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1 33 9 () V o l. 33 N o Jour. of N o rthw est Sci2T ech U niv. of A gri. and Fo r. (N aṫ Sci. Ed. ) Sep Co Χ Ξ,,, (, ) [ ] 3 60 Co Χ, 3,, 60 Co Χ, Χ, ; 60 Co Χ,,,, ;, 60 Co Χ, 3, 1, 131, 6 [ ] 60 Co Χ; ; ; ; ; ; [] Q ; Q [] A [ ] (2005) ,,,,,,,,,, 60 Co Χ, 85% [1 4 ],,,, [5 ] [6 ] 3 60 Co Χ,, ;, [7 ] Χ,,, 2 ;, ;,, 1,, 60 Co Χ,, Co Χ 60 Co Χ Ξ [ ] [ ] [ ] (1981- ),,,, [ ] (1960- ),,,, E2m ail: J inyinlu@ 163. com
2 58 () 33, 0 ( ), 100, Gy, Gygm in [ 8 ] [ 9 ] 25, cm, 100 Gy 09: 00 13: 00, 300 Gy 500 Gy 12: 00 13: 00Carnoysπs ( % ) 24 h, 1 mo lgl, m in, 24 h, 1 mm,,,, ( ) 8, , O lympus D PS g% = 100% ; g% = 100% ; g% = 100% Co Χ 60 Co Χ Co Χ T able 1 Effects of 60 CoΧ ray irradiation on the m ito sis of tip cells in the w heat seeds of different varieties V arieties 131 Changw u N o Shaanhe N o. 6 1 Zhengyin N o. 1 ggy Irradiation do se : 3 5% ; 3 3 1% N um ber of cells observed N um ber of m ito tic cells g% M ito tic index N o te: 3 is significant at the level, 3 3 is significant at the level. T he sam e fo llow ing tables. 1, 100 Gy %, %, % % ; %, % Gy, ; 300 Gy, %, %, % 500 Gy, %, %, %, t, ; Gy 0. 01, Co Χ1,, (X ) (Y ) : 131 : Y = X X (r 2 = ) ; 6 : Y = X X +
3 9 : 60 Co Χ (r 2 = ) ; 1 : Y = X X (r 2 = ) Co Χ 60 Co Χ g (g ),,,, (g 21) ;, (g 22) Χ, 2,,, (g 23, 4) ; 1 2,, 1 (g 25) ; 1 1, (g 26) g 60 CoΧ 1. ; 2. ; 3. ; 4. ; 5. ; 6. P lateg Ch romo som al aberration on roo t tip cells in w heat seeds after irradiated by 60 Co Χ ray 1. T elophase of regular m ito sis; 2. L atter telophase of regular m ito sis; 3. B ridge ; 4. M ulti2 bridge; 5. M ulti2 bridge and F ragm ents ; 6. M icronucleus 2, 3 2, Χ (), ;,
4 60 () 33 V arieties 131 Changw u N o Shaanhe N o. 6 1 Zhengyin N o Co Χ T able 2 Statistics ch romo som al aberration and nuclear aberration of roo t tip cells in the w heat seeds of ggy Irradiation do se different varieties irradiated by 60 CoΧ ray N um ber of cells observed Sticky Types of chromo som al aberration F ragm ents B ridge M ulti2bridge N um ber of m icro2 nucleus To tal , (A ) (X ) : 131 : A = X X (r 2 = ) ; 6 : A = X X (r 2 = ) ; 1 : A = X X (r 2 = ) : (B ) (X ) 131 : B = X X (r 2 = ) ; 6 : B = X X (r 2 = ) ; 1 : B = 0. 01X X (r 2 = ) 3 60 Co Χ T able 3 Effects of ch romo som al aberration and nuclear aberration on roo t tip cells in the w heat seeds of V arieties 131 Changw u N o Shaanhe N o. 6 1 Zhengyin N o. 1 3 ggy Irradiation do se different varieties irradiated by 60 CoΧ ray, N um ber of cells observed g% Chromo som al aberration rate g% Rate of m icronucleus , 60 Co Χ, 100 Gy ; Gy,
5 9 : 60 Co Χ h, 60 Co Χ,,,,,, 60 Co Χ : 131 6, ; 1, 3,,,,,, (M 1 ) [10 ], 60 Co Χ [11 ],, [12 ], 60 Co Χ, 1,, ;,,, [11 ], 60 Co Χ3,, 3, : 1 > 131 > 6, 3, 1 Χ, Cer2 m eno [13 ],,, 1 60 Co Χ,,,, [14 ] 24,,, [15 ], 1,,, :, [ 1 ]. [M ]. :, [ ] [ 2 ] M aluszynsk im K, N ich terlein L, Bhatia C R, et al. O fficially released m utant varieties2the FAO gia EA database[j ]. M utation B reeding Review, 2000, 12: [ 3 ]. [J ]., 1998, 20 (1) : [ 4 ] U kai Y. V arietal difference in gamm a2ray induced aberration in soybean[j ]. Jap J Genetics, 1980, 55: [ 5 ],,,. [J ]., 2002, 16 (4) : [ 6 ],,. Χ[J ]., 1996, 10 (3) : [ 7 ],,,. ( X ). g. [J ]., 2000, 14 (4) : [ 8 ],,,. 60 Co Χ [J ]., 2003, 30 (1) : [ 9 ]. [M ]. :, 1985: 108 [ 10 ],. M 1 [J ]., 1994, (1) : [ 11 ],,,. 60 Co Χ [J ]., 1996, 16 (2) : [ 12 ]. [M ]. :,
