T IM P-1 cd NA CO S-7. Clon ing of Human T IM P-1 cd NA and its Expression in COS-7 Cells. 21 (tissue in2
|
|
|
- Ἀριστοτέλης Ιωάννου
- 5 χρόνια πριν
- Προβολές:
Transcript
1 ISSN CN gQ 16 3 Ch in. J. B iochem. M o l. B io l. 2000, V o l. 16, N o. 3, T IM P-1 cd NA 3 CO S (,,, ) GenBank T IM P21, R T 2PCR T IM P21 cdna T 2A pcr R 2. 1, DNA T IM P21 cdna pcdna 3, pcdna 3gT IM P21, DO TA P CO S2 7,N o rthern CO S27 T IM P21, T IM P21 CO S27, T IM P21 T IM P21,, CO S27 Q 78 Clon ing of Human T IM P-1 cd NA and its Expression in COS-7 Cells L IN Hong2li, CH EN X iang2m ei 3 3, YU Zh i2heng, L IW en2ge, FU Bo (D ep artm ent of N ep h rology, General H osp ital of Ch inese PL A,M ilitary K id ney Center, B eij ing , Ch ina) Abstract T IM P21 fu ll length cdna con tain ing the signal pep tide w as am p lified by reverse tran2 scrip tion and po lym erase chain reaction (PCR ) from to tal RNA of hum an no rm al renal tissueṡ Confirm ed by the DNA sequence analysis, the T IM P21 cdna fragm en ts w ere then in serted to m amm alian exp ression p lasm id pcdna 3 respectively. T hu s pcdna 3gT IM P21 recom b inan t eu2 karyo tic exp ression vecto r w as con structed. M o reover, th is recom b inan t p lasm id w as tran sfected in to CO S27 cells w ith lipofectin DO TA P. N o rthern b lo t analysis and in situ hyb ridization dem on2 strated that no T IM P21 m RNA w as detected in no rm al CO S27 cells, w h ile T IM P21 m RNA w as detected in pcdna 3gT IM P21 tran sfected CO S27 cells. Fu rther study indicated that the endoge2 nou s T IM P21 overexp ression cou ld induce the p ro liferation of CO S27 cells. Key words T IM P21, C lon ing, T ran sfection, CO S27 cells, : T IM P2 [ 1 1 ],, 21 (tissue in2, h ib ito r of m etallop ro teinase21, T IM P21) T IM P21,, T IM P21, (m atrix m etallop ro teinases,mm P s) MM P s 3 (962056) 3 3 T el: (010) , Fax: (010) E2m ail: xm chen@public. bta. neṫ cn T IM P21 T IM P21,, ,, : , :
2 3 : T IM P21 cdna CO S T IM P21 cdna, pcdna 3g T IM P21, 55, 30 s, 72, 2 m in, 72 (CO S27), N o rthern T aq DNA 15 m in 1% CO S27 T IM P21, T IM P2 1 T IM P TIM P-1 cd NA T-A CO S TRL ZO 1 T EL 2T EST Inc,DNA T 4 P rom ega 117 pcd NA3-TIM P-1 T 2A clon ing Invitrogen PCR L ipofectin T IM P21 A vag T IM P21 SP6gT 7 SuperScrip t TM RN ase H - G GibcoBRL DNA Q IN 2 CO S27 18 h 1 GEN 112 pcdna 3 DH 5Α CO S27, 400 ggl G418, 10 d 113 RNA 2 mm 2 mm 3 mm, TRL ZO 1,, 70%, D FPC 260 nm T GCA CGCA GG23 ; 3 : 5 2CCCGCTCA 2 RNA 114 GenBank T IM P21 1 m in, 25, 72 7 m in PCR T IM P21 cdna : T IM P21 cdna, 5 5 2T T GAA T TCCCA CCA T GGCCCC CT T T GA GCC23 ; 3 5 2CT GAA T TCGCA GGA T TCA GGCTA TCT G m in N, 1110 Northern TIM P-1 COS-7 E cor g 3 C, 15 g CO S27 RNA, [ 3 ] E cor g pcdna 3 neo r, neo r, 5 5 2CAA 2 GA T GGA T T GCA CGCA GG23 ; 5 2CC TIM P-1 COS-7 CGCTCA GAA GAA CTCGTC RT-PCR TIM P-1 cd NA SuperScrip t TM RN ase H - pcdna 3gT IM P21, T IM P21 (T 7) cdna R T V en t DNA (Sp 6)RNA CO S27 PCR PCR PCR (con) pcdna 3 (vec) pcdna 3g : DNA 94 3 m in, 10 T IM P21 (PT ) CO S27 3 cm 94, 15 s, 55, 30 s, 72, 1 m in, 15 94, 15 s, PCR Geneclean T 2A clon ing, T IM P21 PCR pcr R 2. 1, pcr R 2. 1 T 7 M 13 [ 3 ] E cor g 50 DO TA P pcdna 3 pcdna 3gT IM P21 CO S COS-7 neo r CO S27 DNA, PCR, 5 : 5 2CAA GA T GGA T GAA GAA CTCGTC23, 790 bp : 94, 30 s, 60, 30 s, 72, 652 bp : 94, 30 s, 58, 30 s, 72, 1 m in, 30, N o rthern, h D IG RNA L abelling K it (Sp 6gT 7),, 40, RNA 016 ggm l,
3 h, NBT gbc IP 1112 con vec PT CO S27 pcr R 2. 1, E cor g N cog gm l 24, pcr R 2. 1gT IM P21, F ig. 1, 6, 3, 5 d, 212 pcd NA3gTIM P-1 3 E cor g T IM P21 cdna pcr R RNA, R T 2PCR T IM P21 cdna, 652 bp T 2A T IM P21 cdna T IM P21, pcdna 3 ( F ig. 2 ), E cor g T IM P TIM P-1 cd NA cdna, 514 kp 652 bp ; F ig. 1 Partial sequence analysis of hum an T IM P21 cdna F ig. 2 Construction m ap of pcdna 3gT IM P21 recom binant p lasm id
4 3 : T IM P21 cdna CO S A vag T IM P21 cdna, bp bp 99 bp 3 (F ig. 3) 213 PCR neo r TIM P-1 COS-7 PCR pcdna 3 neo r, CO S27 pcdna 3gT IM P21 CO S bp CO S27 neo r, PCR CO S27 T IM P21cDNA, CO S27, pcdna 3gT IM P21 CO S bp, T IM P21cDNA CO S27, T IM P21 T IM P21 (F ig. 4) F ig. 3 Restriction m ap of recom binant p lasm id pcdna 3g T IM P21 L ane 1: 1 kb mo lecular w eigh t m arker; L ane 2: Recom binant p lasm id pcdna 3gT IM P21; L ane 3: pcdna 3gT IM P21 digested by E cor g ; L ane 4: pcdna 3gT IM P21 digested by A vag F ig. 4 Amp lification of neo r and T IM P21 cdna gene by PCR L ane 1: 1 kb mo lecular w eigh t m arker; L ane 2: CO S27 cells; L ane 3: CO S27 cells transfected by pcdna 3; L ane 4: CO S27 cells transfected by pcdna 3gT IM P COS T IM P21 m RNA CO S N o rthern b lo ting T IM P21 m RNA 7 : T IM P21 CO S27 : 15 g RNA (T 7) T IM P21 RNA (Sp 6) pcdna 3gT IM P21 CO S27 pcdna 3gT IM P21 RNA, 32 P CO S27, T IM P21, 1 h, CO S27 Sp 6 T 7, pcdna 3g CO S27 T IM P21 T IM P21 CO S27 (F ig. 5) m RNA ; CO S27
