Human In sulin- like Growth Factorg in M ethylotroph ic Y ea st P. p astoris
|
|
|
- Γολγοθά Σπυρόπουλος
- 5 χρόνια πριν
- Προβολές:
Transcript
1 V o l. 15,N o. 6 Ch inese Jou rnal of B iochem istry and M o lecu lar B io logy D ec., 1999 g P. p astoris 3 (, ), : 5 IGF2g ; ; Α P. p astoris, IGF2g ;, 60 m ggl P. p astoris rh IGF2g,,N g, P. p astoris,, H igh- level Express ion, Secretion and Character iza tion of Human In sulin- like Growth Factorg in M ethylotroph ic Y ea st P. p astoris L I J ingjun, HUAN G B ingren (Institu te of B asic M ed icine S ciences, CA M S & PUM C, T he N ational L aboratory of M ed ical M olecu lar B iology, B eij ing ) Abstract To increase the exp ression level of IGF2g, an exp ression vecto r con tain ing a tandem of 5 IGF2g exp ression cassettes w ith alcoho l ox idase (AOX1) p rom o ter w as con structed. T he gene encoding the m atu re fo rm of IGF2g w as fu sed in fram e to the S. cerev isiae Α2m ating facto r leader sequence, that is, the secretion of IGF2g depended on the secretion signal sequence of Α2facto ṙ A fter tran sfo rm ation of P. p astoris p ro teinase2deficien t strain w ith linearized exp ression vecto r, the tran sfo rm an ts exp ressed and secreted IGF2g to the grow th m edium w ere screened. T hen the cu ltu re of exp ression strain w as op tim ized and scaled up, the yield in the supernatan t of h igh b iom ass ferm en tation cu ltu re w as at the level of 60 m ggl. Fu rther analyses of rh IGF2g p roduced in P. p astoris show that it has co rrect M r,n 2term inal am ino acid sequence and h igh b ioactivity. Key words In su lin2like grow th facto r g ( IGF2g ), P. p astoris, H igh2level exp ression, Secretion g ( IGF2g ) Bell 1984 h IGF2g Cohn (Cohn fraction) cdna, rh IGF2g E. coli [ 1 ], 67, [ 5, 6 ], E. coli [ 7 62%, IGF2g ], H ansenu la p oly 2 [ 2 ] IGF2g IGF2g, N IH 3T 3 [ 9 ], [ 3 ], IGF2g IGF2g, [ 4 ], h IGF2g 3 T el: (010) , Fax: (010) E2m ail: huangbr@cdm. im icam s. ac. cn, [ 8 m orp ha ] 700 ggl [ 7 ],, ,, : , :
2 h, 2% P. p astoris 116 rh IGF-g M illi2 h IGF2g, po re m HA, (N H 4) 2SO 4, P. p astoris rh IGF2g Phenyl2Sepharo se Fast F low, 60 m ggl (W aters 20g20) ; SP2, rh IGF2g Sepharo se H igh, IGF2g (XK16g15), ; IGF2g IGF2g cdna, 5 Α2 (ΑF), SD S2PA GE [ 11 ], 3 c (cyct ) psk N SB 2IGF2g, pao 815 T ricine2sd S2PA GE PVD F [ 12 ], K562 A Beckm an L F 3000 AB I491 P. p astoris SM D 1168 Invitrogen, h is4p ep P rom ega T ricine, CA PS Sigm a h R &D,, HR P V ecto r P. p astoris pao 815 IGF2g Boeh ringer IGF2g S. cerev isiae Α PVD F Gelm an 0145 m HA M illipo re Α IGF 2g cdna Pharm acia 113 MM BM G BMM Invitrogen Α2F IGF2g cdna, E co R PTM 1 g B am H g pu C18, pu C182 [10 ] 114 Α - IGFg g, pu C182ΑF2IGF2g B am H g, : 5 2G GAA T TC A CCA T GA GA T T TCC K lenow E co R g T TCAA T T T T T 23 ( E cor I,, PCR 5 Α Kozak ) ; : 5 2 A T G Kozak E co R g T T TCCCA GTCA CGA CGT T P. p astoris E co R g, pu C19, pu C192Α 2IGF2g : 20 m l BM G 24 h, 500 m l BM G 18 h515 L BM G, E co R g, pao 815 E co R g ; H, N H 4OH ph 5, 500 m l, 30, 40%,, B g l g rgm in, 24 h ( B am H g 5 IGF2g ) 750 m l 50% 12 m lgl PTM 1, pao IGF2g, ph 5 315, F ig. 1, F ig. 2 pao m lgl PTM 1, 1% H it rap H igh Sub Perfo rm ance 117 P. p astoris rh IGF-g rh IGF2g [7 ] W estern T ricine2 211 pao815-5 IGF-g ( 85 ) pao 815 E cor g, E cor g B am H g psk43sb 2IGF2g ΑF2IGF2g IGF2g 3 E co R, 3 pu C18, Α, pao 8152IGF2g 5
3 6 : g P. p astoris 895 F ig. 1 Construction of the exp ression vecto r 212 rh IGF-g H is + M u t s h is4 ( ) IGF2g AOX1 ( ) [ 7, 8 ]. P. p astoris SM D 1168, IGF2g,, rh IGF2g S a l g B g l g, P. 715 kd (F ig. 3) p astoris h is4 AOX1 P. p astoris rh IGF2, S a l g g, DNA, H is + M u t +, 13%B g l g M u t + M u t s 3 4 d, 612 kd M u t + M u t s H is +, 811 kd, h IGF2g 5 AOX1 h IGF2g 3 PCR
4 896 15, 570 bp ( ), h IGF2g F ig. 3 Screening fo r the po sitive transfo rm ants of h igh2 level yield 1:M ut + SM D 1168gpAO 815; 2-4:M ut + po sitive transfo rm ants; 5:M ut s SM D 1168gpAO 815; 6-7:M ut s po sitive transfo rm ants; 8: L ow 2range p ro tein mo lecular w eigh t m arker F ig. 2 Restriction endonuclease analyses of pao IGF2 g digested w ith B g l g and B am H g (A ) and S al g (B) (A )D igested w ith B g l g and B am H g ; 1: pao IGF2g gb g l g + B am H g (2. 4 kb, 4. 0 kb, 9. 0 kb) ; 2: pao IGF2g gb g l g + B am H g (2. 4 kb, 4. 0 kb, 5. 4 kb) ; 3: pao 8152IGF2g gb g l g + B am H g (1. 8 kb, 2. 4 kb, 4. 0 kb) ; 4: pao 815gB g l g + B am H g (1. 3 kb, 2. 4 kb, 4. 0 kb) ; 5: ΚgH in d g (B)D igested w ith S al g ; 1: pao 815gS al g (7. 7 kb) ; 2: pao 8152IGF2g gs al g (8. 2 kb) ; 3: pao IGF2g gs al g (11. 7 kb) ; 4: pao IGF2g gs al g (15. 3 kb) ; 5: ΚgH in d g SD S2PA GE (F ig. 3), M u t + M u t s M u t + phy, rh IGF2g P. p astoris 1%, 3 4 d 213 hr IGF-g, 6 L ( 220 ggl ), rh IGF2g 60 m ggl H it rap, SD S2PA GE 90% (F ig. 4) F ig. 4 Purification of rh IGF2g 1: Supernatant of ferm entation culture; 2: Samp le purified by hydrophobic interaction chrom atogra2 phy and cation exchange chrom atography; 3: Samp le purified by hydrophobic interaction chrom atogra2, M u t s, 214 P. p astoris rh IGF-g, M u t
