Progress in Studies of Collagen

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1 ISSN CN ΠQ Chinese Journal of Biochemistry and Molecular Biology 17 (6) : ( ) (collagen ) C Q249 Progress in Studies of Collagen CHEN Ting LU Jun ZHANGJing LI Yu2xin 3 HUANG Bai2qu ( Institute of Genetics and Cytology Northeast Normal University Changchun China) Abstract Collagen a new member of the collagen family has been classified as the subclass which col2 lagen belongs to It plays an important role in adhering basement membrane to the underlying connective tissue stroma Some results suggest that it is involved in the invasive process of carcinomas and it may provide a sensitive indicator of tumor invasion Recently restin a C2terminal fragment of the collagen has been reported as an angiogenesis inhibitor which shares homology with endostatin The gene structure and organiza2 tion tissue distribution and the activity of collagen were discussed Key words collagen molecular structure gene location tissue distribution bioactivity Gly2X2Y (collagens forming sheets) [1 ] Gly2X2Y 3 1 ( ) 2 ( ) 3 ( ) ( ) ( beaded fila2 [2 ] ments) [3 19 ] (anchoring fibrils) (protein with multiple triple2helix domains and interrup2 tions multiplexins) [5 ] : : (973 ) (No G ) 3 Tel : (0431) Fax : (0431) [4 ] (fibril associated collagens with interrupted tri2 Received :April ;Accepted :July Supported by a Major State Basic Research Program ( 973 Program ) Fund ple helices FACITs) (No G ) Partially 3 Corresponding author Tel : (0431) Fax : (0431)

2 SDS2PAGE 1 [7 ] cdna 45 % [6 ] 1388 ( Fig ( 1) 9 1) N [7 ] COL1 [9 ] C (NC1) ( thrombospondin NC10 NC11 [8 ] [ ] (COL1 COL9) 8 16 % (NC2 NC9) % 61 % [10 ] 183 [ ] C 256 [11 (NC10) 4 ] NC10 NC11 Cys [7 ] ( Fig 1) [9 ] NC1 motifs) 2 Cys ( ) 11 2Ser2Gly ( Fig 1) 46 % [9 Kivirikko ( 1) 91 ] kd [7 ] Myers N2 6 ( 1) 125 kd [6 ] Kivirikko 8 [8 ] Fig 1 The molecular structure of human collagen and mouse collagen (A) Human collagen ; (B) Mouse collagen Signal piptide ; collagen domain ; non2collagen domain ; tsp :thrombospondin 2 55 kb [12 ] kb kb N bp 548 bp 89 bp 55 kb 55 kb 12 36

3 6 : 677 [14 8 ] bp 1 54 bp 2 63 bp 1 81 bp C [15 N C ] Eklund [12 ] Hagg 3 + 1A [ ] TT [12 ] TATAA ATG 4 SP1 G+ C(68 4 %) Huebner [13 9q212q22 ] 21 21q22 3 [14 10 ] 3 [15 16 ] [20 Kivirikko ] C NC11 Northern [21 ; ] mrna [21 ] ; Lewis mrna [16 ] Hagg [22 ] 1 C [17 ] NC10 C NC11 NC10 C [20 ] Col15 ( 1) [19 ] 412 [4 ] : 1999 John

