CgIRM S ) , GCg ,,, , R IA [ 1 ], H PL C [ 2, 3 ], GC [ 4, 5 ], GCgM S [ 6, 7 ] M S (S IM ), , :
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1 21 V o l JOU RNAL O F CH IN ESE M A SS SPECTROM ETR Y SOC IET Y N o. 2 GCgCgIRM S Ξ ( ) ( ) GCgCgIRM S,, GCg CgIRM S, ( ) : GCgCgIRM S 1976,, R IA [ 1 ], H PL C [ 2, 3 ], GC [ 4, 5 ], GCgM S [ 6, 7 ], GCg M S, (S IM ),,, GCgM S GCgM S, GCgM S,, (T ge),, : ;,, T ge ; ;, GCgM S,, ( IOC2M S) [ 8, 9, :, ],,, :,, Ξ
2 GCgCgIRM S 13 Cg 12 C, GCgCgIRM S 13 Cg 12 C, 1 GCgCgIRM S GC, CO 2, CO 2, M gz= ,M gz= C 16 O 16 O,M gz= C 16 O 16 O + 12 C 17 O 16 O,M gz= C 18 O 16 O + 12 C 17 O 17 O,,M gz= 44, C 12 C GCg CgIRM S, : = R SPL - R STD 10 3 R STD R, SPL STD, ( ), PDB (Pee D ee Belem n ite), 13 C, R PPB= C 12 C,, 13 Cg 12 C ;, 13 C 13 C, 13 Cg 12 C 10, 13 C 12 C 13 C 12 C,
3 2 GCgCgIRM S 75 2 (T ) 1994,M ichel Becch i Rodrigo A ugu ilera [ 11 ] 12,, C18 Β2 (Β2glucu ron idase), L C, : ; 5Β2-3Α, 17Β- GCgCgIRM S :, ( < - 27 ), 5Β2-3Α, 17Β- ( > - 27 ) : 1996,M ichel Becch i Rodrigo A ugu ilera [ 12 ], 25 : 1),, : ; Β, 172Β2 ; 5Α2 23Α, 17Β2 5Β2 23Α, 17Β2, 2), C18 50: 50,,, 52-3Β, 17Β2 1996,M ichel Becch i [ 13 ], 17Β- H G- T - BSA, D (BSA ), A B ; 3Β- H G- T - BSA, A BSA, D 17Β - H G- T - BSA, : A , A 17Β- H G- T - BSA ; 5Α(Β) 2-3Α, 17Β2 D, D 3Β - H G- T - BSA, GCgM S : 3Β- H G- T - BSA, D 52 23Α, 17Β2, 52 23Α,,, 18Β- H G- T - BSA, M ichel Becch i [ 13 ] :,, GCgCgIRM S, : ; ;, :,
4 %,,, S. Ho rn ing [ 14 ] 40m g, GCgCgIRM S, 10nggmL, 2-20mL S. Ho rn ing , BSA A, D 17Β2OH, 17Β- OH, 5Α (Β) 2-3Α, 17Β2, H PL C 5Α2 23Α, 17Β2 5Β2 23Α, 17Β2 :, 5Α2 23Α, 17Β2 5Β2 23Α, 17Β2, 5Α2 2Α, 17Β2 5Β2 23Α, 17Β2, S. Ho rn ing S. Ho rn ing GCgCgIRM S GCgM S, T ge> 6, 2 4 ;, 10, S. Ho rn ing,,, :, 13 C, 1996, Shack leton [ 10 ] 250m g Girand T, Girand T, 75%, 11,, Sephadex L H - 20 ( 2-3 ) 5Α2 2 3Α, 17Β2 5Β2 23Α, 17Β2, :, 5Α(Β) 2 23Α, 17Β , :, dio l: p reg> 111 Shack leton,, 25mL 5 ; H PL C, 1997, S. Ho rn ing [ 15 ],,, H PL C 5Α2 23Α, 17Β2 5Β2 23Α, 17Β2, GCgCgIRM S 1999, Rodrigo A uguelia [ 16 ] C18, : 1) ; 2) Β2Β2 ( ) ; 3) :, 5Β2 23Α, 17Β2 5Α2 23Α, 17Β2-2612% %, %
5 2 GCgCgIRM S %, 5Β2 23Α, 20Α2,, ( ) 3 (DH T ) 1997, S. Ho rn ing [ 15 ] 25 GCgCgIRM S :, 5Β2 23Α, 17Β2, 5Α2 23Α, 17Β2, 6-16, 5Β2 23Α, 17Β2 5Α2 2 3Α, 17Β2, 70 S. Ho rn ing, 5Β2 23Α, 17Β2, 5Α2 23Α,, S. Ho rn ing GCgM S, : 5Β2 23Α, 17Β2 5Α2 23Α, 17Β2 5ΑAD g5βad > 115, 24 5ΑAD g5βad 1997, Shack leton [ 17 ], GCgCg IRM S, : 1) Girand T Sep 2Pak ; 2) Sodium B ism u thate Shack leton, 5Α2 23Α, 17Β2 5Α2 23Α, 17Α2, 5Β2 23Α, 17Β2, 4 (DH EA ) 1997, S. Ho rn ing [ 15 ] 40m g DH EA, H elix pom atia Β2, H PL C GCgCgIRM S DH EA, 5Α(Β) 2 23Α, 17Β2,,, 52 23Β, 17Β2 5Α(Β) 2 23Α, 17Β Β, 17Β2, Shack leton [ 17 ] 52 23Β, 17Β2 DH EA DH EA 1999,M ako to U ek i [ 18 ] DH EA : Sephadex L H 220 (T EA P Sephadex L H 220) ; Am pullaria ( ) arylsu lfatase ;, GCgCgIRM S ,M ako to U ek i [ 19 ] T EA P Sephadex L H 220, glufatase Β2glucu ron idase, GCgCgIRM S DH EA, 5Α(Β) 2 23Α, 17Β2 DH EA,DH EA 5Α(Β) 23Α,
6 Β2, M ako to U ek i : DH EA 5Α(Β) 2 23Α, 17Β2 DH EA 5, GCgCgIRM S,,,, GCgCgIRM S GCgCgIRM S,,,,,, [ ] GCgCgIRM S GCgM S, GCgCgIRM S GCgM S, GCgCgIRM S,,, GCgCgIRM S,, ( ), GCgCgIRM S,
