2.1. (Klein et al. 2000) LGG LGG LGG. al. 2002)

Transcript

1 DOI: /j.mfst s1.017 ATCC (Klein et al. 2000) ATCC Sherwood Gorbach Hudault 1997 Caco-2 Hela Barry Goldin Lactobacillus ( Mattar et al. 2002) Gorbach Goldin Caco (Mattar et al. 2001) 30 (Banasaz et 1972~ (Ruseler-van Embden et al. 1995a; Ruseler-van Embden et al. 1995b) Caco-2 HT-29 (Chen et al. 1993) 2.2 al. 2002) 55

2 (Donohue et al ) 5192 (Lee et al. 2000) 56 (Naaber et al Wagner et al. 1997) 2.3 (1) (2) (3) (4) (5) (Korpela et al. 1997) (6) (7) (8) (Gupta et al. 2000; Malin 1997) 2002 Vanderhoof (Valtonen et al. Document of Helsinki University Central Hospital, 2003) kg % Oberbelman (Saxelin et al. 1996) 1989 Young

3 Oksanen Hilton Lactophilus Lactophilus 3.3 IgA 4.1 Majamaa 4.3 L A IgA 3.5 β p- 3.6 Meurman 4.2 ph3.0 4 L 57

4 58 30 Evaluation of probiotic treatment in a neonatal animal model. Pediatr Surg Int 16: Benno, Y., HE, F., et al Effect of Lactobacillus GG yoghurt on human intestinal microecology in Japanese subjects. Nutrition Today 31 (6) Suppl. 1: 9S-11S. 2 Donohue DC, Deighton M, Ahokas JT, Salminen S (1993) Toxicity of lactic acid bacteria El-Nezami, H.,et al a. Ability of dairy strains of lactic acid bacteria to bind a common food carcinogen, aflatoxin B1. Food Chem. Toxicol. 36: Goldin, B.R., Gorbach, S.L., Saxelin, M., et al Survival of Lactobacillus species (strain GG) in human gastrointestinal tract. Dig. Dis. Sci. 37 (1): Guarino A et al Oral bacterial therapy reduces the duration of symptoms and of viral excretion in children with mild diarrhoea. J Pediatr Gastroenterol Nutr 25: Gupta P, Andrew H, Kirschner BS, Guandalini S (2000) Is lactobacillus GG helpful in children with Crohn's disease? Results of a preliminary, open-label study. J Pediatr Gastroenterol Nutr 31: Hilton E, Kolakowske P, Smith M and Singer C Efficacy of Lactobacillus GG as a diarrheal preventative in travellers. J Travel Med 4: Hudault, S.,et al Antagonistic activity excerted in vitro and in vivo by Lactobacillus casei (strain GG) against Salmonella typhimurium C5 infection. Appl. Environ. Microbiol. 63: Jukka Meurman et al offers dental care. Action. 2: Kaila, M, Isolauri, E., et al Fecal recovery of a human Lactobacillus strain (ATCC 53103) during dietary therapy of rotavirus diarrhea in infants. Biosci. Microflora 17 (2): Klein G, et al (2000) Exclusion of vana, vanb and vanc type glycopeptide resistance in strains of Lactobacillus reuteri and Lactobacillus rhamnosus used as probiotics by polymerase chain reaction and hybridization methods. J Appl Microbiol 89: Korpela R, Moilanen E, Saxelin M, Vapaatalo H (1997) Lactobacillus rhamnosus GG (ATCC 53103) and platelet aggregation in vitro. Int J Food Microbiol 37: Lee DJ, Drongowski RA, Coran AG, Harmon CM (2000) 14 Malin M Evaluation of the intestinal mucosal barrier in Crohn s disease and juvenile chronic arthritis. MD Tehsis. Acta Universitatis Tamperensis. Ser A Vol Mattar AF, Drongowski RA, Coran AG, Harmon CM (2001) Effect of probiotics on enterocyte bacterial translocation in vitro. Pediatr Surg Int 17: Mattar AF, Teitelbaum DH, Drongowski RA, Yongyi F, Harmon CM, Coran AG (2002) Probiotics up-regulate MUC-2 mucin gene expression in a Caco-2 cell-culture model. Pediatr Surg Int 18: Millar MR, Bacon C, Smith SL, Walker V, Hall MA (1993) Enteral feeding of premature infants with Lactobacillus GG. Arch Dis Child 69: Naaber P, Mikelsaar RH, Salminen S, Mikelsaar M (1998) Bacterial translocation, intestinal microflora and morphological changes of intestinal mucosa in experimental models of Clostridium difficile infection. J Med Microbiol 47: Oberhelman RA, Gilman RH et al A placebo-controlled trial of Lactobacillus GG to prevent diarrhea in undernourished Peruvian children. J Pediatr 134: Oksanen P, Salminen S, Saxelin M, etc Prevention of traveller s diarrhoea by Lactobacillus GG. Ann Med 22: Pant AR, et al Lactobacillus GG and acute diarrhoea in young children in the tropics. J Tropic Pediatrics 42: Ruseler-van Embden JG, van Lieshout LM, Gosselink MJ, Marteau P (1995a) Inability of Lactobacillus casei strain GG, L. acidophilus, and ifidobacterium bifidum to degrade intestinal mucus glycoproteins. Scand J Gastroenterol 30: Ruseler-van Embden JGH, van Lieshout LM, Marteau PM (1995b) No degradation of intestinal mucus glycoproteins by Lactobacillus casei strain GG. Microecology and Therapy 25: Saxelin M, Chuang NH, Chassy B, Rautelin H, Makela PH, Salminen S, Gorbach SL (1996) Lactobacilli and bacteremia in southern Finland, Clin Infect Dis 22: Saxelin, M., Elo, S., et al Dose response colonization of faeces after oral administration of Lactobacillus casei strain GG. Microb. Ecol. Health Dis. 4:

