(M u ltidrug resistance, M DR )
|
|
|
- Σωτήρης Φωτόπουλος
- 6 χρόνια πριν
- Προβολές:
Transcript
1 V o l. 26 N o CH EM ICAL JOU RNAL O F CH IN ESE UN IV ERS IT IES , 1, 2 (1., ; 2., ) V CR Bel27402 M DR,.,, V CR. Bel27402, V CR, V CR G2, P2170, M DR 1gmRNA, Topo gαmrna. ; ; ; ; R 735 A (2005) (M u ltidrug resistance, M DR ).,,. M DR,,. (P2 ) ; ;, P53 Bcl22; DNA [1 3 ]. M DR,,. T su ruo [4 ],, (Calcium channel b locker, CCB ),., CCB [5 ]. Bel27402,, H ew lett 1100 ; Cou lter Ep ics XL ; M odel 550 (B IO 2RAD ) ; 9700 PCR ; Kodak (EDA S). Bel27402 ( ) ; (BCDC), (V er2 apam il, V R P, ) ; (V incristine, V CR., ) ; P2gp JSB 21 ( ) ; AM V (GibcogBRL ) ; T ripure (GibcogBRL ) ; T aq ( ) ; ( ) M T T 0125%, 10% R PM I gml, 96, : : ( : ) ( : ). : (1962 ),,,,. E2m ail: gaixiaodong@ yahoo. com. cn
2 N o. 8 : 200 ΛL,, 6 : (1) BCDC. 5 ΛL BCDC 300, 320, 340, 350, 360, 380, 390, ΛggmL ; (2) BCDC+ V CR. BCDC 300, 320, 340, 350, 360, 380, 390, ΛggmL, 5 ΛL V CR ( 0102 ΛggmL ) ; (3) V R P+ V CR. V R P 5 ΛL ( 5 ΛggmL ), 5 ΛL V CR ( 0102 ΛggmL ) ; (4), ; (5) V CR. 5 ΛL V CR ( 0102 ΛggmL ) ; (6),. 37 5% CO 2 4 d, 50 ΛL M T T (1 m ggml ), 4 h,, 150 ΛL DM SO, 10 m in, OD 570 nm. : (% ) = OD OD 570 OD OD 570 = [ 6 ], CD I= A B g(a B ) CD I, A B, A B, CD I< 1, Bel27402 V CR [7 ] Bel ml, gml, 24 h, 3 : 110 ΛggmL ) ; (1) Bel V CR ( (2) Bel V CR ( 110 ΛggmL ) + V R P ( 5 Λgg ml ) ; (3) Bel V CR ( 110 ΛggmL ) + BCDC ( 390 ΛggmL ). 24 h, 900 rgm in 10 m in, 019% N ac l 3, 15 ml g, 900 rgm in 7 m in,, 114 ml DDW, , 900 rgm in 30 m in,, 4,. Zo rbax2od S, 0102 mo lgl K 2H PO 4 (ph = 616) 2 ( 20 80), 110 ml g m in, 50, 267 nm Bel gml, 80% 90%, : (1)Bel27402 ; ( 2)Bel V CR ( 0102 ΛggmL ) + V R P ( 5 Λggm l) ; (3) Bel BCDC ( 390 ΛggmL ) ; (4) Bel BCDC ( 390 ΛggmL ) + V CR ( 0102 ΛggmL ). 3,, 900 rgm in 5 m in,, gml, PBS 2, 5 ml 70%,, 4 24 h, rgm in 5 m in,, PBS 2, PBS 1 ml, RN ase, 50 ΛggmL, 37 1 h, (P I), 50 ΛggmL, 4, 30 m in, 200, P2gp S2P P2 (P2gp ) R T 2PCR M DR Bel % R PM I1640, 37 5% CO 2, gml. Bel27402 ; Bel ΛggmL BCDB, 24, 48, h M DR. T rizo l RNA, R T 2PCR, cdna PCR : M DR 1, M R P, GST Π, Topog Α Β2actin 1. : 94 3 m in, s, s, 72 1 m in, m in 30, 115%., M DR 1gΒ2actin, M R PgΒ2actin, GST ΠgΒ2actin Topog gβ2actin, M DR 1, M R P, GST Π Topog ΑmRNA. 1447
3 V o l. 26 Gene M DR1 Table 1 The sequence of pr imers and the length of multiplication products(bp) M ultip lication M ultip lication Sequence of p rim ers Gene Sequence of p rim ers p roduct (bp) p roduct (bp) 5 CCGCT GT TCGT T TCCT T TA GGT GST Π 5 CCGT GGTCTA T T TCCCA GT TCG TCT TCT T T GCTCCTCCA T T GCG3 5 CA GCAA GTCCA GCA GGT T GTA G3 Topog Α 5 GGGT T TA CGA T GAA GA T GT T GGC3 114 Β2A ctin 5 AA GA GA GGCA TCCT GA CCCT M RP 5 GGGTCCCT T T GT T T GT T GTCCG 3 5 A T GCTCA CT T TCT GGCT GGTA G A CCT T GGAA CTCTCT T TCGGCT Bel TA CA T GGCT GGGGT GT T GAA ΛggmL,,, P > 0105; 400 ΛggmL,, P < ΛggmL Bel27402 V CR,, P < 0101, Bel27402, 390 ΛggmL ( 2)., CD I 0189, 390 ΛggmL, 718, V R P A dded arug Cell Contro l V CR V RP+ V CR Table 2 D ose-dependen t exper imen t of BCDC reversal on Bel-7402 Cell L ine b lg(λg ml - 1 ) BCDC c b BCDC+ V CR d d d a. T he date are the m ean values SD, n= 6; b. P < 0101 as compared w ith the contro l cell; c. P < 0105, as compared w ith the con2 tro l cell; d. P < 0101 as compared w ith the cells treated by V CR Bel-7402 VCR, V CR, V CR, V CR m in,., ngg10 ΛL. 390 ΛggmL ( ) nggm 2 ( ) nggm 2, P < 0101, ( ) nggm 2. V CR V CR Bel-7402, (390 ΛggmL ),, V CR G % 37143% (P < 0101), V R P+ V CR G2 (42177% ), ( 3). Bel27402 M DR V CR, V CR,, G2 [8 ]. 10%,, Bel27402 V CR. Group Table 3 The effect of BCDC treatmen t on Cell Cycle a Cell Cycle (% ) G0G1 S G2M Bel Bel V RP+ V CR b Bel ΛggmL BCDC Bel ΛggmL DCDC+ V CR b a. T he cell cycle w as analyzed after 3 days of expo sure to the compounds as described in the experim ental m ethods. A ll data w ere the m ean value SD in trip licate. b. P < 0101 as compared w ith contro l cell group. 1448
![N o. 8 : 2. 4 P-gp P2gp [ 1 (A ) ],, Bel27402 P2gp [ 1 (B ) ]. M DR M DR 1 P2170. P2170,, [9, 10 ]. P2170,., F ig. 1 Expression of P-gp in Bel-7402 cells (A ) Bel27402 cells.](/docs-images/92/108934289/images/4-0.jpg "S2P m ethod 400; (B) Bel27402 cells after BCDC treatm ent fo r 3 d. S2P m ethod 400. 2. 5 4. M DR 1, GST Π Topog Α Bel27402, M R P 30. M DR 1 mrna, ; Topog Α, ; GST Π ( 2).")
