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1 23 6 CHINA BIOTECHNOLOGY , ( ),,,20 90,, 863 DNA,,,,,,, 1,,, : (1),, ; (2), ; (3), ; (4) ; (5), ; (6) ; (7), [1] : : , com,,, B T B, IgA [2 ] IgA,,, Al, [3 ] 2 [4 ] : (1) ; (2),, ; (3),, ; (4) ; (5) ; (6) :,, ( TMV) ( McCormick, 1999) (CPMV) ( Brennan,1999), ; [5 ]

2 (CT) 500, 20 CT B (CT2B), Arakawa CT2B, CT2B,, lgm lgg lga3 CT, 1g 42 %, 3g 60 %,30 g CT2B 55 %, [6 ],,,, [7 ] , 50,MPT64,,, MPT64, MPT64 DNA [8 ] [6 ], MPT264 ESTA26, MPT264, MPT ( ETEC) ETEC B (LT2B) RSV Sandhu [17 ] LT2B,, 5 gπg, 30 gπg [9 ], lga lgglt2b ;, 3 ( glt2b), LT2B ( IgG) ( IgA), 5 g LT2B [5,10 ] ( HBV) Mason [11 ] HBsAg,,, 0101 % Thanavala [12 ], HBsAg, rhbsag B T, HBsAg,, Kapusta [13 ], (NV) NV,, NVCP NVCP, NVCP 0123 %, 0137 % [14 ] NVCP, IgG siga NVCP,, g NVCP,95 %lga,20 % lgg [15 ] (RSV) RSV Belanger a [16 ] RSVG, E8, RSV (RSVF),, RSVF RV,, VP7 VP4

3 6 : 33 [6 ] A G1 VP7, Kapikian 2 (RRV2TV) [18 ] GI Wa VP7 pcona311,, Wa ( HCMV) B (UL55) HCMV, Tackaberry [19 ] HCMV B, B, ngΠmg, 658ng TGEV ( FMDV),Carrillo VPI VPI,, VPI,, [20 ] VPI,,, [21 ] (RV), Mcgarvey Rv,, [6 ],Modelska, 3,40 %, IgG lga [22 ] (HA),, [23 ], Westernblot 83 %, 0103 % 0104 % S ( TGEV2S) Gomez [24] TGEV2S N (NgS), TGEV2S, TGEV lgg,s TGEV2S N cdna cdna [25], 0103 % 0106 % Tuboly [26] ( RHDV) VP60 VP60 RHDV,Castanon [27 ] RNDV VP60, 35S(CaM35S), mrna VP60,, VP60,, VP60, 313, ZP3, Fitchen ZP3 13 TMV,,, ZP3, ZP3 314, Ma, (NOD)

4 , ( GAD67) 10Π 12 NOD GAD67 8Π12 :NOD CT2B,, [28 ] 4,,,,, : (1) [29 ], ;, ; ;, (2), (3) (4),, [30 ] (5), 5,, [ 1 ],, ,22 (1) :19 21 [ 2 ],,..,2001,43 (2) : [ 3 ]..,2001, 5 :36 40 [ 4 ].., 2001,4,26 28 [ 5 ],,..,2002,2 (4) [ 6 ],,..,2001,9 (3) : [ 7 ],.,, 1998,33 (9) [ 8 ] Leez2M ( ). The Contemporary Vaccine. Beijing : Higher Education Press. Western [ 9 ] Tacket Co, Mason HS, et al. Immunogenicity in humans of a recombinant bacterial antigen delivered in a transgenic potato. Nature MeDicine,1998,4 : [ 10 ] Mason HS, Haqta. Edible vaccine protect smice against Escberic biacoli heat2labileen terotoxin (LT) : potatoes expressing a synthetic LT2B gene[j ]. Vaccine,1998,16 : [ 11 ] Mason HS,Lam DMK,et al. Expression of hepatitis B surface antigen in transgenic plants. Proc Natl Acad Sci USA, 1992, 89 : [ 12 ] Thanavala Y, Yang Y F, et al. Immunogenicity of transgenic plant2 derived hepatitis B surface antigen. Proc Natl Acad Sci USA,1995, 92 : [ 13 ] Kapusta J,Modelska A,et al. A plant2derived edible vaccine against hepatitis B virus. FASEB J,1999,13 : [ 14 ] Tacket co, Mason HS, et al. Human immune responses to a novel Norwalk virus vaccine delivered in transgenic potatoes. J lefect Dis, 2000,182 (1) : [ 15 ] Mason. HS,Ball Jm,Shijj,et al. Expression of Norwalk virus capsid protain in transgenic tobacco and potato and its oral immunogenicity in mice. Proc Natl Acad Sci USA. 1996,93 (11) : [ 16 ] Belanger H,Fleysh N,Cox S,et al. Human respiratory syncytial virus vaccine antigen produced in plants. FASEBJ,2000,14 (14) : [ 17 ] Sandhu JS, Krasnyanski SF,Dominer LL,et al. Oral immunization of mice with transgenic tomato fruit expressing respiratory syncytial virus2 F protein induces a systemic immune response. Transgenic Res,2000, 9 (2) : [ 18 ],,.., [ 19 ] Tackaberry ES, Prion F,et al. Development of biopharmaceuticals in plant expression and immunological reactivity of human cytomegal virus glycoprotein B in seeds of transgenic tobacco. Vaccine,1999,17 (23224) : [ 20 ] Carrillo C, Wigdorovitz A, Oliveros JC, et al. Protective immune response to food2and2mouth disease virus with VPI expressed in transgenic plants.j Virol,1998,72 (2) : [ 21 ] Wigdorovitz A,Carrillo C,Dus Santos MJ,et al. Protection of mice against challenge with food and mouth disease virus by immunization with foliar extracts from plants infected with recombinant tobacco mosaic virus expressing the FMDV structural protein VP. Virology, 1999,264 (1) :85 91 [ 22 ] Modelska A, Dietzschold B, Sleysh N, et al. Immunization against rabies with plant derived antigen. Proc Natl Acad Sci USA,1998,95 (5) : [ 23 ],Hugh S Mason.., 2001,11,3 4 [ 24 ] Gomez,Wigdorovitz A,Castanon S,et al. Oral immunogenicity of the plant derived spike protein from swine2transmissible gastrce nteritis coronavirus. Arch Virol,2000,145 (8) : [ 25 ] Gomez N, Carrrillo C, Salinas J, et al. Expression immunogentic glycoprotein S polyeptides from transmissible gastroe nteritis coronavirus in transgentic plans. Virology,1998,249 : [ 26 ] Tuboly T, Yu W, Bailey A, et al. Immunogenicity of procine transmissible gastroenteritis virus spike protein expressed in plants.