6 62 () 33 [ 13 ] Cerm eno M C, L acadena J R. C2banding analysis of gamm a2radiation2induced ch romo som al interchanges in rye: T he ro le of C2heterom tin in chromo som e repatterning[j ]. Chromo som a, 1985, 91: [ 14 ],,. [J ]., 1995, 16 (2) : [ 15 ],. 60 Co Χ [J ]., 2001, 15 (1) : Effects of 60 Co Χ ray irradiation after m ito sis on roo t tip cells in w heat seeds L U L i,l U J in-y in,l IU Jun, GAO Jun-feng (Colleg e of L if e S ciences, N orthw est A & F U niversity, Y ang ling, S haanx i , Ch ina) Abstract: Studies of m ito sis effects on roo t tip cell in w heat seeds of differen t varieties irradiated by 3 k inds of do sages of 60 Co Χ ray indicated that the irradiation of low er do sage by 60 Co Χ ray had tw o effects on the m ito sis of tip cells, bu t the m ito tic index of roo t tip cells decreased at differen t degrees w ith do sages increased. Fu rthermo re, the irradiation of 60 Co Χ ray cou ld induce m any types of ch romo som al aberration and nuclear aberration in roo t tip cells, the rate of ch romo som al aberration and m icronucleu s aberration of roo t tip cells increased at differen t degrees w ith do sages increased. T he experim en tal resu lt also indicated that Zhengyin N o. 1 w as mo re sen sitive than o thers, then Changw u N o. 131 and Shaanhe N o. 6 w as least sen sitive. Key words: 60 Co Χ ray; w heat seeds; irradiation induced b reeding; m ito tic index; rate of ch romo som al aberration; rate of m icronucleu s aberration; irradiation do se ( 56 ) Abstract ID: (2005) EA M icro scop ic ob servation on som atic clones from imm atu re w heat em b ryo s and on their changes du ring subcu ltu res L IANG Hong 1, CHEN Yao-feng 2,W ANG Rui-hui 1, REN Hui- li 2,L I Chun- lian 2, HAN D e- jun 2 (1 Colleg e of A g ronomy, H ebei A g ricu ltu ral U niversity, B aod ing, H ebei , Ch ina; 2 Colleg e of A g ronomy, N orthw est A & F U niversity, Y ang ling, S haanx i , Ch ina) Abstract: W ith scann ing elect ron m icro scop e (SEC ), changes of som aclones induced from imm a tu re w heat em b ryo du ring subcu ltu res, and differences betw een em b ryogen ic (EC) and non2em b ryogen ic clones (N EC) w ere investigated. T he resu lts show that m icro scop ically structu ral differences betw een N EC and EC are ascribed to differen t structu ral fo rm s of em b ryogen ic cells betw een w heat cu ltivars X iaoyan 22 and Q ian jinzao. Em b ryogen ic clones w ith h igh induction frequency in X iaoyan 22 po ssess good cell o r2 gan izaion s, w h ile EC w ith low er induction frequency in Q ian jinzao po ssess relatively poo r cell o rgan iza2 tion. Fu rthermo re, the em b ryogen ic clones show a tendency of diso rder w ith variation betw een X iaoyan 22 and Q ian jinzao. X iaoyan 22 can su rvive the con tinuou s subcu ltu res, and its con tact o rgan ization of cells can be retained w ith h igh o rder fo r a long tim e, w h ile fo r Q ian jinzao, w ho se em b ryogen ic clones w ere p roduced w ith low frequency, o rgan ization of its cells can on ly be retained in lim ited generation s of subcu ltu res and tend to b reak up. Key words: w heat (T riticum clones; em b ryogen ic cells aestivum L. ) imm atu re em b ryo; em b ryogen ic clones; non2em b ryogen ic
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