) V o l. 40 N o. 6 : A : Q ,, W ard [ 1 ] (A v icenn ia m a rina) (K and elia cand el)

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1 40 6 ( ) V o l 40 N o Jou rnal of X iam en U n iversity (N atu ral Science) N ov 2001 : (2001) ,,,, (, ) :, (N R ), N R N R, N ac l ; N R : ( 0 30 ; 0 20 )N R, 30, 89 5%, 20 N R 192%,N R :, : ; ; ; ; : Q : A,,,,,, (N R ), N R,, W ard [ 1 ] N R, N R T ischner [ 2 ] Stoh r [ 3 ] N R ; Chen [ 4 ] [ 5 ] N R (A v icenn ia m a rina) (K and elia cand el), N R, N R, : : ( ) : (1977- ),, : China Academic Journal Electronic Publishing House All rights reserved

2 6 : (24 29 N, E),, 35 cm 14 cm,, g, 10 6, ( ) 500 ml, 0 20 d, 60 d,, N ac l 1 2 : Sandeliu s &M o rre [ 6 ] Zheng [ 7 ],,,, 1 2 (W gv ) ED TA 2 N a2 3 mm o lgl, Sucro se 0 25 mm o lgl, PV P K %, PM SF 1 mm o lgl, T ris2m es 15 mm o lgl (ph 7 8), m in, 240 Λm, g 15 m in, g 30 m in,, N R 5 mm o lgl (ph 7 8), D T T 0 1 mm o lgl, KC l 1 mm o lgl 1 3 (V gv ) D ex tran T %, PEG %, Sucro se 0 25 m o lgl, KC l 3 mm o lgl, K 3PO 4 5 mm o lgl, 20 30, g,,, g 15 m in,, T ris2m es 5 mm o lgl (ph 7 2), D T T 0 1 mm o lgl, Sucro se 0 25 m o lgl 3 5, g 30 m in, T ris2m es 5 mm o lgl (ph 7 8), D T T 0 1 mm o lgl, Sucro se 0 25 m o lgl, 0 4 : W idell [ 8 ] (N a3vo 4) K + 2A T Pase ph 6 5 N a3vo 4 : B randfo rd [ 9 ], G N R N R T ab 1 Comparison of activity of N R betw een cytop lasm and p lasm a m em brane fo r A m arina N ac l N R N R gmmo ll - 1 gλmo l NO 2 - m g - 1 p ro m in - 1 gλmo l NO 2 - m g - 1 p ro m in (265% ) (218% ) (247% ) (252% ) (153% ) China Academic Journal Electronic Publishing House All rights reserved

3 1352 ( ) 2001 N R : Scho ll [ 10 ] slam [ 11 ] NO 2 -, 1 ml : 25 mm o lgl (ph 7 5), KNO 3 10 mm o lg L, T riton X %,NADH 0 5 mm o lgl, 50Λg m in, 17 mm o lgl ( = 3 1 ) 1 ml 7 mm o lgl Α2 ( = 3 1 ) 1 ml, 15 m in, OD 530 NO 2 - NO , 90% K + 2A T Pase ( ),, K + 2A T 2 Pase N a3vo 4 91%, 2 2 N R N R 1 1 (Am ) (Kc) ( ) N R N R, F ig 1 Effects of salinity on N R activity in leaf p lasm a m em branes fo r A m arina and K cand el seedlings N R, N R N R N ac l, 525 mm o lgl, N R N R, 240%,,,, N R, ; N R [ 12, ] China Academic Journal Electronic Publishing House All rights reserved

4 6 : N R 1 N R N R,, N R, 30, N R 89 5% ; 20,N R 192% 30 ( ) 20 ( ), N R, N R, N R,, [ 5, N R ], N R N R N R, N R,,, 30 ( ) 20 ( ), :,,,, 3 1) N R, N R N R 2) N R N R N ac l 3) N R,, 30, 20 : [1 ] W ard M R, Gim es H D, H uffaker R C L atent nitrate reductase activity is associated w ith the p lasm a m em brane of co rn roo ts[j ] P lanta, 1989, 177: [ 2 ] T ishner R, W ardm R, H uffaker R C Evidence fo r a p lasm a m em brane- bound nitrate reductase in in2 vo lved in nitrate up take of Ch lo rella so rok iniana[j ] P lanta, 1989, 178: [3 ] Stoh r C, T ishner R, W ard M R, et al Evidence fo r a p lasm a m em berane2bound nitrate reductase in Ch lo rella saccharoph ila[j ] N adson P lan ta, 1993, 191: [4 ] Chen J, F ischer E, L uttge U Berich t der Bo tanikertagung R egensburg[m ] In: Schaeffer J C (Ed), Berlin: Sp ringer V erlag, H eidlberg, [ 5 ],,, [J ], 1995, 37 (12): [6 ] Sandelius A S, M o rre D J P lasm a m em brane iso lation [M ] In: L arsson C, M o ller IM (Eds): T he p lant p lasm a m em brane Berlin: Sp ringer V erlag, [ 7 ] Zheng H L, Zhao Z Q, Zhang C G, et al Changes in lip id peroxidation, the redox system and A T Pase activities in p lasm a m em branes of rice seedling roo ts caused by lanthanum ch lo ride [J ] 2000, 13: B iom etals, China Academic Journal Electronic Publishing House All rights reserved

5 1354 ( ) 2001 [ 8 ] W idell S, L arsson C A critical evaluation of m arkers used in p lasm a m em brane purification [M ] In: L arsson C, M o ller IM (Eds): T he P lant P lasm a M em brane Berlin: Sp ringer V erlag, [9 ] B randfo rdm M A rap id and sensitive m ethod fo r quantitation of m icrogram quantities of p ro tein utiliz2 ing the p rincip les of p ro tein dye binding[j ] A nal B iochem, 1976, 72: [ 10 ] Scho ll R L, H arper J E, H agem an R H, et al Imp rovem ents of the nitrate co lo r developm ent in as2 says of n itrate reductase by phenazine m etho su lfate and zinc acetate[j ] P lan t PH ysio l, 1974, 53: [11 ] A slam M, Ro sichan J L, H uffaker R C Comparative induction of nitrate reductase by nitrate and ni2 trite in barley leaves[j ] P lan t Physio l, 1987, 83: [12 ], [J ], 1994, 24 (10): Effect of Salin ity on the A ctivity of N itrate R eductase in P lasm a M em b rane of L eaves fo r A m a rina and K cand el Seedlings ZHAO Zhong2qiu, ZH EN G H ai2lei, ZHAN G Chun2guang,M A J ian2hua, L IN Peng (Schoo l of L ife Sci, X iam en U n iv, X iam en , Ch ina) Abstract: N itrate reductase (N R ) activity w as iden tified in the p lasm a m em b rane vesicles of leaves fo r and K cand el seedlings by tw o2phase partition ing In A m a rina, the activity of N R w as m uch low er than that in cytop lasm, bu t they w ere bo th affected by N ac l concen tra2 tion A nd the activity of N R in the p lasm a m em b rane of leaves fo r A m a rina and K cand el seedlings w as also affected by cu ltu re salin ity: N R activity increased w ith in certain range of cu ltu re salin ity (A m a rina: 0 30 ; K cand el: 0 20 ), w h ile h igher salin ity inh ib ited its activity T he op tim al salin ity w as 30 fo r A m a rina and 20 fo r K cand el, In com pari2 son, K cand el w as m o re sen sitive to salin ity than A m a rina Key words: A m a rina; K cand el; salin ity; p lasm a m em b rane; N R China Academic Journal Electronic Publishing House All rights reserved