,,,,,, (C 10 0 C 14 0) (C 4 0 C 8 0) [1 ],, , , 22, 23, : 00 14: 30 2, 20 ml, 4, 2 d, 4 2, 1-20, ; 1,

Transcript

1 33 3 ( ) V o l. 33 N o Jour. of N o rthw est Sci2T ech U niv. of A gri. and Fo r. (N aṫ Sci. Ed. ) M arch 2005 Ξ,,,,,, (, ) [ ], 5 87,, (C4 0) (C 6 0) (C 8 0) (C 10 0) (P > 0. 05); (C 12 0) ( C 14 0) (P < 0. 05); (C 18 2) (P > 0. 05), (C 16 0) (C 18 0) (C 18 1) (P < 0. 05) (P > 0. 05), (P < 0. 05) [ ] ; ; ; [ ] S879. 1; T S [ ] A [ ] (2005) (C 10 0 C 14 0) (C 4 0 C 8 0) [1 ],, [2 ], [3 5 ],,, [1, 6 8 ], ; [4 ],,,,,,,,, , , 22, 23, : 00 14: 30 2, 20 ml, 4, 2 d, 4, 2, 1-20, ; 1,, D eeth [9 ], : - 20,,, 12 ml 50 ml, 18 ml g ( 3 2), 1 m in, rgm in 10 m in ( ) 1 50 ml, 8 ml Ξ [ ] [ ] (1965- ),,,,, E2m ail: luo jun6566@ sina. com

2 3 : 25 5% ( 1 10 ), h, 12 ml 6%,, rgm in 10 m in ( ), 0. 5 g 50 ml,, m in,, (F ID ) GC27A, A T 2FFA P (30 m mm 1. 0 Λm ), 80 2 m in, 32 gm in m in 250, 5 ml gm in, 60 ml gm in, 500 ml gm in, 40 ml gm in, 0. 2 ΛL N 2000,,, F ig. 1 Gas ch rom atogram of goat m ilk fatty acids analysis m in, M ilko2scan 134A gb 1. 5 SPSS, F (L SD ) ,, C 4 0, C 6 0, C 8 0 C 10 0 (P > 0. 05) 1 C %, (P < 0. 01) C ( P < 0. 05), (P > 0. 05), C 18 2 (P > 0. 05), C 18 0 C , (P > 0. 05),, 1 3, 4, 5, 2 4, 5 (P < 0. 05), 1 4, 5 (P < 0. 01) 1 2, 4, 5 (P < 0. 05), 3

3 26 ( ) 33 (P > 0. 05) 1 T able 1 Fatty acid compo sition of fresh m ilk from X inong Saanen dairy goats in different parities ggkg Fatty acid Parities 1 (n= 21) 2 (n= 22) 3 (n= 23) 4 (n= 9) 5 (n= 12) C C C C C a ab ab b ab C a b ab ab ab C b a b b b C a b ab ab ab C c bc a ab ab C : 1 (P > 0. 05), (P < 0. 05) N o te: The sam e letters in the sam e row indicated the non2significant differences ( P > 0. 05) ; w ith the different letters indicated the significant differences (P < 0. 05). It is sam e in the fo llow ing table. 2 T able 2 M ilk compo sition of fresh m ilk from X inong Saanen dairy goats in different parities ggkg M ilk compo sition Parities 1 (n= 20) 2 (n= 22) 3 (n= 23) 4 (n= 9) 5 (n= 12) Fat a ab bc c c P ro tein a bc ab c bc L acto se To tal so lids a ab bc c bc So lid2non2fat a bc ab c bc ,,,,, C 6 0, C 8 0, C g, g, g,, 3, C 4 0 ; C 6 0, C 8 0 C 10 0, [10 14 ],, 3. 2, C 4 0, C 6 0, C 8 0 C 10 0 ; C , 4,, ; C 14 0, C 18 0,, Β2,, COA, [15 ],, 3. 3 [2 ]

