HDL HDL S1P. Micro Channel array Flow Analyzer (MC-FAN) ex vivo S1P S1P HDL MC-FAN HDL HDL S1P. Owen HDL S1P S1P HDL ELISA M (1) mean ±S.D.

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2 HDL HDL - S1P S1P S1P β HDL β HDL HDL MM S1P Owen 2 M HDLM S1P S1P S1P M HDL HDLM S1P ELISA M HDL S1P M HDL (1) M HDLM HDLM M HDL 75gOGTT M 75gOGTT M HDL LDL CRP C- HbA1c 75gOGTT 75gOGTT M M M HDL LDL CRPC- HbA1c Micro Channel array Flow Analyzer (MC-FAN) ex vivo M MC-FAN 75gOGTT 50 75gOGTT (2) HDL S1P S1P HDL HDL S1P M S1P (1) mean ±S.D ± 2.7 HbA1c 5.25±0.21 % C- 90.9±6.6 mg/dl 6.9±7.4IU/L 1.45 ± 0.69ng/ml (TC) 185.6±28.90 mg/dl LDL (LDL-C) 101.4±26.86 mg/dl HDL (HDL-C)64.74±14.19 mg/dl (TG)78.52±58.66 mg/dl 75gOGTT (IGT) 1 (IFG) 470 I IV 4 I:30 II: 60 III: 120 IV: I:20 (4.3%) II:84 (17.9%) III:124 (26.4%) IV:226 (48.1%) HbA1c HOMA-R

3 HDL-C 105 M TC HDL 75gOGTT Matsuda index M gOGTT 7-8, III IV HDL-C, HDL-C HDL-C,, M Matsuda index M MC-FAN M HOMA-IR TG McAuley HOMA-IR McAuley Hct Hb TG HDL-C LDL-C /HDL-C BMI HOMA-IR McAuley Hct Hb WBC Plt LDL-C/HDL-C BMI Hct WBC M TC HDL-C LDL-C PT Hct M TC HDL-C LDL-C PT M M M gOGTT % 362 I~IV I:2 (0.5%) II:25 (6.9%) III:45 (12.4%) IV:290 (80.1%) III IV I II 3 80% III IV Disposition Index 222 M Matsuda index M Matsuda index 20 III+IV 74.5% % III+IV HDL-C M Matsuda index

4 M 75gOGTT (2) MIN6 HDL S1P βtc HDL HDL 75gOGTT M M HDL-C TC 75gOGTT Matsuda Index M 1 7 Yoshida K, Kimura T, Aoki T, Tsunekawa K, Araki O, Shoho Y, Nara M, Sumino H and Murakami M. Fasting serum insulin levels and insulin resistance are associated with blood rheology in Japanese young adults without diabetes. J Int Med Res Jun;44(3): ( ) Yoshida K, Kimura T, Shimizu T, Sato K, Aoki T, Sumino H, Tsunekawa K, Miyazaki O, Fukamachi I, Nakajima K, Okajima F, Murakami M. Serum apolipoprotein M levels are correlated with biomarkers of coagulation. The KITAKANTO Medical Journal 2016 Jun;44(3): doi: / ( ) Yagi H, Sumino H, Yoshida K, Aoki T, Tsunekawa K, Araki O, Kimura T, Nara M, Nakajima K, Murakami M. Biological Antioxidant Potential Negatively Correlates with Carotid Artery Intima-MediaThickness. International Heart Journal 2016 Mar 22;57(2): doi: /ihj ( ) Yagi H, Sumino H, Aoki T, Tsunekawa K, Araki O, Kimura T, Nara M, Ogiwara T, Murakami M. Impaired blood rheology is associated with endothelial dysfunction in patients with coronary risk factors. Clin Hemorheol Microcirc. 2016;62(2): doi: /CH ( ) Aoki T, Tsunekawa K, Araki O, Ogiwara T, Nara M, Sumino H, Kimura T, Murakami M. Type 2 Iodothyronine Deiodinase Activity is Required for Rapid Stimulation of PI3K by Thyroxine in Human Umbilical Vein Endothelial Cells. Endocrinology. 2015;156(11): ( ) Aoki T, Yagi H, Sumino H, Tsunekawa K, Araki O, Kimura T, Nara M, Ogiwara T, Nakajima K, Murakami M. Relationship between carotid artery intima-media thickness and small dense low-density lipoprotein cholesterol concentrations measured by

5 homogenous assay in Japanese subjects. Clin Chim Acta Mar 10;442: doi: /j.cca Epub 2015 Jan 17. ( ) Tsunekawa K, Yanagawa Y, Aoki T, Morimura T, Araki O, Kimura T, Ogiwara T, Kotajima N, Yanagawa M, Murakami M. Frequency and clinical implication of the R450H mutation in the thyrotropin receptor gene in the Japanese population detected by Smart Amplification Process 2. Biomed Res Int. 2014;2014: doi: /2014/ Epub 2014 May 5. ( ) 7 Kamiyama K, Kimura T, Machida T, Murakami M. The sodium glucose co-transporter 2 receptor inhibitors, the newest class of drugs for type 2 diabetes, attenuated the results of leukocytes tested by urine dipsticks. The 32nd World Congress of the International Federation of Biomedical Laboratory Science Aoki T, Kimura T, Tsunekawa K, Araki O, Nara M, Yoshida A, Machida T, Murakami M. Assessment of the glucose tolerance among young Japanese subjects - by the shape of plasma glucose concentration curve during OGTT - The 32nd World Congress of the International Federation of Biomedical Laboratory Science Kimura T, Tsunekawa K, Aoki T, Shimizu T, Miyazaki O, Fukamachi I, Sumino H, Nakajima K, Okajima F, Murakami M. Clinical significance of apolipoprotein M: apolipoprotein M may be a biomarker of coagulation and fibrinolysis. The 9th Cherry Blossom Symposium International Conference of Clinical Laboratory Automation and Robotics ( ) Tsunekawa K, Aoki T, Araki O, Ogiwara T, Kimura T, Nara M, Sumino H, Machida T, Murakami M. Association between the effect of physical exercise in glucose matabolism and the polymorphism of type 2 iodothyronine deiodinase. The 9th Cherry Blossom Symposium International Conference of Clinical Laboratory Automation and Robotics ( ) Aoki T, Kimura T, Simizu T, Sumino H, Miyazaki O, Fukamachi I, Nakajima K, Okajima F, Murakami M. Clinical implication of aporipoprotein M: apolipoprotein M may be a biomarker of coagulation and fibrinolysis. 82th European Atherosclerosis Society Congress (Madrid ) ,,,,,,,, M M

6 (1) KIMURA Takao (2) (3) (4)

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