5 F ig. 5 N o rthern blo tting of hum an T IM P21 mrna in CO S27 cells 1: CO S27 cells; 2: CO S27 cells transfected by pcdna 3; 3: CO S27 cells transfected by pcdna 3gT IM P21 CO S27 ( ), pcdna 3gT IM P21 CO S27 pcdna 3gT IM P21 CO S27 T 7 T IM P21 (F ig. 6), Sp 6 F ig. 6 In situ hybridization of hum an T IM P21 mrna in CO S27 cells A, B: no rm al CO S27 cell; C, D: CO S27 cells transfected by pcdna 3gT IM P21. A, C: In situ hybridyzation w ith sense T IM P21 RNA p robe (T 7) ; B, D: In situ hybridyzation w ith antisense T IM P21 RNA p robe (Sp6) 215 TIM P-1 COS-7 24 h, F ig. 7 : T IM P21 (PT ) (con) (vec),
6 3 : T IM P21 cdna CO S (P < 0. 01), T IM P21 T IM P21, CO S27 F ig. 7 T he effects of T IM P21 transfection on the p ro lifera2 tion of CO S27 cells 3 3 P < (PT vs con) ; P < (PT vs vec) 3 T IM P21 cdna 782 bp, 63 bp 5, 623 bp 99 bp 3 B lood, 1998, 92: T IM P21 cdna, 652 bp V en t DNA R T 2 PCR m RNA, T aq DNA 3 25, PCR, T IM P21, Docherty [ 2 ] T IM P21, V en t PCR 3 A, V en t PCR T 2A, PCR T aq DNA 72 PCR 15 m in, 3 A,, pcr R 2. 1 T 7 M 13, [ 4 ], T IM P21, T IM P21 T IM P21 pcdna 3, CO S27 N o rthern, pcdna 3gT IM P21 CO S27 1 h T IM P21, : T IM P21 CO S27, T IM P21, T IM P21 H ayakaw a [ 5 ] T IM P21, T IM P21 pcdna 3gT IM P21 CO S27, T IM P21 CO S27, T IM P21 CO S27 (References) 1 Guedez L, Courtem anch L, Stetler2Stevenson M. T issue in2 hibito r of m etallop ro teinases21 ( T IM P21) induce differentia2 tion and an antiapop to tic pheno type in germ inal center B cellṡ 2 Docherty A J P, L yons A, Sm ith B J,W righ t E M, Stephens P E, H arris H J R. Sequence of hum an tissue inh ibito r of m etallo2 p ro teinases and its identity to erythiod2po tentiating activity. N atu re, 1985, 318: Sam brook J, F ritsch E F, M aniatis T. M olecu lar C loning : A L aboratory M anual, 2nd ed, N ew Yo rk: Co ld Sp ring H arbo r L abo rato ry P ress, Yo sh iji H, H arris S R, Raso E, Gom ez D E, L indsay C K, Sh ibuya M, Sinha C C, T ho rgeirsson U P. M amm ary cacinom a cells over2exp ressing tissue inhibito r of m etallop ro teinases21 show s enhanced vescular endo thelial grow th facto r exp ression. Int J Cancer, 1998, 75: H ayakaw a T, Yam ash ita K, T anzaw a K, U ch ijim a E, Iw ata K. Grow th2p romo ting activity of tissue inhibito r of m etallop ro2 teinases21 (T IM P21) fo r a w ide range of cells. F EB S, 1992, 298 (1) : 29 32
(A ntifreeze p roteins, A FP s) (afp ),
29 1 ( )V ol. 29 N o. 1 2001 2 Jouṙ of N orthw est Sci2Tech U niv. of A gri. and Foṙ (N aṫ Sci. Ed. ) Feb. 2001 α 1, 1, 2, 3 (1, 712100; 2, 710032; 3, 712100) []A utum n K ing, CTAB DNA, PCR (Polym erase
Ch in. J. B iochem. M o l. B io l. 2000, V o l. 16, N o. 5, Cd 2+ : Cd 2+ ΛCd 2+
ISSN 100727626 CN 1123870gQ 16 5 Ch in. J. B iochem. M o l. B io l. 2000, V o l. 16, N o. 5, 631 636 2000 10 ΑΑ-cD NA Cd 2+ 3 (,, 100871), Β, Α Α Β, ΑΑ PCR ΑΑ2cDNA A T G AA CA, CaM V 35S ΑΑ2cDNA pgptv
The Targeted Express ion of Human IL -2gIFNΑ2b Fused Gene in Hepa toma Cells
15 4 1999 8 V o l 15,N o 4 Ch inese Jou rnal of B iochem istry and M o lecu lar B io logy A ug, 1999 3 IL -2gIFNΑ2b (, 200032), DNA IL 22 IFN Α, A FP galb, EA FP2PALB A, IL 22gIFN Α2b A FP IL 22 IFN IL
acrysta ll iferous stra in Btk BE20
J ou rnal of N anj ing A g ricu ltu ral U niversity S18-4 - Btk BE20 3 1 2 2 2 2 ( 1, 321004; 2, 510275) S1824, PH T 3101, DNA P stl, B tk BE 20 SD S2PA GE, 2,, S1824 ; 2 ; ; S48217 Clon ing and expression
26 3 V o l. 26 N o A cta Eco logiae A n im alis Dom astici M ay ,
26 3 V o l. 26 N o. 3 2005 5 A cta Eco logiae A n im alis Dom astici M ay 2005,,,,, (, 524088) [ ] 1 200, 3,, 28 31, 83 87%, 1 2 0. 1% 0. 3% : 1 38 47 (P < 0. 05), 48 57 1 (P < 0. 05),,, 1 (P > 0. 05)
Supplemental file 3. All 306 mapped IDs collected by IPA program. Supplemental file 6. The functions and main focused genes in each network.
LIST OF SUPPLEMENTAL FILES Supplemental file 1. Primer sets used for qrt-pcr. Supplemental file 2. All 1305 differentially expressed genes. Supplemental file 3. All 306 mapped IDs collected by IPA program.