5 6 : g P. p astoris 897 F ig. 5 B ioactivity assay of P. p astoris2derived h r IGF2g g Samp le, Standard F ig. 6 W estern2blo t analysis of rh IGF2g 1: L ow 2range p ro tein mo lecular w eigh t m arker; 2: Pep tide m arker; 3: P. p astoris2derived rh IGF2g ; 4: E. coli2derived rh IGF2g ; 5: Culture supernatant of SM D 1168gpAO 815 2,, P. p astoris rh IGF2g 10 nggm l : B ip, PD I, Cy2 K562, cloph ilin s Calnex in [ 14 ],, (F ig. 5) ; P. p astoris SM D 1168gpAO 815 K562 rh IGF2g, rh IGF2g W estern T ris2 T ricine2sd S2PA GE, W estern, 13 C F ig. 6, P. p astoris rh IGF2g, 13 C IGF2g, 715 kd;, NM R rh IGF2g T ricine2sd S2PA GE P. p astoris N N 15, A YR PSETL 2, N h IGF2g 3 IGF2g, IGF2g,, IGF2g P. p astoris,,,, PAOX1,, SM D 1168, IGF2g,,, IGF2g IGF2g [ 13 ], ph,, ph 315, 1% 2% IGF2g 3, E. coli,, IGF2g SD S PA GE CGGELVD Α 2h IGF2g, P. p astoris (KEX2 ) T ricine2sd S2PA GE
6 T ricine, 5 20 kd ; 2SD S SD S, 5 20 kd, [ 11 ] References 1 R inderknech t E, H um bel R E. P rim ary structure of hum an in2 sulin2like grow th facto r2g. F EB S L ett, 1978, 89: Stew art C, Ro tw ein P. Grow th, differentiation, and survival: m ultip le physio logical functions fo r insulin2like grow th fac2 to rs. P hy siol R ev, 1996, 76: F lo rini J R, Ew ton D Z,M cw ade F J. IGF s, m uscle grow th, and m yogenesis. D iabetes R ev, 1995, 3: Feldm an E L, Sullivan K A, Kim B, Russell J W. Insulin2like grow th facto rs regulate neuronal differentiation and survival. N eu robiol D is, 1997, 4: Furm an T C, Epp J, H siung H M, Ho sk ins J,Long G I,M endel2 sohn E G, Schoner B, Sm ith D P, Sm ith M C. Recom binant hu2 m an insulin2like grow th facto r g exp ressed in E scherich ia coli. B iogt echnology, 1987, 5: Cantrell A S, Burgett S G, Cook J A, Sm ith M C, H siung H M. Effects of second2codon m utations on exp ression of the insulin2 like grow th facto r2g 2encoding gene in E scherichia coli. Gene, 1991, 98: H amm arberg B, M ok s T, T ally M, E lm blad A, Ho lm gren E, M urby M, N ilsson B, Jo seph son S, U h len M. D ifferential stabil2 ity of recom binant hum an insulin2like grow th facto r2g in E s2 cherichia coli and S tap hy lococcus aureuṡ J B iotech, 1990, 14: Faber K N,W estra S,W aterham H R, Keizer2Gunnink I, H ard2 er W, V eenhuis G AB M. Fo reign gene exp ression in H ansenula po lymo rpha. A system fo r the synthesis of sm all functional pep2 tides. A pp l M icrobiol B iotechnol, 1996, 45: Buergisser D M, Ro th B V, L ueth i C, Gerber H P, Honegger A, H um bel R E. Exp ression of a hum an insulin2like grow th facto r g cdna in N IH 23T 3 cells. B iochem B iop hy R es Comm u, 1990, 169: C lare J J, Raym ent F B, Ballantine S P, Sceek rishna K, Rom ano s M A. H igh2level exp ression of tetanus toxin fragm ent c in P ichia p astoris strains containing m ultip le tandem integrations of the gene. B iogt echnology, 1991, 9: Schagger H, Jagow G V. T ricine2sodium dodecyl sulfate2po ly2 acrylam ide gel electropho resis fo r the separation of p ro teins in the range from 1 to 100 kd a. A nal B iochem, 1987, 166: M atsudaira P. Sequence from p icomo le quantities of p ro teins electroblo tted onto po lyvinylidene difluo ride m em branes. J B iol Chem, 1987, 262: Ceuninck F D,W illeput J, Co rvo lm. Purification and characteri2 zation of insulin2like grow th facto r g ( IGF g ) and IGF g vari2 ant from hum an p lacenta. J Ch rom atog rap hy. 1995, 666: H ammod C, H elenius A. Q uanlity Contro l in the secreto ry path2 w ay. Cu rr Op in Cell B iol, 1995, 7:
cd NA ,, Expression and Character iza tion of Type-1 Pla sm inogen Activa tor Inh ibitor in P ich ia p astoris PA I21 , 1% 7 d, W estern b lo t
14 5 V o l114,n o15 1998 10 Ch inese Jou rnal of B iochem istry and M o lecu lar B io logy O cṫ 1998 g cd NA P p astoris 3 (, 200032) 379 PA I21 cdna AOX1 PHO 1, py IS21 P p astoris, H is + M u t -,, 1%
T IM P-1 cd NA CO S-7. Clon ing of Human T IM P-1 cd NA and its Expression in COS-7 Cells. 21 (tissue in2
ISSN 100727626 CN 1123870gQ 16 3 Ch in. J. B iochem. M o l. B io l. 2000, V o l. 16, N o. 3, 306 311 2000 6 T IM P-1 cd NA 3 CO S-7 3 3 (,,, 100853) GenBank T IM P21, R T 2PCR T IM P21 cdna T 2A pcr R
Con struction and B ioactiv ity of Cys57 M utan t of Human In testina l Trefo il Factor
ISSN 100727626 CN 1123870gQ 16 5 Ch in. J. B iochem. M o l. B io l. 2000, V o l. 16, N o. 5, 606 611 2000 10 57 3 (,, 100871) PCR C 57 h IT F pgex24t 21, IT PG, Glu tath ione2sepharo se 4B, Sephacryl S
Ch in. J. B iochem. M o l. B io l. 2000, V o l. 16, N o. 5, Cd 2+ : Cd 2+ ΛCd 2+
ISSN 100727626 CN 1123870gQ 16 5 Ch in. J. B iochem. M o l. B io l. 2000, V o l. 16, N o. 5, 631 636 2000 10 ΑΑ-cD NA Cd 2+ 3 (,, 100871), Β, Α Α Β, ΑΑ PCR ΑΑ2cDNA A T G AA CA, CaM V 35S ΑΑ2cDNA pgptv
Expression of Functiona l D oma in g of Ca lpa sta tin in E. coli and Prepara tion of An tiserum Aga in st Pur if ied GST-Ca lpa sta tin
