Ana lysis of the Com ponen t and F ingerpr in t of
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- Αφροδίσιος Ζωγράφος
- 8 χρόνια πριν
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1 Journal of Ch inese M ass Spectrom etry Society V o l. 25 N o. 3 A ug , 3, (, ) : 2 (GCgM S), N IST GCgM S,, : 22, 16, : ; ; 2 (GCgM S); : O ; Q : A : (2004) Ana lysis of the Com ponen t and F ingerpr in t of Effective Part of L igusticum Chuanx iong Horṫ by Ga s Chroma tography-m a ss Spectrom etry L IAN G M ing2jin, H E L ang2chong 3,L I Yong2m ao (Colleg e of P harm acy, X i, an J iaotong U niversity, X i, an , Ch ina) Abstract: T he com ponen ts of lipoph ilic part of L igu sticum Chuanx iong Ho rṫ (L CH ) w ere analyzed by u se of gas ch rom atographygm ass spectrom eter (GCgM S) w ith N IST lib rary. U nder the ch rom atograph ic condition s, the sam p les of L CH from differen t p lace w ith differen t batch w ere m easu red and the comm on peak s in the fingerp rin t w ere confirm ed. Tw en ty tw o com pounds in lipoph ilic part of L CH w ere iden tified and six teen of them w ill be u sed as comm on peak s of the fingerp rin t to con tro l the quality of L CH. T he fingerp rin t w ith active com pounds can basically illu strate the com po sing character of lipoph ilic part of L CH and the quality of L CH can be con tro lled by th is m ethod. Key words: m a ss sp ect rom et ry; study on L igu st icum Chuanx iong Ho rṫ ; ga s ch rom atography2m ass spectrom etry (GCgM S) ; effective part; fingerp rin t : ; : : : ( ) : (1975 ), ( ),,, E2m ail: L iangm j@m ailsṫ xjtu. edu. cn 3 : (1957 ), ( ),,, E2m ail: helc@m ailsṫ xjtu. edu. cn
2 3 : (R h izom a Chuanx iong) (E I) 200 ; L igu sticum chuanx iong Ho rṫ, (T IC) ; 1. 0 ΛL, 1. 2 ml gm in; [ 1, ], 2. 5 m in, AD P 114, [ 2 4 ] [ 5 7 ] m g, 50 ml, 1 ml m g, ,, 20, 0. 5 kg, 1 L CO 2 [ 8 10 ],, GCgM S,, [ ] M Pa, 40, 40, kggh 3 h,,, g, GCgM S, 3. 72% 40 m g, 10 ml,,,, GCgM S GCgM S2Q P : 1. 0 ΛL, Sh im adzu, GCgM S so lu tion, GCgM S N IST ; DB 25M S ( : 30 m mm, Λm, 1),, A gilen t ( ) ; HA (Αi), : (A i% ) ( : ) : M eker ;, 1. 0 ΛL GCgM S :, GCgM S, % 16 (Αi) R SD ( : 0. 1% ), ( : ) 6, 1. 0 ΛL GCg (L igu sticum Chuanx iong Ho rṫ ) ; M S, 16 (L igu stilide) :, (Αi) R SD 0. 1% (H PL C) > 98% DB 25M S ( 30 m mm, Λm ),, 16 Αi R SD : 80, 2 m in, 10 gm in 250, 11 m in; ( : ) 1, h 1. 0 ΛL GCgM S, 0. 1% ; , 12 : ; 50; 250 ;,
3 a (L igustilide) ; b (effective part of L CH ) GCgM S, Αi A i%,,,, GCgM S 1,,, [ ],,, 1 (a), GCgM S (b) GCgM S, F ig. 1 Chromatogram s of L igustilide (a) and effective part of LCH (b) by GCgM S,, 212 [ 5 7 ] CO 2,, 0. 5%, Αi A i% 9 CO 2 (80% ), ( 0. 1% ) 16, CO , 12 ( 16 98% ), GCgM S, tr m in, 16 2,,, (m gz, % ) : 190 (M +, 66. 3) 161 (M + 2C 2H 5, 95. 4) 148 (M + 2C 3H 6, 94. 9) 133 (M + 2C 3H 5O, 24. 1) 105 (M + 2C 4H 6O 2, 76. 5) 77 (M + 2C 6H 9O 2, ) N IST [ 17, ] CO 2,,,,,,,, (40 ),, 213, GCgM S GCgM S,,
4 3 : , GCgM S , (R SD % ) < 0. 1%,, 16,, 12 1 Table 1 Analytical result of the com ponen ts in effective part of LCH gd a N o. compound trgm in mo lecular mo lecular fo rm ula w eigh t 1 dim ethyl phthalate C10H 10O eudesm a24 (14), 112diene C15H , 52 3, 52ditertbutyl2pheno l C15H diethyl phthalate C12H 14O decane C14H (2, 42 ) 212 [ 12(2, 42dim ethylphenyl) 2 12p ropanone ] C11H 14O butylidene phthalide C12H 12O , 42 62butyl21, 42cyclohep tadiene C11H Α2 Α2butyl2benzenem ethano l C11H 16O m ethyl222pyridinam ine C6H 8N ligustilide C12H 14O , 7, 82 5, 7, 82trim ethyl2dihydrocoum arin C12H 14O tetradecano ic acid C14H 28O dibutyl phthalate C16H 22O palm itic m ethyl ester C17H 34O hep tadecano ic acid C17H 34O hexadecano ic ethyl ester C18H 36O , 122 9, 122octadecadieno ic acid m ethyl ester C19H 34O octadecadieno ic acid m ethyl ester C19H 36O , 122 9, 122octadecadieno ic acid C18H 32O octadecadieno ic acid C18H 34O octadecano ic acid C18H 36O GCgM S F ig. 2 M ass spectrum of L igustilide by GCgM S : [1 ] [S ] ( ): 37. [2 ],,,. [J ]., 1999, 15 (3): [ 3 ] L im in, Shunno suke H anda, Yasuo Ikeda, et al. Specific Inh ib iting Characteristics of T etram ethylpyrazine, O ne of the A ctive Ingredien ts of the Ch inese H erbal M edicine Chuanxiong on P latelet T h rom bus Fo rm ation U nder H igh Shear R ates [ J ]. T h rom bo sis R esearch, 2001, 104 : [4 ] T saia Ch inchuan, L ai T ungyuan, H uang W eichan, et al. Inh ib ito ry Effects of Po tassium Channel B lockers on T etram ethylpyrazine2induced R elaxation of R at A o rtic Strip in V itro [J ]. L ife
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