4 N o. 8 : 2. 4 P-gp P2gp [ 1 (A ) ],, Bel27402 P2gp [ 1 (B ) ]. M DR M DR 1 P2170. P2170,, [9, 10 ]. P2170,., F ig. 1 Expression of P-gp in Bel-7402 cells (A ) Bel27402 cells. S2P m ethod 400; (B) Bel27402 cells after BCDC treatm ent fo r 3 d. S2P m ethod M DR 1, GST Π Topog Α Bel27402, M R P 30. M DR 1 mrna, ; Topog Α, ; GST Π ( 2). Table 4 The expression changes of mrna related to MD R in Bel-7402 cell treated with DCDC 3 Group T im egd DR 1 GST Π Topog Α Bel Bel BCDC 390 ΛggmL a a a a M ean SD, n= 3; a. P < 0101 as compared w ith contro l cell group at sam e tim e. M DR 1 P2gp,. M R P ABC, M R P [11 ]. GST, GST Π. [12 ], F ig. 2 The level of MD R1, GSTΠ and Topog Α gene transcr ipt in Bel-7402 and Bel-7402 cells treated by 390 ΛggmL BCDC (lane 1, 2, 3, 4, 5, 6 respectively) DNA DNA,. Topog,, DNA 2Topog. Topog, [3 ].., Bel27402 M R P, M DR 1gmRNA P2gp, Topog ΑmRNA, 1449
5 V o l. 26 Topog Α. 3, Bel27402 M DR, Bel27402 M DR 1gmRNA, P2, Topog Α mrna, Bel V CR V CR, V CR G2M.,,. [ 1 ] Endico tt J. A., L ing V.. A nn. Rev. B iochem. [J ], 1989, 58: 137_ 171 [ 2 ] T etu B., B risson J., P lante V. et a l.. M od. Patho l. [J ], 1998, 11 (9) : 823_ 830 [ 3 ] Pomm ier Y., L eteurte F., Fesen M. R. et a l.. Cancer Invesṫ [J ], 1994, 12: [ 4 ] T suruo T., L ida H., T sukago sh i S. et a l.. Cancer Res. [J ], 1981, 41: 1967_ 1972 [ 5 ] GAO X ian2jun ( ). Journal of Integrated T raditional Ch inese and W estern M edicine ( ) [J ], 1990, 10 (7) : 447_ 448 [ 6 ] SU J ian ( ), ZH EN Yong2Su ( ), Q I Chang2Q ing ( ) et al.. A cta Pharm aceutica Sinica ( ) [J ], 1994, 29: 656_ 661 [ 7 ] L I D an2rong ( ), TU W en2sheng ( ), L I L i( ) et al.. Ch inese Journal of Ch rom atography ( ) [J ], 1998, 16 (1) : 50_ 51 [ 8 ] L in H. L., L iu T. Y., W u C. W. et a l.. B r. J. Cancer[J ], 1999, 8 (13) : 416_ 422 [ 9 ] Scam bia G., Ranelletti F. O., Benedetti Panici P. et a l.. Cancer Chemo theṙ Pharmoco l. [J ], 1994, 34: 459_ 464 [ 10 ] L in H. L., L iu T. Y., L uiw. Y. et a l.. Cancer[J ], 1999, 85 (9) : 1937_ 1942 [ 11 ] Jedlitschky G., Kepp ler D.. V itam Ho rm [J ], 2002, 64: 153_ 184 [ 12 ] T ew K. D.. Cancer Res. [J ], 1994, 54: 4313_ 4320 Effect of Bupleurun Ch inese DC (BCDC) on the MD R Reversal of Hepatocellular Carc inoma and Related M echan ism GA I X iao2dong 13, ZEN G Chang2Q ian 1, HON G M in 2 (1. L aboratory of L if e S cience and B iolog ical T echnology, B eihua U niversity, J ilin , Ch ina; 2. M ed icine P rotein and P roteom ics laboratory, J ilin U niversity, Chang chun , Ch ina) Abstract T he reversal effect and m echan ism s of BCDC w ere evaluated by u sing Bel27402 cell line w ith innate resistance in th is study. T he iden tification of new and less tox ic reversal agen t, BCDC, w ou ld open a new perspectives in cancer treatm en ṫ T he resu lts show that BCDC has an effect on M DR reversal of Bel27402 cell line and a synergetic effect w ith V CR. T he reversal m echan ism s w ere due to supp ress M DR 1 tran scrip tion and P2gp exp ression, as a resu lt, to leave TopogΑmRNA level, to increase in tracellu lar V CR concen tration of Bo l27402 cell, to enhance cell G2M phase arrest induced by V CR. Keywords H epatocellu lar carcinom a; M u ltidrug resistance (M DR ) ; M DR reversal; V incristine (V CR ) ; Bup leu run Ch inese DC (Ed. : D, I) 1450
F ig. 1 Flow chart of comprehen sive design in the research of com patibil ity. E2m ail: ac. cn
V o l. 25 N o. 9 2 0 0 4 9 CH EM ICAL JOU RNAL O F CH IN ESE UN IV ERS IT IES 1632 1636,,,, (, 116011) H PL C2PAD 2M S,,.,. H PL C2PAD 2M S.,.,,. 2 ; ; O 652. 7 A 025120790 (2004) 0921632205,,., [1 5 ]
GPS, 0. 5 kg ( In tegrated Fertility Index, IF I) 1. 1 SPSS 10. IF I =
34 11 () V o l. 34 N o. 11 2006 11 Jour. of N o rthw est Sci2T ech U niv. of A gri. and Fo r. (N aṫ Sci. Ed. ) N ov. 2006 α 1, 1, 2, 1 (1, 450002; 2, 410007) [ ]12 () 1 612,, ( IF I ) : (1), ( ) ( ), ph
Supplemental file 3. All 306 mapped IDs collected by IPA program. Supplemental file 6. The functions and main focused genes in each network.
LIST OF SUPPLEMENTAL FILES Supplemental file 1. Primer sets used for qrt-pcr. Supplemental file 2. All 1305 differentially expressed genes. Supplemental file 3. All 306 mapped IDs collected by IPA program.
D NA , ) SCGE DNA.
14 5 V o l114,n o15 1998 10 Ch inese Jou rnal of B iochem istry and M o lecu lar B io logy O cṫ 1998 SCGE D NA (, (, 030006) 030006) (SCGE) ; SCGE Χ DNA, Χ DNA, :,DNA,, Pr inc iple and M ethod of the Single
26 3 V o l. 26 N o A cta Eco logiae A n im alis Dom astici M ay ,
26 3 V o l. 26 N o. 3 2005 5 A cta Eco logiae A n im alis Dom astici M ay 2005,,,,, (, 524088) [ ] 1 200, 3,, 28 31, 83 87%, 1 2 0. 1% 0. 3% : 1 38 47 (P < 0. 05), 48 57 1 (P < 0. 05),,, 1 (P > 0. 05)
Supplementary Table 1. Primers used for RT-qPCR analysis of striatal and nigral tissue.
Supplementary Table 1. Primers used for RT-qPCR analysis of striatal and nigral tissue. Gene Forward Primer (5-3 ) Reverse Primer (5-3 ) Dopaminergic Markers TH CTG GCC ATT GAT GTA CTG GA ACA CAC ATG GGA
(T rip tery g ium w ilf ord ii Hook) ,Beroza [6 ] 4 W ilfo rine W ilfo rdine W ilfo rgine W il2. Euon ine 1. 0% 1980, 1. 1 ; 1. 0%, ; 0.
![(T rip tery g ium w ilf ord ii Hook) ,Beroza [6 ] 4 W ilfo rine W ilfo rdine W ilfo rgine W il2. Euon ine 1. 0% 1980, 1. 1 ; 1. 0%, ; 0.](/thumbs/92/108790990.jpg "(T rip tery g ium w ilf ord ii Hook) ,Beroza [6 ] 4 W ilfo rine W ilfo rdine W ilfo rgine W il2. Euon ine 1. 0% 1980, 1. 1 ; 1. 0%, ; 0.")
34 12 ( ) V o l. 34 N o. 12 2006 12 Jour. of N o rthw est Sci2T ech U niv. of A gri. and Fo r. (N aṫ Sci. Ed. ) D ec. 2006 Ξ 1, 3, 1, 2, 1, 2, 1, 1, 2 (1, 712100; 2, 712100; 3, 450008) [ ], 0. 1%, 3 48
(H ipp op hae rham noid es L. ) , ; SHB 2g , 1. 0, 2. 0, 3. 0, 4. 0, 5. 0 ml mm. : Y = X - 0.
