mm % H 2O 2 10 m in SABC (Strep t A vidin2b io tin Comp lex)
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1 34 11 () V o l. 34 N o Jour. of N o rthw est Sci2T ech U niv. of A gri. and Fo r. (N aṫ Sci. Ed. ) N ov α 1, 2, 1 (1, ; 2, ) [](testo sterone, T ) (B atrachup erus tibetanus),, 4 T ; 5 T ; 67, T ; 8, T ; 910, T ; 11,, 411 T, T , T,, T [] ; ; ; []Q []A [ ] (2006) ,,,,,, 1, (S a lam and ra sa lam and ra ) [1 ] ( A m by stom a tig rinum ) [2 ] (P leu rod eles w a ltl) [3 ] (T ritu rus m a rm ora tus) [4 ],,, ;, (B a trachup erus tibetanus),, 2, , 6 7, 7 8 [5 ], (T esto sterone, T ), T,, , 2030 g, mm , , Bo in s ( ) 1012 h,,,, 7 Λm 1. 3, mo lgl (ph 6. 0) 23 3% H 2O 2 10 m in SABC (Strep t A vidin2b io tin Comp lex) α [ ] [ ] (2001 SM 26) ; (04YKZ009) [ ] (1972 ),,,,, [ ] (1956 ),,,, China Academic Journal Electronic Publishing House. All rights reserved.
2 16 () 34, PBS, 2 4, T (SPGg ) ( 1) 5, SPGg (SPC), T ( 2)T 4 5 (L C) (SC) ( 2, 3)6 7, (SPC) (SPD ) T ( 4, 5)8, ( Imm atu re R egion) (M atu re R egion), ( 6)910, SPG T, L C, SC (SPZ) ( 7, 8, 9) 11, SPG SPZ, T ( 10, 11), L C 411, (SPGg ), T ( 12, 8) ; SPGg, ( 13) T ( 14) 1,, T SPGg, SPC SPD T, L C SC, T 1 T able 1L ocalization of testo sterone imm uno reactivity in the testis of B atrachup erus tibetanus during the annual cycle M onth SPGg SPGg SPC SPD SPZ L C SC 4 Cp (+ + ) N p (+ ) Cp (+ ) 9 Cp (+ + ) Cp (+ + ) N p (+ ) Cp (+ ) + : Cp. ; N p. ; + +. ; +. ;. N o te: Cp. Cytop lasm ; N p. N ucleop lasm ; + +. m edium imm unopo sitive reaction; +. w eak imm unopo sitive reaction;. negative reaction. 13(T ) 1. 4, T (316) ; 2. 4, (316) ; 3. 4 (316) P late 1 3R esults fo r imm unoh istochem istry of testo sterone (T ) in the testis of the stream salam ander B atrachup erus tibetanus 1. T he testis in A p r, testo sterone w as p resented in the cytop lasm of secondary sperm atogonia (316) ; 2. In A p r, T po sitive reactivity w as detected in the nuclear of p rim ary sperm atogoni, the reactivity w as stronger w hen the cells divided (316) ; 3. L eydig cells and serto li cells reacted po sitively in A p r (316) China Academic Journal Electronic Publishing House. All rights reserved.
3 11, : (T ) 4. 6, (316) ; 5. 7, (316) ; 6. 8, (316) ; 7. 9, (316) ; 8. 9, (158) ; 9. 10,, ( 316) ; ,, (316) ; , SPZ, T (316) ; 12. 4, (316) ; 13. (316) ; 14. (316) P late 4 14R esult fo r imm unoh istochem istry of testo sterone (T ) in the testis of the stream salam ander B atrachup erus tibetanus 4. T po sitive reactivity w as detected in the nuclear of sperm atocyte during Jun (316) ; 5. In Jul, T po sitive reactivity w as detected in the nuclear of sperm atid (316) ; 6. In A ug, there w ere T sligh t po sitive reaction do ts scattered in imm ature region and m ature region (316) ; 7. In Sep, T w as p resented in L eydig cells and Serto li cells and sperm atozoa in m ature region (316) ; 8. In Sep, T w as detected in the nuclear of SPG I and cytop lasm of SPG of the far end in imm ature region (158) ; 9. In O ct, T w ere detected in cytop lasm of SPG of the far end in imm ature region, L eydig cells, serto li cells and sperm atozoa in m ature region (316) ; 10. In N ov, w eak po sitive reactivity w as in the cytop lasm of SPG in imm ature region; L C w ere negative reaction (316) ; 12. In A p r, T po sitive reactivity w as detected in the nuclear of p rim ary sperm atogoni, the reactivity w as stronger w hen the cells w ere dividing (316) ; 11. In N ov, there w as sligh t po sitive reaction in SPZ in m ature region (316) ; 13. A blastom ere of SPGg w as po sitive reactivity (316) ; 14. Contro l slide w as negative reaction (316) China Academic Journal Electronic Publishing House. All rights reserved.
