3 can break dormancy and made the rate of germ ination more than 50% ; In the last period, effects of 62BA and GA 3 were sim ilar to the m iddle of stage. In the whole period of dormancy, ABA made the rate of germ ination lowerd and inhibited the dormancy2release. Key words: Sweet cherry; Plant grow th regulator; Phenolics; Dormancy,,, : ; : : 863 (2001AA247041) 3 Author for correspondence ( E2mail: dsgao@ sdau1edu1cn)

2 4 : 585, 1, 2,,,, 3,, ( P runus avium L. ), ABA 100 mg L BA 100 mg L - 1 GA mg L - 1,, 3 4 d 50 70,,,, 20 m in, ( - 20 ), Folin -, d50 ( PAL ) , 4, ABA 100 mg L BA 100 mg L - 1 GA mg L - 1,,, 2 d 1, ( 25 / 12, ), 25 d, 50%, ( 1), ,,, ABA,, 62 BA GA 3, GA 3, 62BA ABA, 62BA GA , ABA ; 62BA GA 3, GA 3 62BA , 12 11, ,, ABA, ; 62BA GA 3,, ABA ; 62BA GA 3

3 ( 11-04) 2 ( 11-29) F ig. 1 The effect of plan t growth regula tors on phenolics F ig. 2 The effect of plan t growth regula tors on phenolics con ten t in the early stage of dormancy ( 11-04) con ten t in the m iddle stage of dormancy ( 11-29) 3 ( 12-27) F ig. 3 The effect of plan t growth regula tors on phenolics con ten t in the la st stage of dormancy ( 12-27) 4 ( PAL ) ( PPO ) F ig. 4 The effect of plan t growth regula tors on PAL and PPO activ ities during dormancy 214 PAL PPO 4, PAL, 3 : GA 3 62BA PAL, GA 3 ; ABAPAL3 1% 4, PPO ABA PPO,, GA 3 62BA PPO,, 3

4 4 : 587 1% 215 1, 11 4, 3 ABA,,, 3 ; 11 29, GA 3 62BA, 56134% 52121%, 1%, ABA, ABA ; 12 29,, 64121%, 3 50% 1 Table 1 The effect of plan t growth regula tors on the germ ina tion ra te of Hongdeng and Zaohongbaosh i Period (M - D) dur ing the dormancy period ( % ) Treatment Hongdeng Zaohongbaoshi ABA 10162bB 11102bB GA aA 15132aAB 62BA 18126aA 16157aA Control 18157aA 16189aA ABA 9132dD 9123cC GA aA 58197aA 62BA 52121bB 54132bB Control 10165cC 9178cC ABA 64128cC 75136bB GA aA 95167aA 62BA 86132bB 92136aA Control 64121cC 75195bB,, GA 3 62BA 3,, ABA GA 3 ABA 7, 8,, ABA,, ABA GA 3 62BA,,,,, 50% ABA, GA 3 62BA, ( PAL) 9, ABA PAL, PPO,,, GA 3 62BA,, 7, 10, 11 : 1,.., 1990, 14 (1) : 7 15 Zhu Y L, W u G M. Analysis for endogenous hormones in pop lar bud during dormancy and its release. Journal of Nanjing Forestry Universi2 ty, 1990, 14 (1) : 7 15 ( in Chinese) 2,,,,. ABA GA 3., 2004, 31 ( 2) :

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