34 10 () V o l. 34 N o. 10 2006 10 Jour. of N o rthw est Sci2T ech U niv. of gri. and Fo r. (N aṫ Sci. Ed. ) O cṫ 2006 Ξ 1, 2, 1, 2, 3, 2, 2 (1, 712081; 2, 712100; 3, 123000) [ ],,, : V ( ) V ( 95% ) =
(P seudo rab ies, PR ) (A u jeszky, s D isease, AD ), (Pesuderab ies viru s, PRV )
34 9 ( ) V o l. 34 N o. 9 2006 9 Jour. of N o rthw est Sci2T ech U niv. of A gri. and Fo r. (N aṫ Sci. Ed. ) Sep. 2006 W G Ξ 1, 1, 1, 1, 1, 2 (1, 712100; 2, 723600) [ ]ge2el ISA 6 187 (P seu2 do rabies
, ( , (P anax qu inquef olius) ) ; L C2V P series U H PE), M Pa R E252. C18 (5 Λm, 250 mm 4. 6 mm ) ( )
150 21 5 2005 5 T ransactions of the CSA E V o l. 21 N o. 5 M ay 2005,,,,, (, 130025) :,, CO 2 : 70%, 200M Pa, 50 1, 2 m in, : ; ; ; : O 62335; TQ 036 +. 4 : A : 100226819 (2005) 0520150205,,,. [J ].,
China Academic Journal Electronic Publishing House. All rights reserved. O ct., 2005
23 10( 142) V ol. 23, N o. 10 200510 System s Engineering O ct., 2005 100124098 (2005) 1020081205 Ξ 1, 2, 1 2, (1., 710049; 21, 266033),,,,,,,, ; ; F224. 32 A 20 6070 [1, 2 ], 1, 1. 1, (1) T = {1, 2},
(g ) A quanau tics,, , 33. 1%. O , O CH EM ICAL JOU RNAL O F CH IN ESE UN IV ERS IT IES (,,, )
V o l. 19 N o. 10 1 9 9 8 10 CH EM ICAL JOU RNAL O F CH IN ESE UN IV ERS IT IES 1584 1588 (g ) (,,, 300071) (, ),,..,, 1. 0 g1,., 33. 1%.,,, O 632. 5, O 629. 7. (H b),., Bonaven tu ra 80, H b, H b 50 100
: H IV. Screen ing of 1F7 Id iotyp ic An ti-gp160 An tibod ies from Phage An tibody L ibrary
14 1 V o l114,n o11 1998 2 Ch inese Jou rnal of B iochem istry and M o lecu lar B io logy Feb 1998 H IV-1 gp 160 2) 1) 1) 1) 3) (, 100850; 2), 100071; 3), 130062) : H IV m RNA, IgG Fd Fab, pcom b3, XL
T IM P-1 cd NA CO S-7. Clon ing of Human T IM P-1 cd NA and its Expression in COS-7 Cells. 21 (tissue in2
ISSN 100727626 CN 1123870gQ 16 3 Ch in. J. B iochem. M o l. B io l. 2000, V o l. 16, N o. 3, 306 311 2000 6 T IM P-1 cd NA 3 CO S-7 3 3 (,,, 100853) GenBank T IM P21, R T 2PCR T IM P21 cdna T 2A pcr R
A ( SPA ) Su lfo succin im idyl 62[ 3 2( 22pyridyldith io ) p rop ionam ido ]hexanoate (Su lfo2l C2SPD P) C E2m ail: w
![A ( SPA ) Su lfo succin im idyl 62[ 3 2( 22pyridyldith io ) p rop ionam ido ]hexanoate (Su lfo2l C2SPD P) C E2m ail: w](/thumbs/91/105112871.jpg "A ( SPA ) Su lfo succin im idyl 62[ 3 2( 22pyridyldith io ) p rop ionam ido ]hexanoate (Su lfo2l C2SPD P) C E2m ail: w")
V o l. 25 N o. 1 2 0 0 4 1 CH EM ICAL JOU RNAL O F CH IN ESE UN IV ERS IT IES 44 49 -C 1, 1, 1, 1, 2, 3 (1., 400038; 2., 400032; 3. 26, 400060) A T 10M H z, C, 2 5 2C.. 215 16010 m IU gl C 01375 1210 nggml,
( , ,
33 9 ( ) V o l. 33 N o. 9 2005 9 Jour. of N o rthw est Sci2T ech U niv. of A gri. and Fo r. (N aṫ Sci. Ed. ) Sep. 2005 Ξ 1, 2, 1, 1, 1, 1, 1, 2 (1 010018; 2 512005) [],, 8 8 9, () (),,,,,, ;, ;,, ;,,,
U (x, y ) = : K (x i- x k) K (x i- x k, y j- y l), 2. 1
22 5 V o l 22, N o 5 2002 9 TR IBOLO GY Sep, 2002,, (, 100084) :, (FFT )., 2 (Green ),,, 3,. ; ; FFT; ; TH 117. 2 A 100420595 (2002) 0520390205,.,,,. L ub rech t [1 ]. (FFT ) [2 5 ]., [4, 5 ].,L iu [6
ZrO 2, ZrO 2. (M ) W O 3g ( 100% ) SO 4 2- W O 3 M oo 3, SO ( Zr (OH ) 4 ) ( ( 0. 1 mo l. L - 1 A gno 3 ), Zr (OH ) 4 SO (0.
14 2 V o l. 14,N o. 2 2000 4 JOU RNAL O F M OL ECULA R CA TAL YS IS (CH INA ) A p r. 2000 : 100123555 (2000) 0220111208 WO 3gZrO 2 - g. ZrO 2,,, 1) (, 130022) : ZrO 2 WO 3gZrO 2 SO 4 2- gzro 2 M oo 3gZrO
, 4, 6, 8 m in; 30%, 50%, 70%, 90% 100% 15, 30, 45, 60, s V c
32 6 ( ) V o l. 32 N o. 6 2004 6 Jour. of N o rthw est Sci2T ech U niv. of A gri. and Fo r. (N aṫ Sci. Ed. ) June 2004 Vc Ξ 1, 2, 2, 1, 3, 3 (1, 712100; 2, 450002; 3, 453003) [ ], V c, V c, V c ; V c,
N-acetyl- l-cyste ine im proves function of islet Β cell in hyperl ip idem ic rats and its m echan ism. V o l 36 N o , NA C PCR
36 6 2007 ( ) JOU RNAL O F ZH EJ IAN G UN IV ERS IT Y (M ED ICAL SC IEN CES) V o l 36 N o 6 2007 h ttp: www. journals. zju. edu. cngm ed Β 1, 1, 1, 1, 1, 1, 2 ( 1. 2., 100029) [ ] : 2N 2 (N 2acetyl2l2cysteine,
Ch in J C lin Tho rac Cardiovasc Surg, A p ril 2008,V o l. 15,No. 2
96 2008 4 15 2 Ch in J C lin Tho rac Cardiovasc Surg, A p ril 2008,V o l. 15,No. 2 1 1 1 2 2 1 1 1 1 (1. ; 21, 650118) : (C IK) (N SCL C) g g g a N SCL C 50,, C IK, 25 ; 2 4 8, CD 3 + CD 4 + CD 4 + gcd
,, 1 mm,, : 250 g, 200 g, 120 g, 50 g 1. 1. 2 50 2 450 g T, 80, , ;,, ,,,,, 1. 2. 1%, 2%, 3% 2005209230 11230, 15 d, 60 d ( ) 1 2 1.
