F ingerpr in ts of X in shu Ora l L iquid by HPCE
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1 839 1, 1, 2, 2 1Ξ, (11, ; 21, ) : 10, 10, 10, 10,,,,,,, 27 14, 10 ( 7 ), 9 : ; ; : R 283; R : A : (2006) F ingerpr in ts of X in shu Ora l L iquid by HPCE GUO T ao 1, SU I Y in 1, SUN Y i 2, L I Fa2m ei 2, GAO Sheng2chuan 1 (11 D epartm ent of Pharm acy, T he General Ho sp ital of Shenyang M ilitary R egion, Shenyang , Ch ina; 21 Co llege of Pharm acy, Shenyang Pharm aceu tical U n iversity, Shenyang , Ch ina) Abstract: Objective To develop a m ethod of quality con tro l fo r fingerp rin ts of X in shu O ral L iqu id1 M ethods Based on electropho regram of ten batches of genu ine R ad ix A ng elicae S inensis (RA S), ten batches of genu ine R h iz om a Chuanx iong (RC), and ten batches of genu ine F los Ca rtham i (FC ) by h igh perfo rm ance cap illary electropho resis (H PCE) to com pare the fingerp rin ts betw een X in shu O rall iqu id and the genu ine m edicinal herb s, single herb decoction, nagetive con tro l herb so lu tion, respectively1 T he fin2 gerp rin t assignm en t w as m ade by com paring the UV spectra and relative m igration tim e1 Results To com pare the fingerp rin ts of ten sam p les from differen t batches and single herb, the co rrelation of peak s be2 tw een fingerp rings w as found1 F inally the standard fingerp rin ts and the m ethod of quality con tro l w ere estab lished1 Conclusion Based on the fingerp rin ts of ten batches of p rearation s, an average electro2pho re2 gram w as u sed as the standard fingerp rin t1 T here are 27 comm on peak s in the fingerp rin t, am ong them 14 from RA S, ten from RC (in w h ich seven are comm non) and n ine from FC1 Key words: X in shu O ral L iqu id; fingerp rin ts; h igh perfo rm ance cap illary electropho resis (H PCE) H P 3D G1600A ( A gilen t, ) ; A S5150A ( ) ;, (, ), 10 ( ) 10 (, ) 10 ( ), 10, ; ( ), ( ) ( ) ; (10 ml g, 10 ) Ξ :
2 840 Ch inese Traditional and Herbal D rugs : 50 mm o lgl, 10 mm o lgl, 2% (ph 916), 24 kv 40 mm o lgl, 40 mm o lgl, 5% (ph 819), 24 kv 50 mm o lg L, 18% (ph 917), 24 kv 105 mm o lgl (ph 917), 0 30 m in kv, m in 28 kv 210 nm, 5 kpa 5 s, m o lgl m in, F ig11 Electrophoregram s of reference substances 8 m in [ 1 3 ] 212 : m g 40 m g 40 m g, 60%, 50 ml, 0145 Λm, A : 12B 12ferulic acid 22adeno sine 32Pentobarbital sodium 42R ifamp icin 52rutin 62quercetin, 0145 Λm,,, 213 : 2 2 (A) (B) (C) F ig12 3D UV Electrophorogram of extracts of RAS (A), RC (B), and FC (C) : 5,,, h, R SD R SD 110%, 018%, R SD 416% R SD 415%, R SD 113%, 24 h R SD 512% :, R SD 116%, R SD 0145 Λm, 3 612% 215 : : 1,, [ 4, 5 ] h,, R SD 019%, R SD 411% 10, 27 R SD 019%,, R SD 419% 13, 18, 27 R SD 112%, R SD % , 1, 2
3 841 3 (A) (B) (C) (D ) (E) (F) (G) F ig13 Electrophorogram s of X in shu Oral liquid (A), decoction s of RAS (B), RC decoction (C), FC decoction (D ), negative sam ples without RAS (E), without RC (F), and without FC (G) 1 10 Table 1 Relative m igration tim e of f ingerpr in t for ten batches of X in shu Oral L iquid RSD g% , , 6, 17 12, 16, 19, 10, 10 10, 17 10, 27 4, 11 13, 18, 27,