Expression and Pur if ica tion of W ild and M utan t Type NAD H-Cytochrom e b5 Reducta se in E. coli
ISSN 100727626 CN 1123870gQ 16 4 Ch in. J. B iochem. M o l. B io l. 2000, V o l. 16, N o. 4, 452 457 2000 8 NAD H- b5 (, 350025) NADH 2 b5, (b5r ), (C203Y) b5r cdna pgex22t, BL 21 W estern GST 2b5R 2Sepharo
Quan tif ica tion of Polym era se Cha in Reaction Products Using Solid Pha se Hybr id iza tion-enzym e Color im etr ic D etection
14 2 V o l114,n o12 1998 4 Ch inese Jou rnal of B iochem istry and M o lecu lar B io logy A p ṙ 1998 3 - (, 100044),,,, 125 g, :,,, Quan tif ica tion of Polym era se Cha in Reaction Products Using Solid
T. Kaw akam i. B ru ton. , Sf9
14 3 V o l114,n o13 1998 6 Ch inese Jou rnal of B iochem istry and M o lecu lar B io logy Jun 1998 Bruton T Kaw akam i (L a Jo lla Institute fo r A llergy and Imm uno logy, San D iego, CA, U SA ) ( ;,
Expression of Functiona l D oma in g of Ca lpa sta tin in E. coli and Prepara tion of An tiserum Aga in st Pur if ied GST-Ca lpa sta tin
ISSN 100727626 CN 1123870gQ 16 1 Ch in. J. B iochem. M o l. B io l. 2000, V o l. 16, N o. 1, 23 27 2000 2 g 3 1) 2) 2) 1) ( 1), 2), 100094) PCR (calpastatin) cdna g (404 bp ), pgex2kg pgex2calp 4 IPT G
cd NA ,, Expression and Character iza tion of Type-1 Pla sm inogen Activa tor Inh ibitor in P ich ia p astoris PA I21 , 1% 7 d, W estern b lo t
14 5 V o l114,n o15 1998 10 Ch inese Jou rnal of B iochem istry and M o lecu lar B io logy O cṫ 1998 g cd NA P p astoris 3 (, 200032) 379 PA I21 cdna AOX1 PHO 1, py IS21 P p astoris, H is + M u t -,, 1%
nested touch down PCR
33 2 () V o l. 33 N o. 2 2005 2 Jour. of N o rthw est Sci2T ech U niv. of A gri. and Fo r. (N aṫ Sci. Ed. ) Feb. 2005 nested touch down PCR CAPN1 EST Ξ 1, 2, 3, 2, 4, 3 (1, 712100; 2, 730070; 3, 100083;
( , ,
33 9 ( ) V o l. 33 N o. 9 2005 9 Jour. of N o rthw est Sci2T ech U niv. of A gri. and Fo r. (N aṫ Sci. Ed. ) Sep. 2005 Ξ 1, 2, 1, 1, 1, 1, 1, 2 (1 010018; 2 512005) [],, 8 8 9, () (),,,,,, ;, ;,, ;,,,
Ch inese Jou rnal of B iochem istry and M o lecu lar B io logy. (o steoarth ritis, OA )
ISSN 100727626 CN 1123870gQ 2001 4 Ch inese Jou rnal of B iochem istry and M o lecu lar B io logy 17 (2): 244 249 1) 1), 3, 2), 1), 1), 1), 2) 2), ( 1), 710032; 2), 100044) ( rheum ato id arth ritis, RA
China Academic Journal Electronic Publishing House. All rights reserved. O ct., 2005
23 10( 142) V ol. 23, N o. 10 200510 System s Engineering O ct., 2005 100124098 (2005) 1020081205 Ξ 1, 2, 1 2, (1., 710049; 21, 266033),,,,,,,, ; ; F224. 32 A 20 6070 [1, 2 ], 1, 1. 1, (1) T = {1, 2},
6 cm, 1. 2 IAA, NAA, 2. 1
30 6 ( ) V o l. 30 N o. 6 2002 12 Jour. of N o rthw est Sci2T ech U niv. of A gri. and Fo r. (N aṫ Sci. Ed. ) D ec. 2002 Ξ 1, 2, 2, 3, 1, 4, 1 (1 ; 2 ; 3, 712100; 4, 733200) [],,,,,, ; 1g2 M S, 1g3M S
Ch in. J. B iochem. M o l. B io l. 2000, V o l. 16, N o. 6,
ISSN 100727626 CN 1123870gQ 16 6 Ch in. J. B iochem. M o l. B io l. 2000, V o l. 16, N o. 6, 814 819 2000 12 3 RNA 3 3 (, 200032) upa R RNA pu RA S M DA 2M B 2231, G418 N o rthern upa R RNA, R T 2PCR upa
GPS, 0. 5 kg ( In tegrated Fertility Index, IF I) 1. 1 SPSS 10. IF I =
34 11 () V o l. 34 N o. 11 2006 11 Jour. of N o rthw est Sci2T ech U niv. of A gri. and Fo r. (N aṫ Sci. Ed. ) N ov. 2006 α 1, 1, 2, 1 (1, 450002; 2, 410007) [ ]12 () 1 612,, ( IF I ) : (1), ( ) ( ), ph
A (GPA ), (Perco ll) A, Gene Express ion of Erythrocyte M em brane Glycophor in A in N IDDM
14 5 V o l114,n o15 1998 10 Ch inese Jou rnal of B iochem istry and M o lecu lar B io logy O cṫ 1998 g GPA 3 (, 410078) g (N IDDM ) A (GPA ), (Perco ll) 15 25 N IDDM, RNA, N o rthern GPA GP W estern, N
Con struction and B ioactiv ity of Cys57 M utan t of Human In testina l Trefo il Factor
ISSN 100727626 CN 1123870gQ 16 5 Ch in. J. B iochem. M o l. B io l. 2000, V o l. 16, N o. 5, 606 611 2000 10 57 3 (,, 100871) PCR C 57 h IT F pgex24t 21, IT PG, Glu tath ione2sepharo se 4B, Sephacryl S
(T rip tery g ium w ilf ord ii Hook) ,Beroza [6 ] 4 W ilfo rine W ilfo rdine W ilfo rgine W il2. Euon ine 1. 0% 1980, 1. 1 ; 1. 0%, ; 0.
![(T rip tery g ium w ilf ord ii Hook) ,Beroza [6 ] 4 W ilfo rine W ilfo rdine W ilfo rgine W il2. Euon ine 1. 0% 1980, 1. 1 ; 1. 0%, ; 0.](/thumbs/92/108790990.jpg "(T rip tery g ium w ilf ord ii Hook) ,Beroza [6 ] 4 W ilfo rine W ilfo rdine W ilfo rgine W il2. Euon ine 1. 0% 1980, 1. 1 ; 1. 0%, ; 0.")
34 12 ( ) V o l. 34 N o. 12 2006 12 Jour. of N o rthw est Sci2T ech U niv. of A gri. and Fo r. (N aṫ Sci. Ed. ) D ec. 2006 Ξ 1, 3, 1, 2, 1, 2, 1, 1, 2 (1, 712100; 2, 712100; 3, 450008) [ ], 0. 1%, 3 48
Effects of PD on IL -6- induced Growth Arrest and Term ina l D ifferen tia tion of M 1 Cells
ISSN 100727626 CN 1123870gQ 2001 2 Ch inese Jou rnal of B iochem istry and M o lecu lar B io logy 17 (1): 115 120 PD 098059 IL -6 M 1, 3,,, (, 100850) IL 26 M 1 R asgm A PK, M EK PD 098059 R asgm A PK,
Supplementary Table 1. Primers used for RT-qPCR analysis of striatal and nigral tissue.
Supplementary Table 1. Primers used for RT-qPCR analysis of striatal and nigral tissue. Gene Forward Primer (5-3 ) Reverse Primer (5-3 ) Dopaminergic Markers TH CTG GCC ATT GAT GTA CTG GA ACA CAC ATG GGA
D NA , ) SCGE DNA.