34 6 ( ) V o l. 34 N o. 6 2006 6 Jour. of N o rthw est Sci2T ech U niv. of A ri. and Fo r. (N aṫ Sci. Ed. ) Jun. 2006 Ξ,, (, 712100) [ ] 80 50 T,, 16,,, 1%, 2%, 3%,,,,,, 2% T,LH, FSH (P < 0. 05); T, L
Bank A ssetgl iab ility Sheet w ith Em bedded Op tion s
00 8 8 : 10006788 (00) 08005506, (, 710049) :,, ;, ; : ; ; ; ; ; : F830 : A α Con tro l O ver In terest R ate R isk of Bank A ssetgl iab ility Sheet w ith Em bedded Op tion s LU O D aw ei, W AN D ifang
1998, 18 (1): 1 7. A cta T heriolog ica S inica (,, ) (Grow th layer group s, GL Gs) 168, 42,
1998, 18 (1): 17 A cta T heriolog ica S inica Ξ (,, 210097), 3,, 3 C (O dum, 1983), 0 2, 24 11 3 R 0 Κ1, rm 0,, ; ; ; ;, (Sh i2 rak ihara, 1993; Gao, 1993)Gao (1993) 3 56,, (, 1995),, 3,, 1974 229, 86,
6 cm, 1. 2 IAA, NAA, 2. 1
30 6 ( ) V o l. 30 N o. 6 2002 12 Jour. of N o rthw est Sci2T ech U niv. of A gri. and Fo r. (N aṫ Sci. Ed. ) D ec. 2002 Ξ 1, 2, 2, 3, 1, 4, 1 (1 ; 2 ; 3, 712100; 4, 733200) [],,,,,, ; 1g2 M S, 1g3M S
A R, H ilbert2h uang T ran sfo rm and A R M odel
004 10 10 : 10006788 (004) 1000906 H ilberth uang A R,, (, 41008) : H ilberth uang A R H ilberth uang IM F ( Intrinsic M ode Function), IM F, IM F A R, M ahalanobis,, : H ilberth uang ; ; ; A R ; ; : TH
(A ntifreeze p roteins, A FP s) (afp ),
29 1 ( )V ol. 29 N o. 1 2001 2 Jouṙ of N orthw est Sci2Tech U niv. of A gri. and Foṙ (N aṫ Sci. Ed. ) Feb. 2001 α 1, 1, 2, 3 (1, 712100; 2, 710032; 3, 712100) []A utum n K ing, CTAB DNA, PCR (Polym erase
Quan tif ica tion of Polym era se Cha in Reaction Products Using Solid Pha se Hybr id iza tion-enzym e Color im etr ic D etection
14 2 V o l114,n o12 1998 4 Ch inese Jou rnal of B iochem istry and M o lecu lar B io logy A p ṙ 1998 3 - (, 100044),,,, 125 g, :,,, Quan tif ica tion of Polym era se Cha in Reaction Products Using Solid
1. 2 , N RC (1998) d m g 1
33 2 () V o l. 33 N o. 2 2005 2 Jour. of N o rthw est Sci2T ech U niv. of A gri. and Fo r. (N aṫ Sci. Ed. ) Feb. 2005 Ξ,,,,, (, 450002) [ ] 48, 6, 8, 0, 40, 60, 80, 100 120 ggkg : (1) (P > 0. 05), (P
S ingula r C onfigura tion Ana lys is a nd C oo rd ina te C ontro l of Robo t
36 8 2002 8 JOU RNAL O F SHAN GHA I J IAO TON G UN IV ER S IT Y V o l. 36 N o. 8 A ug. 2002 : 100622467 (2002) 0821138205,,,, (, 200030) : 6R,,.,,. J - 1 (J Jacob ian ). : ; ; ; : T P 391 : A S ingula
; (A con itum kusnez of f ii R eichb. ),.
V o l. 26 N o. 4 2 0 0 5 4 CH EM ICAL JOU RNAL O F CH IN ESE UN IV ERS IT IES 638 641,,, (, 130022) (, 021400)., 4,.,,. ; ; O 657 A 025120790 (2005) 0420638204 (A con itum kusnez of f ii R eichb. ),, [1
The Targeted Express ion of Human IL -2gIFNΑ2b Fused Gene in Hepa toma Cells
15 4 1999 8 V o l 15,N o 4 Ch inese Jou rnal of B iochem istry and M o lecu lar B io logy A ug, 1999 3 IL -2gIFNΑ2b (, 200032), DNA IL 22 IFN Α, A FP galb, EA FP2PALB A, IL 22gIFN Α2b A FP IL 22 IFN IL
Ch in. J. B iochem. M o l. B io l. 2000, V o l. 16, N o. 5, Cd 2+ : Cd 2+ ΛCd 2+
ISSN 100727626 CN 1123870gQ 16 5 Ch in. J. B iochem. M o l. B io l. 2000, V o l. 16, N o. 5, 631 636 2000 10 ΑΑ-cD NA Cd 2+ 3 (,, 100871), Β, Α Α Β, ΑΑ PCR ΑΑ2cDNA A T G AA CA, CaM V 35S ΑΑ2cDNA pgptv
Error ana lysis of P2wave non2hyperbolic m oveout veloc ity in layered media
28 1 2009 3 Vol128 No11 GLOBAL GEOLOGY Mar1 2009 : 1004 5589 (2009) 01 0098 05 P 1, 1, 2, 1 1., 130026; 2., 100027 :,,,, 1%,,, 12187%,, : ; ; ; : P63114 : A Abstract: Error ana lysis of P2wave non2hyperbolic
(H yaluronic acid, HA ) , HA,. HA,, HA. N T G (S trep tococcus zooep id em icus J 18), 50 g
V ol. 25 N o. 5 2 0 0 4 5 CHEM ICAL JOU RNAL O F CH IN ESE UN IV ERS IT IES 853 857 1, 1, 1, 1, 2, 1 (1., 130023; 2., 130022) N 2 2N 2 2N 2 (N TG),., Savage D EA E2 (D E52) Sephadex G275., (S trep tococcus
Fo recasting Stock M arket Q uo tation s via Fuzzy N eu ral N etw o rk Based on T 2S M odel
2001 2 2 : 100026788 (2001) 0220066207 T 2S,, (, 400044) : T 2S,,, ( ),,. : ; ; α Fo recasting Stock M arket Q uo tation s via Fuzzy N eu ral N etw o rk Based on T 2S M odel CH EN X ing, M EN G W ei2dong,
STUD IES ON THE GENOTOX IC ITY OF 83- ITS M AJOR M ETABOL ITE 1 HERB IC ID E AND. H eng Zhengchang 1, T O ng 2
1997 9 3 Carcinogenesis T eratogenesis and M utagenesis V o l 9 N o 3 1997 831 1 T O ng 2 1 610041 2 D ivision of R esp irato ry D isease Studies,N IO SH,U SA Am es V 79 H PR T (SCE) 831 ( ) 2, 4 6 (DCA
PUR IF ICATION AND CHARACTER IZATION OF EXTRACELL ULAR PROTEASE ECPASE 54 FROM A EROM ONAS H YD RO PH ILA
1 9 9 6, 1 9 ( 3 ) : 8 8 9 4 J ou rna l of N anj ing A g ricu ltu ra l U n iversity ECPase 54 Ξ 3 3 (, 210095) (A h) J21 56 (ED TA ) (PM SF), (ECPase), A h J 21 SD S2PA GE, 37,, 5 A h J21 D EA E2 Sephadex
IL -4 . IL 24, cp IL 42PE38KD EL,.