7 2 GCgCgIRM S 79 1 B rook RV et al. D etection of A nabo lic Stero ids by Radio imm unoassay, B rit J Spo rts M ed 1975, 9: H PL C,, 1989, 24: D arney J r KJ et al. Sim ultaneousm easurem ent of Four T esticular232keto stero ids by Isocratic H igh Perfo rm ance L iquid Ch rom atography w ith on2line U ltravio let A bso rbance D etection, Ibid, 1983, 257: 81 4 U ralets V P et al. A nalysis of A nabo lic Stero ids in Body F luids by Cap illary Gas Ch rom atography w ith a Two- channel D etction System and a Computer, J Ch rom atogr 1983, 279: R A datia et al. A n Impo rtant M ethod fo r Stero id P rofile A nalysis U sing Cap illary gas Ch rom atography, Chrom atographia 1988, 25: Robert M et al. Studies on A nabo lic Stero ids g, B iom ed Environ M ass Spec : Catlin DH et al. A nalytical Chem istry at the XXg rd O lymp ic Gam es in L o s A ngeles, 1984, C lic Chem 1987, 33: Kuoppasalm i K et al. Imp roved D etection of Exogenous T esto sterone in Dop ing A nalysis, In: P roceeding of the 3 rd W o rld Conference on C linical Pharm aco logy and T herapeutics, N o 348, Stockho lm dt al To rre X et al. D etection of T esto sterone E sters in H um an P lasm a, J M ass Spectrom : Cedrie H L Shack leton et al. Confirm ing T esto sterone A dm inistration by Iso tope Ratio M ass Spectrom etric A nalysis of U rinary A ndro stanedio l, Stero ids 1997, 62: BecchiM et al. Gas Chrom atographygcom bustiongiso tope2ratio M ass Spectrom etry A nalysis of U rinary Stero ids to D etect M isuse of T esto sterone in Spo rt, Rap id Comm unications in M ass Spectrom etry 1994, 8: A guilera R et al. Imp roved M ethod of D etection of T esto sterone A buse by Gas Ch rom atographygcom bustiongiso tope Ratio M ass Spectrom etry A nalysis of U rinary Stero ids, J M ass Spectrom 1996, 31: A guilera R et al. D etection of T esto sterone M isuse: Comparison of Two Ch rom atograph ic Samp le P reparation M ethods fo r Gas Ch rom atograph ic2com bustiongiso tope Ratio M ass Spectrom etric A nalysis, J Ch rom atogr B 1996, 687: S Ho rning et al. D etection of Exogenous T esto sterone by 13 Cg 12 C A nalysis. In: P roceedings of the 14 th Co logne W o rk shop on Dop ing A nalysis, 17 th to 22 nd M arch 1996,W Schaenzer et al. eds, Spo rt und Buch Strausse Edition Spo rt, Koeln 1997, S Ho rning et al. D etection of Exogenous Stero ids by 13 Cg 12 C A nalysis. In: P roceedings of the 15 th Co lognew o rk shop on Dop ing A nalysis, 23 th to 28 th February 1997, W Schaenzer et al. eds, Spo rt und Buch Strausse Edition Spo rt, Koeln 1998, A guilera R et al. Screening U rine fo r Exogenous T esto sterone by Iso tope Ratio M ass Spectrom etric A nalysis of O ne P regnanedio l and Two A ndro stanedio ls, J Ch rom atogr B 1999, 727: Cedrie H L Shack leton et al. A ndro stanedio l and 52A ndro stenedio l P rofiling fo r D etecting Exogenously A dm inistered D ihydro testo sterone, Ep itesto sterone, and D ehydroep iandro sterone: Po tential U se in Gas Chrom atography Iso tope Ratio M ass Spectrom etry, Stero ids 1997, 62: M ako to U ek i et al. Dop ing W ith N aturally O ccuring Stero ids, J Toxical- Toxin Review s 1999, 18 (2) : M ako to U eki et al. A nalysis of Exogenous D ehydroep iandro sterone Excretion in U rine by Gas Chrom atographyg Com bustiongiso tope Ratio M ass Spectrom etry, Rap id Comm unications in M ass Spectrom etry 1999, 13: 2237
8 Recen t D evelopm en t on the Application of Gas Chroma tographygcom bustiongisotop ic Ra tio M a ss Spectrom etry to D etecting Endogenous Anabol ic Androgen ic Stero ids W a Chun ling,w u M ou tian, Zhang Changjiu (Ch ina Dop ing Con tro l Cen tre,beijing , Ch ina) R eceived Abstract Gas Ch rom atographygcom bu stiongiso top ic R atio M ass specrom etry has p layed an im po rtan t part in detecting endogenou s anabo lic androgen ic stero idṡ T he p rincip le of Gas Ch rom atographygcom bu stiongiso top ic R atio M ass spectrom etry and a review on recen t developm en t on the app lication of it to detecting endogenou s anabo lic androgen ic stero ids are p resen ted. Key W o rds: GCgCgIRM S, endogenou s anabo lic androgen ic stero ids, app lication
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