4 3 : 27,, 1, 2, 3, 4, 5 ; 2, 3 4, 5 (P > 0. 05),,,, 4 1) C 4 0, C 6 0, C 8 0, C 10 0 C 18 2 ; C 12 0, C 14 0, C 16 0, C 18 0 C 18 1, 2), [ ] [ 1 ] So ryal K A, Zeng S S,M in B R, et al. Effect of feeding treatm ents and lactation stages on compo sition and o rgano lep tic quality of goat m ilk Dom iati cheese[j ]. Sm all Rum inant Research, 2004, 52 (1-2) : [ 2 ] Babayan V K. M edium chain length fatty acid esters and their m edical and nutritional app lications[j ]. J Am er O il Chem Soc, 1981, 58: [ 3 ],. [M ]. :, [ 4 ],. [J ]., 1990, 18 (2) : [ 5 ] H a J K, L indsay R C. Release of vo latile branched2chain and o ther fatty acids from rum inant m ilk fats by various lipases[j ]. J D airy Sci, 1993, 76: [ 6 ] Ch illiard Y, Fertay A, Rouel J, et al. A Review of nutritional and physio logical facto rs affecting goat m ilk lip id synthesis and lipo lysis[j ]. J D airy Sci, 2003, (86) : [ 7 ] L edoux M, Rouzeau A, Bas P, et al. O ccurrence of trans2c 18 1 fatty acid isom ers in goat m ilk: effect of two dietary regim ens[j ]. J D airy Sci, 2002, (85) : [8 ] Banskalieva V. Fatty acid compo sition of m ilk fat triacylglycero ls in th ree breeds of goat during lactation [J ]. Comp tes rendue de lπa cadem ie bulgare des Sciences, 2001, 54 (90) : [ 9 ] D eeth H C, F itz2gerald C H, Snow A F. A gas ch rom atograph ic m ethod fo r the quantitative determ ination of free fatty acids in m ilk and m ilk p roducts[j ]. N ew Zealand Journal D airy Sci T echno l, 1983, 18: [ 10 ] T antibhedhyangkul P, H ash im S A. M edium 2chain triglyceride feeding in p rem ature infants: effects on calcium and m agnesium abso rp tion [J ]. Pediatrics, 1978, 61 (4) : [11 ] T antibhedhyangkul P, H ash im S A. M edium 2chain triglyceride feeding in p rem ature infants: effects on fat and nitrogen abso rp tion [J ]. Pediatrics, 1975, 55 (3) : [ 12 ] Schw abe A D, Bennett L R, Bowm an L P. O ctano ic acid abso rp tion and oxidation in hum ans[j ]. J A pp l Physio l, 1964, 19: [ 13 ] Greenberger N J, Sk illm an T G. M edium 2chain triglycerideṡ Physio logic consideration and clinical imp lications[j ]. N Engl J M ed, 1969, 280: [ 14 ] Kalser M H. M edium chain triglycerides[j ]. A dv InternalM ed, 1971, 17: [ 15 ] B L. [M ].,,,. :, P relim inary study on effect of parity on sho rt2 and m edium 2chain fatty acids com po sition in m ilk of X inong Saanen goat L UO Jun, SHAN Cui-yan,L IU La-p ing,w ANG Ha i-bin, SUN X iao-q in,w ANG Xue-q ing,w U Hui- juan (Colleg e of A nim al S cience and T echnology,n orthw est A & F U niversity, Y ang ling, S haanx i , Ch ina) Abstract: F rom the da iry herd of the b reed ing Saanen goa t fa rm in Q ianyang coun ty of Shaanx i p rovince, eigh ty2seven m ilk samp les from Saanen goats of 5 parities w ere co llected to assay the sho rt2 and

5 28 ( ) 33 m edium 2chain fatty acids and m ilk compo sition by u sing gas ch rom atograph and infrared spectrom eter respectively. T he resu lts indicated a sim ilar con ten ts of Butyric (C 4 0), Cap ro ic (C 6 0), Cap rylic (C 8 0), and Cap ric (C 10 0) acids among 5 parities (P > 0. 05) ; how ever, the parity had a sign ifican t effect on con ten ts of L au ric (C 12 0 ), M yristic (C 14 0 ), Palm itic (C 16 0 ), Stearic (C 18 0 ), and O leic (C 18 1 ) acids (P < 0. 05) excep t fo r the non2sign ifican t effect on lino leic (C 18 2) acid con ten t (P > 0. 05) ; there w as no sign ifican t difference in m ilk lacto se con ten t among parities, bu t the sign ifican t effects of parity on fat, p ro tein, dry m atter, and m ilk so lid2non2fat con ten ts in m ilk of X inong Saanen goat w ere ob served (P < 0. 05). Key words: X inong Saanen goat m ilk; fatty acid compo sition; analysis of m ilk compo sition; parity ( 23 ) Abstract ID: (2005) EA A nalysis of genetic differen tiation of Ch inese gam e ch icken by u sing m icro satellite TANG Qing-p ing, CHEN Kuan-we i,l I Hui-fang, ZHANG Shuang- j ie, ZHAO D ong-we i (Institute of P oultry S cience, Chinese A cademy of A g riculture S cience, Yangzhou, J iangsu , China) Abstract: To revea l the genet ic d ifferen t ia t ion of 5 Ch inese gam e ch icken b reed s, the genet ic po lymo rph ism s of 27 m icro satellites of the 5 b reeds w ere studied. M ean heterozygsity, po lymo rph ism info rm ation con ten t,m ean effective num ber of allele and genetic distance w ere calcu lated fo r each b reed and a dendrogram w as ob tained based on genetic distance. T he resu lts show ed that the m ean heterozygou sity in the X ishuangbanna b reed w as the h ighest (0. 662), and that in the H enan w as the low est (0. 532). T he genetic distance betw een the Zhangzhou and the T u rpan w as the farthest (0. 516), and that betw een the L ux i and the X ishuangbanna w as the nearest (0. 206). T he dendrogram show ed that the X ishuangbanna and the T u rpan w ere the descenden t of the L ux i, the reason of w h ich w as that the genetic distance among them w as clo ser. T he genetic distance betw een the Zhangzhou and o ther gam e ch icken s w ere farther. Key words: m icro satellite; Ch inese native gam e ch icken b reeds; genetic diversity; dendrogram