V o l. 26 N o. 5 2 0 0 5 5 CH EM ICAL JOU RNAL O F CH IN ESE UN IV ERS IT IES 874 879 IL -4 1, 2, 3, 1, 2, 1, 2 (1., 110016; 2., 100039; 3. Beckm an R esearch Institute, C ity of Hope N ationalm edical
CHEM ICAL JOU RNAL O F CH IN ESE UN IV ERS IT IES , 20% 30%. . ( A spart pv K100. :
V ol. 25 N o. 6 2 0 0 4 6 CHEM ICAL JOU RNAL O F CH IN ESE UN IV ERS IT IES 10601064 [B10Glu, B24-A sp-b25 ],,, (,, 100871) PCR, B B 10 H is Glu, B 24 B 25 A sp, [B 10Glu, B 242A sp2b 25 ]. pbv 220, DH
1999, 17 (1): J ourna l of W uhan B otan ica l Resea rch ( ) ( ) 2, 3. (Celosia cristata L. ),
1999, 17 (1): 15 20 J ourna l of W uhan B otan ica l Resea rch Ξ ( 210013) (210095),, W HO g FAO, 3, 10, 3 ( ) 2317% 2714%,, 83147% 86194%, (EAA ) 4012% 4117%, (M et+ Cys) 10,,,,,,,,,,, 1 (Celosia cristata
) V o l. 40 N o. 6 : A : Q ,, W ard [ 1 ] (A v icenn ia m a rina) (K and elia cand el)
![) V o l. 40 N o. 6 : A : Q ,, W ard [ 1 ] (A v icenn ia m a rina) (K and elia cand el)](/thumbs/93/114276195.jpg ") V o l. 40 N o. 6 : A : Q ,, W ard [ 1 ] (A v icenn ia m a rina) (K and elia cand el)")
40 6 ( ) V o l 40 N o 6 2001 11 Jou rnal of X iam en U n iversity (N atu ral Science) N ov 2001 : 043820479 (2001) 0621350205,,,, (, 361005) :, (N R ), N R N R, N ac l ; N R : ( 0 30 ; 0 20 )N R, 30, 89
TR IBOLO GY N ov, 2004 , P I2. , 120 M Pa, P I. 6 mm 7 mm 30 mm [7, 8 ] mm,. JSM 25600LV 1. 1 (EDXA ).
![TR IBOLO GY N ov, 2004 , P I2. , 120 M Pa, P I. 6 mm 7 mm 30 mm [7, 8 ] mm,. JSM 25600LV 1. 1 (EDXA ).](/thumbs/50/26571605.jpg "TR IBOLO GY N ov, 2004 , P I2. , 120 M Pa, P I. 6 mm 7 mm 30 mm [7, 8 ] mm,. JSM 25600LV 1. 1 (EDXA ).")
24 6 V o l 24, N o 6 2004 11 TR IBOLO GY N ov, 2004,,, (, 730000) : MM 2200 (PT FE) M os2 (P I) GC r15, X P I, PT FE M os2 P I, P I+ 30%M os2, P I 50%. P I+ 10% PT FE+ 20%M os2, P I, P I+ 20% PT FE+ 10%M
Tsinghua Tongfang Optical Disc Co., Ltd. All rights reserved.
2004 3 4 1 Ch in J Pancreato l, M arch 2004, V o l. 4, N o. 1 5 (severe acute pancreatitis, SA P), SA P 32,, 4 : (N S) (tetrandrine, T et) (ch inese m edicine qing yi tang, CM Q ) T et + CM Q 12 h, 6 h,,
copula, 5 3 Copula Κ L = lim System s Engineering M ay., 2006 : (2006) ,,, copula Ξ A rch im edean copula (Joe,
24 5 ( 149 ) V ol. 24, N o. 5 2006 5 System s Engineering M ay., 2006 : 100124098 (2006) 0520088205 copula Ξ, (, 230052) : (copula), A rch im edean copula, Gum bel2hougard copula,, : Copula; ; : F830 :
(2006) , ; (M OD S) (VA P) ,
(2006) 65 Practical guidelines for m echan ical ven tilation (2006) S ociety of C ritical Care M ed icine Ch inese M ed ical A ssociation Corresp ond ing au thors: Q IN Y ing 2z h i (D ep artm ent of Intensive
D ynam ic changes of som e blood indexes and horm one levels of wa ter buffa loes w ith chron ic F 1hep a tica infection
J ou rnal of N anj ing A g ricu ltu ral U niversity 3 1 1 1 J. Gonzalez2Gallego 2 1 ( 1, 210095; 2, 24071) 8 Do t2el ISA (n= 5) (n= 3) 60, 20 d :, CR 1 2, ; 19,, ; T, B, 22 IgG,, T 3 T 4,, IgG, ; ; ; ;
T he Op tim al L PM Po rtfo lio M odel of H arlow s and Its So lving M ethod
2003 6 6 00026788 (2003) 0620042206 H arlow, 2 3, (., 70049; 2., 7006; 3., 200433) H arlow,,,,, ;, ; ; F832. 5; F830. 9 A T he Op tim al L PM Po rtfo lio M odel of H arlow s ad Its So lvig M ethod W AN
J. Dairy Sci. 93: doi: /jds American Dairy Science Association, 2010.
Supplementary Table 1. Primers and PCR conditions used for the amplification of the goat SCD1 cdna (PCR1 to PCR6) and three SCD1 polymorphic regions (PCR7 to PCR9) PCR Primers Sequence Position 1 Thermal
300071; 3. , ) E2m ail: 263. net CH EM ICAL JOU RNAL O F CH IN ESE UN IV ERS IT IES CH 2CO 2H N H H N
V o l. 2 o. 7 2 0 0 2 7 C EM ICAL JU RAL C I ESE U IV ERS IT IES 299 0 5- --, 2,, 2, (., 00072; 2.,, 0007;., 006) DCC ( ) gb t (2 ), 4 52 22, 52% 8%. M R, P M R, IR. 52 22,., L 260 BEL 740. 52 ; ; ; 626
E scherich ia coli trna L eu. L i Yong L iu W en W ang En2D uo
14 6 V o l114,n o16 1998 12 Ch inese Jou rnal of B iochem istry and M o lecu lar B io logy D ec 1998 trna L eu 3 3 3 3 :,, 34, : 1998202205, : 1998204220 3 3 (, 510275) (, 200031) trna L eu trna L 2 eu
Ch inese Traditional and Herbal D rugs
1215,, DNA H epg2 DNA, (H PCE) H epg2 DNA, H PCE DNA,, 5 m dc,,, DNA 5 m dc,, H epg2 DNA DNA,,,,, DNA, DNA,, CpG, G1,, DNA, : [ 1 ], 1 [J ]1, 2005 (2) : 332361 [ 2 ] Slow S, L ever M, L ee M B, et al.
Ch inese Jou rnal of B iochem istry and M o lecu lar B io logy. (o steoarth ritis, OA )
ISSN 100727626 CN 1123870gQ 2001 4 Ch inese Jou rnal of B iochem istry and M o lecu lar B io logy 17 (2): 244 249 1) 1), 3, 2), 1), 1), 1), 2) 2), ( 1), 710032; 2), 100044) ( rheum ato id arth ritis, RA
V o l. 53, N o. 3 M ay, 1998 A CTA GEO GRA PH ICA S IN ICA ) m 3 gs,
53 3 1998 5 A CTA GEO GRA PH ICA S IN ICA V o l. 53, N o. 3 M ay, 1998 (, 11201), 1,,,, [1, 2 ] 60 00 m 3 gs, 266 300 m 3 gs, 01226 8 [2 ],,, 2 A B ( 1a),,, 1a E F, E F, CN F ( 1b), 1a A CN F 1b A Cf,
A Knowledge M odel for D esign of Population Nutr ien t Index D ynam ics in W heat
2005, 25 (3): 47 52 Journal of T riticeae C rop s Ξ,,,, ( g,, 210095) :,,,,, ( ) 2,, RM S E 8. 13 kgghm 2, RM S E 0. 20%, : ; ; ; ; : S 512. 1; S 311 : A : 100921041 (2005) 0320047206 A Knowledge M odel
CHEM ICAL JOU RNAL O F CH IN ESE UN IV ERS IT IES [1, 2 ]. ., FT IR, , g ( cm - 1 ). H 2O cm - 1. :
![CHEM ICAL JOU RNAL O F CH IN ESE UN IV ERS IT IES [1, 2 ]. ., FT IR, , g ( cm - 1 ). H 2O cm - 1. :](/thumbs/93/112604099.jpg "CHEM ICAL JOU RNAL O F CH IN ESE UN IV ERS IT IES [1, 2 ]. ., FT IR, , g ( cm - 1 ). H 2O cm - 1. :")
V ol. 24 N o. 2 2 0 0 3 2 CHEM ICAL JOU RNAL O F CH IN ESE UN IV ERS IT IES 226 231, (, 100875) BSA R ase A, : 1 330 1 290 cm - 1 Α2 ; 1 295 1 265 cm - 1 Β2 ; 1 270 1 245 cm - 1 ; 1 250 1 220 cm - 1 Β2.,,
,,,. , 1. 2 1. 1. 1 BM B 005 ( ] 1. 1. 2 , SJM - IM L - 90 ( V ellank [ 15 ] 1. 2. 1 1. 5 2. 0 2. 5 3. 0. 49. 7 58. 5 67. 3 76. 1 84.