ISSN 100727626 CN 1123870gQ 16 1 Ch in. J. B iochem. M o l. B io l. 2000, V o l. 16, N o. 1, 23 27 2000 2 g 3 1) 2) 2) 1) ( 1), 2), 100094) PCR (calpastatin) cdna g (404 bp ), pgex2kg pgex2calp 4 IPT G
acrysta ll iferous stra in Btk BE20
J ou rnal of N anj ing A g ricu ltu ral U niversity S18-4 - Btk BE20 3 1 2 2 2 2 ( 1, 321004; 2, 510275) S1824, PH T 3101, DNA P stl, B tk BE 20 SD S2PA GE, 2,, S1824 ; 2 ; ; S48217 Clon ing and expression
PUR IF ICATION AND CHARACTER IZATION OF EXTRACELL ULAR PROTEASE ECPASE 54 FROM A EROM ONAS H YD RO PH ILA
1 9 9 6, 1 9 ( 3 ) : 8 8 9 4 J ou rna l of N anj ing A g ricu ltu ra l U n iversity ECPase 54 Ξ 3 3 (, 210095) (A h) J21 56 (ED TA ) (PM SF), (ECPase), A h J 21 SD S2PA GE, 37,, 5 A h J21 D EA E2 Sephadex
Expression and Pur if ica tion of W ild and M utan t Type NAD H-Cytochrom e b5 Reducta se in E. coli
ISSN 100727626 CN 1123870gQ 16 4 Ch in. J. B iochem. M o l. B io l. 2000, V o l. 16, N o. 4, 452 457 2000 8 NAD H- b5 (, 350025) NADH 2 b5, (b5r ), (C203Y) b5r cdna pgex22t, BL 21 W estern GST 2b5R 2Sepharo
Effects of PD on IL -6- induced Growth Arrest and Term ina l D ifferen tia tion of M 1 Cells
ISSN 100727626 CN 1123870gQ 2001 2 Ch inese Jou rnal of B iochem istry and M o lecu lar B io logy 17 (1): 115 120 PD 098059 IL -6 M 1, 3,,, (, 100850) IL 26 M 1 R asgm A PK, M EK PD 098059 R asgm A PK,
GPS, 0. 5 kg ( In tegrated Fertility Index, IF I) 1. 1 SPSS 10. IF I =
34 11 () V o l. 34 N o. 11 2006 11 Jour. of N o rthw est Sci2T ech U niv. of A gri. and Fo r. (N aṫ Sci. Ed. ) N ov. 2006 α 1, 1, 2, 1 (1, 450002; 2, 410007) [ ]12 () 1 612,, ( IF I ) : (1), ( ) ( ), ph
) V o l. 40 N o. 6 : A : Q ,, W ard [ 1 ] (A v icenn ia m a rina) (K and elia cand el)
![) V o l. 40 N o. 6 : A : Q ,, W ard [ 1 ] (A v icenn ia m a rina) (K and elia cand el)](/thumbs/93/114276195.jpg ") V o l. 40 N o. 6 : A : Q ,, W ard [ 1 ] (A v icenn ia m a rina) (K and elia cand el)")
40 6 ( ) V o l 40 N o 6 2001 11 Jou rnal of X iam en U n iversity (N atu ral Science) N ov 2001 : 043820479 (2001) 0621350205,,,, (, 361005) :, (N R ), N R N R, N ac l ; N R : ( 0 30 ; 0 20 )N R, 30, 89
Postna ta l D evelopm en ta l Expression of Phosphoprote in s in Synaptosom e
14 1 V o l114,n o11 1998 2 Ch inese Jou rnal of B iochem istry and M o lecu lar B io logy Feb 1998 3 (, 100875),,, (PND ) 3 d 7 d 21 d,, ; PND 21d, :,,, Postna ta l D evelopm en ta l Expression of Phosphoprote
(g ) A quanau tics,, , 33. 1%. O , O CH EM ICAL JOU RNAL O F CH IN ESE UN IV ERS IT IES (,,, )
V o l. 19 N o. 10 1 9 9 8 10 CH EM ICAL JOU RNAL O F CH IN ESE UN IV ERS IT IES 1584 1588 (g ) (,,, 300071) (, ),,..,, 1. 0 g1,., 33. 1%.,,, O 632. 5, O 629. 7. (H b),., Bonaven tu ra 80, H b, H b 50 100
D NA , ) SCGE DNA.
14 5 V o l114,n o15 1998 10 Ch inese Jou rnal of B iochem istry and M o lecu lar B io logy O cṫ 1998 SCGE D NA (, (, 030006) 030006) (SCGE) ; SCGE Χ DNA, Χ DNA, :,DNA,, Pr inc iple and M ethod of the Single
Pur if ica tion and Character iza tion of Ang ioten sin Converting Enzym e 3
ISSN 10072762 CN 1123870gQ 16 6 Ch in. J. B iochem. M o l. B io l. 2000 V o l. 16 N o. 6 788 792 2000 12 3 3 3 ( 100037) (angio ten sin converting enzym e A CE) A CE 1. 6 2. 6 m o lgl 200 g 0. 79 m g A
F ig. 1 Flow chart of comprehen sive design in the research of com patibil ity. E2m ail: ac. cn
V o l. 25 N o. 9 2 0 0 4 9 CH EM ICAL JOU RNAL O F CH IN ESE UN IV ERS IT IES 1632 1636,,,, (, 116011) H PL C2PAD 2M S,,.,. H PL C2PAD 2M S.,.,,. 2 ; ; O 652. 7 A 025120790 (2004) 0921632205,,., [1 5 ]
(A ntifreeze p roteins, A FP s) (afp ),
29 1 ( )V ol. 29 N o. 1 2001 2 Jouṙ of N orthw est Sci2Tech U niv. of A gri. and Foṙ (N aṫ Sci. Ed. ) Feb. 2001 α 1, 1, 2, 3 (1, 712100; 2, 710032; 3, 712100) []A utum n K ing, CTAB DNA, PCR (Polym erase
Study on the M ethod of the Qua lita tive Ana lysis of Phosphoroth ioa te O ligonucleotide Can tide
ISSN 100727626 CN 1123870gQ 2001 4 Ch inese Jou rnal of B iochem istry and M o lecu lar B io logy 17 (2): 250 254 3,, (, 100850) (A CTCA CTCA GGCCTCA GA CT ),,,,,,,,,,,,, R 917 Study on the M ethod of
( , ,
33 9 ( ) V o l. 33 N o. 9 2005 9 Jour. of N o rthw est Sci2T ech U niv. of A gri. and Fo r. (N aṫ Sci. Ed. ) Sep. 2005 Ξ 1, 2, 1, 1, 1, 1, 1, 2 (1 010018; 2 512005) [],, 8 8 9, () (),,,,,, ;, ;,, ;,,,
Investiga tion on L ipoprote in (a) Receptor in the M em brane of M onkey L iver Cells
ISSN 100727626 CN 1123870gQ 16 1 Ch in J B iochem M o l B io l 2000, V o l 16, N o 1, 96 100 2000 2 (a) 3 (, 310009; 3, 030001) SD S2PA GE, LDL LDL L p (a) PL G 3, 370 kd 290 kd 80 kd, L p (a) + PL G L
(P seudo rab ies, PR ) (A u jeszky, s D isease, AD ), (Pesuderab ies viru s, PRV )
34 9 ( ) V o l. 34 N o. 9 2006 9 Jour. of N o rthw est Sci2T ech U niv. of A gri. and Fo r. (N aṫ Sci. Ed. ) Sep. 2006 W G Ξ 1, 1, 1, 1, 1, 2 (1, 712100; 2, 723600) [ ]ge2el ISA 6 187 (P seu2 do rabies
26 3 V o l. 26 N o A cta Eco logiae A n im alis Dom astici M ay ,
26 3 V o l. 26 N o. 3 2005 5 A cta Eco logiae A n im alis Dom astici M ay 2005,,,,, (, 524088) [ ] 1 200, 3,, 28 31, 83 87%, 1 2 0. 1% 0. 3% : 1 38 47 (P < 0. 05), 48 57 1 (P < 0. 05),,, 1 (P > 0. 05)
