Advances in tissue culture of A rnebia euch rom a

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1 Κ2004Κ24; 8ΓΠ A cta B oṫ B orea l. -O cciden ṫ S in. Π ; 2004Γ Ξ 1Κ ; 1 Κ Μ2 Κ Γ Π Κ Κ Κ Π Μ Μ ΠQ ΠA Advances in tissue culture of A rnebia euch rom a X IAO J ie 1Κ2 ΚYAN G Y ing2gen 13 ΚGuo Y i2m ing 1 ΚHUAN G Q in2n i 2 ΚGUO Zhong2chen 1 ; 1 Key L abo rato ry of Pho to synthesis and Environm ental M o lecular Physio logyκinstitute of Bo tanyκch inese A cadem y of Sci2 encesκbeijing ΚChinaΜ2 B io logy D epartm entκcap ital N o rm al U niversityκbeijing ΚChinaΓ AbstractΠA rnebia euch rom a ; Royle. Γ John sṫ is a p lan t w ith w ide u seṡ Sh ikon in derivativesκex tracted from A rnebia euch rom a ; Royle. Γ John sṫ Κw ere ex ten sively app lied in m edical treatm en tκfood and o ther fieldṡ T he am oun t of natu ral A rnebia euch rom a ;Royle. ΓJohn sṫ have sharp ly decreased due to over ex2 p lo itation by hum an beingṡ In recen t yearsκin o rder to so lve the sho rtage of resou rces and to p ro tect envi2 ronm en tκm any researches have been carried ou t in tissue cu ltu re of A rnebia euch rom a ;Royle. ΓJohn sṫ. T he p resen t paper b riefly review ed the studies of the induction and cu ltu re of callu sκcell su spen sion cu ltu re and ferm en tation cu ltu re by b io reacto rκetc. Key wordsπa rnebia euch rom a ;Royle. ΓJohn sṫ Μtissue cu ltu reμp lan tlet regeneration ; A rnebia euch rom a ; Royle. Γ Μ John sṫ ΓΚ Κ m Κ Κ Κ = Ι Π ; sh ikon inγμ ; Γ ; acetylsh ikon inγμβκβ 2 ; ΒΚΒ 2dim ethylacrylsh ikon inγμβ2 ; Β2hydro2xyisovalerylsh ikon inγ Ξ Π Μ Π Π: Φ ; 2001AA Γ Π ; ΓΚ ; ΓΚ Κ Co rrespondence toπyan G Ying2gen. E2m ailπguozyang@public3. bta. neṫ cn

2 8 Κ Π 1561 Κ Κ Κ T abata Κ ;L ithosp erm um ery th rorh iz on Γ [ 1Κ2 ] Κ 1 M SB m ggl NAA m ggl KT [ 3Κ4 ] Κ Κ 25 Κ Μ ph ΚpH 5. 3 [ 3Κ5Κ6 ] ΚpH 5. 8 Μ Κ 2Κ42 Κ D Κ [ 4 ] ; KT 62BA ΓΚ KT 0. 2 m ggl IBA 0. 5 m ggl KT m ggl Κ62BA 0. 6 m ggl IAA KT 0. 4 m ggl NAA [ 3 m ggl 2Κ42D m ggl KT ] [ 9 ] T abata [ 1Κ10 ] IAA m o lgl Κ Μ B 5 N 6 M G5 L S M S M S [ 7 ] ΚL S [ 7 ] [ 6 ] [ 11 ] [ 3Κ4 ] M S m ggl KT + 1 m ggl IAA 15 d ; L S m ggl NAA m ggl 62BA L S m ggl NAA m ggl KT Κ ;L S m ggl IBA m ggl Γ 2 [ 8 ] [ 9 Κ ] Κ Κ Cu 2+ Γ 3 Κ Μ

3 [ 14 Π ] 0 3 d Κ3 12 d Κ12 15 Κ d 0 25 d Κ d Κ [ ] [ 12Κ13 ] 4 Π ; Colletotrichum n icotianceσ F usa rium L S oxy sp orum Σ A sp erg illus n ig erσ Κ M 9 A sp erg illus ory z aeγ 4 ph ph ph Κ ph ;R h iz op us ory z aeσ [ ] ph ph 30% ph Κ Κ Κ BA P NAA IBA 3. 3 Κ2Κ42D Κ 1. 0 m ggl IAA 0. 5 m ggl KT 0. 1 m ggl KT m ggl IAA 10 d 332 m ggl CaC l2 2H 2O Κ Μ m ggl Ca;NO 3Γ2 4H 2O Κ 200 m ggl 10-5 m o lgl L 2 B Κ 10-5 m o lgl B 2Κ Κ Κ 0 3 d Κ3 12 d B 2 Κ12 d 10-7 m o lgl C % ;DM SO Γ+ 5% + 5% Κ 4 Κ 4. 1 [ 17Κ18 ] ; Γ 5 L Κ

4 8 Κ Π 1563 Κ Κ Κ Κ Κ 250 ml 9 [ 19 L 25 L ] [ 22Κ23 ] Κ Κ Κ Κ 9 L Π; 1Γ 0. 4 vvm Κ9 L 2. 3 ggl 25 L gg L 4 ggl 30 L Κ % ; % ΓΚ 30 L Κ Κ ; 2Γ Κ ΚAB 28 Π Κ Κ [ 19Κ20 Κ ] Μ 4. 2 Κ Κ Κ [ 21 ] Μ Κ Κ Ca 2+ 5 Κ Κ Κ Κ Π [ 1 ] TABA TA M ΚM IZU KAM I H ΚH IRAO KA N Κet al. P igm ent fo rm ation in callus calture of L ithosp erm um ery th rorh iz on [J ]. P hy tochem istry Κ 1974Κ13Π

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