![,,,. , 1. 2 1. 1. 1 BM B 005 ( ] 1. 1. 2 , SJM - IM L - 90 ( V ellank [ 15 ] 1. 2. 1 1. 5 2. 0 2. 5 3. 0. 49. 7 58. 5 67. 3 76. 1 84.](/thumbs/41/22710127.jpg ",,,. , 1. 2 1. 1. 1 BM B 005 ( ] 1. 1. 2 , SJM - IM L - 90 ( V ellank [ 15 ] 1. 2. 1 1. 5 2. 0 2. 5 3. 0. 49. 7 58. 5 67. 3 76. 1 84.")
21 2 2005 2 T ransactions of the CSA E V o l. 21 N o. 2 Feb. 2005 25,,, (,,, 430070) : 30 75 750 rgm in,,,, A rrhenius,, : ; ; ; : Q 81 : A : 100226819 (2005) 0220025205,,,. [J ]., 2005, 21 (2): 25-29.
DY NAM IC CHANGE OF EP ID ERM AL STEM CELL S IN THE WOUND HEAL ING COURSE OF D IABETIC RATSg
693 (diabetes m ellitus, DM ) (ep iderm al stem cells, ESC s), ESC s DM 48 W istar,, 180 200 g DM, 24 DM DM 115 cm 118 cm 2154 cm 2 ( 96 ), 3 7 14 21, ;, H E 19 (keratin 19, K19) Β1, 3 7 14 21, 24148%
The Changes of Card iova scular Respon se to O rthosta tic Stress Caused by Hypovolem ia Inuced by W e ightlessness: A Sim ula tion Study
J B iom ed Eng 2002; 19 (1) 48 52 3 1 2 1 2 2 1 ( 100084) 2 ( 710032) M elch io r (1994) ( ) 15% ; 5% LBN P HR BP The Changes of Card iova scular Respon se to O rthosta tic Stress Caused by Hypovolem ia
(TPP)Co 1. 1 ], [ 1, 2 ] V o l. 3 N o. 4 N ov EL ECTROCH EM ISTR Y ) ) ,, Co V V. (T PP) Co (Py) , - 1.
![(TPP)Co 1. 1 ], [ 1, 2 ] V o l. 3 N o. 4 N ov EL ECTROCH EM ISTR Y ) ) ,, Co V V. (T PP) Co (Py) , - 1.](/thumbs/93/113605453.jpg "(TPP)Co 1. 1 ], [ 1, 2 ] V o l. 3 N o. 4 N ov EL ECTROCH EM ISTR Y ) ) ,, Co V V. (T PP) Co (Py) , - 1.")
3 4 1997 11 EL ECTROCH EM ISTR Y V o l 3N o 4 N ov 1997 (TPP)Co a 3 b a a (a 110015) (b 230026) (Py) (T PP)Co 1 22 Py Co (g ) gco (g ) 0 25-0 40 V (vṡ SCE) Py Co (g ) gco (g ) 0 25 V - 0 40 V Py Co (g
Το άτομο του Υδρογόνου
Το άτομο του Υδρογόνου Δυναμικό Coulomb Εξίσωση Schrödinger h e (, r, ) (, r, ) E (, r, ) m ψ θφ r ψ θφ = ψ θφ Συνθήκες ψ(, r θφ, ) = πεπερασμένη ψ( r ) = 0 ψ(, r θφ, ) =ψ(, r θφ+, ) π Επιτρεπτές ενέργειες
5 Ι ^ο 3 X X X. go > 'α. ο. o f Ο > = S 3. > 3 w»a. *= < ^> ^ o,2 l g f ^ 2-3 ο. χ χ. > ω. m > ο ο ο - * * ^r 2 =>^ 3^ =5 b Ο? UJ. > ο ο.
728!. -θ-cr " -;. '. UW -,2 =*- Os Os rsi Tf co co Os r4 Ι. C Ι m. Ι? U Ι. Ι os ν ) ϋ. Q- o,2 l g f 2-2 CT= ν**? 1? «δ - * * 5 Ι -ΐ j s a* " 'g cn" w *" " 1 cog 'S=o " 1= 2 5 ν s/ O / 0Q Ε!θ Ρ h o."o.
BAY ES IAN INFERENCE FO R CO INTEGRATED SY STEM S
997 2 3 JOU RNAL O F SYST EM S EN G IN EER IN G 997 V o l 2 N o 3 (, 300072), :,, : F224 BAY ES IAN INFERENCE FO R CO INTEGRATED SY STEM S Zhag Sh iy ig Zhu Hui Zhag X ibi (Schoo l of M aagem e t T ia
CHEM ICAL JOU RNAL O F CH IN ESE UN IV ERS IT IES , ( ECV 304) (angiogenesis),, :
V ol. 24 o. 2 2 0 0 3 2 CHEM ICAL JOU RAL O F CH I ESE U IV ERS IT IES 260 264 1, 3- -1, 3- -2H- 1, 2, 2, 2 (1. ; 2., 610041) ΑvΒ3 R GD,, 5 52 21, 32 21, 32 22H 2,, ( ECV 304) R GD ; ; 1, 32 21, 32 22H
Con struction and D emon stration of an Index System of Know ledge Amoun t of Po sition
2004 9 9 : 00026788 (2004) 0920038209,, (, 20005) :, PAQ, : ; ; ; : C93 : A Con struction and D emon stration of an Index System of Know ledge Amoun t of Po sition DA I Chang2jun, FU L ei, XU H ua (Glo
Ch in. J. B iochem. M o l. B io l. 2000, V o l. 16, N o. 6,
ISSN 100727626 CN 1123870gQ 16 6 Ch in. J. B iochem. M o l. B io l. 2000, V o l. 16, N o. 6, 814 819 2000 12 3 RNA 3 3 (, 200032) upa R RNA pu RA S M DA 2M B 2231, G418 N o rthern upa R RNA, R T 2PCR upa
60 s 72, 60 s 94, 30 s; 5
342 Ch inese Journal of Parasito logy and Parasitic D iseases 3 510515 :, CS : PCR CS, M 13, 3 M 132CSP DNA, PCGEN E CS : (PFD - 3gYN ) T 4 W ellcom e N F54 3D 7 7G8 CS, P. f T hgt c : CS (circum spo rozo
Expression of Functiona l D oma in g of Ca lpa sta tin in E. coli and Prepara tion of An tiserum Aga in st Pur if ied GST-Ca lpa sta tin
ISSN 100727626 CN 1123870gQ 16 1 Ch in. J. B iochem. M o l. B io l. 2000, V o l. 16, N o. 1, 23 27 2000 2 g 3 1) 2) 2) 1) ( 1), 2), 100094) PCR (calpastatin) cdna g (404 bp ), pgex2kg pgex2calp 4 IPT G
(classical sw ine fever, CSF) (CSFV ) (O IE) A 15, 1999202212 96,, C2,, 20 70 80, 1. 1 SXZZ), 1. 2
31 5 () V o l. 31 N o. 5 2003 10 Jour. of N o rthw est Sci2T ech U niv. of A gri. and Fo r. (N aṫ Sci. Ed. ) O cṫ 2003 E2 gp55 Ξ 1, 1, 1, 1, 1, 2, 2 (1, 712100; 2, 730046) [ ] R T 2PCR PCR E 2,, A lfo
17 1 V o l. 17 N o CH IN ESE JOU RNAL O F COM PU TA T IONAL M ECHAN ICS February 2000 : A
7 V o l. 7 N o. 000 CH IN ESE JOU RNAL O F COM PU TA T IONAL M ECHAN ICS February 000 : 0074708 (000) 000808 Ξ, (, 0098) :,,,,,,, N ew tonr aph son,, : ; ; ; : TU 3933; O 48 : A 80, [, Geiger Fu ller ],
Ch in. J. B iochem. M o l. B io l. 2000, V o l. 16, N o. 2, K 3 (A 6D )
ISSN 100727626 CN 1123870gQ 16 2 Ch in. J. B iochem. M o l. B io l. 2000, V o l. 16, N o. 2, 234239 2000 4 (P I4-K) 3 1) (,, 100101; 1), 100871) (P I 42K) (A 6D ) 2, 71510 6 610 8 (m o lgl ) - 1 11910-7