14 5 V o l114,n o15 1998 10 Ch inese Jou rnal of B iochem istry and M o lecu lar B io logy O cṫ 1998 SCGE D NA (, (, 030006) 030006) (SCGE) ; SCGE Χ DNA, Χ DNA, :,DNA,, Pr inc iple and M ethod of the Single
M olecular Clon ing of D eletion Br idge Fragm en t of Y unnanese
15 1 1999 2 V o l 15,N o 1 Ch inese Jou rnal of B iochem istry and M o lecu lar B io logy Feb 1999 Y unnanese ( A Χ Β) 0-3 (,,, 100005) Yunnanese ( A Χ Β) 0 2, 3, EM BL 3, 11 G Χ 617 kb 1115 kb 3 ( ) 1115
IRBB 4 (Xa24) IRBB 7 (Xa27) IRBB 21 (Xa221) 6
J ou rna l of N anj in ricu ltu ra l U n iversity Ξ F 1 (, 210095) 12 7 10 : Xa24 xa25 Xa27 xa213 Xa221 5 3 I 4 (Xa24) I 7 (Xa27) I 21 (Xa221) 6 5, F 1 KS266 ZJ2173, Xa27 Xa221 F 1, Xa24 F 1 Xa27 Xa221
) V o l. 40 N o. 6 : A : Q ,, W ard [ 1 ] (A v icenn ia m a rina) (K and elia cand el)
![) V o l. 40 N o. 6 : A : Q ,, W ard [ 1 ] (A v icenn ia m a rina) (K and elia cand el)](/thumbs/93/114276195.jpg ") V o l. 40 N o. 6 : A : Q ,, W ard [ 1 ] (A v icenn ia m a rina) (K and elia cand el)")
40 6 ( ) V o l 40 N o 6 2001 11 Jou rnal of X iam en U n iversity (N atu ral Science) N ov 2001 : 043820479 (2001) 0621350205,,,, (, 361005) :, (N R ), N R N R, N ac l ; N R : ( 0 30 ; 0 20 )N R, 30, 89
Human In sulin- like Growth Factorg in M ethylotroph ic Y ea st P. p astoris
15 6 1999 12 V o l. 15,N o. 6 Ch inese Jou rnal of B iochem istry and M o lecu lar B io logy D ec., 1999 g P. p astoris 3 (, 100005), : 5 IGF2g ; ; Α P. p astoris, IGF2g ;, 60 m ggl P. p astoris rh IGF2g,,N
Το άτομο του Υδρογόνου
Το άτομο του Υδρογόνου Δυναμικό Coulomb Εξίσωση Schrödinger h e (, r, ) (, r, ) E (, r, ) m ψ θφ r ψ θφ = ψ θφ Συνθήκες ψ(, r θφ, ) = πεπερασμένη ψ( r ) = 0 ψ(, r θφ, ) =ψ(, r θφ+, ) π Επιτρεπτές ενέργειες
(classical sw ine fever, CSF) (CSFV ) (O IE) A 15, 1999202212 96,, C2,, 20 70 80, 1. 1 SXZZ), 1. 2
31 5 () V o l. 31 N o. 5 2003 10 Jour. of N o rthw est Sci2T ech U niv. of A gri. and Fo r. (N aṫ Sci. Ed. ) O cṫ 2003 E2 gp55 Ξ 1, 1, 1, 1, 1, 2, 2 (1, 712100; 2, 730046) [ ] R T 2PCR PCR E 2,, A lfo
copula, 5 3 Copula Κ L = lim System s Engineering M ay., 2006 : (2006) ,,, copula Ξ A rch im edean copula (Joe,
24 5 ( 149 ) V ol. 24, N o. 5 2006 5 System s Engineering M ay., 2006 : 100124098 (2006) 0520088205 copula Ξ, (, 230052) : (copula), A rch im edean copula, Gum bel2hougard copula,, : Copula; ; : F830 :
: H IV. Screen ing of 1F7 Id iotyp ic An ti-gp160 An tibod ies from Phage An tibody L ibrary
14 1 V o l114,n o11 1998 2 Ch inese Jou rnal of B iochem istry and M o lecu lar B io logy Feb 1998 H IV-1 gp 160 2) 1) 1) 1) 3) (, 100850; 2), 100071; 3), 130062) : H IV m RNA, IgG Fd Fab, pcom b3, XL
TR IBOLO GY N ov, 2004 , P I2. , 120 M Pa, P I. 6 mm 7 mm 30 mm [7, 8 ] mm,. JSM 25600LV 1. 1 (EDXA ).
![TR IBOLO GY N ov, 2004 , P I2. , 120 M Pa, P I. 6 mm 7 mm 30 mm [7, 8 ] mm,. JSM 25600LV 1. 1 (EDXA ).](/thumbs/50/26571605.jpg "TR IBOLO GY N ov, 2004 , P I2. , 120 M Pa, P I. 6 mm 7 mm 30 mm [7, 8 ] mm,. JSM 25600LV 1. 1 (EDXA ).")
24 6 V o l 24, N o 6 2004 11 TR IBOLO GY N ov, 2004,,, (, 730000) : MM 2200 (PT FE) M os2 (P I) GC r15, X P I, PT FE M os2 P I, P I+ 30%M os2, P I 50%. P I+ 10% PT FE+ 20%M os2, P I, P I+ 20% PT FE+ 10%M
Postna ta l D evelopm en ta l Expression of Phosphoprote in s in Synaptosom e
14 1 V o l114,n o11 1998 2 Ch inese Jou rnal of B iochem istry and M o lecu lar B io logy Feb 1998 3 (, 100875),,, (PND ) 3 d 7 d 21 d,, ; PND 21d, :,,, Postna ta l D evelopm en ta l Expression of Phosphoprote
T he Op tim al L PM Po rtfo lio M odel of H arlow s and Its So lving M ethod
2003 6 6 00026788 (2003) 0620042206 H arlow, 2 3, (., 70049; 2., 7006; 3., 200433) H arlow,,,,, ;, ; ; F832. 5; F830. 9 A T he Op tim al L PM Po rtfo lio M odel of H arlow s ad Its So lvig M ethod W AN
Investiga tion on L ipoprote in (a) Receptor in the M em brane of M onkey L iver Cells
ISSN 100727626 CN 1123870gQ 16 1 Ch in J B iochem M o l B io l 2000, V o l 16, N o 1, 96 100 2000 2 (a) 3 (, 310009; 3, 030001) SD S2PA GE, LDL LDL L p (a) PL G 3, 370 kd 290 kd 80 kd, L p (a) + PL G L
, 4, 6, 8 m in; 30%, 50%, 70%, 90% 100% 15, 30, 45, 60, s V c
32 6 ( ) V o l. 32 N o. 6 2004 6 Jour. of N o rthw est Sci2T ech U niv. of A gri. and Fo r. (N aṫ Sci. Ed. ) June 2004 Vc Ξ 1, 2, 2, 1, 3, 3 (1, 712100; 2, 450002; 3, 453003) [ ], V c, V c, V c ; V c,
Ch in. J. B iochem. M o l. B io l. 2000, V o l. 16, N o. 4, cd NA
ISSN 100727626 CN 1123870gQ 16 4 Ch in. J. B iochem. M o l. B io l. 2000, V o l. 16, N o. 4, 524 528 2000 8 cd NA 3 (, 100036) (T PO ) T PO pcdna 3hT PO pcdna 3, EL ISA T PO ( IGF ) 2g g (PD GF), T PO,
A R, H ilbert2h uang T ran sfo rm and A R M odel
004 10 10 : 10006788 (004) 1000906 H ilberth uang A R,, (, 41008) : H ilberth uang A R H ilberth uang IM F ( Intrinsic M ode Function), IM F, IM F A R, M ahalanobis,, : H ilberth uang ; ; ; A R ; ; : TH
Η νέα προσέγγιση στην ταχεία προγεννητική διάγνωση των χρωµοσωµατικών ανωµαλιών του εµβρύου
Αµνιο-PCR Η νέα προσέγγιση στην ταχεία προγεννητική διάγνωση των χρωµοσωµατικών ανωµαλιών του εµβρύου Αγγελική Χατζάκη, PhD Γεωργία Χριστοπούλου, MSc Τµήµα Γενετικής και Μοριακής Βιολογίας Μαιευτήριο «ΜΗΤΕΡΑ»
V irus ide ntifica tion a nd sc re e ning v irus ic ide s of pe ppe r v irus d ise a se in S ha a nxi P rov ince
35 1 () V o l. 35 N o. 1 2007 1 Journal of N o rthw est A & F U niversity (N aṫ Sci. Ed. ) Jan. 2007 Ξ 1, 2, 2, 1, 1 (1, 712100; 2, 710003) [] 126, 5, DA S2EL ISA,, (CM V ) (TM V ) (T EV ) Y (PV Y) (BBWV
Ch in. J. B iochem. M o l. B io l. 2000, V o l. 16, N o. 3, U CP. (P < 0101) ; U CP 2 m RNA , R T 2PCR
ISSN 100727626 CN 1123870gQ 16 3 Ch in. J. B iochem. M o l. B io l. 2000, V o l. 16, N o. 3, 394 399 2000 6 3-1 2 3 3 3 (,, 100730) 21 2 3 (U CP1, 2, 3), R T 2PCR U CP 1, 2, 3 m RNA U CP 1. U CP 2, 3,
Jour. of N o rthw est Sci2T ech U niv. of A gri. and Fo r. (N aṫ Sci. Ed. ) Sep , 1 [7 ] ,, 2 ,, 1 85% [1 4 ], [ ]
![Jour. of N o rthw est Sci2T ech U niv. of A gri. and Fo r. (N aṫ Sci. Ed. ) Sep , 1 [7 ] ,, 2 ,, 1 85% [1 4 ], [ ]](/thumbs/49/25047659.jpg "Jour. of N o rthw est Sci2T ech U niv. of A gri. and Fo r. (N aṫ Sci. Ed. ) Sep , 1 [7 ] ,, 2 ,, 1 85% [1 4 ], [ ]")
33 9 () V o l. 33 N o. 9 2005 9 Jour. of N o rthw est Sci2T ech U niv. of A gri. and Fo r. (N aṫ Sci. Ed. ) Sep. 2005 60 Co Χ Ξ,,, (, 712100) [ ] 3 60 Co Χ, 3,, 60 Co Χ, Χ, ; 60 Co Χ,,,, ;, 60 Co Χ, 3,
IL -4 . IL 24, cp IL 42PE38KD EL,.
