Antitumor activity of an exopolysaccharide from Trichoderma pseudokoningii complexes carrying 5-fluorouracil on CT26-tumor bearing mice

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1 CT26 CT R A DOI / j. issn Antitumor activity of an exopolysaccharide from Trichoderma pseudokoningii complexes carrying 5-fluorouracil on CT26-tumor bearing mice ZHU Lei LU Jingbo CHEN Kaoshan Department of Pharmacy Wannan Medical College Wuhu China Abstract Objective To observe the antitumor effect of exopolysaccharide EPS from Trichoderma pseudokoningii containing 5-fluorouracil 5-FU in tumor bearing mice using in vivo technique. Methods CT26-tumor bearing mouse models were developed and the effects of EPS on inhibition of the tumor and physiological indexes were determined. Results Combined EPS with 5-FU significantly inhibited the CT26 tumor growth in a dose-dependent manner and the inhibition rate in CT26 tumor was higher using the complexes than simple 5-FU. Still the physiological index and the quality of life were improved in the tumor bearing mice after intervention with EPS plus 5-FU. Conclusion Combined EPS with 5-FU presents enhanced antitumor activity and EPS could attenuate toxicity effect induced by 5-FU. Key words Trichoderma pseudokoningii exopolysaccharide antitumor activity immunoregulation MH197 KJ2015A jc llz4925@ 163. com ksc313@ 126. com LI X PABLA N WEI Q et al. PKC-delta promotes renal tubular -α -1β J. cell apoptosis associated with proteinuria J. J Am Soc Nephrol NAVANEETHAN S D NIGWEKAR S U STRIPPOLI GF. Aldosterone antagonists for preventing the progression of chronic kidney disease J. Cochrane Database of Systematic Reviews QIU W ZHOU J ZHU G et al. Sublytic C5b-9 triggers glomerular mesangial cell apoptosis via XAF1 gene activation mediated by p300-dependent IRF-1 acetylation J. Cell Death Dis e CHEN KC PENG CC HSIEH CL et al. Exercise ameliorates renal cell apoptosis in chronic kidney disease by intervening in the intrinsic and the extrinsic apoptotic pathways in a rat model J. Evid Based Complement Alternat Med directly to Atg8 / LC3 to facilitate degradation of ubiquitinated protein aggregates by autophagy J. J Biol Chem J J DONG Z. Introduction autophagy in kidneys J. Semin Nephrol RUBINSTEIN AD KIMCHI A. Life in balance-a mechanistic view of the crosstalk between autophagy and apoptosis J. J Cell Sci DING WX YIN XM. Mitophagy mechanisms pathophysiological roles and analysis J. Biol Chem ROMAO S MUNZ C. LC3-associated phagocytosis J. Autophagy PANKIV S CLAUSEN TH LUMARK T et al. p62 /AQATM1 binds

2 BALB /C 4 ~ 8 18 ~ 20 g CO 2 SANYO OLYMPUS EYELA N SHB-Ⅲ S LABCONCO LABCONCO Sigma4K15 3 Sigma TDL-5-A 1. 2 polysaccharides EPS PDA r /min 7 d 4 1 /3 3 NO PBS CT /ml 12 K562 75% HL-60 SGC ml / K562 SGC EPS 5-FU CT Trichoderma pseudokoningii Sevag = 4 1 V /V D101 EPS CT26 TPS1-1 RPMI % CO 2 37 CT26 5- BALB /C 5-FU 5-FU extracelluar polysaccharides of Trichoderma pseudokoningii 200 mg / kg d + 5-FU 20 mg / kg d 20 mg / kg d EPS EPS CT26 10 d 12 h 8 5-FU 7 EPS + 5- FU 7 EPS + 5-FU % EPS + 5-FU 7 75% EPS CT % Gibco 5-FU = - Sigma RPMI 1640 / 100%

3 119 = mg / g = mg / g EPS 5-FU FU 5-FU 12 h EPS 75% 5-FU 5-FU 5-5-FU CT EPS 5-FU 5-FU 12 h 5-FU EDTA EPS 200 mg / kg d 5-FU ± 20 mg / kg d P < SPSS % EPS 5-FU CT26 Duunett-t P < FU 2 EPS 2. 1 EPS 5-FU CT CT26 x 珋 ± s / mg / kg d /g /g /% ± ± FU ± *** ± *** EPS + 5-FU 50 EPS FU ± ***# ± *** EPS + 5-FU 100 EPS FU ± **## ± *** EPS + 5-FU 200 EPS FU ± * ### ± *** F P - < < * P < **P < ***P < FU #P < ##P < ###P < CT26 x 珋 ± s / mg / kg d /g / mg /g /g / mg /g ± ± ± ± FU ± *** ± *** ± *** ± * EPS + 5-FU 50 EPS FU ± **## ± * ## ± ## ± ## EPS + 5-FU 100 EPS FU ± *** ± *** ± ** ± * EPS + 5-FU 200 EPS FU ± *** ± *** ± # ± F P - < < < * P < **P < ***P < FU #P < ##P < EPS 5-FU CT EPS 5-FU CT26 5-FU 4 P < P < FU CT26 EPS 5-FU 5-FU 5-FU EPS 5-FU EPS 5-FU 5-FU P <

4 FU EPS CT26 3 x 珋 ± s / mg / kg d /g /g /g ± ± ± FU ± ± ** ± *** EPS + 5-FU 50 EPS FU ± ± ± EPS + 5-FU 100 EPS FU ± ± ± EPS + 5-FU 200 EPS FU ± ± * ± * F P * P < **P < ***P < x 珋 ± s / mg / kg d / /L / 10 9 /L / 10 9 /L ± ± d ± d FU ± ± ± EPS + 5-FU 50 EPS FU ± ± ± * ## EPS + 5-FU 100 EPS FU ± ± ± # EPS + 5-FU 200 EPS FU ± ± ± F P * P < FU #P < ##P < IL FU 5-FU 15 5-FU EPS EPS 5-FU FU 5-FU 12 K EPS 5-FU 5-FU

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