4 [11 ] Western blot C (NC10) (related to endostatin restin) Ram2 chandran res2 tin kd restin restin 30 % restin [23 ] 2000 Sasaki restin NC10 VEGF FGF22 NC10 FGF22 restin NC10 NC10 ( ) [10 ] ( References) 1 Eastoe J E Composition of collagen and allied proteins In :Ramachan2 dran G N ed Treatise on CollagenLondon : Academic Van der Rest M Garrone R Collagen family of proteins FAS EB J : Steinert P M Roop D R Molecular and cellular biology of intermediate filaments Annu Rev Biochem : Rehn M Pihlajaniemi T 1 ( ) a collagen chain with frequent in2 terruptions in the collagenous sequence a distinct tissue distribution and homology with type collagen Proc Natl Acad Sci USA : Oh S P Kamagata Y Muragaki Y Timmons S Ooshima A Olsen B R Isolation and sequencing of cdnas for proteins with multiple domains of Gly2Xaa2Yaa repeats identify a distinct family of collagenous proteins Proc Natl Acad Sci USA : Myers J C Kivirikko S Gordon M K Dion A S Pinhlajaniemi T Identification of a previously unknown human collagen chain 1 ( ) characterized by extensive interruptions in the triple2helical region Proc Natl Acad Sci USA : Kivirikko S Heinamaki P Rehn M Honkanen N Myers J C Pihla2 janiemi T Primary structure of the 1 chain of human type collagen and exon2intron organization in the 3 region of the corresponding gene J Biol Chem (7) : Muragaki Y Abe N Ninomiya YOlsen B R Ooshima A The human 1 ( ) collagen chain contains a large amino2terminal non2triple heli2 cal domain with a tandem repeat structure and homology to 1 ( ) collagen J Biol Chem (6) : Rehn M Hintikka E Pihlajaniemi T Primary structure of the 1 chain of mouse type collagen partial structure of the corresponding gene and comparision of the 1 ( ) chain with its homologue the 1 ( ) collagen chain J Biol Chem (19) : Sasaki T Larsson H Tisi D Claesson - Welsh L Hohenester E Tim2 pl R Endostatins derived from collagens and differ in struc2 tural and binding properties tissue distribution and anti2angiogenic ac2 tivity J Mol Biol (5) : John H Preissner K T Forssmann W G Standker L Novel glycosylat2 ed forms of human plasma endostatin and circulating endostaitin2related fragments of collagen Biochemistry (32) : Hagg P M Muona A Lietard J Kivirikko S Pihlajaniemi T Complete exon2intron organization of the human gene for the 1 chain of type collagen ( COL15A1) and comparison with the homologous COL18 1 Gene J Biol Chem (28) : Huebner K Cannizaro L A Jabs E W Kivirikko S Manzone H Pihlajaniemi T Myers J C Chromosomal assignment of a gene encoding a new collagen type (COL15A1) to 9q21 q22 Genomics : 14 Oh S P Warman M L Seldin M F Cheng S D Knoll J M Timmons S Olsen B R Cloning of cdna and genomic DNA encoding human type collagen and localization of the 1 ( ) collagen gene to mouse chromosome 10 and human chromosome 21 Genomics (3) : Myers J C Dion A S Abraham V Amenta P S Type collagen exhibits a widespread distribution in human tissues but a distinct local2 ization in basement membrane zones Cell Tissue Res (3) : Kivirikko S Saarela J Myers J C Autio2Harmainen H Pihlajaniemi T Distribution of type collagen transcripts in human tissue and their production by muscle cells and fibroblasts Am J Pathol (5) : Hagg P M Hagg P O Peltonen S Autio2Harmainen H Pihlajaniemi T Location of type collagen in human tissue and its accumulation in the interstitial matrix of the fibrotic kidney Am J Pathol (6) : Eklund L Piuhola J Komulainen J Sormunen R Ongvarrasopone C Fassler R Muona A Ilves M Ruskoaho H Takala T E S Pihlajanie2 mi T Lack of type collagen causes a skeletal myopathy and cardio2

5 6 : 679 vascular defects in mice Proc Natl Acad Sci USA (3) : Amenta P S Briggs K Xu KGamboa E Jukkola A F Li D Myers J C Type collagen in human colonic adenocarcinomas has a different distribution than other basement membrane zone proteins Hum Pathol (3) : Hanabal M D Volk R Ramchandran R Simons M Sukhatme V P Cloning expression and in vitro activity of human endostatin Biochem Biophys Res Commun : O Reilly M S Boehm T Shing Y Fukai N Vasios G Lane W S Flynn E Birkhead J R Olsen B R Folkmen J Endostatin an endoge2 nous inhibitor of angiogenesis and tumor growth Cell 1997b 88 (2) : Boehm T Folkman J Browder T O Reilly M S Antiangiogenic thera2 py of experimental cancer does not induce acquired drug resistance Nature (6658) : Ramchandran R Dhanabal M Volk R Waterman MJ F Segal M Lu H Knebelmann B Sukhatme V P Antiangiogenic activity of restin NC10 domain of human collagen :Comparison to endostatin Biochem Bio2 phys Res Commun : : : : : : ( ) : : ( ) : ( ) : : 3 : 3 : 3 : 3 : ( ) : 320 :200031