V o l. 26 N o. 5 2 0 0 5 5 CH EM ICAL JOU RNAL O F CH IN ESE UN IV ERS IT IES 874 879 IL -4 1, 2, 3, 1, 2, 1, 2 (1., 110016; 2., 100039; 3. Beckm an R esearch Institute, C ity of Hope N ationalm edical
(P seudo rab ies, PR ) (A u jeszky, s D isease, AD ), (Pesuderab ies viru s, PRV )
34 9 ( ) V o l. 34 N o. 9 2006 9 Jour. of N o rthw est Sci2T ech U niv. of A gri. and Fo r. (N aṫ Sci. Ed. ) Sep. 2006 W G Ξ 1, 1, 1, 1, 1, 2 (1, 712100; 2, 723600) [ ]ge2el ISA 6 187 (P seu2 do rabies
(M u ltidrug resistance, M DR )
V o l. 26 N o. 8 2 0 0 5 8 CH EM ICAL JOU RNAL O F CH IN ESE UN IV ERS IT IES 1446 1450 1, 1, 2 (1., 132013; 2., 130023) V CR Bel27402 M DR,.,, V CR. Bel27402, V CR, V CR G2, P2170, M DR 1gmRNA, Topo gαmrna.
The Clon ing of PS-2 Gene and Its Expression in Hepa tocellular Carc inoma
ISSN 100727626 CN 1123870gQ 16 1 Ch in. J. B iochem. M o l. B io l. 2000, V o l. 16, N o. 1, 1 5 2000 2 PS-2 1) 2) 3 (, 1), 510089; 2), 100083) m RNA, 14 N o rthern b lo t 9 500 bp cdna,,, PS22 (p resen
OPN antisense oligodeoxynucleotide inhibits p roliferation and apop tosis of human breast carcinoma cell line
2009 8 29 8 Basic & C linical M edicine August 2009 Vol. 29 No. 8 : 1 00 1 263 25 ( 2 0 0 9 ) 0 8 20 8 5 0 20 5 13, 2, 3, 4, 5 (11, 730030; 21, 730000; 31, 730000; 41, 730000; 51, 730020) : (OPN ) mrna
D esign and Imp lem en tation of Parallel Genetic A lgo rithm
2004 6 6 : 100026788 (2004) 0620061206 1, 2, 1 2, (1., 230027; 2., 230039) : PGA, : ; ; : T P301. 6 : A D esign and Imp lem en tation of Parallel Genetic A lgo rithm fo r F inding Roo ts Comp lex Functional
V o l122, N o13 M ay, 2003 PRO GR ESS IN GEO GRA PH Y : (2003) , : TU 984. , (Eco logy fo r evil) [1 ] (R ich Boyer), 2.
![V o l122, N o13 M ay, 2003 PRO GR ESS IN GEO GRA PH Y : (2003) , : TU 984. , (Eco logy fo r evil) [1 ] (R ich Boyer), 2.](/thumbs/93/114354980.jpg "V o l122, N o13 M ay, 2003 PRO GR ESS IN GEO GRA PH Y : (2003) , : TU 984. , (Eco logy fo r evil) [1 ] (R ich Boyer), 2.")
22 3 2003 5 PRO GR ESS IN GEO GRA PH Y V o l122, N o13 M ay, 2003 : 100726301 (2003) 03203162010, (, 710061) :, (R ich Boyer), 2001,, : ; ; ; ; : TU 984 1,,, (Eco logy fo r evil) [1 ], ( ), (D avid1 Sm
F ig. 1 Flow chart of comprehen sive design in the research of com patibil ity. E2m ail: ac. cn
V o l. 25 N o. 9 2 0 0 4 9 CH EM ICAL JOU RNAL O F CH IN ESE UN IV ERS IT IES 1632 1636,,,, (, 116011) H PL C2PAD 2M S,,.,. H PL C2PAD 2M S.,.,,. 2 ; ; O 652. 7 A 025120790 (2004) 0921632205,,., [1 5 ]
IL - 13 /IL - 18 ELISA PCR RT - PCR. IL - 13 IL - 18 mrna. 13 IL - 18 mrna IL - 13 /IL Th1 /Th2
344 IL - 13 /IL - 18 1 2 1 2 1 2 1 2 1 2 3 1 2 13 18 IL - 13 /IL - 18 10% / OVA /AL OH 3 5% 16 ~ 43 d 44 d ELISA BALF IL - 13 IL - 18 PCR RT - PCR IL - 13 IL - 18 mrna IL - 13 mrna 0. 01 IL - 18 mrna 0.
Appendix B Table of Radionuclides Γ Container 1 Posting Level cm per (mci) mci
3 H 12.35 Y β Low 80 1 - - Betas: 19 (100%) 11 C 20.38 M β+, EC Low 400 1 5.97 13.7 13 N 9.97 M β+ Low 1 5.97 13.7 Positrons: 960 (99.7%) Gaas: 511 (199.5%) Positrons: 1,199 (99.8%) Gaas: 511 (199.6%)
PUR IF ICATION AND CHARACTER IZATION OF EXTRACELL ULAR PROTEASE ECPASE 54 FROM A EROM ONAS H YD RO PH ILA
1 9 9 6, 1 9 ( 3 ) : 8 8 9 4 J ou rna l of N anj ing A g ricu ltu ra l U n iversity ECPase 54 Ξ 3 3 (, 210095) (A h) J21 56 (ED TA ) (PM SF), (ECPase), A h J 21 SD S2PA GE, 37,, 5 A h J21 D EA E2 Sephadex
The Changes of Card iova scular Respon se to O rthosta tic Stress Caused by Hypovolem ia Inuced by W e ightlessness: A Sim ula tion Study
J B iom ed Eng 2002; 19 (1) 48 52 3 1 2 1 2 2 1 ( 100084) 2 ( 710032) M elch io r (1994) ( ) 15% ; 5% LBN P HR BP The Changes of Card iova scular Respon se to O rthosta tic Stress Caused by Hypovolem ia
ΜΟΡΙΑΚΕΣ ΜΕΘΟΔΟΙ ΚΡΙΤΗΡΙΑ ΕΠΙΛΟΓΗΣ ΑΞΙΟΛΟΓΗΣΗ
- - ό ό : Ω ί ό ί ώ.. ά ή ί ός ή ς ύ ί ί ή έ ώ ό. ή ς ά ς ής ό ός ά ς ή έ ός ή ς ί ής ί ς. ό έ ώ - ώ ή ής ή ς- ί ά ά ς ί ς. ά ί ί έ έ ά ύ ή, ό ί ά, ό ό ά έ ά ής ί ύ George Wald ή ί έ ς ί ύ ό ς ί ς ά έ
ΓΗ ΚΑΙ ΣΥΜΠΑΝ. Εικόνα 1. Φωτογραφία του γαλαξία μας (από αρχείο της NASA)
ΓΗ ΚΑΙ ΣΥΜΠΑΝ Φύση του σύμπαντος Η γη είναι μία μονάδα μέσα στο ηλιακό μας σύστημα, το οποίο αποτελείται από τον ήλιο, τους πλανήτες μαζί με τους δορυφόρους τους, τους κομήτες, τα αστεροειδή και τους μετεωρίτες.
