Ch inese Traditional and Herbal D rugs

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1 1215,, DNA H epg2 DNA, (H PCE) H epg2 DNA, H PCE DNA,, 5 m dc,,, DNA 5 m dc,, H epg2 DNA DNA,,,,, DNA, DNA,, CpG, G1,, DNA, : [ 1 ], 1 [J ]1, 2005 (2) : [ 2 ] Slow S, L ever M, L ee M B, et al. Betaine analogues alter homocysteine m etabo lism in rats [J ]1 Int J B iochem Cell B iol, 2004, 36 (5) : [ 3 ] U eland P M, Ho lm P I, H ustad S1 Betaine: a key modulato r of one2carbon m etabo lism and homocysteine status [J ]1 C lin Chem L abm ed, 2005, 43 (10) : [ 4 ] Schw ahn B C, W endel U, L ussier2cacan S, et al. Effects of betaine in a m urine model of m ild cystathionine2beta2synthase deficiency [J ]1 M etabolism, 2004, 53 (5) : [ 5 ] 1 [J ]1, 2007, 19 (2) : [ 6 ],,, 1 H epg2 [J ]1, 2008, 39 (6) : [ 7 ] F raga M F, U rio l E, Bo rja D L, et al. H igh2perfo rm ance cap illary electropho retic m ethod fo r the quantification of 52 m ethyl 22deoxycytidine in genom ic DNA : app lication to p lant, anim aland hum an cancer tissues [J ]1 E lectrop horesis, 2002, 23: ,,,,, Ξ (, ) : H PL CgDAD,, H PL CgDAD, D ikm a D iamonsil C 18 (250 mm 416mm, 5 Λm ); 30 ; % ( ), 1 ml gm in; 320 nm ( ) 430 nm ( ); 20 ΛL, ΛggmL 1 40 ΛggmL, (R SD ) 311%, 99148% 10212% ( m ggml ) 7217%, 6717% 5616% ; ( ΛggmL ) (K a) h - 1, (P > 0105) H PL CgDAD,,,,, : ; H PL CgDAD; : R : A : (2008) : : (C ) : (1972 ),,,, T el: (0311) E2m ail: w angcy730301@ 163. com 3 T el: (0311) E2m ail: zhanglantong@ 263. net

2 1216 Ch inese Traditional and Herbal D rugs , 3, 5, O 2Β2D 2 ( ),, [1 2 ] [3 5 ] [6, 7 ],, 2 [8 ],,,, 1 A gilen t T echno logies 1200 series ( 2,,, ) ; SCQ 200 ( ) ; SZ 93 ( ) ; HL 1S ( ), 9813% ;, ; ; ;,, (250 20) g, : D ikm a D iamon sil C 18 (250 mm 4. 6 mm, 5 Λm ) ; 30 ; % ( ), 1 ml g m in; 320 nm ( ) ; : 20 ΛL 212 ( ) 430 nm :,,, m ggml, K reb s2r inger (ph 7. 4, K2R ) : N ac l 7. 8 g KC l g CaC l g N ahco g N ah 2PO g M gc l g 114 g, ml, :, K2R 20 ΛggmL, :, :, 011 mo lgl HC l m ggml, 213 :, 50 ml, 011 mo lgl HC l,, 011 mo lgl HC l,, rgm in 10 m in 0145 Λm, 20 ΛL, 320 nm, 214 :, rgm in 10 m in 0145 Λm, 20 ΛL, 320 nm 430 nm, :,,, : ml, 10 ml,, 20 ΛL,,, 320 nm 430 nm,,, : ΛggmL, ΛggmL 210 ml, 213, ;, 214, 5, 1 d, ; 5, 3 d, : m ggml, 37, h 210 ml, 213, ; m ggml, ΛggmL, 3 7, 0 015

3 stilbeneglyco side 22pheno lsulfonph thelein nm (A) 430 nm (B) 320 nm (C) 430 nm (D ) 320 nm (E) 430 nm (F) 320 nm (G) HPLCgDAD F ig. 1 HPLCgDAD Chromatogram s of m ixed solution of stilbene glycoside and phenolsulfonphthale in in methanol at 320 nm (A) and 430 nm (B), blank in testinal c irculation solution at 320 nm (C) and 430 nm (D ), sample of in testinal absorption at 320 nm (E) and 430 nm (F), and sam ple of stomachal absorption at 320 nm (G) h, 214, 216 [8, 9 ] : 12 h ( ), (250 20) g SD, 6 ml g kg ip 25%,,,,,,,, 210 ml,, 37 1 h, 213,,, ( - ) : m ggml, [10, 11 ] : 12 h ( ), (250 20) g SD, 6 ml g kg ip 25%,,, 013 cm,, 37,, 37, 5 ml gm in 10 m in, 215 ml g m in, 110 ml, 110 ml, 0, 15 m in, 2 h, 214, F ick, ( lnx ) ( t), ( lnx = lnx 0- K at), K a, (t1g2), t1g2= gK a; (P ), P = (C 0 V 0- C t V t) g (C 0 V 0 t) 100% (C 0: 0 ; V 0: 0 ; C t: t ; V t: t ; t: ) : ΛggmL, : A = C , r= , 1 40 ΛggmL ; 1,, ( ΛggmL ), 215 h R SD 011%, 312 : A = C , r= , ΛggmL 1,,

