Ct L1 L2/L2a L3. NGU human papilloma virus HPV Ct human immunodeficiency virus HIV PCR RFLP

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1 PCR-RFLP ~ % E 48 D 4 E Prevalence of chlamydia trachomatis urogenital tract infection and genotyping in out-patients of sexually transmitted disease clinics: a pilot study WANG Yan, FENG Wen-li, YANG Jing, WANG Yan-qing, JI Ying, YANG Hui-qing, ZHANG Rong-li. Department of Dermatology & Venerology, Second Hospital of Shanxi Medical U- niversity, Taiyuan , China Abstract Objective To investigate Chlamydia trachomatis infection and genotypes in out-patients of sexually transmitted disease (STD) clinics in Taiyuan. Methods We collected the urethral or cervical secretion of out-patients with susceptible urogenital tract C. trachomatis infection, between 2008 and 2011, for examination of C. trachomatis by using ClearviewTM antigen assay kit (UK). A subgroup of positive specimens were subjected to culturing and genotyping via polymerase chain reaction-restriction fraction length polymorphism. Results Of the 2387 specimens within the four years, 365 tested positive to C. trachomatis antigen. There was a trend towards reduced annual incidence of infection among the population, with a male-to-female ratio of 2.2:1 and peak age of onset of years. Of 137 clinical specimens sent for C. trachomatis culturing, 52 strains (48 of type E and 4 of type D) tested positive, thus resulting in a positive rate of 38.0%. Conclusion A comparatively high incidence rate of urogenital tract C. trachomatis infection, mostly genotyped as type E, in out-patients of STD clinic in Taiyuan deserves further large-scale study. Key words Chlamydia, trachomatis; Menbrane proteins; Genes; Polymorphism, restriction fragment length Chlamydia trachomatis Ct major outer membrane protein MOMP 19 A B/Ba C Ct L1 L2/L2a L3 D~K non-gonorrhoea urethriti NGU human papilloma virus HPV Ct human immunodeficiency virus HIV - PCR RFLP Ct 1

2 r/min 60 min NGU 2 h 3 ml 60~ 1 72 h 1 ml 2SP % 2~3 90% DNA 100 μl 1 ml Eppendorf r/min 20 min 50 mmol/l KCl 2.5 mmol/l MgCl 2 10 mmol/l Tis-HCl ph g/l 0.45%NP % mg/l CO h K 55 1 h 3 15 min K r/min 3 min PCR-RFLP omp1 MOMP CO 2 35~37 24~48 h 1077 bp 5 -ATGAAAAAACTCTTGAAATCGG TTTCTAGACTTCATCTTGTT-3 4 PCR 2 >4 5 6 Taq PCR Master Mix 12.5 μl DNA 5 μl s s s UNIPATH Clearview Ct 10 min 5 μl PCR 1% McCoy L Ct E 10 TBuffer BSA omp1 PCR Alu Msp MEM % 50 bp DNA Marker 2~3 cm 80 V 1.5 h 1~2 cm 30 s EB Ct D~ 1 K PCR-RFLP DNA 2SP Eppendorf 1.5 ml omp SPSS Ct Ct χ 2 P<0.05 > ~0.7 Ct 2.5 μl 94 5 min < Ct McCoy Ct Ct ml DEAE-D 30 min min 3000 r/min 15.3% Ct 5 min 5 ml χ 2 = P<0.01 1

3 % Ct Ct Ct ~ ~ 31~ 15.9% 15.6% 14.7% 19.5% 19.6% 12.5% 31~ Ct 19.5% 21~ 19.6% 41~ Ct 6.8% 8.9% % 21~ 41~ % Ct 2 / % % 0~ ~ ~ ~ ~ Ct % % % omp bp M E bp -250 bp -500 bp -750 bp bp M D2000 1~5 E E 98% 3 M E M 50bp DNA ladder E E 1~3 E 1 Ct omp1 Ct PCR-RFLP E 48 2 D 4 3 Ct BLAST omp1 CDC 2008 BLAST Ct Ct 120 Ct E/bour DQ D/B-185 X Ct E

4 M 1 2 M 50bp DNA ladder 1 2 D Ct D Ct Ct ~ ~ 21~ ECDC 2007 Ct /10 41~ 51 5%~10% 2 Ct 2007 Ct PCR-RFLP Ct omp1 5.85% omp1 Ct / kb PCR % 3 Ct 100% Ct 2388 Ct % %~20.3% % 76.9% 4.7% ~ 11 Ct 28.15% 4 7 D~K 2%~15% Ct UNIPATH Mossman 14 Ct Ct EIA H 15 D/Da J/Ja 16 PCR E RFLP D 48% E F 17 PCR PCR PCR-RFLP E 27.9% F PCR 23.5% G 12.4% D 11.1% E % 27.1% F % Ct E Ct 2 E D Ct Ct E

5 1260 E 2 workers in catalonia spain. Med Clin Barc E D RFLP 9 Ct E Centers for Disease Control and Prevention ED/OL. Presentation Chlamydia prevention challenges and strategies for reducing disease burden. http //www.cdc.gov/about/grand-rounds/archives/ 2010/05-May.htm 2 European Center for Disease Prevention and Control Chlamydia Folch C Sanclemente C Esteve A et al. Social characteristics risk behaviours and differences in the prevalence of HIV/sexually transmitted infections between Spanish and immigrant female sex Mossman D Beagley KW Landay AL et al. Genotyping of uro- Control in Europe ED/OL. http //ecdc.europa.eu/en/healthtopics/spotlight/chlamydia/pages/surveillance.aspgenital Chlamydia trachomatis in Regional New South Wales Australia. Sex Transm Dis Hsu MC Tsai PY Chen KT et al Genotyping of Chlamydia trachomatis ED/OL. http //www.ncstdc.org/yqjc-detail/work/2009/work_030.pdf from clinical specimens in Taiwan J Med Microbiol 4 Vall-Mayans M Villa M Saravanya M et al. Sexually transmitted 16 Bandea CI Debattista J Joseph K et al. Chlamydia trachomatis Chlamydia trachomatis Neisseria gonorrhoeae and HIV-1 infections in two at-risk populations in Barcelona female street prostitutes serovars among strains isolated from members of rural indigenous communities and urban populations in Australia. J Clin Microbiol and STI clinic attendees. Int J Infect Dis Singh V Salhan S Das BC et al. Predominance of Chlamydia 5 Gaydos C Maldeis NE Hardick A et al. Mycoplasma genitalium trachomatis serovars associated with urogenital infections in females compared to chlamydia gonorrhoea and trichomonas as an aetiological in New Delhi India. J Clin Microbiol agent of urethritis in men attending STD clinics. Sex Transm Infect Gao X Chen XS Yin YP et al. Distribution study of Chlamydia 6 Gaydos C Maldeis NE Hardick A et al. Mycoplasma genitalium trachomatis serovars among high-risk women in China performed as a contributor to the multiple etiologies of cervicitis in women using PCR-restriction fragment length polymorphism genotyping. attending sexually transmitted disease clinics. Sex Transm Dis J Clin Microbiol

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