60 s 72, 60 s 94, 30 s; 5
342 Ch inese Journal of Parasito logy and Parasitic D iseases 3 510515 :, CS : PCR CS, M 13, 3 M 132CSP DNA, PCGEN E CS : (PFD - 3gYN ) T 4 W ellcom e N F54 3D 7 7G8 CS, P. f T hgt c : CS (circum spo rozo
1999, 17 (1): J ourna l of W uhan B otan ica l Resea rch ( ) ( ) 2, 3. (Celosia cristata L. ),
1999, 17 (1): 15 20 J ourna l of W uhan B otan ica l Resea rch Ξ ( 210013) (210095),, W HO g FAO, 3, 10, 3 ( ) 2317% 2714%,, 83147% 86194%, (EAA ) 4012% 4117%, (M et+ Cys) 10,,,,,,,,,,, 1 (Celosia cristata
DY NAM IC CHANGE OF EP ID ERM AL STEM CELL S IN THE WOUND HEAL ING COURSE OF D IABETIC RATSg
693 (diabetes m ellitus, DM ) (ep iderm al stem cells, ESC s), ESC s DM 48 W istar,, 180 200 g DM, 24 DM DM 115 cm 118 cm 2154 cm 2 ( 96 ), 3 7 14 21, ;, H E 19 (keratin 19, K19) Β1, 3 7 14 21, 24148%
Estimation of grain boundary segregation enthalpy and its role in stable nanocrystalline alloy design
Supplemental Material for Estimation of grain boundary segregation enthalpy and its role in stable nanocrystalline alloy design By H. A. Murdoch and C.A. Schuh Miedema model RKM model ΔH mix ΔH seg ΔH
Use of Site-D irected M utagenesis to Investiga te the Properties of Recom binan t H irud in-hv2
14 1 V o l114,n o11 1998 2 Ch inese Jou rnal of B iochem istry and M o lecu lar B io logy Feb 1998 (, 100101),,, rhv 2 47 11 rhv 2 Gln11 A sn47 H is11 L ys47, rhv 22H 11 30%, rhv 22K47 61% K i, rhv 22
N-acetyl- l-cyste ine im proves function of islet Β cell in hyperl ip idem ic rats and its m echan ism. V o l 36 N o , NA C PCR
36 6 2007 ( ) JOU RNAL O F ZH EJ IAN G UN IV ERS IT Y (M ED ICAL SC IEN CES) V o l 36 N o 6 2007 h ttp: www. journals. zju. edu. cngm ed Β 1, 1, 1, 1, 1, 1, 2 ( 1. 2., 100029) [ ] : 2N 2 (N 2acetyl2l2cysteine,
UPTAKE OF CAROTENO ID S BY INTESTINAL M UCOUS CELL S OF LAY ERS AND GOATS IN V ITRO
1997, 20 (4): 54 59 J ou rna l of N anj ing A g ricu ltu ra l U n iversity Ξ (, 210095) (Β2 ) : Β2, Β2 (P > 0. 05);, ( 15 Λmo lgl ) Β2 ; Tw een 20, ; (Υ) 013% Tw een 20 Β2 A ; 015 mo lgl Β2 (P < 0. 01)
Νόµοςπεριοδικότητας του Moseley:Η χηµική συµπεριφορά (οι ιδιότητες) των στοιχείων είναι περιοδική συνάρτηση του ατοµικού τους αριθµού.
Νόµοςπεριοδικότητας του Moseley:Η χηµική συµπεριφορά (οι ιδιότητες) των στοιχείων είναι περιοδική συνάρτηση του ατοµικού τους αριθµού. Περιοδικός πίνακας: α. Είναι µια ταξινόµηση των στοιχείων κατά αύξοντα
CHEM ICAL JOU RNAL O F CH IN ESE UN IV ERS IT IES , ( ECV 304) (angiogenesis),, :
V ol. 24 o. 2 2 0 0 3 2 CHEM ICAL JOU RAL O F CH I ESE U IV ERS IT IES 260 264 1, 3- -1, 3- -2H- 1, 2, 2, 2 (1. ; 2., 610041) ΑvΒ3 R GD,, 5 52 21, 32 21, 32 22H 2,, ( ECV 304) R GD ; ; 1, 32 21, 32 22H
1 g, , CO 2 5% CO 2 , 37,, Jour. of N o rthw est Sci2T ech U niv. of A gri. and Fo r. (N aṫ Sci. Ed. ) M arch 2004 , 2
32 3 ( ) V o l. 32 N o. 3 2004 3 Jour. of N o rthw est Sci2T ech U niv. of A gri. and Fo r. (N aṫ Sci. Ed. ) M arch 2004 Ξ,,,, (, 712100) [ ],,,, ;,, ;,, ( );,,,,, ;, 2 4 ;, ; ; ; [ ] ; ; ; [ ] S827. 9
LfcinB. Expression and Iden tif ica tion of Lfc inb Gene in P ich ia pastoris
265 ( LfcinB) 3 ( 518057) : ( Pichia pastors) ( bovine lactoferricin, Lf2 cinb), LfcinB pp IC9K, pp IC9K2LfcinB Sal, SMD1168 G418,, PCR LfcinB LfcinB, : (LfcinB),,, : Q782, Q784, Q786 : A : 025322654 (2007)
,, 1 mm,, : 250 g, 200 g, 120 g, 50 g 1. 1. 2 50 2 450 g T, 80, , ;,, ,,,,, 1. 2. 1%, 2%, 3% 2005209230 11230, 15 d, 60 d ( ) 1 2 1.
34 6 ( ) V o l. 34 N o. 6 2006 6 Jour. of N o rthw est Sci2T ech U niv. of A ri. and Fo r. (N aṫ Sci. Ed. ) Jun. 2006 Ξ,, (, 712100) [ ] 80 50 T,, 16,,, 1%, 2%, 3%,,,,,, 2% T,LH, FSH (P < 0. 05); T, L
