The effect of Kuijieling decoction on the protein expression of I B and IKKβ in colonic mucosa of ulcerative colitis model rats

Transcript

1 Grivennikov SI Karin M. Dangerous liaisons STAT3 and NF-κB ~ Vallabhapurapu S Karin M. Regulation and function of NF-kappa B transcription factors in the immune system. Annu Rev Immunol collab oration and crosstalk in cancer. Cytok Growth ~ Caamano J Hunter CA. NF-κB family of transcription factors central ~ 733 regulators of innate and adaptive immune functions. Clin Microbiol Rev 8 Benitah SA Valeron PF Lacal JC. Rock and nuclear factor kappabdependent ~ 429 activation of cyclooxygenase-2 by Rho GTPases effects on tumor 13 Klement J F Rice N R Car BD. I B deficiency results in a sustained growth and therapeutic consequences. Mol Biol Cell ~ NF-κB response and severe widespread dermatitis in mice. Mol Cell Biol ~ Li Z Zhang DK Yi WQ et al. NF-kappaB p65 antisense oligonucleotides 14. NF-κB may serve as a novel molecular approach for the treatment of patients ~ 1911 with ulcerative colitis. Arch Med Res ~ Gavrilyuk V Dello Russo C Heneka MT. Norepinephrine increase I 10. kappa B alpha expression in astrocytes. J Biol Chem ~ ~ The effect of Kuijieling decoction on the protein expression of I B and IKKβ in colonic mucosa of ulcerative colitis model rats Tang Lihai Ji Yanyan Du Qun Li Yanwu Wu Yanli Chai Yuna Chen Xu Wang Rujun Pi-Wei Institute Guangzhou University of Chinese Medicine Guangzhou Objective To observe the effect of Kuijieling decoction KD on protein expression of I B and IKKβin colonic mucosa of rats with ulcerative colitis UC and to explore its possible mechanism. Methods Trinitrobenzenesulfonic acid TNBS method was used for the establishment of UC rat model. After treatment with different dosages of KD Western blot technique were used to detect protein expression of I B and IKKβin Colonic Mucosa. Results The relative expression of I B protein in model group was obviously lower than that in normal group P < the relative expression of I B protein in high dosage group of KD was significantly higher than that in model group P < the relative expression of I B protein in middle dosage group of KD was obviously higher than that in model group P < The relative expression of IKKβprotein in model group was significantly higher than that in normal group P < the relative expression of IKKβprotein in both high and middle dosage groups of KD were significantly lower than that in model group P < Conclusion The relative expression of I B protein is enhanced and the relative expression of IKKβprotein is Inhibited in colonic mucosa of UC model rats induced by TNBS after treatment with KD Its possible mechanism may inhibit the activation of NF-κB and reduce inflammatory reaction. Key words Kuijieling decoction ulcerative colitis I B IKKβ rat model * 1 1 1** HE * 2009ZX **

2 138 10g /kg 6g /kg centre 3 Thermo Shandon Finesse E + Thermo Fisher % CCl 4 2ml /kg g /kg N- -L g /kg 1 4 DMN DMN 10μl /kg / g /kg 5mg /kg ALT AST Carbon tetrachloride CCl 4 Dimeth- Alb ylnitrosamine DMN Hyp " μg /g " HE 60 60min I 10min 1 II 10min I 5min II 2min 95% 2min 85% 2min 70% 2min 6 5 ~ min 6 3s 4s 95% I 1min 95% 2 II 1min I 1min II 1min I 1min 1 10 OlympusBX Im- Express age-pro N min I -L min II 10min I 5min II 2min 95% 2min 85% 2min 70% 2min C57BL / ± 5 ~ 6 2g Wistar 150 ~ 180g 37 25min SCXK OlympusBX Image-pro Express CCl F DMN KWM Alanine aminotransferase ALT Aspartate aminotransferaseast Albumin Alb Hydroxyproline Hyp P < P < Varifuge 20RS HERAEUS Synergy HT Bio-tek Thermo Shandon Citadel 2000 Thermo Shandon Histo CCl 4 P < DMN P < P < P >