acrysta ll iferous stra in Btk BE20
J ou rnal of N anj ing A g ricu ltu ral U niversity S18-4 - Btk BE20 3 1 2 2 2 2 ( 1, 321004; 2, 510275) S1824, PH T 3101, DNA P stl, B tk BE 20 SD S2PA GE, 2,, S1824 ; 2 ; ; S48217 Clon ing and expression
Μελέτη της έκφρασης του ογκοκατασταλτικού γονιδίου Cyld στον καρκίνο του μαστού
Σχολή Θετικών Επιστημών Τμήμα Βιολογίας Πρόγραμμα Μεταπτυχιακών Σπουδών Κατεύθυνση: Εφαρμοσμένη γενετική και βιοτεχνολογία ΜΕΤΑΠΤΥΧΙΑΚΗ ΔΙΠΛΩΜΑΤΙΚΗ ΕΡΓΑΣΙΑ Μελέτη της έκφρασης του ογκοκατασταλτικού γονιδίου
Jour. of N o rthw est Sci2T ech U niv. of A gri. and Fo r. (N aṫ Sci. Ed. ) Sep , 1 [7 ] ,, 2 ,, 1 85% [1 4 ], [ ]
![Jour. of N o rthw est Sci2T ech U niv. of A gri. and Fo r. (N aṫ Sci. Ed. ) Sep , 1 [7 ] ,, 2 ,, 1 85% [1 4 ], [ ]](/thumbs/49/25047659.jpg "Jour. of N o rthw est Sci2T ech U niv. of A gri. and Fo r. (N aṫ Sci. Ed. ) Sep , 1 [7 ] ,, 2 ,, 1 85% [1 4 ], [ ]")
33 9 () V o l. 33 N o. 9 2005 9 Jour. of N o rthw est Sci2T ech U niv. of A gri. and Fo r. (N aṫ Sci. Ed. ) Sep. 2005 60 Co Χ Ξ,,, (, 712100) [ ] 3 60 Co Χ, 3,, 60 Co Χ, Χ, ; 60 Co Χ,,,, ;, 60 Co Χ, 3,
(diam inobenzidine,dab ) ; 0. 5 mmo lgl, : N ac l 8 g, KC l 0. 2 g T ris 3
34 8 ( ) V o l. 34 N o. 8 2006 8 Jour. of N o rthw est Sci2T ech U niv. of A gri. and Fo r. (N aṫ Sci. Ed. ) A ug. 2006 Ξ 1, 2, 1 (1, 712100; 2, 100850) [ ] g,,, 85%,,, 8 ; 6. 5 10 5 g; Fas2L, 95% ;, [
M elittin,. G IGAVL KVL T T GL PAL ISW IKR KRQQ N H 2. M elittin 5, 4 C (KR KR ). M elittin, 1. 1
V ol. 24 N o. 3 2 0 0 3 3 CHEM ICAL JOU RNAL O F CH IN ESE UN IV ERS IT IES 449 453 1, 1, 1, 1, 2 (1.,, 300071; 2. ALBA CH EM L im ited & the Edinburgh Centre for P rotein Technology, D epartm ent of Chem
G IS N N E E, , km 2, 92% A CTA GEO GRA PH ICA S IN ICA
55 4 2000 7 A CTA GEO GRA PH ICA S IN ICA V o l. 55, N o. 4 July, 2000 : 037525444 (2000) 0420407210 G IS,, (, 100101) : G IS, 1982 1997 ;, : ; ; ; G IS; : F301124; F29312; N 94511 : A 20 80 90, [1, 2
A (GPA ), (Perco ll) A, Gene Express ion of Erythrocyte M em brane Glycophor in A in N IDDM
14 5 V o l114,n o15 1998 10 Ch inese Jou rnal of B iochem istry and M o lecu lar B io logy O cṫ 1998 g GPA 3 (, 410078) g (N IDDM ) A (GPA ), (Perco ll) 15 25 N IDDM, RNA, N o rthern GPA GP W estern, N
CH EM ICAL JOU RNAL O F CH IN ESE UN IV ERS IT IES nm, nm nm,,,. [1, 2 ] :
![CH EM ICAL JOU RNAL O F CH IN ESE UN IV ERS IT IES nm, nm nm,,,. [1, 2 ] :](/thumbs/48/24815001.jpg "CH EM ICAL JOU RNAL O F CH IN ESE UN IV ERS IT IES nm, nm nm,,,. [1, 2 ] :")
V o l. 26 N o. 4 2 0 0 5 4 CH EM ICAL JOU RNAL O F CH IN ESE UN IV ERS IT IES 623 627 1, 2, 1, 2, 1, 1 1, 1, 1, 2 (1., 116023; 2., 100039),, 10 20 nm, 20 40 nm 40 60 nm,,,.,,.,, ; Χ Α, Χ.. ; ; ; ; O 641
Parts Manual. Trio Mobile Surgery Platform. Model 1033
Trio Mobile Surgery Platform Model 1033 Parts Manual For parts or technical assistance: Pour pièces de service ou assistance technique : Für Teile oder technische Unterstützung Anruf: Voor delen of technische
M athem aticalm odel and A lgo rithm of In telligen t T est Paper
2004 9 9 : 100026788 (2004) 0920085205,,,, (, 210016) :,,,, : ; ; ; ; : T P319 : A M athem aticalm odel and A lgo rithm of In telligen t T est Paper A u to2generation System from Item Poo l W AN G You2ren,
The p re pa ra tion a nd a ntiba c te ria l a c tiv ity in vitro of be rbe rine hyd rochlo ric na nom e te r m ic roem uls ion
35 1 () V o l. 35 N o. 1 2007 1 Journal of N o rthw est A & F U niversity (N aṫ Sci. Ed. ) Jan. 2007 Ξ, (, 712100) [ ]4 ( IPM ) 3 (EL 40 Tw een280) ;,,,,, ( ), EL 402 2IPM ;, 56. 8 nm ;, ;, [ ]; ; ; ;
Effects of PD on IL -6- induced Growth Arrest and Term ina l D ifferen tia tion of M 1 Cells
ISSN 100727626 CN 1123870gQ 2001 2 Ch inese Jou rnal of B iochem istry and M o lecu lar B io logy 17 (1): 115 120 PD 098059 IL -6 M 1, 3,,, (, 100850) IL 26 M 1 R asgm A PK, M EK PD 098059 R asgm A PK,
Rub isco. Rubisco. R ubisco R ubisco , R ubisco
(Ch inese J R ice S ci) 2001 15 (1) : 35 40 Rub isco Rub isco ( 310029) R ub isco A ctivase and Its R egu lation on D iu rnal Changes of Pho to syn thetic R ate and the A ctivity of R ibu lo se 1 52B ispho
F ingerpr in ts of X in shu Ora l L iquid by HPCE
839 1, 1, 2, 2 1Ξ, (11, 110015; 21, 110016) : 10, 10, 10, 10,,,,,,, 27 14, 10 ( 7 ), 9 : ; ; : R 283; R 286102 : A : 0253 2670 (2006) 06 0839 05 F ingerpr in ts of X in shu Ora l L iquid by HPCE GUO T
E62 AC Series Features
E62 AC Features Perfect for non-sinusoidal s and pulsed s. Housed in a hermetically sealed aluminum can which is filled with environmentally friendly plant oil as standard. The integrated overpressure
V irus ide ntifica tion a nd sc re e ning v irus ic ide s of pe ppe r v irus d ise a se in S ha a nxi P rov ince