J. Dairy Sci. 93: doi: /jds American Dairy Science Association, 2010.
Supplementary Table 1. Primers and PCR conditions used for the amplification of the goat SCD1 cdna (PCR1 to PCR6) and three SCD1 polymorphic regions (PCR7 to PCR9) PCR Primers Sequence Position 1 Thermal
U (x, y ) = : K (x i- x k) K (x i- x k, y j- y l), 2. 1
22 5 V o l 22, N o 5 2002 9 TR IBOLO GY Sep, 2002,, (, 100084) :, (FFT )., 2 (Green ),,, 3,. ; ; FFT; ; TH 117. 2 A 100420595 (2002) 0520390205,.,,,. L ub rech t [1 ]. (FFT ) [2 5 ]., [4, 5 ].,L iu [6
ΚΕΦΑΛΑΙΟ Α 1. ΕΙΣΑΓΩΓΗ 2. ΙΑΚΡΙΣΗ ΠΕΤΡΩΜΑΤΩΝ
ΚΕΦΑΛΑΙΟ Α ΕΡΕΥΝΑ ΠΕ ΙΟΥ 1. ΕΙΣΑΓΩΓΗ Όλα τα τεχνικά έργα κατασκευάζονται στην επιφάνεια του εδάφους ή κάτω απ αυτήν. Η ασφαλής τους θεµελίωση προϋποθέτει καλή γνώση των φυσικών και µηχανικών ιδιοτήτων
ΠΕΡΙΟΔΙΚΟΣ ΠΙΝΑΚΑΣ ΣΤΟΙΧΕΙΩΝ
ΠΕΡΙΟΔΙΚΟΣ ΠΙΝΑΚΑΣ ΣΤΟΙΧΕΙΩΝ Περίοδοι περιοδικού πίνακα Ο περιοδικός πίνακας αποτελείται από 7 περιόδους. Ο αριθμός των στοιχείων που περιλαμβάνει κάθε περίοδος δεν είναι σταθερός, δηλ. η περιοδικότητα
ΠΤΥΧΙΑΚΗ ΕΡΓΑΣΙΑ ΕΦΑΡΜΟΓΗ ΤΟΥ ΕΡΓΑΛΕΙΟΥ BALANCED SCORECARD ΣΕ ΙΔΙΩΤΙΚΟ ΝΟΣΟΚΟΜΕΙΟ. Σπουδαστές: Δεληλίγκα Αργυρούλα, ΑΜ: 2008057
ΤΕΧΝΟΛΟΓΙΚΟ ΕΚΠΑΙΔΕΥΤΙΚΟ ΙΔΡΥΜΑ ΚΑΛΑΜΑΤΑΣ ΣΧΟΛΗ ΔΙΟΙΚΗΣΗΣ ΟΙΚΟΝΟΜΙΑΣ ΤΜΗΜΑ ΜΟΝΑΔΩΝ ΥΓΕΙΑΣ ΚΑΙ ΠΡΟΝΟΙΑΣ ΠΤΥΧΙΑΚΗ ΕΡΓΑΣΙΑ ΕΦΑΡΜΟΓΗ ΤΟΥ ΕΡΓΑΛΕΙΟΥ BALANCED SCORECARD ΣΕ ΙΔΙΩΤΙΚΟ ΝΟΣΟΚΟΜΕΙΟ Σπουδαστές: Δεληλίγκα
Ch in. J. B iochem. M o l. B io l. 2000, V o l. 16, N o. 2, K 3 (A 6D )
ISSN 100727626 CN 1123870gQ 16 2 Ch in. J. B iochem. M o l. B io l. 2000, V o l. 16, N o. 2, 234239 2000 4 (P I4-K) 3 1) (,, 100101; 1), 100871) (P I 42K) (A 6D ) 2, 71510 6 610 8 (m o lgl ) - 1 11910-7
A ( SPA ) Su lfo succin im idyl 62[ 3 2( 22pyridyldith io ) p rop ionam ido ]hexanoate (Su lfo2l C2SPD P) C E2m ail: w
![A ( SPA ) Su lfo succin im idyl 62[ 3 2( 22pyridyldith io ) p rop ionam ido ]hexanoate (Su lfo2l C2SPD P) C E2m ail: w](/thumbs/91/105112871.jpg "A ( SPA ) Su lfo succin im idyl 62[ 3 2( 22pyridyldith io ) p rop ionam ido ]hexanoate (Su lfo2l C2SPD P) C E2m ail: w")
V o l. 25 N o. 1 2 0 0 4 1 CH EM ICAL JOU RNAL O F CH IN ESE UN IV ERS IT IES 44 49 -C 1, 1, 1, 1, 2, 3 (1., 400038; 2., 400032; 3. 26, 400060) A T 10M H z, C, 2 5 2C.. 215 16010 m IU gl C 01375 1210 nggml,
5 Ι ^ο 3 X X X. go > 'α. ο. o f Ο > = S 3. > 3 w»a. *= < ^> ^ o,2 l g f ^ 2-3 ο. χ χ. > ω. m > ο ο ο - * * ^r 2 =>^ 3^ =5 b Ο? UJ. > ο ο.
728!. -θ-cr " -;. '. UW -,2 =*- Os Os rsi Tf co co Os r4 Ι. C Ι m. Ι? U Ι. Ι os ν ) ϋ. Q- o,2 l g f 2-2 CT= ν**? 1? «δ - * * 5 Ι -ΐ j s a* " 'g cn" w *" " 1 cog 'S=o " 1= 2 5 ν s/ O / 0Q Ε!θ Ρ h o."o.
Stem Character istics of W heat w ith Stem L odging and Effects of L odging on Gra in Y ield and Qual ity
2006, 26 (1): 87 92 Journal of T riticeae C rop s Ξ,,,,, ( g, 225009) : 6,,,,,,, : ; ; ; ; : S 512. 1; S 318 : A : 100921041 (2006) 0120087206 Stem Character istics of W heat w ith Stem L odging and Effects
Cellular Physiology and Biochemistry
Original Paper 2016 The Author(s). 2016 Published The Author(s) by S. Karger AG, Basel Published online: November 25, 2016 www.karger.com/cpb Published by S. Karger AG, Basel 486 www.karger.com/cpb Accepted:
(2006) , ; (M OD S) (VA P) ,
(2006) 65 Practical guidelines for m echan ical ven tilation (2006) S ociety of C ritical Care M ed icine Ch inese M ed ical A ssociation Corresp ond ing au thors: Q IN Y ing 2z h i (D ep artm ent of Intensive
(H ipp op hae rham noid es L. ) , ; SHB 2g , 1. 0, 2. 0, 3. 0, 4. 0, 5. 0 ml mm. : Y = X - 0.
34 10 () V o l. 34 N o. 10 2006 10 Jour. of N o rthw est Sci2T ech U niv. of gri. and Fo r. (N aṫ Sci. Ed. ) O cṫ 2006 Ξ 1, 2, 1, 2, 3, 2, 2 (1, 712081; 2, 712100; 3, 123000) [ ],,, : V ( ) V ( 95% ) =
!"!# ""$ %%"" %$" &" %" "!'! " #$!
" "" %%"" %" &" %" " " " % ((((( ((( ((((( " %%%% & ) * ((( "* ( + ) (((( (, (() (((((* ( - )((((( )((((((& + )(((((((((( +. ) ) /(((( +( ),(, ((((((( +, 0 )/ (((((+ ++, ((((() & "( %%%%%%%%%%%%%%%%%%%(
(HR P ) CD 4 + ) L yt2 ( CD 8 + ) F ITC
1999; 17 (6) 325329 Ch inese Journal of Parasito logy and Parasitic D iseases 325 FCC1gHN CSP 3 33 510089 : pcdna 3, (H el a ) (CSP), BALBgc : PfCSP H el a,, EL ISAW estern blotting T N K T, BALBgc : EL
Con struction and D emon stration of an Index System of Know ledge Amoun t of Po sition
2004 9 9 : 00026788 (2004) 0920038209,, (, 20005) :, PAQ, : ; ; ; : C93 : A Con struction and D emon stration of an Index System of Know ledge Amoun t of Po sition DA I Chang2jun, FU L ei, XU H ua (Glo
(TPP)Co 1. 1 ], [ 1, 2 ] V o l. 3 N o. 4 N ov EL ECTROCH EM ISTR Y ) ) ,, Co V V. (T PP) Co (Py) , - 1.
![(TPP)Co 1. 1 ], [ 1, 2 ] V o l. 3 N o. 4 N ov EL ECTROCH EM ISTR Y ) ) ,, Co V V. (T PP) Co (Py) , - 1.](/thumbs/93/113605453.jpg "(TPP)Co 1. 1 ], [ 1, 2 ] V o l. 3 N o. 4 N ov EL ECTROCH EM ISTR Y ) ) ,, Co V V. (T PP) Co (Py) , - 1.")
