CHEM ICAL JOU RNAL O F CH IN ESE UN IV ERS IT IES M osbach [1, 2 ] ml :

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1 V ol. 25 N o CHEM ICAL JOU RNAL O F CH IN ESE UN IV ERS IT IES N - -L -,,,, (,, ), 22 (22V P ), (D EGDA ), N 2 2L 2 (N 2Cbz2L 2T rp),., 6. 3 Λm, ,.,. ; ; O 631 A (2004) ,. M osbach [1, 2 ]. [3 5 ].,,,,, [6 ] ;,, [7 ].,,. 1994, Hosoya [8 ] [9 ],., N 2Cbz2L 2T rp,, (St),, ; 22 (22V P), Sigm a, ; (D EGDA ),, ;. (A etonitrile),, ; (DBP),, ; (SD S),, ; (A IBN ),, ; (PVA ), ;. 2L 2 (N 2Cbz2L 2T rp), 2D, L 2 (N 2Cbz2D, L 2T rp) 2L 2 (N 2Cbz2L 2T yr) Sigm a (KSP), [10 ]. 610% ( ). 30 ml 5 m in,, 18 h.,,. 90 ml : : ( : ). : (1965 ),,,,. E2m ail: tyguo@nankai. edu. cn

2 N o. 4 : N 2 2L m in,.., N 2, 50, 180 rgm in. 24 h,, TH F 3 24 h PVA, SD S., H itach i X2650 ( ), ; AU TO SORB21 (Q uantach rom e Corporation, Germ any) ; R FS100gS FT 2R am an ( B ruker ), : N d YH G, Κ= nm ; N ET ZSCH T G209, :, , Kgm in; (Scientific System Inc, SS I), L aba lliance,, 50 mm 4. 6 mm ( i. d. ),, M Pa,,, 10 ΛL, 500 ng, [ 11 ]. kr = (tr- t0) gt0; R S= 2 (tl + td ) g(w L + W D ), N R = (trgw 1g2), tl, td L 2, D 2, t0, W L, W D, W 1g2 L 2, D [12 ], N 2Cbz2L 2T rp 1 22,,,. : 1.,.,, BET, ml g,.,.. F ig. 1 SEM images of M IP m icrospheres prepared by two- step swell ing and suspen sion polymer iza tion method, ( 2). 2, 6 7 Λm 90%, 6. 3 Λm, d p= (d p= dw gd n, dw, d n ),, ,, ;

3 764 V ol. 25 F ig. 2 The distr ibution of the diameter of M IP m icrospheres F ig. 3 Thermograv ity curves of un iform - sized m olecularly im pr in ted m icrospheres used 2- VP a s the functiona l monomer 300, ; 400 ; , %,. 4 (A ) (B ). 4 (A ), cm - 1 1, ; cm - 1 ; cm V P. F ig. 4 Raman spectra of un iform -sized molecularly impr in tied polymer m icrospheres before remov ing templa te molecule (A) and af ter be ing eluted by methanol (B) cm - 1, N 2Cbz2L 2T rp,, 1,, cm cm - 1 ; cm - 1, cm V P D EGDA. 4 (B ), N 2Cbz2L 2 T rp ( cm - 1 ),, ng N 2Cbz2D, L 2T rp 50 mm 416 mm (i. d. ) N 2Cbz2L 2T rp F ig. 5 Ch ira l separa tion on the N -Cbz-L -Trp impr in ted. 5, N 2Cbz2D 2T rp, N 2Cbz2L 2T rp, un iform - sized m icrospheres Colum n: 50 mm 4. 6 mm ( i. d. ) ; samp le amount: 500 N 2Cbz2L 2T rp, ( : kd = 0. 14, ng in 10 ΛL; mobile phase: acetonitrile; flow 2rate: 0. 5 ml gm in; 280 nm; 30.

4 N o. 4 : N 2 2L kl = 5. 10, R S = 1. 22, N D = 70, N L = 11).,.,,, N 2Cbz2D, L 2T rp, D 2,,, ; L 2,,,, D 2, L 2,. N 2Cbz2L 2T rp N 2Cbz2L 2T rp [4 ].. N 2Cbz2L 2T rp N 2Cbz2L 2T yr N 2Cbz2L 2T rp. [ 1 ] A ndersson L. İ, M osbach K.. J. Chrom atogr[j ], 1990, 516: [ 2 ] Yu C., M osbach K.. J. Chrom atogṙ A [J ], 2000, 888: [ 3 ] Sellergren B., Shea K. J.. J. Chrom atogṙ A [J ], 1993, 654: [ 4 ] WAN G J in2fang ( ), ZHOU L iang2m o ( ), M EN G Zi2Hui( ) et al.. A cta Chim ica Sinica( ) [J ], 1999, 57: [ 5 ] CHEN G Guo2Xiang ( ), ZHAN G L i2yong ( ), FU Cong ( ). Chinese Journal of Chrom atography ( ) [J ], 2002, 3: [ 6 ] B r ggem ann O., Haup t K., Yilm az L. E. et al.. J. Chrom atogṙ A [J ], 2000, 889: [ 7 ] Snyder L. R., Kirkland J. J.. Introduction to M odern L iquid Chrom atography (2nd ed. ) [M ], N ew York: W iley, 1979 [ 8 ] Hosoya K., Yoshizako K., Tanaka N. et al.. Chem. L etṫ [J ], 1994, [ 9 ] U gelestad J.. M akromol. Chem. [J ], 1978, 179: [ 10 ] S ε m igolv., S ε vec F., Hosoya K. et al.. A ngew. M akro. Chem ie. [J ], 1992, 195: [ 11 ] Haginaka J., Kagaw a C.. J. Chrom atogṙ A [J ], 2002, 948: [ 12 ] F itch R. M., P renosilm. P., Sp rick K. J.. J. Poly. Sci., Part C [J ], 1969, 27: Preparation of Un iform - sized M olecularly Impr in ted Polymer M icrospheres Using N -Cbz-L -Trp as Template GUO T ian2ying 3, ZHAN G L i2ying, HAO Guang2J ie, SON G M ou2d ao, ZHAN G Bang2H ua (S tate K ey L aboratory of F unctional P olym er M aterials f or A d sorp tion and S ep aration, Institute of P olym er Chem istry, N ankai U niversity, T ianj in , Ch ina) Abstract U niform 2sized molecularly imp rinted polym er m icrospheres w ere p repared by using N 2 benzyloxycarbonyl2l 2tryp tophane (N 2Cbz2L 2T rp) as the temp late v ia a m ulti2step sw elling and suspension polym erization m ethod in w h ich 22vinylpyridine (22V P) w as used as the functionalmonom er and diethylene glyco l diacrylate (D EGDA ) w as used as the cro ss2linkeṙ T he surfaces of nonpo rous m icropheres w ere rough. T he average diam eter of polym er m icropheresw as 6. 3 Λm, and its distribution coeffiene w as It w as found that the baseline ch iral separation of the temp late enantiom er w as ach ieved on a shorter colum n. M oreover, the removing of the temp late molecule and the therm al stability of M IP m icropheres w ere also discussed. Keywords M olecularly imp rinted polym er; U niform 2sized dispersion; Am ino acid derivatives (Ed. : W, Z)

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