Ch in. J. B iochem. M o l. B io l. 2000, V o l. 16, N o. 6,
ISSN 100727626 CN 1123870gQ 16 6 Ch in. J. B iochem. M o l. B io l. 2000, V o l. 16, N o. 6, 814 819 2000 12 3 RNA 3 3 (, 200032) upa R RNA pu RA S M DA 2M B 2231, G418 N o rthern upa R RNA, R T 2PCR upa
Use of Site-D irected M utagenesis to Investiga te the Properties of Recom binan t H irud in-hv2
14 1 V o l114,n o11 1998 2 Ch inese Jou rnal of B iochem istry and M o lecu lar B io logy Feb 1998 (, 100101),,, rhv 2 47 11 rhv 2 Gln11 A sn47 H is11 L ys47, rhv 22H 11 30%, rhv 22K47 61% K i, rhv 22
The Targeted Express ion of Human IL -2gIFNΑ2b Fused Gene in Hepa toma Cells
15 4 1999 8 V o l 15,N o 4 Ch inese Jou rnal of B iochem istry and M o lecu lar B io logy A ug, 1999 3 IL -2gIFNΑ2b (, 200032), DNA IL 22 IFN Α, A FP galb, EA FP2PALB A, IL 22gIFN Α2b A FP IL 22 IFN IL
www.absolualarme.com met la disposition du public, via www.docalarme.com, de la documentation technique dont les rιfιrences, marques et logos, sont
w. ww lua so ab me lar m.co t me la sit po dis ion du c, bli pu via lar ca do w. ww me.co m, de la ion nta t do cu me on t ed hn iqu tec les en ce s, rι fιr ma rq ue se t lo go s, so nt la pr op riι tι
T. Kaw akam i. B ru ton. , Sf9
14 3 V o l114,n o13 1998 6 Ch inese Jou rnal of B iochem istry and M o lecu lar B io logy Jun 1998 Bruton T Kaw akam i (L a Jo lla Institute fo r A llergy and Imm uno logy, San D iego, CA, U SA ) ( ;,
Quan tif ica tion of Polym era se Cha in Reaction Products Using Solid Pha se Hybr id iza tion-enzym e Color im etr ic D etection
14 2 V o l114,n o12 1998 4 Ch inese Jou rnal of B iochem istry and M o lecu lar B io logy A p ṙ 1998 3 - (, 100044),,,, 125 g, :,,, Quan tif ica tion of Polym era se Cha in Reaction Products Using Solid
Το άτομο του Υδρογόνου
Το άτομο του Υδρογόνου Δυναμικό Coulomb Εξίσωση Schrödinger h e (, r, ) (, r, ) E (, r, ) m ψ θφ r ψ θφ = ψ θφ Συνθήκες ψ(, r θφ, ) = πεπερασμένη ψ( r ) = 0 ψ(, r θφ, ) =ψ(, r θφ+, ) π Επιτρεπτές ενέργειες
Further Study on the Spon taneous Fold ing of Ch icken Apocytochrom e c
15 1 1999 2 V o l 15,N o 1 Ch inese Jou rnal of B iochem istry and M o lecu lar B io logy Feb 1999 3 c 3 3 (, 100101) c V 92A 17, V 92A gc17s W gc17s IA EDAN S A EDAN S2Cys214 A EDAN S T rp 259 V 92A c
, ( , (P anax qu inquef olius) ) ; L C2V P series U H PE), M Pa R E252. C18 (5 Λm, 250 mm 4. 6 mm ) ( )
150 21 5 2005 5 T ransactions of the CSA E V o l. 21 N o. 5 M ay 2005,,,,, (, 130025) :,, CO 2 : 70%, 200M Pa, 50 1, 2 m in, : ; ; ; : O 62335; TQ 036 +. 4 : A : 100226819 (2005) 0520150205,,,. [J ].,
6 cm, 1. 2 IAA, NAA, 2. 1
30 6 ( ) V o l. 30 N o. 6 2002 12 Jour. of N o rthw est Sci2T ech U niv. of A gri. and Fo r. (N aṫ Sci. Ed. ) D ec. 2002 Ξ 1, 2, 2, 3, 1, 4, 1 (1 ; 2 ; 3, 712100; 4, 733200) [],,,,,, ; 1g2 M S, 1g3M S
1999, 17 (1): J ourna l of W uhan B otan ica l Resea rch ( ) ( ) 2, 3. (Celosia cristata L. ),
1999, 17 (1): 15 20 J ourna l of W uhan B otan ica l Resea rch Ξ ( 210013) (210095),, W HO g FAO, 3, 10, 3 ( ) 2317% 2714%,, 83147% 86194%, (EAA ) 4012% 4117%, (M et+ Cys) 10,,,,,,,,,,, 1 (Celosia cristata
(2006) , ; (M OD S) (VA P) ,
(2006) 65 Practical guidelines for m echan ical ven tilation (2006) S ociety of C ritical Care M ed icine Ch inese M ed ical A ssociation Corresp ond ing au thors: Q IN Y ing 2z h i (D ep artm ent of Intensive
IL -4 . IL 24, cp IL 42PE38KD EL,.
V o l. 26 N o. 5 2 0 0 5 5 CH EM ICAL JOU RNAL O F CH IN ESE UN IV ERS IT IES 874 879 IL -4 1, 2, 3, 1, 2, 1, 2 (1., 110016; 2., 100039; 3. Beckm an R esearch Institute, C ity of Hope N ationalm edical
TR IBOLO GY N ov, 2004 , P I2. , 120 M Pa, P I. 6 mm 7 mm 30 mm [7, 8 ] mm,. JSM 25600LV 1. 1 (EDXA ).
![TR IBOLO GY N ov, 2004 , P I2. , 120 M Pa, P I. 6 mm 7 mm 30 mm [7, 8 ] mm,. JSM 25600LV 1. 1 (EDXA ).](/thumbs/50/26571605.jpg "TR IBOLO GY N ov, 2004 , P I2. , 120 M Pa, P I. 6 mm 7 mm 30 mm [7, 8 ] mm,. JSM 25600LV 1. 1 (EDXA ).")
24 6 V o l 24, N o 6 2004 11 TR IBOLO GY N ov, 2004,,, (, 730000) : MM 2200 (PT FE) M os2 (P I) GC r15, X P I, PT FE M os2 P I, P I+ 30%M os2, P I 50%. P I+ 10% PT FE+ 20%M os2, P I, P I+ 20% PT FE+ 10%M
(H yaluronic acid, HA ) , HA,. HA,, HA. N T G (S trep tococcus zooep id em icus J 18), 50 g
V ol. 25 N o. 5 2 0 0 4 5 CHEM ICAL JOU RNAL O F CH IN ESE UN IV ERS IT IES 853 857 1, 1, 1, 1, 2, 1 (1., 130023; 2., 130022) N 2 2N 2 2N 2 (N TG),., Savage D EA E2 (D E52) Sephadex G275., (S trep tococcus
M olecular Clon ing of D eletion Br idge Fragm en t of Y unnanese
15 1 1999 2 V o l 15,N o 1 Ch inese Jou rnal of B iochem istry and M o lecu lar B io logy Feb 1999 Y unnanese ( A Χ Β) 0-3 (,,, 100005) Yunnanese ( A Χ Β) 0 2, 3, EM BL 3, 11 G Χ 617 kb 1115 kb 3 ( ) 1115
(M u ltidrug resistance, M DR )
V o l. 26 N o. 8 2 0 0 5 8 CH EM ICAL JOU RNAL O F CH IN ESE UN IV ERS IT IES 1446 1450 1, 1, 2 (1., 132013; 2., 130023) V CR Bel27402 M DR,.,, V CR. Bel27402, V CR, V CR G2, P2170, M DR 1gmRNA, Topo gαmrna.
(T rip tery g ium w ilf ord ii Hook) ,Beroza [6 ] 4 W ilfo rine W ilfo rdine W ilfo rgine W il2. Euon ine 1. 0% 1980, 1. 1 ; 1. 0%, ; 0.
