CR F (Co rtico trop ing2 releasing facto r) N E, ,, g, , SPA Tsinghua Tongfang Optical Disc Co., Ltd. All rights reserved.

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1 1997, 17 (2): A cta T heriolog ica S inica Ξ (,, ) CR F N E (O chotona cu rz oniae) : CR F 1 Λg, 2912% (P < 0101), CR F Α2helical CR F2 (9241) 50 Λg CR F 1 Λg CR F ; 5 nm N E,, 38185% (P < 0101), 62OHDA 24131% (P < 0101), CR F, ; ; IgG ;,,, CR F (Co rtico trop ing2 releasing facto r) CR F,, (O chotona cu rz on iae),,, CR F CR F N E, 62 (62OHDA ), 11,, g,, I2N a, SPA, N unc2imm uno T ube (StarT ube) D enm ark N unc, 62OHDA Ξ ( ) ,

2 sigm a, CR F Α2heilical CR F (941) Pen isu la, Sep hadex G250Pharm acia 31, 62OHDA 012%, 4 24 h 41SPA 125 I 115 m l SPA 50 Λl (1 m ggm l) 125 I2N a 1 m C i T 20 Λl (1 m ggm l), 1 m in, N a3s2o 420 Λl (2 m ggm l), Sephadex G250, 011 M PBS (015 m lgm in), 1 tubeg2 m in 51 (100 Λggm l) 100 Λl Star T ube 8 h, 011 M PBS 015% BSA PBS 200 Λl 4 h, 0105% T riton X2100PBS, 100 Λl, 100 Λl PBS 4, PBS, 125 I2SPA 100 Λl ( cpm ) 4, PBS, Χ2 cpm, 125 I2cpm 61, I2SPA SPA , Fc, 125 I SPA 125 I2SPA 1, 125 I2SPA I2SPA Sephadex G250 F ig. 1P rofile of 125 I 2SPA on Sephadex G250 co lum n w ith 011 mo lgl PBS ph

3 21 N E 62OHDA 3,, N E 5 nm, 62OHDA 100 Λg, 5 Λl 5 Λggkg, 62OHDA 7 d 3 14 d,, : 5 nm N E, 38185% (P < 0101), 100 Λg 62OHDA 7 d 24131% (P < 0101), (2) 2 N E 62OHDA IgG F ig. 2Effect of icv adm inistration of N E and 62OHDA on p roduction of IgG antibody (n= 810, 3 3 P < 0101) 3 CRF CRF Α2helical CRF2 (9241) IgG F ig. 3Effect of icv adm inistration of CRF and Α2helical CRF2 (9241) on p roduction of IgG antibody (n= 810, 3 3 P < 0101) 31 CR F CR F Α2helical CR F2 (9241) CR F1 Λg,, 2912% (P < 0101), CR F Α2helical CR F2 (9241) 50 Λg CR F1 Λg, CR F (3) 133

4 , CR F, Α2helical CR F2 (9241) CR F, CR F, : 1. CR F A CTH (GC), ; CR F A CTH N K (Strau sbaugh, 1992; Jain, 1991) ; 2. CR F N E N eu ropep tide2y ( Irw in, 1992), CR F N E E (B row n, 1982), CR F, ( Irw in, 1988) N E (Felten, 1988; L ivnat, 1985), N E Β2 N K (H ellstrand, 1985), Β2 CR F ( Irw in, 1990), 1 (in terleuk in21, IL 21) (Sundar, 1990), (Cunn ick, 1990) CR F,, PVN CR F, CR F (P lo tsky, 1989; Gu illem in, 1987) PVN CR F Α1 (P lo tsky, 1987),, Α1Β N E E CR F (Szafarczyk, 1987),, N E,,,, N E CR F B row n M R, F isher LA, Sp iess J, R ivier C, R ivier J, V ale W Co rtico trop in releasing facto r: actions on the sympathetic nervous system and m etabo lism. E nd ocinology, 111: Cunnick JE, L ysle D T, KucinskiBJ, Rabin BS Evidence that shock2induced imm une supp ression is m ediated by adrenal ho rmones and peripheral Β2adrenergic recep to rs. P harm acol B iochem B ehav, 36: Felten SY, Felten DL, Bellinger DL, Carlson SL, A ckerm an KD, M adden KS, O lschow ka JA, L ivnat S. drenergic sympathetic innervation of lympho id o rgans. P rog A llergy, 43: N o ra2 Guillem in V, Conte2D evo lx B, Szafarczyk A, M alaval F, Gibauld R The co rtico trop in2releasing facto r release in rat hypophysial po rtal blood is m ediated by brain catecho lam ines. N eu roend ocrinol, 46: H ellstrand K, H ermodsson S, Strannegard O Evidence fo r a Β2adrenocep to r2m ediated regulation of hum an natu2 ral k iller cells. J Imm unol, 134: Irw in M, H auger R, B row n M Central co rtico trop in releasing ho rmone activates the sympathetic nervous sys2 tem and reduces imm une function: increased responsivity of the aged raṫ E nd ocrinology, 131: Irw in M, H auger RL, Jones L, P rovencio M, B ritton KT Sympathetic nervous system m ediates central co rti2 134