5 6 : 35 Vaccine,2000,18 (19) : [ 27 ] Castanon S,Martin MS,Martin2Alonso JM,et al. Immunization with potato plants expressing VP60 protein protects against rab bit hemorrhagic disease virus.j Virol, 1999,73 (5) : [ 28 ] Ma SW,Zhao DL. Transgenic plants expessing autoantigens fed tomice to induce oral immune tolerance[j ]. Nature Med,1997,3 : [ 29 ].., 1999,4 :1 5 [ 30 ],.,,2002, 22, (2) The Application of Transgenic Plants as Bioreactor in Vaccine Ren Zhiqiang 1,3 Liu Huimin 2 Li Runzhi 3 Yang Huizhen 1 (1 Institute of Crop Genetics Shanxi Academy of Agricultural Sciences Taiyuan Shanxi Academy of Agricultural Sciences Taiyuan Shanxi Agricultural University Taigu ) Abstract In this review,the concept and the advantages and the effect mechanism and the methods of producting vaccines by transgenic plant,have been introduced. The progresses of studies in many kinds transgenic plant vaccines were generalized. The problems and the foreground facing in the studies of transgenic plant vaccines are pointed out. Key words Transgenic plant Bioreactor Vaccine (30 ) [ 29 ] Sweigard JA, Carroll AM, Farrall L, et al. Magnaporthe grisea pathogenicity genes obtained through insertional mutagenesis. Mol Plant Microbe Interact,1998,11 (5) : [ 30 ] Hamer L, Adachii K, Montenegro2Chamorro MV, et al. Gene discovery and gene function assignment in filamentous fungi. Proc Natl Acad Sci USA,2001,98 : [ 31 ] Rho HS, Kang S, Lee YH. Agrobacterium tumefaciens2mediated transformation of the plant pathogenic fungus,magnaporthe grisea. Mol Cells,2001,12 (3) : [ 32 ] Sweigard JA,Ebbole DJ. Functional analysis of pathogenicity genes in a genomics world. Curr Opin Microbiol,2001,4 : [ 33 ] Konishi H, Ishiguro K, Komatsu S. A proteomics approach towards understanding blast fungus infection of rice grown under different levels on nitrogen fertilization. Proteomics,2001,1 : [ 34 ] Hamer L, Pan H, Adachi K, et al. Regions of microsynteny in Magnaporthe grisea and Neurospora crassa. Fung Genet Biol,2001, 33 : [ 35 ] Liu G,Lu G, Zeng L, et al. Two broad2spectrum blast resistance genes,pi9 (t) and Pi2 (t),are physically linked on rice chromosome 6. Mol Genet Genomics,2002,267 (4) : Avirulence Genes in Magnaporthe grisea Li Hongyu 1 Lu Guodong 1 Wang Zonghua 1,2 (1 College of Plant Protection Fujiang Agriculture and Forestry Universty Fuzhou ) (2 College of Life Sciences Fujiang Agriculture and Forestry University Fuzhou ) Abstract Avirulence gene encodes elicitor which triggers specific interaction of plant and pathogen. It is known that rice2blast fungus interaction complies with the gene2for2gene relationship. We summarized the progress in research on avirulence genes of the rice blast fungus ( Magnaporthe grisea), including the importance of the research,avirulence genes cloned and their interaction characteristics with rice resistance genes. Key words Magnaporthe grisea (anamorph : Pyricularia grisea) Avirulence gene Gene for gene theory Rice ( Oryzae sativa)

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JOURNAL OF CHINESE BIOTECHNOLOGY ( ) ,,,,,,, ,,, (molecular farming), ,,, [3 ] 3 (No. C010903) public. hk. hi. cn

22 5 JOURNAL OF CHINESE BIOTECHNOLOGY 2002 10 3 3 3 ( 571101),, [1 ],,,,,,,, 1 111 (molecular farming),,,,,, 3 (No. C010903) 3 3,: (0898) 66890695,:nkbltcsx @ public. hk. hi. cn 112,,,,,, [2 ],,,,, [3