![(T rip tery g ium w ilf ord ii Hook) ,Beroza [6 ] 4 W ilfo rine W ilfo rdine W ilfo rgine W il2. Euon ine 1. 0% 1980, 1. 1 ; 1. 0%, ; 0.](/thumbs/92/108790990.jpg "(T rip tery g ium w ilf ord ii Hook) ,Beroza [6 ] 4 W ilfo rine W ilfo rdine W ilfo rgine W il2. Euon ine 1. 0% 1980, 1. 1 ; 1. 0%, ; 0.")
34 12 ( ) V o l. 34 N o. 12 2006 12 Jour. of N o rthw est Sci2T ech U niv. of A gri. and Fo r. (N aṫ Sci. Ed. ) D ec. 2006 Ξ 1, 3, 1, 2, 1, 2, 1, 1, 2 (1, 712100; 2, 712100; 3, 450008) [ ], 0. 1%, 3 48
Ch in. J. B iochem. M o l. B io l. 2000, V o l. 16, N o. 2, K 3 (A 6D )
ISSN 100727626 CN 1123870gQ 16 2 Ch in. J. B iochem. M o l. B io l. 2000, V o l. 16, N o. 2, 234239 2000 4 (P I4-K) 3 1) (,, 100101; 1), 100871) (P I 42K) (A 6D ) 2, 71510 6 610 8 (m o lgl ) - 1 11910-7
Appendix B Table of Radionuclides Γ Container 1 Posting Level cm per (mci) mci
3 H 12.35 Y β Low 80 1 - - Betas: 19 (100%) 11 C 20.38 M β+, EC Low 400 1 5.97 13.7 13 N 9.97 M β+ Low 1 5.97 13.7 Positrons: 960 (99.7%) Gaas: 511 (199.5%) Positrons: 1,199 (99.8%) Gaas: 511 (199.6%)
(H ipp op hae rham noid es L. ) , ; SHB 2g , 1. 0, 2. 0, 3. 0, 4. 0, 5. 0 ml mm. : Y = X - 0.
34 10 () V o l. 34 N o. 10 2006 10 Jour. of N o rthw est Sci2T ech U niv. of gri. and Fo r. (N aṫ Sci. Ed. ) O cṫ 2006 Ξ 1, 2, 1, 2, 3, 2, 2 (1, 712081; 2, 712100; 3, 123000) [ ],,, : V ( ) V ( 95% ) =
A (GPA ), (Perco ll) A, Gene Express ion of Erythrocyte M em brane Glycophor in A in N IDDM
14 5 V o l114,n o15 1998 10 Ch inese Jou rnal of B iochem istry and M o lecu lar B io logy O cṫ 1998 g GPA 3 (, 410078) g (N IDDM ) A (GPA ), (Perco ll) 15 25 N IDDM, RNA, N o rthern GPA GP W estern, N
Ch in. J. B iochem. M o l. B io l. 2000, V o l. 16, N o. 3, U CP. (P < 0101) ; U CP 2 m RNA , R T 2PCR
ISSN 100727626 CN 1123870gQ 16 3 Ch in. J. B iochem. M o l. B io l. 2000, V o l. 16, N o. 3, 394 399 2000 6 3-1 2 3 3 3 (,, 100730) 21 2 3 (U CP1, 2, 3), R T 2PCR U CP 1, 2, 3 m RNA U CP 1. U CP 2, 3,
Νόµοςπεριοδικότητας του Moseley:Η χηµική συµπεριφορά (οι ιδιότητες) των στοιχείων είναι περιοδική συνάρτηση του ατοµικού τους αριθµού.
Νόµοςπεριοδικότητας του Moseley:Η χηµική συµπεριφορά (οι ιδιότητες) των στοιχείων είναι περιοδική συνάρτηση του ατοµικού τους αριθµού. Περιοδικός πίνακας: α. Είναι µια ταξινόµηση των στοιχείων κατά αύξοντα
:,UV IKON 810, 700 nm. DU 27Spectropho to neter 1. 1 U S2KT P
31 3 ( ) V o l. 31 N o. 3 2003 6 Jour. of N o rthw est Sci2T ech U niv. of A gri. and Fo r. (N aṫ Sci. Ed. ) June 2003 Ξ,,,, (, 712100) [],, 100, 217,, 18, 8,, 36. 34, 1. 32, 2. 4 : 1 15 45 2 h, 2 10 70
Stud ies on Chem ica l Con stituen ts and M orpholog ica l Characters of the Fruits of Seven Labia tae Plan ts
2003, 21 (3): 276 280 J ourna l of W uhan B otan ica l Resea rch 7 1, 2, 1 1, Ξ (1., 361005; 2., 350007) : ; ; ; ; ; : Q 946; Q 949. 777. 6 : A : 10002470X (2003) 0320276205 Stud ies on Chem ica l Con
: H IV. Screen ing of 1F7 Id iotyp ic An ti-gp160 An tibod ies from Phage An tibody L ibrary
14 1 V o l114,n o11 1998 2 Ch inese Jou rnal of B iochem istry and M o lecu lar B io logy Feb 1998 H IV-1 gp 160 2) 1) 1) 1) 3) (, 100850; 2), 100071; 3), 130062) : H IV m RNA, IgG Fd Fab, pcom b3, XL
nested touch down PCR
33 2 () V o l. 33 N o. 2 2005 2 Jour. of N o rthw est Sci2T ech U niv. of A gri. and Fo r. (N aṫ Sci. Ed. ) Feb. 2005 nested touch down PCR CAPN1 EST Ξ 1, 2, 3, 2, 4, 3 (1, 712100; 2, 730070; 3, 100083;
Ι ΙΟΤΗΤΕΣ ΤΩΝ ΑΤΟΜΩΝ. Παππάς Χρήστος Επίκουρος Καθηγητής
ΗΛΕΚΤΡΟΝΙΚΗ ΟΜΗ ΚΑΙ Ι ΙΟΤΗΤΕΣ ΤΩΝ ΑΤΟΜΩΝ Παππάς Χρήστος Επίκουρος Καθηγητής ΤΟ ΜΕΓΕΘΟΣ ΤΩΝ ΑΤΟΜΩΝ Ατομική ακτίνα (r) : ½ της απόστασης μεταξύ δύο ομοιοπυρηνικών ατόμων, ενωμένων με απλό ομοιοπολικό δεσμό.
Ch inese Jou rnal of B iochem istry and M o lecu lar B io logy. (o steoarth ritis, OA )
ISSN 100727626 CN 1123870gQ 2001 4 Ch inese Jou rnal of B iochem istry and M o lecu lar B io logy 17 (2): 244 249 1) 1), 3, 2), 1), 1), 1), 2) 2), ( 1), 710032; 2), 100044) ( rheum ato id arth ritis, RA
China Academic Journal Electronic Publishing House. All rights reserved. O ct., 2005
23 10( 142) V ol. 23, N o. 10 200510 System s Engineering O ct., 2005 100124098 (2005) 1020081205 Ξ 1, 2, 1 2, (1., 710049; 21, 266033),,,,,,,, ; ; F224. 32 A 20 6070 [1, 2 ], 1, 1. 1, (1) T = {1, 2},
(classical sw ine fever, CSF) (CSFV ) (O IE) A 15, 1999202212 96,, C2,, 20 70 80, 1. 1 SXZZ), 1. 2
31 5 () V o l. 31 N o. 5 2003 10 Jour. of N o rthw est Sci2T ech U niv. of A gri. and Fo r. (N aṫ Sci. Ed. ) O cṫ 2003 E2 gp55 Ξ 1, 1, 1, 1, 1, 2, 2 (1, 712100; 2, 730046) [ ] R T 2PCR PCR E 2,, A lfo
V o l122, N o13 M ay, 2003 PRO GR ESS IN GEO GRA PH Y : (2003) , : TU 984. , (Eco logy fo r evil) [1 ] (R ich Boyer), 2.
