19 14 Vol. 19 No China Journal of Modern Medicine Jul % 57.9% 70.0% % 25.2% % 5.7% 22.2%

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1 19 14 Vol. 19 No China Journal of Modern Medicine Jul , [ ] ~ % % P < % 57.9% 70.0% P < % 5.7% 25.2% % 22.2% 66.7% P < R A Clonorchis sinensis eggs involved in the formation of gallbladder stones QIAO Tie 1,2, MA Ruihong 2, ZHANG Yangde 1, FENG Yuyang 2, WANG Xingqiang 2, WANG Xiaofeng 2 (1.National Hepatobiliary and Enteric Surgery Research Center, Central South University, Changsha, Hunan, P.R.China; 2. the Second People s Hospital Panyu, Institute of Gallbladder Disease of NHEC, Institute of Gallbladder Disease, Guangzhou, Guangdong , P.R. China) Abstract: Objective To investigate the relationship between clonorchiasis infection and cholecystolithiasis. Methods In our present study, we chose 204 portions of gallbladder stones by random from 204 patients who accepted gallbladder-retention cholelithotomy during ~ at general surgery department in our hospital, and then we grinded them to explored the internal structure and construction. Meanwhile, 108 portions of homochronous bile were chose randomly, and bile sediment was obtained through centrifugalization, and then slides were smeared and tested by microscopy. Results 163 of the 204 cases of gallbladder stones were detected C. sinensis eggs in the core (79.9%), while 46 of the 108 cases of bile could be detected C. sinensis eggs (42.6%), and they had obvious statistical difference (P <0.0001). The detection rate of C. sinensis eggs was 89.6%, 57.9% and 70.0% in the pigment calculus, cholesterol and mixed calculus respectively, which had obvious statistical difference (P <0.0001). 159 of the 204 cases were from clonorchiasis-high-incidence area, such as Guangdong and Guangxi (Pigment calculus, cholesterol and mixed calculus occupied 69.2%, 5.7% and 25.2% respectively), 45 of them were from clonorchiasis-low-incidence area, such as Gansu and Sichuan (Pigment calculus, cholesterol and mixed calculus occupied 11.1%, 22.2% and 66.7% respectively.), the constituent ratio of the two groups had obvious statistical difference (P <0.0001). Conclusions Our data for the first time demonstrated that clonorchiasis infection had a close relationship with Cholecystolithiasis: we found that it was mainly pigment calculus in clonorchiasis-high-incidence area

2 14 and cholesterol and mixed calculus in clonorchiasis-low-incidence area and the detection rate of C. sinensis eggs in pigment calculus was much higher than that in cholesterol and mixed calculus; microscopic examination of gallbladder stones maybe the economical, easy and effective method for the diagnosis of clonorchiasis infection. Keywords: Pigment Calculus; Cholesterol Calculus; Mixed Calculus; Clonorchis sinensis eggs; Cholecystolithiasis SPSS11.5 χ 2 LSD x±s P <0.05 [1] ~ ~ ± >70% 1a A >70% 1b B <6 1c C 204 mm % % % CHiAO ZL >5 mm <5 mm CHiAO ZL BX ~3 0.3~1.5 cm BX r/min 10 min a A b B c C

3 μm 17μm 79.9% % 2a A % 1b B % 2c C 10% 42.6% % 100% % % 90% 10% a A b B c C ~ % [2] 78.5%~85.0% [3]

4 [4] [8] [5] [6] % % 3 P < β [9] 5.7% 69.2% 25.2% 90% 22.2% 11.1% 66.7% 90% 1 P < P < [7] % 70.0% 89.6% 2 2 P < % P <

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