5 2001,6:31~ ~ ,1:43~ Nutrition.1998,80:203~ ,21:23~ casei preparation the recurrence of superficial bladder cancer 2001,21:13~15 10 Cherie J. Ziemer. An Overview of Probiotics, Prebiotics and Synbiotics in the Functional Food Concept: Perspectives and Future Strategies. Int. Diary Journal. 1998,2:473~ Indu Pal Kaur. Probiotics: Potential Pharmaceutical applicat- ions. Pharmaceutical Science. 2002, 15:1~9 12 Mary Ellen Sanders. Overview of Functional Foods: Emph- asis on Probiotic Bacteria Int. Diary Journal. 1998,2:341~ Seppo Salminen. Demonstration of Safety of Probiotic- a review. International Journal of Food Microbiology. 1998, 44: 14 Golden BR. Health benefits of Probiotic. British Journal of 15 Aso Y Akanzan H. Prophylactic effect of a Loctobacillus [J]. Urol Intl,1992,49:125~ Bellomo G, Mangiale A, Frigerio G. A controlled double- blinded study of SF68 strain as a new biological preparation for the treatment of diarrhea in pediatrics[j].curr Therapeutic Res.1980,28:927~ Edens F W et al. Purification and Characterization of glycrol dehy dratase from Lactobacillus reuteri. Appl. Environ. Microbial. 1997,76:179~196 The Mechanism, the Present Questions and the Countermeasure on Probiotics Shu Na 1 Rui Han-Ming 2 1.Fudan University School of Pharmacy, Shanghai, (2.South China University of Technology Food and Biological Engineering College, Guangzhou, ) Abstract: This paper deals with the mechanism, the health benefit to human and the present questions of probiotic, presenting the approaches to improve the efficiency of probiotic bacteria. Key words: probiotic; mechanism; question 26 Saxelin, M., Pessi, T., and Salminen, S Fecal recovery following oral administration of Lactobacillus strain GG (ATCC 53103) in gelatine capsules to healthy volunteers. Int. J. Food Microb. 25: Tuomola, E.M., Ouwehand, A.C., and Salminen, S The effect of probiotic bacteria on the adhesion of pathogens to human intestinal mucus. FEMS Immunol. Medic. Microbiol. 26: Wagner, R.D.,et al Biotherapeutic effects of probiotic bacteria on Candidiasis in immunodeficient mice. Infect. Immun. 65: Vanderhoof JA, Young RJ, Iyer K, Brown C, Horslen SP (2002). Safety of probiotics in pediatric live and bowel transplant patients. J Pediatr Gastroenterol Nutr 35: Young RJ, Whitney DB et al Prevention of antib iotic -associated diarrhea utilizing Lactobacillus GG. Gastroent Int 11 Suppl 1, p86. Review on studies of probiotics Wang Shibin, Liu Jinjie, Qiao Lan, Cheng Lin (Valio Ltd Shanghai Rep. Office, Shanghai, ) Abstract:, a patented L. rhamnosus strain (ATCC 53103), was discovered from healthy person by two American professors in early 1980 s. It is the best documentated probiotics in the world. The safety of is verified by many in vitro experiments, animal studies, clinical studies and epidemiological studies. Its functionalities include: improving GI tract function, preventing and cure diarrhea, regulating immune function, preventing allergies, binding and eliminating toxins, preventing dental caries, etc. In addition, compared with many other probiotics, has a lot of advantages, such as it can survive in low ph environment, colonize in GI tract, live remains almost constant in final products during shelf life, etc. Key words: probiotics; ; safety; functionality; advantage 70