![V o l122, N o13 M ay, 2003 PRO GR ESS IN GEO GRA PH Y : (2003) , : TU 984. , (Eco logy fo r evil) [1 ] (R ich Boyer), 2.](/thumbs/93/114354980.jpg "V o l122, N o13 M ay, 2003 PRO GR ESS IN GEO GRA PH Y : (2003) , : TU 984. , (Eco logy fo r evil) [1 ] (R ich Boyer), 2.")
22 3 2003 5 PRO GR ESS IN GEO GRA PH Y V o l122, N o13 M ay, 2003 : 100726301 (2003) 03203162010, (, 710061) :, (R ich Boyer), 2001,, : ; ; ; ; : TU 984 1,,, (Eco logy fo r evil) [1 ], ( ), (D avid1 Sm
V irus ide ntifica tion a nd sc re e ning v irus ic ide s of pe ppe r v irus d ise a se in S ha a nxi P rov ince
35 1 () V o l. 35 N o. 1 2007 1 Journal of N o rthw est A & F U niversity (N aṫ Sci. Ed. ) Jan. 2007 Ξ 1, 2, 2, 1, 1 (1, 712100; 2, 710003) [] 126, 5, DA S2EL ISA,, (CM V ) (TM V ) (T EV ) Y (PV Y) (BBWV
FXR1 FM R 1 12 14 15 6 FM R P FXR 1. In teraction s between Six FM RP Isoform s and FXR1 Prote in
14 4 V o l114,n o14 1998 8 Ch inese Jou rnal of B iochem istry and M o lecu lar B io logy A ug 1998 FM RP 3 6 FXR1 A Sittler 3 3 J2L M andel 3 3 (, 100005; 3 3 IGBM C, CN RS2IN SERM 2UL P, 67404 Illk irch,
Bank A ssetgl iab ility Sheet w ith Em bedded Op tion s
00 8 8 : 10006788 (00) 08005506, (, 710049) :,, ;, ; : ; ; ; ; ; : F830 : A α Con tro l O ver In terest R ate R isk of Bank A ssetgl iab ility Sheet w ith Em bedded Op tion s LU O D aw ei, W AN D ifang
ΠΕΡΙΟΔΙΚΟΣ ΠΙΝΑΚΑΣ ΣΤΟΙΧΕΙΩΝ
ΠΕΡΙΟΔΙΚΟΣ ΠΙΝΑΚΑΣ ΣΤΟΙΧΕΙΩΝ Περίοδοι περιοδικού πίνακα Ο περιοδικός πίνακας αποτελείται από 7 περιόδους. Ο αριθμός των στοιχείων που περιλαμβάνει κάθε περίοδος δεν είναι σταθερός, δηλ. η περιοδικότητα
1998, 18 (1): 1 7. A cta T heriolog ica S inica (,, ) (Grow th layer group s, GL Gs) 168, 42,
1998, 18 (1): 17 A cta T heriolog ica S inica Ξ (,, 210097), 3,, 3 C (O dum, 1983), 0 2, 24 11 3 R 0 Κ1, rm 0,, ; ; ; ;, (Sh i2 rak ihara, 1993; Gao, 1993)Gao (1993) 3 56,, (, 1995),, 3,, 1974 229, 86,
ZrO 2, ZrO 2. (M ) W O 3g ( 100% ) SO 4 2- W O 3 M oo 3, SO ( Zr (OH ) 4 ) ( ( 0. 1 mo l. L - 1 A gno 3 ), Zr (OH ) 4 SO (0.
14 2 V o l. 14,N o. 2 2000 4 JOU RNAL O F M OL ECULA R CA TAL YS IS (CH INA ) A p r. 2000 : 100123555 (2000) 0220111208 WO 3gZrO 2 - g. ZrO 2,,, 1) (, 130022) : ZrO 2 WO 3gZrO 2 SO 4 2- gzro 2 M oo 3gZrO
T he Op tim al L PM Po rtfo lio M odel of H arlow s and Its So lving M ethod
2003 6 6 00026788 (2003) 0620042206 H arlow, 2 3, (., 70049; 2., 7006; 3., 200433) H arlow,,,,, ;, ; ; F832. 5; F830. 9 A T he Op tim al L PM Po rtfo lio M odel of H arlow s ad Its So lvig M ethod W AN
H IV-1 gp160. Ana lysis and Expression of An ti-h IV-1 Id iotyp ic An tibody. H IV 21 gp 160. , 1) Fab. gp 160
14 4 V o l114,n o14 1998 8 Ch inese Jou rnal of B iochem istry and M o lecu lar B io logy A ug 1998 H IV-1 gp160 1) 2) (, 100850) ) 1F7 H IV 21 gp 160 3B Fab, 1D Fd Fd, V H V Hg, 3B ϑ Κ Fab 3 3B H IV 21
ΝΟΜΟΣ ΤΗΣ ΠΕΡΙΟ ΙΚΟΤΗΤΑΣ : Οι ιδιότητες των χηµικών στοιχείων είναι περιοδική συνάρτηση του ατοµικού τους αριθµού.
1. Ο ΠΕΡΙΟ ΙΚΟΣ ΠΙΝΑΚΑΣ Οι άνθρωποι από την φύση τους θέλουν να πετυχαίνουν σπουδαία αποτελέσµατα καταναλώνοντας το λιγότερο δυνατό κόπο και χρόνο. Για το σκοπό αυτό προσπαθούν να οµαδοποιούν τα πράγµατα
N-acetyl- l-cyste ine im proves function of islet Β cell in hyperl ip idem ic rats and its m echan ism. V o l 36 N o , NA C PCR
36 6 2007 ( ) JOU RNAL O F ZH EJ IAN G UN IV ERS IT Y (M ED ICAL SC IEN CES) V o l 36 N o 6 2007 h ttp: www. journals. zju. edu. cngm ed Β 1, 1, 1, 1, 1, 1, 2 ( 1. 2., 100029) [ ] : 2N 2 (N 2acetyl2l2cysteine,
SUPPLEMENTAL INFORMATION. Fully Automated Total Metals and Chromium Speciation Single Platform Introduction System for ICP-MS
Electronic Supplementary Material (ESI) for Journal of Analytical Atomic Spectrometry. This journal is The Royal Society of Chemistry 2018 SUPPLEMENTAL INFORMATION Fully Automated Total Metals and Chromium
G IS N N E E, , km 2, 92% A CTA GEO GRA PH ICA S IN ICA
55 4 2000 7 A CTA GEO GRA PH ICA S IN ICA V o l. 55, N o. 4 July, 2000 : 037525444 (2000) 0420407210 G IS,, (, 100101) : G IS, 1982 1997 ;, : ; ; ; G IS; : F301124; F29312; N 94511 : A 20 80 90, [1, 2
Pur if ica tion and Partia l Character iza tion of Hem orrhag ic Tox in from the Venom of D ienagk istrodon acu tus
15 1999 10 V o l 15,N o 5 Ch inese Jou rnal of B iochem istry and M o lecu lar B io logy O ct, 1999 DaHT- (, 65022) (, 650091), Sephadex G275, D EA E2Sephadex A 250, Sephadex G2200 PBE, (D ienag k istrod
THE EFFECTS OF SEVERAL 5-HT RECEPTOR ANTAGON ISTS ON THE MOTOR ACTIV ITY IN SCH ISTOSOM A J A PON ICUM
1997, 20 (4): 8 87 J ou ra l of N aj ig A g ricu ltu ra l U iversity 52 α (, 210095) 52 (52H T ) : (1) 10 - mo lgl 10-4 mo lgl 52H T 0 m i,, 10-5 mo lgl, 10 - mo lgl (tryp tam ie), 10-4 mo lgl 10-5 mo
Stem Character istics of W heat w ith Stem L odging and Effects of L odging on Gra in Y ield and Qual ity
2006, 26 (1): 87 92 Journal of T riticeae C rop s Ξ,,,,, ( g, 225009) : 6,,,,,,, : ; ; ; ; : S 512. 1; S 318 : A : 100921041 (2006) 0120087206 Stem Character istics of W heat w ith Stem L odging and Effects
ΓΗ ΚΑΙ ΣΥΜΠΑΝ. Εικόνα 1. Φωτογραφία του γαλαξία μας (από αρχείο της NASA)
ΓΗ ΚΑΙ ΣΥΜΠΑΝ Φύση του σύμπαντος Η γη είναι μία μονάδα μέσα στο ηλιακό μας σύστημα, το οποίο αποτελείται από τον ήλιο, τους πλανήτες μαζί με τους δορυφόρους τους, τους κομήτες, τα αστεροειδή και τους μετεωρίτες.
