METABOLIC ENGINEERING OF IMPORTANT COMPONENTS OF PLANT SEEDS

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1 2006,20 (2) : Journal of Nuclear Agricultural Sciences 123 : (2006) (, ) : ( ) : ; ; ; ; METABOLIC ENGINEERING OF IMPORTANT COMPONENTS OF PLANT SEEDS CHEN Jin2qing WU Guan2ting ( Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou, Zhejiang ) Abstract :Advances in metabolic engineering of important components of plant seeds, tubers and fruits including starch, sugar, protein, lipid, and other nutrient substances were briefly reviewed in this paper. Key words :seed composition ; starch ; protein ; lipid ; metabolic engineering ( ) ( ),,,,,,,,,,, ( ) 1,,, ADP2 (AGP) ( SS) (SBE) (DBE), AGP 212 ADP2,, SS 2 1,4, SS SBE DBE,AGP CaMV 35S AGP cdna, AGP 2 %, 3. 5 % 5 % ;,,, 30 % 8 % [1 ] Lloyd AGP,,, : : ( ) (M303144) : (19532),,,,, org

2 [2 ] Weber AGP cdna B4, ( Vicia narbonensis), AGP 95 %,, 18 % 35 %, 12 % 25 % [3 ], AGP,, AGP, AGP, 60 % [4 ] AGP,, (SS), ( GBSS) waxy GBSS, [5,6 ], GBSS, 70 % 100 % [7 ] [17 ] Terada 213kb Wx cdna, 2 [8 ], Wx, AW621 13, B,, Wx [9,10 ] (SBE) Schwall 2 SBE, SBE 1 %,,, 5 [11 ] Weber B4, (apoplast),, 50 % 80 %, , , [12 ],, [13 ] 21,4 6 8, [14 ] 2,,, 2, 2 4 % [15,16 ],, 20 %, [18 ] 3 [19 ], 2 [20 ] Penarrubia,,, [21 ],, Tada kda,, [22 ] (HMW), HMW, HMW, HMW, HMW,

3 2 125 [23 28 ] 8,, ( Corynebacterium) (DHDPS) dapa lysc ( AK, ),, [29,30 ],Tu [31 ] (PEPCase), ( PEP), PEP ACCase ACC1 napin, T 1 ACCase 10 20, 5 % [33 ] (LPAAT) 3, LPAAT,, LPAAT, 1315 % [34 ] 312 ( ) ( ) ( ) 3, ACP Knutzon ACP, 2 % 40 %, 20 [35 ],,,,,, 92, ( ) 62 2 ( ) 2 Mart n 32 cdna, RNA 2 60 % [36 ], 85 % [37 ] Fad2, 1 2 [32 ], % 25 %, [38 ], %, 16 18, , A (ACCase), ACCase, 12 02ACP, A A, [39,40 ], Ch FatB1 Ch FatB2, Ch FatB [41 ], 11 % 27 %, 2 CoA KCS1 FA E1, [42,43 ] 4,

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