coronary heart disease CHD 40% [1] atherosclero- sis AS 52.27±5.3 2~6. / polycyclic aromatic hydrocarbons PAHs

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1 AhR rs AS-PCR AhR rs AhR rs χ 2 AhR rs OR 95%CI AhR rs AA AG GG χ 2 =6.48 P<0.05 G A AG GG OR %CI 0.88~ %CI 1.22~5.50 AhR rs G AhR R589.2 A DOI: /j.cnki.issn coronary heart disease CHD AhR 40% [1] 1 [2] 1.1 atherosclero sis AS [3] ±5.3 [ 4] 1 2 [5] 2~6 / 3 4 polycyclic aromatic hydrocarbons PAHs ±5.2 [6] PAHs aryl hydro- 1 2 carbon receptor AhR α 3 LXRα 4 [7] AhR rs [8-10] / B AhR AhR rs hyjiao1602@hotmail.com DNA 3mL

2. rs2066853 141 Na2EDTA - 2A 2B 3 DNA TNE -20 DNA 5μL DNA 195μL 40 260nm 280nm OD OD260/OD280 1.8~2.0 DNA 1.2.2 Tag 2a 2b AG 3a 3b GG 10 Buffer Mg 2+ dntps DNA Maker 1 AhR rs2066853 AS-PCR Gold view 1.

2 2. rs Na2EDTA - 2A 2B 3 DNA TNE -20 DNA 5μL DNA 195μL nm 280nm OD OD260/OD ~2.0 DNA Tag 2a 2b AG 3a 3b GG 10 Buffer Mg 2+ dntps DNA Maker 1 AhR rs AS-PCR Gold view 1.3 SPSS 17.0 Tris Gene Amp PCR System 9700 Gel Doc2000 χ 2 P 0.05 BIO-RAD 2 BIO-RAD 2.1 Hardy-Weinberg Primer 5.0 AhR rs Hardy -Weinberg χ 2 =0.28 P > AhR rs μmol μl F1 5 -GGCATTGAT TTTGAAGACATCAG-3 F2 5 -GGCATTGATTT TGAAGACATCAA-3 χ 2 =6.48 P<0.05 A G χ 2 = μL 10 buffer P>0.05 AhR rs ag Mg 2+ dntps Tag GG DNA 5 1 AA μL :94 OR %CI: 0.88~2.18 5min 95 35s s %CI: 1.22~5.50 1min min PCR 1.5% OR %CI 0.81~ bp AS-PCR rs PCR 1 3 AS-PCR DNA AS-PCR PCR 2 DNA 1 SNPs n=205 AhR rs % AhR rs A/A Marker 500/400/300/200/100/25bp 1a 1b AA AhR rs AA AG GG n=190 χ 2 P OR 95%CI A/G ~ <0.05 G/G ~5.50 A/G+G/G ~

3 AhR rs % SNPs n=205 n=190 χ 2 P OR 95%CI AhR rs A G > ~2.05 AhR rs G [11] AhR AhR AhR 7p15 AhR 1 National Health and Family Planning Commission. 50kb China Statistical Yearbook of Health and Family Planning M. Beijing: Beijing Union Medical University 96kDa /Per-Arnt- Press Sim AhR 2 Finegold JA Asaria P Francis DP. Mortality from ischaemic heart disease by country region and age: [12] AhR -p - tetrachlorodibenzo-p-dioxin TCDD Nations J. Int J Cardiol : statistics from World Health Organisation and United AhR 3 Shah S Casas JP Gaunt TR et al. Influence of common genetic variation on blood lipid levels cardio- [13] AhR vas cular risk and coronary events in two British AhR prospective cohort studies J. Eur Heart J [14] 13 : AhR AhR 4 Ye H Li X Wang L et al. Genetic associations with AhR TCDD coronary heart disease: meta-analyses of 12 candidate genetic variants J. Gene : [15] AhR 10 5 Bullen C. Impact of tobacco smoking and smoking rs G>A rs G>A cessation on cardiovascular risk and disease J. Expert Rev Cardiovasc Ther : AhR 10 AhR rs Iwano S Asanuma F Nukaya M et al. CYP1A1-mediated mechanism for atherosclerosis induced by poly- [16] 939 cyclic aromatic hydrocarbons J. Biochem Biophys Res Commun : AhR rs Iwano S Shibahara N Saito T et al. Activation of p53 [ 17] as a causal step for atherosclerosis induced by poly - rs cyclic aromatic hydrocarbons J. FEBS Lett 2006 [18] 554 R554K : [12] SNP 8 Wang XW Li K Guo S et al. The association of AhR AhR554 functional polymorphisms in the aryl hydrocarbon receptor AHR gene with the risk of vitiligo in Han P4501A1 cytochrome P450 1A1 CYP1A1 Chinese populations J. Br J Dermatol : CYP1A1 Msp Chen D Tian T Wang H et al. Association of human C aryl hydrocarbon receptor gene polymorphisms with [19] risk of lung cancer among cigarette smokers in a Chinese population J. Pharmacogenet Genomics 2009 AhR rs AA AG GG 19 1 : Sangrajrang S Sato Y Sakamoto H et al. Genetic polymorphisms of estrogen metabolizing enzyme and AhR AhR breast cancer risk in Thai women J. Int J Cancer AhR :

4 2. rs Humblet O Birnbaum L Rimm E et al. Dioxins and cardiovascular disease mortality J. Environ Health Perspect : Brokken LJ Giwercman YL. Gene-environment interactions in male reproductive health: special reference to the aryl hydrocarbon receptor signaling pathway J. Asian J Androl : Frericks M Burgoon LD Zacharewski TR et al. Promoter analysis of TCDD-inducible genes in a thymic epithelial cell line indicates the potential for cell - specific transcription factor crosstalk in the AhR response J. Toxicol Appl Pharmacol : Kikuchi Y Ohsawa S Mimura J et al. Heterodimers of bhlh -PAS protein fragments derived from AhR AhRR and Arnt prepared by coexpression in Escherichia coli characterization of their DNA binding a ctivity and preparation of a DNA complex J. J Biochem Arsenescu V Arsenescu RI King V et al. Polychlorinated Biphenyl -77 Induces adipocyte differentiation and proinflammatory adipokines and Promotes obesity and atherosclerosis J. Environmental Health Perspectives : Harper PA Wong JM Lam MS et al. Polymorphisms in the human AH receptor J. Chem Biol Interact : Huang S Shui X He Y et al. AhR expression and polymorphisms are associated with risk of coronary arterial disease in Chinese population J. Sci Rep : Safarinejed MR shafiei N Safarinejad S. Polymorphisma in arg hgdrocarbon receptor gene are associated with idiopathic male factor infertility J. Reporod Sci : Wang XL Greco M Sim AS et al. Effect of CYP1A1 MspI polymorphism on cigarette smoking related coronary artery disease and diabetes J. Atherosclerosis : AhR rs is Associated with Coronary Heart Disease MA Jia Dai Xiaojing JIAO Haiyan Abstract: Objective χ Key words:

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