3 4 1997 11 EL ECTROCH EM ISTR Y V o l 3N o 4 N ov 1997 (TPP)Co a 3 b a a (a 110015) (b 230026) (Py) (T PP)Co 1 22 Py Co (g ) gco (g ) 0 25-0 40 V (vṡ SCE) Py Co (g ) gco (g ) 0 25 V - 0 40 V Py Co (g
Fo recasting Stock M arket Q uo tation s via Fuzzy N eu ral N etw o rk Based on T 2S M odel
2001 2 2 : 100026788 (2001) 0220066207 T 2S,, (, 400044) : T 2S,,, ( ),,. : ; ; α Fo recasting Stock M arket Q uo tation s via Fuzzy N eu ral N etw o rk Based on T 2S M odel CH EN X ing, M EN G W ei2dong,
China B iotechnology, 2005, 25 (12) : pcamb IA21201 DH5 1. 2
China B iotechnology, 2005, 25 (12) : 24 28 A tkup1 3 133 1 2 3 3 (1 150001 2 201106) (3 157011) RNA, RT2PCR,, Km ( ), A tkup1, PCR GUS Southern A tkup1 mrna PCR, PCR 2100bp, A tkup1 ( Genbank No: AF029876)
Error ana lysis of P2wave non2hyperbolic m oveout veloc ity in layered media
28 1 2009 3 Vol128 No11 GLOBAL GEOLOGY Mar1 2009 : 1004 5589 (2009) 01 0098 05 P 1, 1, 2, 1 1., 130026; 2., 100027 :,,,, 1%,,, 12187%,, : ; ; ; : P63114 : A Abstract: Error ana lysis of P2wave non2hyperbolic
BAY ES IAN INFERENCE FO R CO INTEGRATED SY STEM S
997 2 3 JOU RNAL O F SYST EM S EN G IN EER IN G 997 V o l 2 N o 3 (, 300072), :,, : F224 BAY ES IAN INFERENCE FO R CO INTEGRATED SY STEM S Zhag Sh iy ig Zhu Hui Zhag X ibi (Schoo l of M aagem e t T ia
SUPPLEMENTAL INFORMATION. Fully Automated Total Metals and Chromium Speciation Single Platform Introduction System for ICP-MS
Electronic Supplementary Material (ESI) for Journal of Analytical Atomic Spectrometry. This journal is The Royal Society of Chemistry 2018 SUPPLEMENTAL INFORMATION Fully Automated Total Metals and Chromium
1998, 18 (1): 1 7. A cta T heriolog ica S inica (,, ) (Grow th layer group s, GL Gs) 168, 42,
1998, 18 (1): 17 A cta T heriolog ica S inica Ξ (,, 210097), 3,, 3 C (O dum, 1983), 0 2, 24 11 3 R 0 Κ1, rm 0,, ; ; ; ;, (Sh i2 rak ihara, 1993; Gao, 1993)Gao (1993) 3 56,, (, 1995),, 3,, 1974 229, 86,
ZrO 2, ZrO 2. (M ) W O 3g ( 100% ) SO 4 2- W O 3 M oo 3, SO ( Zr (OH ) 4 ) ( ( 0. 1 mo l. L - 1 A gno 3 ), Zr (OH ) 4 SO (0.
14 2 V o l. 14,N o. 2 2000 4 JOU RNAL O F M OL ECULA R CA TAL YS IS (CH INA ) A p r. 2000 : 100123555 (2000) 0220111208 WO 3gZrO 2 - g. ZrO 2,,, 1) (, 130022) : ZrO 2 WO 3gZrO 2 SO 4 2- gzro 2 M oo 3gZrO
Im pact of capac ity a lloca tion on bullwh ip effect in supply cha in
7 4 00 8 JOU RNAL O F SYST EM S EN G IN EER IN G V o l 7 N o 4 A ug 00 ( 30007) : - - - : ; ; ; ; : F5 : A : 000578 (00) 04034009 Im pact of capac ity a lloca tio o bullwh ip effect i supply cha i W AN
FXR1 FM R 1 12 14 15 6 FM R P FXR 1. In teraction s between Six FM RP Isoform s and FXR1 Prote in
14 4 V o l114,n o14 1998 8 Ch inese Jou rnal of B iochem istry and M o lecu lar B io logy A ug 1998 FM RP 3 6 FXR1 A Sittler 3 3 J2L M andel 3 3 (, 100005; 3 3 IGBM C, CN RS2IN SERM 2UL P, 67404 Illk irch,
Ch inese Jou rnal of B iochem istry and M o lecu lar B io logy. E, m RNA T IR
ISSN 100727626 CN 1123870gQ 2001 2 Ch inese Jou rnal of B iochem istry and M o lecu lar B io logy 17 (1): 29 34 m RNA 5 3,, (, 200031) 21 bp B 50 PCNA 2L ac Z m RNA SD 11 bp H ind g, B 50il B 50i2 m RNA,B
Ι ΙΟΤΗΤΕΣ ΤΩΝ ΑΤΟΜΩΝ. Παππάς Χρήστος Επίκουρος Καθηγητής
ΗΛΕΚΤΡΟΝΙΚΗ ΟΜΗ ΚΑΙ Ι ΙΟΤΗΤΕΣ ΤΩΝ ΑΤΟΜΩΝ Παππάς Χρήστος Επίκουρος Καθηγητής ΤΟ ΜΕΓΕΘΟΣ ΤΩΝ ΑΤΟΜΩΝ Ατομική ακτίνα (r) : ½ της απόστασης μεταξύ δύο ομοιοπυρηνικών ατόμων, ενωμένων με απλό ομοιοπολικό δεσμό.
, China Academic Journal Electronic Publishing House. All rights reserved.
488,, 1, 215 10 7 gm l, gl 2 (CPPfgPLLA ) 40%, 72 4, 18 : A, ; B CPPfgPLLA ; C ; D 2 4 6 8 12 16 X A 2, ; 4, ; 8, ; 16,, 43. 6% B C D 3 2 ; 4, ; 16, ; 2,, REPA IR OF UPPER TIB IAL EP IPHY SEAL D EFECT
V isualization of the functional or ien tation column s in the cat v isual cortex by in vivo optical imag ing based on in tr in sic signals
355 : 1 2 1 1 2 3 (11 200433; 21 230027; 31 100101) [ ] [ ] ; ; ; V isualization of the functional or ien tation column s in the cat v isual cortex by in vivo optical imag ing based on in tr in sic signals
Further Study on the Spon taneous Fold ing of Ch icken Apocytochrom e c
15 1 1999 2 V o l 15,N o 1 Ch inese Jou rnal of B iochem istry and M o lecu lar B io logy Feb 1999 3 c 3 3 (, 100101) c V 92A 17, V 92A gc17s W gc17s IA EDAN S A EDAN S2Cys214 A EDAN S T rp 259 V 92A c
ΝΟΜΟΣ ΤΗΣ ΠΕΡΙΟ ΙΚΟΤΗΤΑΣ : Οι ιδιότητες των χηµικών στοιχείων είναι περιοδική συνάρτηση του ατοµικού τους αριθµού.
1. Ο ΠΕΡΙΟ ΙΚΟΣ ΠΙΝΑΚΑΣ Οι άνθρωποι από την φύση τους θέλουν να πετυχαίνουν σπουδαία αποτελέσµατα καταναλώνοντας το λιγότερο δυνατό κόπο και χρόνο. Για το σκοπό αυτό προσπαθούν να οµαδοποιούν τα πράγµατα