F ingerpr in ts of X in shu Ora l L iquid by HPCE
839 1, 1, 2, 2 1Ξ, (11, 110015; 21, 110016) : 10, 10, 10, 10,,,,,,, 27 14, 10 ( 7 ), 9 : ; ; : R 283; R 286102 : A : 0253 2670 (2006) 06 0839 05 F ingerpr in ts of X in shu Ora l L iquid by HPCE GUO T
STUD IES ON THE GENOTOX IC ITY OF 83- ITS M AJOR M ETABOL ITE 1 HERB IC ID E AND. H eng Zhengchang 1, T O ng 2
1997 9 3 Carcinogenesis T eratogenesis and M utagenesis V o l 9 N o 3 1997 831 1 T O ng 2 1 610041 2 D ivision of R esp irato ry D isease Studies,N IO SH,U SA Am es V 79 H PR T (SCE) 831 ( ) 2, 4 6 (DCA
Fo recasting Stock M arket Q uo tation s via Fuzzy N eu ral N etw o rk Based on T 2S M odel
2001 2 2 : 100026788 (2001) 0220066207 T 2S,, (, 400044) : T 2S,,, ( ),,. : ; ; α Fo recasting Stock M arket Q uo tation s via Fuzzy N eu ral N etw o rk Based on T 2S M odel CH EN X ing, M EN G W ei2dong,
Parts Manual. Trio Mobile Surgery Platform. Model 1033
Trio Mobile Surgery Platform Model 1033 Parts Manual For parts or technical assistance: Pour pièces de service ou assistance technique : Für Teile oder technische Unterstützung Anruf: Voor delen of technische
Estimation of grain boundary segregation enthalpy and its role in stable nanocrystalline alloy design
Supplemental Material for Estimation of grain boundary segregation enthalpy and its role in stable nanocrystalline alloy design By H. A. Murdoch and C.A. Schuh Miedema model RKM model ΔH mix ΔH seg ΔH
LfcinB. Expression and Iden tif ica tion of Lfc inb Gene in P ich ia pastoris
265 ( LfcinB) 3 ( 518057) : ( Pichia pastors) ( bovine lactoferricin, Lf2 cinb), LfcinB pp IC9K, pp IC9K2LfcinB Sal, SMD1168 G418,, PCR LfcinB LfcinB, : (LfcinB),,, : Q782, Q784, Q786 : A : 025322654 (2007)
τροχιακά Η στιβάδα καθορίζεται από τον κύριο κβαντικό αριθµό (n) Η υποστιβάδα καθορίζεται από τους δύο πρώτους κβαντικούς αριθµούς (n, l)
ΑΤΟΜΙΚΑ ΤΡΟΧΙΑΚΑ Σχέση κβαντικών αριθµών µε στιβάδες υποστιβάδες - τροχιακά Η στιβάδα καθορίζεται από τον κύριο κβαντικό αριθµό (n) Η υποστιβάδα καθορίζεται από τους δύο πρώτους κβαντικούς αριθµούς (n,
Ch inese Jou rnal of B iochem istry and M o lecu lar B io logy. E, m RNA T IR
ISSN 100727626 CN 1123870gQ 2001 2 Ch inese Jou rnal of B iochem istry and M o lecu lar B io logy 17 (1): 29 34 m RNA 5 3,, (, 200031) 21 bp B 50 PCNA 2L ac Z m RNA SD 11 bp H ind g, B 50il B 50i2 m RNA,B
DY NAM IC CHANGE OF EP ID ERM AL STEM CELL S IN THE WOUND HEAL ING COURSE OF D IABETIC RATSg
693 (diabetes m ellitus, DM ) (ep iderm al stem cells, ESC s), ESC s DM 48 W istar,, 180 200 g DM, 24 DM DM 115 cm 118 cm 2154 cm 2 ( 96 ), 3 7 14 21, ;, H E 19 (keratin 19, K19) Β1, 3 7 14 21, 24148%
UPTAKE OF CAROTENO ID S BY INTESTINAL M UCOUS CELL S OF LAY ERS AND GOATS IN V ITRO
1997, 20 (4): 54 59 J ou rna l of N anj ing A g ricu ltu ra l U n iversity Ξ (, 210095) (Β2 ) : Β2, Β2 (P > 0. 05);, ( 15 Λmo lgl ) Β2 ; Tw een 20, ; (Υ) 013% Tw een 20 Β2 A ; 015 mo lgl Β2 (P < 0. 01)
C 1 D 1. AB = a, AD = b, AA1 = c. a, b, c : (1) AC 1 ; : (1) AB + BC + CC1, AC 1 = BC = AD, CC1 = AA 1, AC 1 = a + b + c. (2) BD 1 = BD + DD 1,
1 1., BD 1 B 1 1 D 1, E F B 1 D 1. B = a, D = b, 1 = c. a, b, c : (1) 1 ; () BD 1 ; () F; D 1 F 1 (4) EF. : (1) B = D, D c b 1 E a B 1 1 = 1, B1 1 = B + B + 1, 1 = a + b + c. () BD 1 = BD + DD 1, BD =
copula, 5 3 Copula Κ L = lim System s Engineering M ay., 2006 : (2006) ,,, copula Ξ A rch im edean copula (Joe,
24 5 ( 149 ) V ol. 24, N o. 5 2006 5 System s Engineering M ay., 2006 : 100124098 (2006) 0520088205 copula Ξ, (, 230052) : (copula), A rch im edean copula, Gum bel2hougard copula,, : Copula; ; : F830 :
ΠΕΡΙΟΔΙΚΟ ΣΥΣΤΗΜΑ ΤΩΝ ΣΤΟΙΧΕΙΩΝ (1) Ηλία Σκαλτσά ΠΕ ο Γυμνάσιο Αγ. Παρασκευής
ΠΕΡΙΟΔΙΚΟ ΣΥΣΤΗΜΑ ΤΩΝ ΣΤΟΙΧΕΙΩΝ (1) Ηλία Σκαλτσά ΠΕ04.01 5 ο Γυμνάσιο Αγ. Παρασκευής Όπως συμβαίνει στη φύση έτσι και ο άνθρωπος θέλει να πετυχαίνει σπουδαία αποτελέσματα καταναλώνοντας το λιγότερο δυνατό
A ( SPA ) Su lfo succin im idyl 62[ 3 2( 22pyridyldith io ) p rop ionam ido ]hexanoate (Su lfo2l C2SPD P) C E2m ail: w
![A ( SPA ) Su lfo succin im idyl 62[ 3 2( 22pyridyldith io ) p rop ionam ido ]hexanoate (Su lfo2l C2SPD P) C E2m ail: w](/thumbs/91/105112871.jpg "A ( SPA ) Su lfo succin im idyl 62[ 3 2( 22pyridyldith io ) p rop ionam ido ]hexanoate (Su lfo2l C2SPD P) C E2m ail: w")
V o l. 25 N o. 1 2 0 0 4 1 CH EM ICAL JOU RNAL O F CH IN ESE UN IV ERS IT IES 44 49 -C 1, 1, 1, 1, 2, 3 (1., 400038; 2., 400032; 3. 26, 400060) A T 10M H z, C, 2 5 2C.. 215 16010 m IU gl C 01375 1210 nggml,