35 1 () V o l. 35 N o. 1 2007 1 Journal of N o rthw est A & F U niversity (N aṫ Sci. Ed. ) Jan. 2007 Ξ 1, 2, 2, 1, 1 (1, 712100; 2, 710003) [] 126, 5, DA S2EL ISA,, (CM V ) (TM V ) (T EV ) Y (PV Y) (BBWV
IRBB 4 (Xa24) IRBB 7 (Xa27) IRBB 21 (Xa221) 6
J ou rna l of N anj in ricu ltu ra l U n iversity Ξ F 1 (, 210095) 12 7 10 : Xa24 xa25 Xa27 xa213 Xa221 5 3 I 4 (Xa24) I 7 (Xa27) I 21 (Xa221) 6 5, F 1 KS266 ZJ2173, Xa27 Xa221 F 1, Xa24 F 1 Xa27 Xa221
, E, PRO GR ESS IN GEO GRA PH Y. V o l122, N o15 Sep t1, 2003 : (2003) , 263, (0 1m ) P93511; P42616
22 5 2003 9 PRO GR ESS IN GEO GRA PH Y V o l122, N o15 Sep t1, 2003 : 100726301 (2003) 0520454209 1, 1, 2, 3 (11 100875; 21, 100877; 31 100875) : 1982 1998 AV HRR pathfinder, 29 263, (0 1m ), 1982 1998
Jou rnal of M athem atical Study
38 4 2005 12 Jou rnal of M athem atical Study V ol 38 o 4 D ec 2005 α (, 361005),, (SVV ), SVV, SVV, SVV - H elm holtz, (SEM ); (SVV ); O 174 52 A 1,, 1g, ( ),, 80,, ( [ 4 ]),,,, (e g [ 1 ]),, Gibbs,,,,,,
ο ο 3 α. 3"* > ω > d καΐ 'Ενορία όλις ή Χώρί ^ 3 < KN < ^ < 13 > ο_ Μ ^~~ > > > > > Ο to X Η > ο_ ο Ο,2 Σχέδι Γλεγμα Ο Σ Ο Ζ < o w *< Χ χ Χ Χ < < < Ο
18 ρ * -sf. NO 1 D... 1: - ( ΰ ΐ - ι- *- 2 - UN _ ί=. r t ' \0 y «. _,2. "* co Ι». =; F S " 5 D 0 g H ', ( co* 5. «ΰ ' δ". o θ * * "ΰ 2 Ι o * "- 1 W co o -o1= to»g ι. *ΰ * Ε fc ΰ Ι.. L j to. Ι Q_ " 'T
(2006) ,A RD S. AL Ig A RD S. AL IgA RD S A RD S A RD S A RD S , A RD S 25% 50%, 11% 25%, 9% 26% 1 AL IgARD S , A RD S 50% 6815%
706 g (2006) Guidel ines for managem en t of acute lung in jurygacute resp ira tory d istress syndrom e: an ev idence- ba sed update by the Ch inese Soc iety of Cr itical Care M edic ine (2006) Ch inese
D ecision2m ak ing M odel Inco rpo rating R isk Behavio r under P ro ject R isk M anagem en t
2001 10 10 : 100026788 (2001) 1020001208 2 1 2, (1., 210096 2. ) : : : TU 72 : A α D ecision2m ak ing M odel Inco rpo rating R isk Behavio r under P ro ject R isk M anagem en t L I Q i2m ing 1, SH EN L
Νόµοςπεριοδικότητας του Moseley:Η χηµική συµπεριφορά (οι ιδιότητες) των στοιχείων είναι περιοδική συνάρτηση του ατοµικού τους αριθµού.
Νόµοςπεριοδικότητας του Moseley:Η χηµική συµπεριφορά (οι ιδιότητες) των στοιχείων είναι περιοδική συνάρτηση του ατοµικού τους αριθµού. Περιοδικός πίνακας: α. Είναι µια ταξινόµηση των στοιχείων κατά αύξοντα
CHEM ICAL JOU RNAL O F CH IN ESE UN IV ERS IT IES V (vṡ [1, 2 ]. OH OH :
![CHEM ICAL JOU RNAL O F CH IN ESE UN IV ERS IT IES V (vṡ [1, 2 ]. OH OH :](/thumbs/92/109606076.jpg "CHEM ICAL JOU RNAL O F CH IN ESE UN IV ERS IT IES V (vṡ [1, 2 ]. OH OH :")
V ol. 23 N o. 6 2 0 0 2 6 CHEM ICAL JOU RNAL O F CH IN ESE UN IV ERS IT IES 1151 1155,,,, (, 430072) ( ),., T io 2, 0. 05 V (vṡ SCE),, H 2O 2,,. ; ; T io 2 ; ; O 644. 14 A 025120790 (2002) 0621151205,
Expression and Pur if ica tion of W ild and M utan t Type NAD H-Cytochrom e b5 Reducta se in E. coli
ISSN 100727626 CN 1123870gQ 16 4 Ch in. J. B iochem. M o l. B io l. 2000, V o l. 16, N o. 4, 452 457 2000 8 NAD H- b5 (, 350025) NADH 2 b5, (b5r ), (C203Y) b5r cdna pgex22t, BL 21 W estern GST 2b5R 2Sepharo
( ) Jou rnal of Fudan U n iversity (N atu ral Science) , 1964 ],, 56 ( ) [ 5 ]. 1998 7,,, ( 15
![( ) Jou rnal of Fudan U n iversity (N atu ral Science) , 1964 ],, 56 ( ) [ 5 ]. 1998 7,,, ( 15](/thumbs/41/22742618.jpg "( ) Jou rnal of Fudan U n iversity (N atu ral Science) , 1964 ],, 56 ( ) [ 5 ]. 1998 7,,, ( 15")
38 5 199910 ( ) Jou rnal of Fudan U n iversity (N atu ral Science) V o l 38 N o 5 O ct 1999 : 042727104 (1999) 0520517206 α 1, 1, 1, 2, 2 3, (1 ; 2 ; 3 ) : 200 ;, (T FRC), a2b (a2brc), atd, (tpd ),,,,,,
Ch in. J. B iochem. M o l. B io l. 2000, V o l. 16, N o. 3, U CP. (P < 0101) ; U CP 2 m RNA , R T 2PCR
ISSN 100727626 CN 1123870gQ 16 3 Ch in. J. B iochem. M o l. B io l. 2000, V o l. 16, N o. 3, 394 399 2000 6 3-1 2 3 3 3 (,, 100730) 21 2 3 (U CP1, 2, 3), R T 2PCR U CP 1, 2, 3 m RNA U CP 1. U CP 2, 3,
OPN antisense oligodeoxynucleotide inhibits p roliferation and apop tosis of human breast carcinoma cell line
2009 8 29 8 Basic & C linical M edicine August 2009 Vol. 29 No. 8 : 1 00 1 263 25 ( 2 0 0 9 ) 0 8 20 8 5 0 20 5 13, 2, 3, 4, 5 (11, 730030; 21, 730000; 31, 730000; 41, 730000; 51, 730020) : (OPN ) mrna
, ( CO 2+ O 2+ N 2, ], N 2 [ 14, 15 ] O 2. ( 0. 1 ml ) ; (23 Jayasingh h atm ) (2001) [ 7 ]
![, ( CO 2+ O 2+ N 2, ], N 2 [ 14, 15 ] O 2. ( 0. 1 ml ) ; (23 Jayasingh h atm ) (2001) [ 7 ]](/thumbs/49/25044241.jpg ", ( CO 2+ O 2+ N 2, ], N 2 [ 14, 15 ] O 2. ( 0. 1 ml ) ; (23 Jayasingh h atm ) (2001) [ 7 ]")
156 19 3 2003 5 T ransactions of the CSA E V o l. 19 N o. 3 M ay 2003 1 2 2 2 (1. 030801; 2. 100094) : CO + CO 2+ N 2 O 2+ CO 2+ N 2 O 2+ CO 2+ N 2 (4 1) 21 d (ph TVB2N TBA ) : 1)CO 2M A P CO 2M A P 21