4 1218 Ch inese Traditional and Herbal D rugs ( m ggml ), 6 h R SD 015%, ; ( m ggml ), 215 h R SD %,,,, : ( m ggml ), 37, 15 m in 110 ml,, [D t, D t= (1- C tgc 0) 100%, C 0: 0 ; C t: t ] : = + D t 1 (n= 5) Table 1 Prec ision and accuracy of stilbene glycoside and phenolsulfonphthale in in blank stomachal perfusate and blank in testinal c irculation solution (n= 5) g(λg ml - 1 ) (x s) g% RSDg% RSDg% : 1 h, 2,,,, (P > 0105), (P < 0105),,,, 2 Table 2 Absorption of stilbene glycoside in stomach in rats g(λg ml - 1 ) g% RSD g% : 2 h, K a t1g2 P 3, r 019,, K a,, K a (P > 0105) ΛggmL,, 5%,, 3 (x s, n= 5) Table 3 Absorption parameters of stibene glycoside at var ious concen tration in in testine in rats (x s, n= 5) g(λg ml - 1 ) K agh - 1 t1g2gh P g% r ,,,,,,,,,, 37,,,,,,,,,,,,, ig,,, [12 ] g,,

5 1219,,,,,,,,,,,,, : [ 1 ] Ryu G, Ju J H, Park Y J, et a l. T he radical scavenging effects of stibene gluco sides from P oy gonum m ultif lorum [J ]1 A rch P harm R es, 2002, 25 (5) : [ 2 ] L v L S, Gu X H, T ang J, et a l. A ntioxidant activity of stilbene glyco side from P oy gonum m ultif lorum Thunb. in v ivo [J ]1 F ood Chem, 2007, 104 (4) : [ 3 ],,, 1 D 2 [J ]1, 2005, 16 (1) : [ 4 ],,, 1 D 2 [J ]1, 2005, 19 (1) : [ 5 ],,, 1 A PP [J ]1, 2006, 15 (3) : [ 6 ],, 1 [J ]1, 2007, 32 (4) : [ 7 ] Yang P Y, A lmofti M R, L u L, et al. Reduction of athe2 ro sclero sis in cho lestero l2fed rabbits and decrease of exp re2 ssions of intracellular adhesion mo lecule21 and vascular endo thelial grow th facto r in foam cells by a w ater2so lution fraction of P oly gonum m ultif lorum [J ]1 J P harm acol S ci, 2005, 99 (3) : [ 8 ] Passamonti S, V rhovsek U, V anzo A, et al. T he stom ach as a site fo r anthocyanins abso rp tion from food [J ]1 F EB S L ett, 2003, 544 (123) : [ 9 ] Zhao Z, Egash ira Y, Sanada H, et al. Ferulic acid is quick ly abso rbed from rat stom ach as the free fo rm and then conjugated m ainly in liver [J ]1 J N utr, 2004, 134 ( 11 ) : [ 10 ],,, 1 [J ]1, 2006, 15 (18) : [ 11 ], 1 [J ]1, 2006, 31 (12) : [ 12 ],,, 1 [J ]1, 2005, 36 (3) : M B49 1, 2, 3, 1, 1, 1Ξ (11, ; 21, ; 31, ) : M B 49 M T T M B 49 ; Hoech st ; A nnexin V 2F ITC2P I ; caspase23 caspase28 caspase29 ; W estern blo tting Bcl22 Bax M B 49, 2 2,,,,M B 49 Caspase23 Caspase29 ; Bcl22, Bax, Caspase28 M B 49 FasgFasL 2 Bax Bcl22, M B 49 : ; ; ; Caspases : R : A : (2008) R abd osia rubescens (H em sl. ) H ara, [1 5 ], [3 ] ; M B 49, M B 49, Ξ : : (1967 ),,,,, T el: (0898) E2m ail: chexianp ing@ 126. com