4 18 () ,, Garn ier [3 ] (P leu rod eles w a ltl), 3, ;, (R ana escu len ta), [6 ], [1, 2, 7 ],, [2 ] L azard [7 ] (A m by stom a ax olotl),,,, [8 ] Specker [1 ],, Specker [1 ],,, SPG I T, SPG I,, SPGg, SPGg, SPC SPD, T, T,,,,, 3. 2 [9 ] [1 ] [2 ] [3 ] [10 ], T (T ), ,,L C T, T, T F rail [4 ], T [1, 3 ],,, [4, 11 ], [12 ],, 520 d, (FSH ) [13 ],,,,,,, SC T, SC,, T SC, T, SC, [14 ] T SC L C, 2, 3. 3,, DNA, DNA, T, SPGg SPC, SPG, SPC SPD : T, T,, 45 IC, China Academic Journal Electronic Publishing House. All rights reserved.
5 11, : 19,, SPC ;, T, 12 2, 911, T SPZ, T SPZ, 8, T, T, :,,,,,,,,,,,,,,,,, [ ] [ 1 ]Specker J L,M oo re F L. A nnual cycle of p lasm a androgens and testicular compo sition in the rough2sk inned new t, T aricha g ranu losa [J ]. Gen Comp Endocrino l, 1980, 42 (3) : [ 2 ]N o rris D O, N o rm an M F, Pancak M K, et al. Seasonal variations in sperm atogenesis, testicular w eigh ts, vasa deferentia, and androgen levels in neo tenic m ale tiger salam anders, A m by stom a tig rinum [J ]. Gen Comp Endocrino l, 1985, 60 (1) : [ 3 ] Garnier D H. A ndrogen and estrogen levels in the p lasm a of P leurodeles W altl, M ichah, during the annual cycle [ J ]. Gen Comp Endocrino l, 1985, 58 (3) : [ 4 ]F rail B, Paniagua R, RodriguezM C, et al. A nnual changes in the num ber, testo sterone content and ultrastructure of glandular tissue cells of the tesis in the m arbled new t T ritu rus m a rm ora tus[j ]. J A nat, 1989, 167: [ 5 ],. [J ]., 2004, 25 (6) : [ 6 ]D πistriam, D elrio G, Bo tte V, et al. Radio imm unoassay of testo sterone, 172Βoestradio l and oestrone in the m ale and fem ale p lasm a of R ana esculenta during sexual cycle[j ]. Stero ids lip ids Res, 1974, 5: [ 7 ]L azard L. Stero idogenesis in axo lo tl testis. H istochem istry of two m ajo r enzym es related to cell type, sperm atogenesis, and substrate[j ]. Gen Comp Endocrino l, 1979, 39 (3) : [ 8 ]Callard I P, Callard G V, L ance V, et al. T esticular regulation in nonm amm alian vertebrates[j ]. B io l Rep rod, 1978, 18 (1) : [ 9 ]Itoh M, Inoue M, Ish ii S. A nnual cycle of p ituitary and p lasm a gonado trop ins and p lasm a sex stero ids in a w ild population of the toad, B uf o jap on icus[j ]. Gen Comp Endocrino l, 1990, 78 (2) : [10 ]Fasano S, M inucci S, D i M atteo L, et al. Intratesticular feedback m echanism s in the regulation of stero id p rofiles in the frog, R ana escu len ta[j ]. Gen Comp Endocrino l, 1989, 75 (3) : [ 11 ]A sah ina K, Suzuk i K, A ida K, et al. Relationsh ip betw een the structures and stero idogenic function of the testes of the U rohaze 2goby (G lossogobius olivaceus) [J ]. Gen Comp Endocrino l, 1985, 57 (2) : [ 12 ]. serto li [J ]., 1991, 37 (2) : [13 ] Callard G V, canick J A, Pudney J. E strogen synthesis in leydig cells: structrural2functional co rrelations in necturus testis [J ]. B io l Rep rod, 1980, 23 (2) : [ 14 ],. [J ]., 2001, 32 (4) : L ocalization of testo sterone in the testis of the stream salam ander B a trachup erus tibetanus du ring the annual rep roductive cycle L IYa lin 1, 2, ZHANG Y uhui 1 (1 Colleg e of L if e S cience, S haanx i N orm al U niversity, X iπan, S haanx i , Ch ina; 2 Chem istry D ep artm ent,w einan T eacher College,W einan, S haanx i , China) Abstract: L oca t ion of testo sterone ( T ) in test is of B a trachup erus tibetanus w a s exam ined by China Academic Journal Electronic Publishing House. All rights reserved.