Jou rnal of M athem atical Study
38 4 2005 12 Jou rnal of M athem atical Study V ol 38 o 4 D ec 2005 α (, 361005),, (SVV ), SVV, SVV, SVV - H elm holtz, (SEM ); (SVV ); O 174 52 A 1,, 1g, ( ),, 80,, ( [ 4 ]),,,, (e g [ 1 ]),, Gibbs,,,,,,
D esign and Imp lem en tation of Parallel Genetic A lgo rithm
2004 6 6 : 100026788 (2004) 0620061206 1, 2, 1 2, (1., 230027; 2., 230039) : PGA, : ; ; : T P301. 6 : A D esign and Imp lem en tation of Parallel Genetic A lgo rithm fo r F inding Roo ts Comp lex Functional
A Knowledge M odel for D esign of Population Nutr ien t Index D ynam ics in W heat
2005, 25 (3): 47 52 Journal of T riticeae C rop s Ξ,,,, ( g,, 210095) :,,,,, ( ) 2,, RM S E 8. 13 kgghm 2, RM S E 0. 20%, : ; ; ; ; : S 512. 1; S 311 : A : 100921041 (2005) 0320047206 A Knowledge M odel
YU Yong2ting, X IE Yuan2yuan, HUAN G L i2li, KAN G Zhen2sheng
35 2 ( ) V o l. 35 N o. 2 2007 2 Journal of N o rthw est A & F U niversity (N aṫ Sci. Ed. ) Feb. 2007 Β21, 32 α,,, (, 712100) [ ] M S,, 7, 14, 21 d (26, 150 rgm in), 3, 52 (DN S) Β21, 32,,, Β21, 32,,,,,
Im pact of capac ity a lloca tion on bullwh ip effect in supply cha in
7 4 00 8 JOU RNAL O F SYST EM S EN G IN EER IN G V o l 7 N o 4 A ug 00 ( 30007) : - - - : ; ; ; ; : F5 : A : 000578 (00) 04034009 Im pact of capac ity a lloca tio o bullwh ip effect i supply cha i W AN
60 s 72, 60 s 94, 30 s; 5
342 Ch inese Journal of Parasito logy and Parasitic D iseases 3 510515 :, CS : PCR CS, M 13, 3 M 132CSP DNA, PCGEN E CS : (PFD - 3gYN ) T 4 W ellcom e N F54 3D 7 7G8 CS, P. f T hgt c : CS (circum spo rozo
(TPP)Co 1. 1 ], [ 1, 2 ] V o l. 3 N o. 4 N ov EL ECTROCH EM ISTR Y ) ) ,, Co V V. (T PP) Co (Py) , - 1.
![(TPP)Co 1. 1 ], [ 1, 2 ] V o l. 3 N o. 4 N ov EL ECTROCH EM ISTR Y ) ) ,, Co V V. (T PP) Co (Py) , - 1.](/thumbs/93/113605453.jpg "(TPP)Co 1. 1 ], [ 1, 2 ] V o l. 3 N o. 4 N ov EL ECTROCH EM ISTR Y ) ) ,, Co V V. (T PP) Co (Py) , - 1.")
3 4 1997 11 EL ECTROCH EM ISTR Y V o l 3N o 4 N ov 1997 (TPP)Co a 3 b a a (a 110015) (b 230026) (Py) (T PP)Co 1 22 Py Co (g ) gco (g ) 0 25-0 40 V (vṡ SCE) Py Co (g ) gco (g ) 0 25 V - 0 40 V Py Co (g
CHEM ICAL JOU RNAL O F CH IN ESE UN IV ERS IT IES , 20% 30%. . ( A spart pv K100. :
V ol. 25 N o. 6 2 0 0 4 6 CHEM ICAL JOU RNAL O F CH IN ESE UN IV ERS IT IES 10601064 [B10Glu, B24-A sp-b25 ],,, (,, 100871) PCR, B B 10 H is Glu, B 24 B 25 A sp, [B 10Glu, B 242A sp2b 25 ]. pbv 220, DH
M eth, 1987; 16: 29. (P < 0. 05)
HCM V g PCR,, HCM V g, HCM V g,,, lococcus. A cta P aed iatr S cand, 1989; 78: 222. 3.,,,. PCR 1. V onso sover A. D etecton of CM V in urine: comparison betw een DNA - DNA hybridizaton, virus iso lation
A R, H ilbert2h uang T ran sfo rm and A R M odel
004 10 10 : 10006788 (004) 1000906 H ilberth uang A R,, (, 41008) : H ilberth uang A R H ilberth uang IM F ( Intrinsic M ode Function), IM F, IM F A R, M ahalanobis,, : H ilberth uang ; ; ; A R ; ; : TH
1. 1 R x (Strain R x of m arine Pho to syn thetic Bacteria )
32 9 () V o l. 32 N o. 9 2004 9 Jour. of N o rthw est Sci2T ech U niv. of A gri. and Fo r. (N aṫ Sci. Ed. ) Sep. 2004 Rx Ξ 1, 2, 2, 1, 2 (1, 110016; 2, 110161) [ ] R x, DNA G+ C, R x, (0. 5 0. 7) Λm (1.
1 g, , CO 2 5% CO 2 , 37,, Jour. of N o rthw est Sci2T ech U niv. of A gri. and Fo r. (N aṫ Sci. Ed. ) M arch 2004 , 2
32 3 ( ) V o l. 32 N o. 3 2004 3 Jour. of N o rthw est Sci2T ech U niv. of A gri. and Fo r. (N aṫ Sci. Ed. ) M arch 2004 Ξ,,,, (, 712100) [ ],,,, ;,, ;,, ( );,,,,, ;, 2 4 ;, ; ; ; [ ] ; ; ; [ ] S827. 9
Advances in tissue culture of A rnebia euch rom a
Κ2004Κ24; 8ΓΠ1560 1564 A cta B oṫ B orea l. -O cciden ṫ S in. Π100024025; 2004Γ0821560205 Ξ 1Κ2 13 1 2 1 ; 1 Κ 100093Μ2 Κ 100037Γ Π Κ Κ Κ Π Μ Μ ΠQ 943. 1 ΠA Advances in tissue culture of A rnebia euch
The Changes of Card iova scular Respon se to O rthosta tic Stress Caused by Hypovolem ia Inuced by W e ightlessness: A Sim ula tion Study
J B iom ed Eng 2002; 19 (1) 48 52 3 1 2 1 2 2 1 ( 100084) 2 ( 710032) M elch io r (1994) ( ) 15% ; 5% LBN P HR BP The Changes of Card iova scular Respon se to O rthosta tic Stress Caused by Hypovolem ia
, 4, 6, 8 m in; 30%, 50%, 70%, 90% 100% 15, 30, 45, 60, s V c
32 6 ( ) V o l. 32 N o. 6 2004 6 Jour. of N o rthw est Sci2T ech U niv. of A gri. and Fo r. (N aṫ Sci. Ed. ) June 2004 Vc Ξ 1, 2, 2, 1, 3, 3 (1, 712100; 2, 450002; 3, 453003) [ ], V c, V c, V c ; V c,