4 842 Ch inese Traditional and Herbal D rugs Table 2 Relative peak area of f ingerpr in t for ten batches of X in shu Oral L iquid RSD g% ,,, 10,, 24, 3, 2 3 ; Table 3 Recogn itive of correlative peak in f ingerpr in ts, between X in shu Oral L iquid and single herbs m in, 45 m in, 45 m in , 17, 2 ;, m in, 40 m in, 40 m in , ,, 3 ;
5 843, References: m in, 50 m in, 50 m in 314, ), 2003, 23 (5) : 3481,, , (14 ), (10 ), (9 ) [ 1 ] Chen Y, Cheng Z Y, H an F M, et al1 Q uantitative analysis of ferulic acid content in D anggui by h igh perfo rm ance cap illary electropho resis [J ]1 Ch in J A nal Chem ( ), 1999, 27 (12) : [ 2 ] Guo T, Sun Y, Sui Y, et al1 D eterm ination of ferulic acid adeno sine in A ngelica sinensis by m licellar electrokinetic cap il2 lary ch rom atography [J ]1 Ch in J P harm A nal ( [ 3 ] Sun Y, Guo T, Sui Y, et al1 D eterm ination of adeno sine, rutin and quercetin in Cartham us tinctorius by H PCE [J ]1 A cta P harm S in ( ), 2003, 38 (4) : 2831 [ 4 ] W ang L X, X iao H B, L iang X M, et al1 V ecto rial angle m ethod fo r evaluating the sim ilarity betw een two ch rom a2 to2 graphic fingerp rints of Chinese herb1 [J ]1 A cta P harm S in ( ), 2002, 37 (9) : [ 5 ] W ang X H, A n R, Zou Y, et al1 A pp lication of ch rom ato2 graph2digitized fingerp rint spectrum in identification of m edici2 nal m aterials and qualitative contro l of Shuang2 huanglian p reparation [J ]1 Ch in T rad it H erb D rug s ( ), 2002, 33 (2) : , 3 Ξ,,,, (, ) :, ; 2H PL C,, 1 3, ;, ; 98 nm, ( ) % (n= 3) : ; ; ; : R : A : (2006) Prepara tion of brev iscap ine proliposom e by f luid ized bed coa ting m ethod L E I Guo2feng, D EN G Y ing2jie, ZHAN G L i2na, ZHON G H ai2jun, L I Zhe, ZHAN G Yong (Schoo l of Pharm acy, Shenyang Pharm aceu tical U n iversity, Shenyang , Ch ina) Abstract: Objective To enhance the o ral b ioavailab ility of b reviscap ine and investigate the feasib ility of p reparing the p ro lipo som e of b reviscap ine by flu idized bed coating m ethod1m ethods T he flu idized bed coating m ethod w as u sed to p repare b reviscap ine p ro lipo som e and u ltrafiltration2h PL C w as app lied to de2 term ination of the encap su lation efficiency1 T he influence facto rs on encap su lation efficiency including car2 riers, drug2lip id w eigh t ratio, in let air tem peratu re, sp ray rate, and sp ray air vo lum e w ere investigated1 Results W hen the carrier w as so rb ito l and the drug2lip id w eigh t ratio w as under 1 3, the encap su lation efficiency cou ld be h igher1 In let air tem peratu re, sp ray rate, and sp ray air vo lum e had som e effects on the encap su lation efficiency1 By the m ethod, the flu idity of the p ro lipo som e granu les w as w ell and the particle size distribu tion ratio of them w as un ifo rm ; the m ean particle size of lipo som al fo rm u lation of b reviscap ine after rehydration w as 98 nm, and the encap su lation efficiency w as ( ) % (n= 3) 1 Conclusion It is feasib le to p repare the b reviscap ine p ro lipo som e w ith flu idized bed coating m ethod1 Key words: b reviscap ine; p ro lipo som e; flu idized bed; encap su lation efficiency :
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