6 20 () 34 imm unoh istochem istry. T he resu lts w ere as fo llow s: testo sterone w as p resen ted in the cytop lasm sperm atogon ia (SPG) in A p ril. It w as detected in the nuclear of SPG in M ay w hen SPG w ou ld becom e p rim ary sperm atocyte ( ISPC ). D u ring Jun2Ju l, T po sitive reactivity w as also detected in the nuclear of sperm atocyte (SPC) and sperm atid (SPD ). In A ug, there w ere T sligh t po sitive reaction do ts scattered in the imm atu re region and m atu re region. In Sep 2O ct, T w as p resen ted in the cytop lasm of SPG in imm atu re region and sperm atozoa (SPZ) in m atu re region. T po sitive reactivity w eakened in the cytop lasm of SPG and SPZ in N ov. D u ring the annual rep roductive cycle, T po sitive location alw ays p resen ted in the certain part in the testis. T w as also detected in L eydig cells (L C) and Serto li cells (SC) of the testis du ring A p r2 M ay and Sep 2O cṫ T he resu lt show s that testo sterone disperses periodically and regu larly in testis of Batrachuperu s tibetanu s and it p lays an regu lative ro le in p ro liferation of germ cell and sperm atogenesis. Key words: B a trachup erus tibetanus; testo sterone; sperm atogenesis; annual rep roductive cycle of (14 ) Abstract ID: (2006) CA Study on po lym o rph ism s of part sequence of in tron 5 and exon 6 of bovine U CP 3 gene L I Qiu ling 1, XU Shangzhong 2, ZAN L in sen 1, GAO Xue 2, ZHANG Zhengfeng 1, REN Hongyan 2, CHEN J inbao 2 (1 Colleg e of A nim al S cience and T echnology, N orthw est A & F U niversity, Y ang ling, S haanx i , Ch ina; 2 Institute of A nim al S cience, Chinese A cademy of A g riculture S cience,b eijing , China) Abstract: T ake Ch inese Simm en tal, A ngu s and H eifo rd as experim en t m aterials, a pair of p rim ers fo r the part of in tron 5 and exon 6 of uncoup ling p ro tein 3 gene w ere designed. T he po lymo rph ism s w ere analyzed by PCR 2SSCP and DNA sequencing. T he resu lts show ed that allele B w as the p redom inance allele in these th ree population s, geno type AA w as the p redom inance geno type in Ch inese Simm en tal and A ngu s and it w as AB in H eifo rd. T here w as no sign ifican t difference (P > 0. 05) in U CP 3 locu s of bo th Ch inese Simm en tal and H eifo rd, w h ile sign ifican t differences ex isted in A ngu s. A nalysis of po lymo rph ism s index show ed the po lymo rph ism of the locu s w as moderate in Ch inese Simm en tal and H eifo rd and h igh in A ngu s. Key words: bovine; U CP 3; SN P s; geno type; allele China Academic Journal Electronic Publishing House. All rights reserved.
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