5 co trop in2releasing facto r induced supp ression of natural k iller cyto toxicity. J P harm acol E xp T her, 255: Irw in M, H auger RL, B row n M, B ritton KT CRF activates autonom ic nervous system and reduces natural k iller cyto toxicity. A m J P hy siol, 255: R 744R 747. Jain R, Zw ick ler D, Ho llander CS, B rand H, Saperstein A, H utch inson B, B row n C, A udhya T Co rtico trop in2 releasing facto r modulates the imm une response to stress in the raṫ E nd ocrinology, 128: L ivnat S, Felten SY, Carlson SL, Bellinger DL, Felten DL Invo lvem ent of peripheral and central catecho lam ine system in neural2imm une interactions. J N eu roimm unol, 10: 530. P lo tsky PM, Cunningham ET, W idm aier EP Catecho lam inergic modulation of co rtico trop in2releasing facto r and adrenoco rtico trop in secretion. E nd ocr R ev, 10: P lo tsky PM Facilitation of imm uno reactive co rtico trop in2releasing facto r secretion in to the hypophysial2po rtal circulation after activation of catecho lam inergic pathw ays o r central no rep inephrine injection. E ndocrinology, 121: Strausbaugh H, Irw in M Central co rtico trop in2releasing ho rmone reduces cellular imm unity. B rain B ehav Im 2 m unol, 6: Sundar SK, C ierp ialm A, Kilts C, R itch ie JC,W eiss JM IL 21 induced imm uno supp ression occurs though activa2 tion of p ituiary2adrenal axis and sympathetic nervous system by co rtico trop in2releasing facto ṙ J neu rosci, 10: Szafarczyk A, M alaval F, L aurent A, Gibauld R Further evidence fo r a central stim ulato ry action of cate2 cho lam ines on adrenoco rtico trop in release in the raṫ E nd ocrinology, 121: THE INH IB ITORY EFFECT OF CORT ICOTROPH IN REL EASING FACTOR AND NOREP INEPHR INE ON HUMORAL IMM UNE FUNCT ION OF OCH TON A CUR ZON IA E BA I H aibodu J izeng (N orthw est P lateau Institute of biology, the Chinese A cademy of S ciences, X ining, ) Abstract W hat action the CR F and N E p layed in m odu lating imm une function in the b rain of O chotona cu rz on iae w as studied. T he changes of hum o ral imm une function of O chotona cu rz on iae w ere exam ined by icv in jection of CR F, N E and their b lockeṙ T he resu lts show ed that icv CR F (110 Λg) resu lted in a 2912% (P < 0101) decrem en t in the p roduc2 tion of IgG; Cen tral adm in istration (icv) of the CR F an tagon ist 2helical CR F2 (9241) 50 Λg com p letely b locked the imm uno supp ressive action of CR F, w hen an im al w ere icv in2 jection of N E 5 nm p roduced a sign ifican t supp ression of the IgG p roduction. A fter de2 stroyed cen tral sym pathetic nervou s system w ith 62OHDA, the IgG levels increased by 2413% (P < 0101). T hese findings suggest that enhanced CR F level in the b rain m igh t be a sou rce to reduce hum o ral imm une function and cen tral sym pathetic nervou s system m ay p lay a strained supp ressive ro le in m odu lation of hum o ral imm une in O chotona cu r2 z on iae. Key wordsco rtico troph in releasing facto r; N o rep ineph rine; IgG an tibody; Im 2 m uno regu lation 135

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