3 139 1 CCl 4 Hyp x 珋 ± s n = 10 P < CCl 4 Hyp g % x 珋 ± s n = ± 0. 95** ± 0. 36** ± 0. 59* ALT AST Alb Hyp ± ± ± ± ± ± 0. g/kg IU /L IU /L g/l μg/g 76** N- -L ± ** ± ** ± ± ** ± ± ± ± ± ± ± * P < ** P < ± * ± * ± ± * 2 DMN N- -L ± ± ± ± x 珋 ± s n = 10 g % ± 0. 43** ± ** ± ± ± ± ± ± ± ± ± ± DMN Hyp x 珋 ± s n = ± ** ± * ± ± ** ± ± ± ± * ALT AST P < Alb P > HE ALT AST P < P < Hyp Hyp P < g/kg ALT IU /L AST IU /L Alb g/l Hyp μg/g ± ** ± ** ± ± ** ± ± ± ± P < CCl 4 100

4 140 4 DMN = 10 CCl 4 x 珋 ± s n 0 I II III IV Ridit ** N- -L ** P < = 10 DMN x 珋 ± s n 0 I II III IV Ridit ** * DMN DMN 3 TGF-β1 -α 4 TGF-β1 CTGF PDGF-BB 5 DMN A 7 TGF-β1 8 3 " " " " " " " " " " 1 Svetlana R Rui S Barbara J et al. Natural killer cells ameliorate liver fibrosis by killing activated stellate cells in NKG2D-dependent and tumor nec- " " " " rosis factor-related apoptosis-inducing ligand-dependent manners. Gastroenterology ~ 452 CCl 4 2 Ala-kokko L Pihlajaniemi T Myers J et al. Gene expression of type I DMN III and Ⅳ collagens in hepatic fibrosis induced by dimethylnitrosamine in the rat. Biochem J ~ ~ 94 ALT AST 4. TNF-α TGFβ ~ ~ CCl ~ HSC-T ~ 1274 DMN 8. A TGF-β ~ 408

5 141 The effects of Fugan decoction on hepatic fibrosis models in mouse and rat Cui Hongyan 1 Gu Weiliang 1 Ma Yueming 1 Liu Ping 2 Liu Chenghai 2 Zhu Bangxian 3 Wang Zhengtao 4 Tao Yanyan 2 1 Department of Pharmacology Shanghai University of Traditional Chinese Medicine Shanghai Institute of Liver Disease Shanghai University of Traditional Chinese Medicine Shanghai Information Center for Life Science of Chinese Academy of Sciences Shanghai University of Traditional Chinese Medicine Shanghai Institute of Traditional Chinese Medicine Shanghai University of Traditional Chinese Medicine Shanghai Objective To evaluate the preventive and therapeutic effects of Fugan Decoction on liver fibrosis induced by carbon tetrachloride CCl 4 in mice and by dimethylnitrosamine DMN in rats. Methods Liver fibrosis in mice was induced by intraperitoneal injection of CCl 4. The rats were randomly divided into 6 groups normal model and Fugan Low Fugan middle Fugan High and N-Acetyl-L-Cysteine control group. All the mice were administrated drugs by garvage for 4 weeks from the the fist CCl 4. injection day. Liver fibrosis in rats was induced by intraperitoneal injection of dimethylnitrosamine DMN. The rats were randomly divided into 6 groups normal model and Fugan Low Fugan middle Fugan High and Perindopril control group. The rats were administered drugs by garvage for 4 weeks after the model was established. The ratios of liver weight /body weight and spleen weight /body weight were calculated. Serum ALT AST activity were determined by colorimetry Alb levels were assayed by bromocresol green method serum hepatic inflammation and collagen deposition were evaluated with HE staining and Sirius-red staining respectively the contents of hepatic hydroxyproline Hyp were measured by alkali digestion method. Results Abnormal liver function obvious hepatic inflammation excess collagen deposition were observed in model group both of mouse and rat compared with those in normal group. Compared to model group Fugan Decoction significantly down-regulated serum ALT and AST activity alleviated liver inflammation it also reduced the level of hepatic hydroxyproline and the pathological excess collagen deposition in liver. Conclusion Fugan decoction has good preventive and therapeutic effects in experimental liver fibrosis. Key words Fugan carbontetrachloride dimethylnitrosamine liver fibrosis collagen deposition * Aβ Aβ CA1 Aβ h 4 Real-Time PCR CDK6 Bax Caspase-3 ELISA Tau Aβ CDK6 Bax Caspase-3 Tau 1. 2g 2. 4g 4. 8g /kg Aβ 1-40 Tau CDK6 Bax Caspase-3 Aβ 1-40 Tau CDK6 Bax caspase3 Aβ 1-40 Alzheimer disease AD 1. 1 Aβ B 1 * 10YZ58 10DZ CB530400