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1 Med J Chin PLA, Vol. 43, No. 3, March 1, [ ] ( 500m n=193) ( 500m) 2h ( 3700m) 1d( n=147) 7d( n=122) 3 (PLT) (PDW) (PCT) (MPV) (P<0.05)PLT PDW PCT (P<0.05) (PAF) (Epi) (P<0.05) PAF Epi (P<0.05) (5-HT) 3 (P>0.05) Pearson PLT (SpO 2 ) (P<0.05) PLT PDW PCT SpO 2 [ ] [ ] R339.54; R821.52; R [ ] A [ ] (2018) [DOI] /j.issn Influence of acute high altitude exposure and short-term acclimation on platelet-associated parameters in healthy young man KE Jing-bin, LI Jia-bei, ZHANG Ji-hang, BIAN Shi-zhu, YANG Jie, LIU Chuan, CHEN Can, ZHANG Chen, YU Jie, JIN Jun, HUANG Lan* Department of Cardiology, Xinqiao Hospital, Army Medical University, Institute of Cardiovascular Diseases of PLA, Chongqing , China * Corresponding author, huanglan260@126.com This work was supported by the National Natural Science Foundation of China ( , ) [Abstract] Objective To explore the effect of high altitude exposure and short-term acclimation on the platelet-associated parameters by studying the changes of platelet-associated parameters in healthy young man. Methods Four hundred and sixtytwo young men were recruited from Chengdu (the elevation of 500m) during Jun to Aug according to the inclusion and exclusion criteria, of which 193 had been living in Chengdu (plain group), and 269 flew from Chengdu to Lhasa (3700m) in 2 hours, and then 147 of them were exposed in the high altitude for 1 day (acute high altitude exposure group), and another 122 for 7 days (short-term acclimation group). The demographic data were collected and the blood routine and platelet-associated parameters were measured of all the participants, and then the information collected were compared between the 3 groups. Results Compared to the plain group, the platelet count (PLT), platelet distribution width (PDW) and plateletcrit (PCT) reduced and the mean platelet volume (MPV) increased significantly (P<0.05) in the acute high altitude exposure group; while all the indexes in short-term acclimation group returned to approach the levels in the plain group, but statistical differences still existed in PLT, PDW and PCT (P<0.05). The platelet activating factor (PAF) and epinephrine (Epi) decreased markedly in acute high altitude exposure group than in plain group (P<0.05); while in short-term acclimation group, the PAF returned to approach the level in plain group, and the Epi was further down (P<0.05). In addition, no marked difference of 5-HT level was observed in the 3 groups (P>0.05). Pearson correlation analysis indicated that high altitude-induced reduction of oxygen saturation (SpO 2 ) was positively related to the changes of PLT. Conclusions Acute high altitude exposure may reduce PLT, PDW and PCT levels, but elevate MPV and enhance the [ ] ( ) ( ) [ ] [ ] ( ) [ ] huanglan260@126.com

2 platelet activity. The reduction of SpO 2 might be associated with the changes of PLT, PDW and PCT. Platelet-associated parameters may be recovered to normal with a compensatory effect after short-term acclimation. [Key words] high altitude; acclimation; platelet; platelet activity 2500m [1-3] [4-10] [11-14] [15] [16] [17-28] ~ ( 500m n=193) ( 500m) 2h ( 3700m) 1d( n =147) 7d( n =122) (heart rate HR) (blood pressure BP) (pulse oxygen saturation SpO 2 ) ( ) (HR BP SpO 2 ) HR BP OMRON HEM-6200 ( ) SpO 2 NONIN ONYX 9500 ( ) BC-3000 plus ( ) ELISA (5-hydroxytryptamine 5-HT) (platelet activating factor PAF) (Epinephrine Epi) ELISA 1.3 SPSS 19.0 x±s 3 LSD-t Kruskal-Wallis Nemenyi % χ 2 SpO 2 Pearson P< (body mass index BMI) (P>0.05) (systolic blood pressure SBP) (diastolic blood pressure DBP) HR SpO 2 (P<0.05 1) 2.2 (WBC) (PLT) (platelet distribution width PDW) (plateletcrit PCT) (P<0.05) (hemoglobin HGB) (RBC) (mean platelet volume MPV) (P<0.05) PLT PDW PCT (P<0.05) MPV (P<0.05)

3 Med J Chin PLA, Vol. 43, No. 3, March 1, Item 1 Tab.1 Comparison of demographic characteristics and physiological parameters between each group Plain group (n=193) Acute high altitude exposure group (n=147) Short-term acclimation group (n=122) F or χ 2 value Age (year) BMI (kg/m 2 ) Smoking [n(%)] 121(62.7) 102(69.4) 84(68.9) Drinking [n(%)] 143(74.1) 109(74.1) 88(72.1) SBP (mmhg) DBP (mmhg) HR (beat/min) SpO 2 (%) BMI. Body mass index; SBP. Systolic blood pressure; DBP. Diastolic blood pressure; HR. Heart rate; SpO 2. Oxyhemoglobin saturation P value HGB RBC (P<0.05 2) 2 (x±s) Tab.2 Comparison of platelet and blood routine parameters between each group (x±s) Item 2.3 PAF Epi (P<0.05) 5-HT (P>0.05) 5-HT PAF (P<0.05) (P>0.05) Epi 3 (P<0.05 3) 2.4 SpO 2 PLT MPV PDW PCT SpO 2 5-HT PAF Epi PLT PDW PCT SpO 2 (P<0.05) PLT MPV 5-HT PAF Epi (P>0.05 4) 3 Plain group (n=193) Acute high altitude exposure group (n=147) Short-term acclimation group (n=122) WBC (10 9 /L) (1) (1) HGB (g/l) (1) (1)(2) RBC (10 12 /L) (1) (1)(2) PLT (10 9 /L) (1) (1)(2) MPV (fl) (1) (2) PDW (%) (1) (1)(2) PCT (%) (1) (1)(2) WBC. White blood cell; HGB. Hemoglobin; RBC. Red blood cell; PLT. Platelet; MPV. Mean platelet volume; PDW. Platelet distribution width; PCT. Plateletcrit; (1)P<0.05 compared with plain group; (2) P<0.05 compared with acute high altitude exposure group 3 (ng/ml x±s) Tab.3 Platelet-associated parameters in the serum between each group (ng/ml, x±s) Item Plain group (n=193) Acute high altitude exposure group (n=147) Short-term acclimation group (n=122) 5-HT (2) PAF (1) (2) Epi (1) (1)(2) 5-HT. 5-hydroxytryptamine; PAF. Platelet activating factor; Epi. Epinephrine; (1)P<0.05 compared with plain group; (2)P<0.05 compared with acute high altitude exposure group 4 Tab.4 Pearson correlation of platelet parameters Item Correlation PLT MPV PDW PCT Correlation coefficient SpO 2 P value < <0.001 < HT PAF Correlation coefficient P value Correlation coefficient P value Correlation coefficient Epi P value PLT. Platelet; MPV. Mean platelet volume; PDW. Platelet distribution width; PCT. Plateletcrit; SpO 2. Oxyhemoglobin saturation; 5-HT. 5-hydroxytryptamine; PAF. Platelet activating factor; Epi. Epinephrine SpO 2 PLT PDW PCT WBC MPV PLT PDW PCT WBC MPV WBC PLT PDW PCT HGB RBC HGB RBC

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